公开(公告)号：US11407792B2

公开(公告)日：2022-08-09

申请号：US16186226

申请日：2018-11-09

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Jonathan Silberg ,  Joshua T. Atkinson ,  Ian J. Campbell ,  George N. Bennett

IPC分类号： C07K14/21 ,  G01N33/566 ,  C07K14/415 ,  G01N33/542

摘要： Engineered protein electron carriers, microorganisms expressing the same, and methods detecting regulated electron flow are described.

公开(公告)号：US10752925B2

公开(公告)日：2020-08-25

申请号：US16015101

申请日：2018-06-21

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu San ,  George N. Bennett ,  Irene Martinez

IPC分类号： C12P7/46 ,  C12N9/88

摘要： Microbes and methods used to convert renewable carbon sources such as glucose, sucrose, biomass hydrolysate, methanol or formate, to succinate-derived products, such as fumarate or malate, which are desirable products having many uses.

公开(公告)号：US09856499B2

公开(公告)日：2018-01-02

申请号：US14355402

申请日：2012-12-20

申请人： William Marsh Rice University

发明人： George N. Bennett ,  Xianpeng Cai

IPC分类号： C12P7/52 ,  C12N1/20 ,  C12N1/38 ,  C12P7/40

CPC分类号： C12P7/52 ,  C12N1/20 ,  C12N1/38 ,  C12P7/40

摘要： Method of cell culture, comprising adding a redox active compound with a redox potential of between −0.116 to −0.253 to a culture capable of forming hydrogen via a hydrogenase so that the redox potential is diverted from hydrogen to form a longer chain acids, e.g., butryic acid.

公开(公告)号：US20190135878A1

公开(公告)日：2019-05-09

申请号：US16186226

申请日：2018-11-09

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Jonathan Silberg ,  Joshua T. Atkinson ,  Ian J. Campbell ,  George N. Bennett

IPC分类号： C07K14/21 ,  G01N33/566

摘要： Engineered protein electron carriers, microorganisms expressing the same, and methods detecting regulated electron flow are described.

公开(公告)号：US20180371508A1

公开(公告)日：2018-12-27

申请号：US16015101

申请日：2018-06-21

申请人： WILLIAM MARSH RICE UNIVERSITY

发明人： Ka-Yiu SAN ,  George N. Bennett ,  Irene Martinez

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  C12N9/88 ,  C12Y402/01002 ,  C12Y604/01001

摘要： Microbes and methods used to convert renewable carbon sources such as glucose, sucrose, biomass hydrolysate, methanol or formate, to succinate-derived products, such as fumarate or malate, which are desirable products having many uses.

公开(公告)号：US10011839B2

公开(公告)日：2018-07-03

申请号：US15263146

申请日：2016-09-12

申请人： William Marsh Rice University

发明人： Ka-Yiu San ,  George N. Bennett ,  Hui Wu

IPC分类号： C12N9/10 ,  C12N15/52 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P13/14 ,  C12P13/06 ,  C12P13/08 ,  C12P7/56 ,  C12N9/80 ,  C12P1/04 ,  C12P7/06 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P13/04 ,  C12P13/18 ,  C12P13/22

CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12P1/04 ,  C12P7/065 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/14 ,  C12P13/18 ,  C12P13/222 ,  C12P13/225 ,  C12Y102/01025 ,  C12Y102/04004 ,  C12Y102/07007 ,  C12Y103/01076 ,  C12Y103/05005 ,  C12Y103/99031 ,  C12Y114/13027 ,  C12Y205/01001 ,  C12Y205/01029 ,  C12Y205/01032 ,  C12Y205/01093 ,  C12Y305/01022 ,  C12Y305/99002 ,  C12Y401/01024 ,  C12Y499/01004 ,  C12Y602/01011 ,  Y02E50/17

摘要： The disclosure relates to a metabolic transistor in microbes such as bacteria and yeast where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the microbe.

公开(公告)号：US09441253B2

公开(公告)日：2016-09-13

申请号：US14176008

申请日：2014-02-07

申请人： William Marsh Rice University

发明人： Ka-Yiu San ,  George N. Bennett ,  Hui Wu

IPC分类号： C12P13/22 ,  C12P7/56 ,  C12P7/54 ,  C12P13/18 ,  C12P13/08 ,  C12P13/06 ,  C12N15/52 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88 ,  C12N9/00 ,  C12P1/04 ,  C12P7/06 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P13/04 ,  C12P13/14

CPC分类号： C12P13/225 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/80 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P1/04 ,  C12P7/065 ,  C12P7/18 ,  C12P7/40 ,  C12P7/46 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/14 ,  C12P13/18 ,  C12P13/222 ,  C12Y102/01025 ,  C12Y102/04004 ,  C12Y102/07007 ,  C12Y103/01076 ,  C12Y103/05005 ,  C12Y114/13027 ,  C12Y205/01001 ,  C12Y205/01032 ,  C12Y205/01093 ,  C12Y305/01022 ,  C12Y305/99002 ,  C12Y401/01024 ,  C12Y602/01011 ,  Y02E50/17 ,  Y02P20/52

摘要： The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.

摘要翻译： 本公开涉及细菌中的代谢晶体管，其中引入竞争性途径以与可用碳的产物途径竞争，以便控制细菌中的碳通量。

公开(公告)号：US20150044743A1

公开(公告)日：2015-02-12

申请号：US14355402

申请日：2012-12-20

申请人： William Marsh Rice University

发明人： George N. Bennett ,  Xianpeng Cai

IPC分类号： C12P7/52

CPC分类号： C12P7/52 ,  C12N1/20 ,  C12N1/38 ,  C12P7/40

摘要： Method of cell culture, comprising adding a redox active compound with a redox potential of between −0.116 to −0.253 to a culture capable of forming hydrogen via a hydrogenase so that the redox potential is diverted from hydrogen to form a longer chain acids, e.g., butryic acid.

摘要翻译： 细胞培养方法包括向氧化还原电位为-0.116至-0.253之间的氧化还原活性化合物加入能够通过氢化酶形成氢的培养物，使得氧化还原电位从氢转移而形成较长链的酸， 丁酸。

公开(公告)号：US08709753B2

公开(公告)日：2014-04-29

申请号：US13680608

申请日：2012-11-19

申请人： William Marsh Rice University

发明人： Ka-Yiu San ,  George N. Bennett ,  Yipeng Wang

IPC分类号： C12N15/70

CPC分类号： C12N15/70 ,  C12N9/0008 ,  C12N9/1205 ,  C12P5/007 ,  C12P7/52 ,  C12P17/08 ,  C12P19/36 ,  C12Y101/01008 ,  C12Y101/01094 ,  C12Y207/01023

摘要： This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.

摘要翻译： 本发明是为提高NADPH依赖化合物的产量和生产力而提供增加的细胞内NADPH可用性的代谢工程菌株。 在本发明中，天然NAD依赖性GAPDH被NADP依赖性GAPDH加上过表达的NADK替代。 也提供了细菌的使用。