公开(公告)号：US20080156078A1

公开(公告)日：2008-07-03

申请号：US11902376

申请日：2007-09-21

申请人： Wen-Hsin Hsieh ,  Meng-Shiun Tsai ,  Oscal Tzyh-Chiang Chen ,  Song-Jeng Huang ,  Tin-Kwang Lin ,  Yu-Wen Huang

发明人： Wen-Hsin Hsieh ,  Meng-Shiun Tsai ,  Oscal Tzyh-Chiang Chen ,  Song-Jeng Huang ,  Tin-Kwang Lin ,  Yu-Wen Huang

IPC分类号： G01N11/00 ,  B06B1/00 ,  G01H17/00

CPC分类号： G01N29/022 ,  G01N29/07 ,  G01N29/075 ,  G01N2291/02466 ,  G01N2291/0423

摘要： A method of test includes the steps of preparing a sensing platform having an emitting electrode mounted at on one side thereof and a receiving electrode mounted on the other side thereof, wherein the sensing platform defines a sensing zone located between the emitting and receiving electrodes; placing a specimen on the sensing zone; emitting a surface acoustic wave from the emitting electrode, wherein the surface acoustic wave passes through the sensing zone and the specimen and then is received by the receiving electrode to be changed for its speed and phase by the change of material property of the specimen; and identifying the material property of the specimen according to the changed speed and phase of the surface acoustic wave to further infer the physical property of the specimen. In light of the steps, the surface acoustic wave can be employed for detection of the physical property of the specimen.

摘要翻译： 一种测试方法包括以下步骤：制备具有安装在其一侧上的发射电极的感测平台和安装在其另一侧上的接收电极，其中感测平台限定位于发射电极和接收电极之间的感测区域; 将样品置于感应区; 从所述发射电极发射表面声波，其中所述表面声波通过所述感测区域和所述样本，然后被所述接收电极接收，以通过所述样本的材料特性的变化而改变其速度和相位; 根据表面声波的变化的速度和相位来识别样品的材料特性，以进一步推断样品的物理性质。 根据这些步骤，可以使用表面声波来检测样品的物理性质。

公开(公告)号：US20090187350A1

公开(公告)日：2009-07-23

申请号：US12353635

申请日：2009-01-14

申请人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu

发明人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu

IPC分类号： G01N33/48 ,  G02B5/18

CPC分类号： G01N21/554 ,  G01N21/253 ,  G01N21/4788 ,  G01N21/7743 ,  Y10S977/701 ,  Y10S977/834 ,  Y10S977/932

摘要： A bio-sensing system comprises a light source, a bio-sensing apparatus, a detecting platform, and a processing unit. A bio-sensing apparatus further comprising a substrate, a sample with at least one analyte, at least one grating bound on the substrate for diffracting a light beam in a reflection mode and outputting at least one output light beam, a plurality of nanoparticles being bound on one side of the grating, a molecular recognition unit bound on said nanoparticle surface, and a cover plate covering the nanoparticle-modified side of the substrate. The detecting platform receives a signal while the at least one output light beam passing through the bio-sensing apparatus. The processing unit couples with the detecting platform for receiving and analyzing the signal. Wherein when the analyte passes through the microfludic channel to contact with said nanoparticles, the at least one output light beam changes in accordance with the refractive index of the sample or in accordance with the interaction of the analyte with said molecular recognition unit bound on said nanoparticle surface.

摘要翻译： 生物感测系统包括光源，生物感测装置，检测平台和处理单元。 一种生物传感装置，其进一步包括基底，具有至少一种分析物的样品，结合在所述基底上的至少一个光栅，用于以反射模式衍射光束并输出至少一个输出光束，多个纳米粒子被束缚 在光栅的一侧上，结合在所述纳米颗粒表面上的分子识别单元和覆盖基板的纳米粒子改性侧的覆盖板。 所述检测平台在所述至少一个输出光束通过所述生物感测装置的同时接收信号。 处理单元与检测平台耦合，用于接收和分析信号。 其中当分析物通过微流体通道以与所述纳米颗粒接触时，所述至少一个输出光束根据样品的折射率或根据分析物与结合在所述纳米颗粒上的所述分子识别单元的相互作用而变化 表面。

公开(公告)号：US08305583B2

公开(公告)日：2012-11-06

申请号：US12647251

申请日：2009-12-24

申请人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu ,  Po-Liang Chen

发明人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu ,  Po-Liang Chen

IPC分类号： G01N21/55

CPC分类号： G01N21/554 ,  B01L3/5027 ,  B82Y15/00 ,  B82Y20/00 ,  G01N21/7703 ,  G02B6/0229

摘要： A sensing system comprises a light source, an optical fiber, a plurality of noble metal nano-particles, a micro-fluidic module and a photo detector. The optical fiber couples an incident light. The plurality of noble metal nano-particles are disposed on a surface of the optical fiber to form a noble metal nano-particle submonolayer, the noble metal nano-particles are substantially separated from each adjacent noble metal nano-particles such that the conductivity of the noble metal nano-particle submonolayer is smaller than that of a metal film. The micro-fluidic module accommodates the optical fiber and a sample, and the sample is driven to contact with the noble metal nano-particles. The photo detector detects an emergent light from the optical fiber. When the incident light interacts with the noble metal nano-particles, a signal derived from localized surface plasmon resonance in form of attenuated light or elastic scattered light is outputted through the photo detector.

摘要翻译： 感测系统包括光源，光纤，多个贵金属纳米颗粒，微流体模块和光电检测器。 光纤耦合入射光。 多个贵金属纳米粒子配置在光纤的表面，形成贵金属纳米粒子单层，贵金属纳米粒子与各相邻的贵金属纳米粒子基本上分离， 贵金属纳米颗粒亚单层比金属膜小。 微流体模块容纳光纤和样品，驱动样品与贵金属纳米颗粒接触。 光检测器检测来自光纤的紧急光。 当入射光与贵金属纳米粒子相互作用时，通过光电检测器输出以衰减光或弹性散射光形式的局部表面等离子体共振信号。

公开(公告)号：US08068995B2

公开(公告)日：2011-11-29

申请号：US12353635

申请日：2009-01-14

申请人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu

发明人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu

IPC分类号： G02B5/18 ,  G02B5/00 ,  G02B1/10 ,  G02B1/00 ,  G02F1/01

CPC分类号： G01N21/554 ,  G01N21/253 ,  G01N21/4788 ,  G01N21/7743 ,  Y10S977/701 ,  Y10S977/834 ,  Y10S977/932

摘要： A bio-sensing system comprises a light source, a bio-sensing apparatus, a detecting platform, and a processing unit. A bio-sensing apparatus further comprising a substrate, a sample with at least one analyte, at least one grating bound on the substrate for diffracting a light beam in a reflection mode and outputting at least one output light beam, a plurality of nanoparticles being bound on one side of the grating, a molecular recognition unit bound on said nanoparticle surface, and a cover plate covering the nanoparticle-modified side of the substrate. The detecting platform receives a signal while the at least one output light beam passing through the bio-sensing apparatus. The processing unit couples with the detecting platform for receiving and analyzing the signal. Wherein when the analyte passes through the microfludic channel to contact with said nanoparticles, the at least one output light beam changes in accordance with the refractive index of the sample or in accordance with the interaction of the analyte with said molecular recognition unit bound on said nanoparticle surface.

摘要翻译： 生物感测系统包括光源，生物感测装置，检测平台和处理单元。 一种生物传感装置，其进一步包括基底，具有至少一种分析物的样品，结合在所述基底上的至少一个光栅，用于以反射模式衍射光束并输出至少一个输出光束，多个纳米粒子被束缚 在光栅的一侧上，结合在所述纳米颗粒表面上的分子识别单元和覆盖基板的纳米粒子改性侧的覆盖板。 所述检测平台在所述至少一个输出光束通过所述生物感测装置的同时接收信号。 处理单元与检测平台耦合，用于接收和分析信号。 其中当分析物通过微流体通道以与所述纳米颗粒接触时，所述至少一个输出光束根据样品的折射率或根据分析物与结合在所述纳米颗粒上的所述分子识别单元的相互作用而变化 表面。

公开(公告)号：US20090283406A1

公开(公告)日：2009-11-19

申请号：US12382882

申请日：2009-03-26

申请人： Wen-Hsin Hsieh ,  Lai-Kwan Chau

发明人： Wen-Hsin Hsieh ,  Lai-Kwan Chau

IPC分类号： G01N27/26

CPC分类号： B03C5/005 ,  B03C5/022

摘要： A method and an apparatus for detection of a bioparticle by single-bead based DEP. The method includes the steps of immobilizing a single DEP bead in one electric field; immobilizing a first bio-recognizing molecule on the single DEP bead; intromitting at least one target bioparticle into the electric field for binding the first bio-recognizing molecule, whereby the target bioparticle and the first bio-recognizing molecule are bound with each other to form a complex molecule; and detecting the complex molecule by a detection device. The apparatus is composed of a chip, a power source, a single DEP bead, and a detection device.

摘要翻译： 用于通过基于珠粒的DEP检测生物颗粒的方法和装置。 该方法包括在一个电场中固定单个DEP珠粒的步骤; 将第一生物识别分子固定在单个DEP珠上; 将至少一种目标生物粒子引入电场以结合第一生物识别分子，由此靶生物颗粒和第一生物识别分子彼此结合以形成复合分子; 并通过检测装置检测复合分子。 该装置由芯片，电源，单个DEP珠和检测装置组成。

公开(公告)号：US20090190877A1

公开(公告)日：2009-07-30

申请号：US12359080

申请日：2009-01-23

申请人： Shau-Chun Wang ,  Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Chia-Yu Lee

发明人： Shau-Chun Wang ,  Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Chia-Yu Lee

IPC分类号： G02B6/00 ,  G01J1/58

CPC分类号： G01N21/7703 ,  G01N21/554 ,  G01N2021/7736 ,  G01N2021/7783

摘要： A microfluidic device with microstructure includes a channel for accommodating an electrolytic solution therein and at least one microstructure formed in the channel. When an alternating-current signal is input to the microfluidic device so that a surface of the microstructure is polarized by a generated electric field, ions having polarity reverse to that of an electrolytic solution will migrate to the surface of the microstructure to form a field-induced electrical double layer to result in electro-osmotic flows at the corners at two sides of the microstructure, which causes formation of relatively fierce circular vortices in the solution. A sensing system and a sensing method using the microfluidic device with microstructure are also disclosed.

摘要翻译： 具有微结构的微流体装置包括用于容纳其中的电解溶液的通道和在通道中形成的至少一个微结构。 当将交流信号输入到微流体装置以使得微结构的表面被产生的电场极化时，具有与电解液相反极性的极性的离子将迁移到微结构的表面， 导致电双层，导致微观结构两侧角落处的电渗流，这导致在溶液中形成相对激烈的圆形涡流。 还公开了一种使用具有微结构的微流体装置的感测系统和感测方法。

公开(公告)号：US20080019876A1

公开(公告)日：2008-01-24

申请号：US11612950

申请日：2006-12-19

申请人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Po-Liang Chen ,  Kaua-Ciih Chiu ,  Wei-Ting Hsu

发明人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Po-Liang Chen ,  Kaua-Ciih Chiu ,  Wei-Ting Hsu

IPC分类号： G01N21/00

CPC分类号： G01N21/554

摘要： This invention discloses a sensing apparatus with noble metal and sensing system and its method. The sensing apparatus with noble metal comprises a planar waveguide, a noble metal nano-particle layer, and a cover. The planar waveguide has a top plane. The noble metal nano-particle layer is uniformly distributed on the top plane of the planar waveguide. The cover has a notch, and is covered on the top plane of the planar waveguide to form a space between the notch and the top plane. Accordingly, a plurality of incident lights are led into the sensing apparatus with noble metal nano-particle to detect a testing substance contained in the aforesaid space.

摘要翻译： 本发明公开了一种具有贵金属和感测系统的感测装置及其方法。 贵金属的感测装置包括平面波导，贵金属纳米颗粒层和盖。 平面波导具有顶面。 贵金属纳米粒子层均匀地分布在平面波导的顶面上。 盖具有凹口，并且被覆在平面波导的顶平面上，以在凹口和顶平面之间形成空间。 因此，将多个入射光引入到具有贵金属纳米粒子的感测装置中，以检测包含在上述空间中的测试物质。

公开(公告)号：US08349275B2

公开(公告)日：2013-01-08

申请号：US12359080

申请日：2009-01-23

申请人： Shau-Chun Wang ,  Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Chia-Yu Lee

发明人： Shau-Chun Wang ,  Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Chia-Yu Lee

IPC分类号： G01N21/62 ,  G01N33/53

CPC分类号： G01N21/7703 ,  G01N21/554 ,  G01N2021/7736 ,  G01N2021/7783

摘要： A microfluidic device with microstructure includes a channel for accommodating an electrolytic solution therein and at least one microstructure formed in the channel. When an alternating-current signal is input to the microfluidic device so that a surface of the microstructure is polarized by a generated electric field, ions having polarity reverse to that of an electrolytic solution will migrate to the surface of the microstructure to form a field-induced electrical double layer to result in electro-osmotic flows at the corners at two sides of the microstructure, which causes formation of relatively fierce circular vortices in the solution. A sensing system and a sensing method using the microfluidic device with microstructure are also disclosed.

摘要翻译： 具有微结构的微流体装置包括用于容纳其中的电解溶液的通道和在通道中形成的至少一个微结构。 当将交流信号输入到微流体装置以使得微结构的表面被产生的电场极化时，具有与电解液相反极性的极性的离子将迁移到微结构的表面， 导致电双层，导致微观结构两侧角落处的电渗流，这导致在溶液中形成相对激烈的圆形涡流。 还公开了使用具有微结构的微流体装置的感测系统和感测方法。

公开(公告)号：US20070109544A1

公开(公告)日：2007-05-17

申请号：US11558850

申请日：2006-11-10

申请人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu ,  Po-Liang Chen

发明人： Lai-Kwan Chau ,  Wen-Hsin Hsieh ,  Wei-Ting Hsu ,  Po-Liang Chen

IPC分类号： G01N21/55

CPC分类号： G01N21/554 ,  B01L3/5027 ,  G01N21/7703 ,  G01N2021/258

摘要： A surface plasmon resonance sensing system comprised of an incidence illuminant, a modified optical fiber, a layer of gold nanoparticles, a micro-fluidic module, and an illuminant detector; the modified optical fiber related to an optical fiber stripped off a portion of its protection layer and a cladding layer; the layer of noble metal nanoparticles covering over the modified optical fiber; the micro-fluidic module related to a micro-fluidic chip for containing the modified optical fiber and a sample; and the illuminant detector operating to detect the light emitted from the modified optical fiber.

摘要翻译： 一种表面等离子体共振传感系统，包括入射光源，改性光纤，金纳米颗粒层，微流体模块和光源检测器; 与被剥离掉其保护层的一部分的光纤相关的改性光纤和包覆层; 覆盖改性光纤的贵金属纳米粒子层; 微流体模块与用于容纳修改光纤的微流体芯片和样品相关; 并且所述光源检测器操作以检测从所述修改的光纤发射的光。

公开(公告)号：US08662860B2

公开(公告)日：2014-03-04

申请号：US12754092

申请日：2010-04-05

申请人： Wen-Hsin Hsieh ,  Jhih-Lin Chen ,  Wei-Hung Shih

发明人： Wen-Hsin Hsieh ,  Jhih-Lin Chen ,  Wei-Hung Shih

IPC分类号： F04F99/00

CPC分类号： B01J19/0093 ,  B01F13/0071 ,  B01F13/0076 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00972 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2400/0418 ,  F04B19/006

摘要： A microfluidic driving system includes a first planar electrode, a second planar electrode, a third planar electrode, a fourth planar electrode, a power supply unit and a detection module. The second, third and fourth planar electrodes are disposed parallel to the first planar electrode and face-to-face with the first planar electrode to form an accommodation space for accommodating a fluid. An AC power is provided by the power supply unit and an AC electrical field is applied by alternately connecting the third planar electrode and the fourth planar electrode with the first planar electrode for driving the first fluid and the second fluid to flow; and then AC electrical field is also applied by connecting the second planar electrode to the first planar electrode to mix the first fluid and the second fluid. Finally, a detection is performed upon a mixture of the fluids through the detection module.

摘要翻译： 微流体驱动系统包括第一平面电极，第二平面电极，第三平面电极，第四平面电极，电源单元和检测模块。 第二，第三和第四平面电极平行于第一平面电极设置并与第一平面电极面对面地形成用于容纳流体的容置空间。 由电源单元提供AC电力，并且通过交替地将第三平面电极和第四平面电极连接到用于驱动第一流体的第一平面电极和第二流体流动而施加AC电场; 然后通过将第二平面电极连接到第一平面电极来混合第一流体和第二流体来施加AC电场。 最后，通过检测模块对流体的混合物进行检测。