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    Field Effect Transistor For Measuring Biocomponents
    1.
    发明申请
    Field Effect Transistor For Measuring Biocomponents 失效
    用于测量生物组分的场效应晶体管

    公开(公告)号：US20070284630A1

    公开(公告)日：2007-12-13

    申请号：US10591413

    申请日：2005-03-01

    申请人： Werner Baumann ,  Mirko Lehmann ,  Ingo Freund ,  Hans-Jurger Gahle

    发明人： Werner Baumann ,  Mirko Lehmann ,  Ingo Freund ,  Hans-Jurger Gahle

    IPC分类号： H01L29/76 ,  C12Q1/02

    CPC分类号： G01N33/48728 ,  G01N27/4145

    摘要： The invention relates to a device for measuring living cells or similar biocomponents comprising a field effect transistor which is provided with a source, a drain and a channel area placed on a substrate. Said channel area connects said source and drain and is provided with a gate-electrode mounted thereon. The gate electrode has at least two laterally disposed parallel electrode areas which are perpendicular to a direction in which the channel area connects the source to the drain in such a way that they are distant and electrically insulated from each other.

    摘要翻译： 本发明涉及用于测量活细胞或类似生物组件的装置，其包括场效应晶体管，该场效应晶体管设置有放置在基板上的源极，漏极和沟道区域。 所述通道区域连接所述源极和漏极，并且设置有安装在其上的栅电极。 栅电极具有至少两个横向设置的平行电极区域，其垂直于沟道区域将源极连接到漏极的方向，使得它们彼此远离并且电绝缘。

    DEVICE FOR PERFORMING ANALYSIS ON CELL CULTURES
    2.
    发明申请
    DEVICE FOR PERFORMING ANALYSIS ON CELL CULTURES 有权

    公开(公告)号：US20080145922A1

    公开(公告)日：2008-06-19

    申请号：US11952219

    申请日：2007-12-07

    申请人： Mirko Lehmann ,  Martin Brischwein ,  Werner Baumann ,  Ralf Ehret ,  Ingo Freund

    发明人： Mirko Lehmann ,  Martin Brischwein ,  Werner Baumann ,  Ralf Ehret ,  Ingo Freund

    IPC分类号： C12M1/00

    CPC分类号： C12M41/46 ,  C12M27/02 ,  C12M33/04

    摘要： A device (1) for the carrying out of investigations on cell cultures (2) which take place in a liquid culture medium (4). The device possesses at least a receptacle (3) to contain the culture medium (4) including the cell culture (2), wherein one or more sensors (6) for measurement of the cell culture activities is provided and wherein the receptacle (3) has at least an opening for the adding and the removal of liquid culture medium (4) and the like. A separating element (7) can be introduced into the open upper part of the receptacle (3) and can be brought within the said receptacle (3) in a position close to the bottom (5), in which the said separating element (7) makes a boundary forming a partial space (8) which has a small volume in relation to the entire volume of the receptacle (3). Further, a flow channel (9) is provided, which first, communicates with the small volume partial space (8), and second, communicates with the said reservoir (14). This flow channel (9) and/or the sensors (6) are placed in the area of the small volume partial space (8). By means of the capability for adjustment of the separating element (7) as to its distance from the bottom of the receptacle (3), in the neighborhood of the cell culture (2) a microreaction volume exactly fitting the current requirements can be adjusted. By means of the flow channel (9), the cell culture (2) in the small volume partial space (8) stands in fluid communication with that in the reservoir. For the regeneration of the cell culture solution in the small volume partial space, the separating element (7) can be elevated and depressed, or it is possible, to inject into the small volume partial space of the receptacle (3) a quantity of liquid and by means of a through-flow channel. In this case, a convective mixing of fresh and spent medium takes place through the flow channel (9) between the small volume partial space and the reservoir (14).

    Field effect transistor for measuring biocomponents
    4.
    发明授权
    Field effect transistor for measuring biocomponents 失效
    用于测量生物组分的场效应晶体管

    公开(公告)号：US07470962B2

    公开(公告)日：2008-12-30

    申请号：US10591413

    申请日：2005-03-01

    申请人： Werner Baumann ,  Mirko Lehmann ,  Ingo Freund ,  Hans-Jurgen Gahle

    发明人： Werner Baumann ,  Mirko Lehmann ,  Ingo Freund ,  Hans-Jurgen Gahle

    IPC分类号： H01L31/00

    CPC分类号： G01N33/48728 ,  G01N27/4145

    摘要： The invention relates to a device for measuring living cells or similar biocomponents comprising a field effect transistor which is provided with a source, a drain and a channel area placed on a substrate. Said channel area connects said source and drain and is provided with a gate-electrode mounted thereon. The gate electrode has at least two laterally disposed parallel electrode areas which are perpendicular to a direction in which the channel area connects the source to the drain in such a way that they are distant and electrically insulated from each other.

    摘要翻译： 本发明涉及用于测量活细胞或类似生物组件的装置，其包括场效应晶体管，该场效应晶体管设置有放置在基板上的源极，漏极和沟道区域。 所述通道区域连接所述源极和漏极，并且设置有安装在其上的栅电极。 栅电极具有至少两个横向设置的平行电极区域，其垂直于沟道区域将源极连接到漏极的方向，使得它们彼此远离并且电绝缘。

    Device for performing analysis on cell cultures
    5.
    发明授权
    Device for performing analysis on cell cultures 有权
    用于对细胞培养进行分析的装置

    公开(公告)号：US07795012B2

    公开(公告)日：2010-09-14

    申请号：US11952219

    申请日：2007-12-07

    申请人： Mirko Lehmann ,  Martin Brischwein ,  Werner Baumann ,  Ralf Ehret ,  Ingo Freund

    发明人： Mirko Lehmann ,  Martin Brischwein ,  Werner Baumann ,  Ralf Ehret ,  Ingo Freund

    IPC分类号： C12M1/34

    CPC分类号： C12M41/46 ,  C12M27/02 ,  C12M33/04

    摘要： A device for investigating activities of cell cultures in a liquid culture medium includes a receptacle for the culture medium, and one or more sensors for measurement of cell culture activities. An opening for adding and removing liquid culture medium is provided. A separating element can be placed close to the receptacle bottom, forming a partial space with a small volume relative to the entire volume. A flow channel that communicates with the small volume and the reservoir and/or the sensors are placed in the small volume partial space. By adjustment of the separating element distance from the receptacle bottom, a microreaction volume exactly fitting the current requirements is obtained. For regeneration of the cell culture solution in the small volume partial space, the separating element can be elevated and depressed, or liquid can be injected into the small volume by the through-flow channel, providing convective mixing of fresh and spent medium.

    摘要翻译： 用于调查液体培养基中细胞培养物的活性的装置包括用于培养基的容器和用于测量细胞培养活性的一个或多个传感器。 提供了用于添加和除去液体培养基的开口。 分离元件可以靠近容器底部放置，形成相对于整个体积具有小体积的部分空间。 与小容量和储存器和/或传感器连通的流动通道被放置在小体积部分空间中。 通过调节与容器底部的分离元件距离，可以获得与当前要求完全一致的微反应体积。 为了在小体积部分空间中再生细胞培养液，分离元件可以被升高和降低，或者可以通过通流通道将液体注入到小容积中，从而提供新鲜和废弃的介质的对流混合。

    Apparatus for intracellular manipulation of a biological cell
    6.
    发明授权
    Apparatus for intracellular manipulation of a biological cell 失效
    用于细胞内操作生物细胞的装置

    公开(公告)号：US07501276B2

    公开(公告)日：2009-03-10

    申请号：US10202419

    申请日：2002-07-23

    申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    IPC分类号： C12M1/42 ,  C12N15/87

    CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

    摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

    摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封在一起，并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

    Method for intracellular manipulation of a biological cell
    8.
    发明授权
    Method for intracellular manipulation of a biological cell 有权
    细胞内操作生物细胞的方法

    公开(公告)号：US06475760B1

    公开(公告)日：2002-11-05

    申请号：US09321051

    申请日：1999-05-27

    申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

    IPC分类号： C12N1300

    CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

    摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

    摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

    Method for examination of a surface layer
    10.
    发明授权
    Method for examination of a surface layer 失效
    表层检查方法

    公开(公告)号：US06656678B1

    公开(公告)日：2003-12-02

    申请号：US09585146

    申请日：2000-06-01

    申请人： Bernhard Wolf ,  Hans-Jürgen Gahle ,  Günter Igel ,  Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann

    发明人： Bernhard Wolf ,  Hans-Jürgen Gahle ,  Günter Igel ,  Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann

    IPC分类号： C12Q100

    CPC分类号： H01L22/24 ,  G01B11/30 ,  G01N21/94

    摘要： In a method for examination of the surface of an object for a topographic and/or a chemical property, the object-surface is impinged with surface-structure selective biocomponents for examination of a topographic property and/or with chemoselective biocomponents for the examination of a chemical property, together with a nutrient medium and/or an osmotic protective medium for the biocomponents. The biocomponents contained in the nutrient medium and/or the osmotic protective medium are in contact with the object-surface or are spaced from the object surface by less than the detection range of the biocomponents. The object surface is then examined with the biocomponents contained in the nutrient medium and/or the osmotic protective medium by determining at least one examination measurement value. The examination measurement value is compared with a reference measurement value, and conclusions can be drawn about the topographic and/or chemical properties of the object from the result of the comparison. Using the method, an object surface can be examined for a topographic and/or chemical property in a simple manner with a high measuring sensitivity.

    摘要翻译： 在用于检查地形和/或化学性质的物体的表面的方法中，物体表面与表面结构选择性生物组分碰撞以检查地形性质和/或化学选择性生物成分用于检查 化学性质，以及生物组分的营养培养基和/或渗透保护介质。 包含在营养培养基和/或渗透保护介质中的生物成分与物体表面接触或与物体表面间隔小于生物成分的检测范围。 然后通过确定至少一个检查测量值用包含在营养培养基和/或渗透保护介质中的生物组分检查物体表面。 将检查测量值与参考测量值进行比较，并从比较结果中得出关于物体的地形和/或化学性质的结论。 使用该方法，可以以高测量灵敏度的简单方式检查物体表面的地形和/或化学特性。