公开(公告)号：US11851670B2

公开(公告)日：2023-12-26

申请号：US16438424

申请日：2019-06-11

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Bryce Woodbury Carey ,  Yaqub Hanna

IPC: C12N5/074 ,  C12N15/86 ,  G01N33/50 ,  A01K67/027 ,  C07K14/47 ,  C12N15/85 ,  C12N5/0735 ,  C12N15/79

CPC classification number: C12N15/86 ,  A01K67/0271 ,  C07K14/4705 ,  C12N5/0696 ,  G01N33/5008 ,  C12N5/0606 ,  C12N15/79 ,  C12N15/85 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/11 ,  C12N2506/115 ,  C12N2510/00 ,  C12N2740/15041 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2840/203

Abstract: The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to chimeric animals comprising reprogrammed somatic cells of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells.

公开(公告)号：US20230085214A1

公开(公告)日：2023-03-16

申请号：US17869611

申请日：2022-07-20

Applicant: President and Fellows of Harvard College ,  Whitehead Institute for Biomedical Research

Inventor： Hua Wang ,  Andrew Salim Khalil ,  David J. Mooney ,  Rudolf Jaenisch

IPC: A61K47/54 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90

Abstract: Disclosed herein are compositions and methods for labeling cells using click chemistry reagents. The compositions and methods disclosed herein provide a specific and efficient means of localizing desired agents to a variety of cell types in vivo and in vitro. The compositions and methods disclosed herein can be used to deliver a variety of desired agents to a cell or population of cells to direct cell fate and/or cell differentiation.

公开(公告)号：US11434476B2

公开(公告)日：2022-09-06

申请号：US16326700

申请日：2017-08-18

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  X Shawn Liu ,  Hao Wu

IPC: C12N9/22 ,  A61K35/30 ,  A61K35/33 ,  C12N9/10 ,  C12N15/11 ,  C12N15/86 ,  C12N15/90 ,  A61P3/10 ,  A61P9/00 ,  A61P9/10 ,  A61P9/12 ,  A61P11/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28 ,  A61P35/00 ,  A61P37/06 ,  A61K38/46 ,  C12N15/00 ,  C12N15/113 ,  A61K31/7088

Abstract: The invention relates to methods of modifying DNA methylation by contacting a catalytically inactive site specific nuclease fused to an effector domain having methylation or demethylation activity and one or more guide sequences.

公开(公告)号：US20210230538A1

公开(公告)日：2021-07-29

申请号：US17071627

申请日：2020-10-15

Applicant: Whitehead Institute for Biomedical Research ,  Dana-Farber Cancer Institute, Inc.

Inventor： Thorold W. Theunissen ,  Nathanael S. Gray ,  Rudolf Jaenisch

IPC: C12N5/0735 ,  C12N5/074

Abstract: The present disclosure provides compounds of any one of Formulae (A) to (L). The present disclosure also provides compositions, uses, and methods that include or involve a compound described herein, a serine/threonine-protein kinase B-Raf (BRAF) inhibitor, an epidermal growth factor receptor (EGFR) inhibitor, a vascular endothelial growth factor 1 (VEGFR1) inhibitor, a fibroblast growth factor receptor 1 (FGFR1) inhibitor, or a combination thereof. The compounds, compositions, uses, and methods are useful in changing the pluripotency state of a vertebrate cell to a more nave state.

公开(公告)号：US20200239855A1

公开(公告)日：2020-07-30

申请号：US16665821

申请日：2019-10-28

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC: C12N5/074 ,  C12N15/85 ,  C07K14/47 ,  C12N15/877 ,  A01K67/027

Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

公开(公告)号：US20190241874A1

公开(公告)日：2019-08-08

申请号：US16147003

申请日：2018-09-28

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Yaqub Hanna ,  Marius Wernig ,  Christopher J. Lengner ,  Alexander Meissner ,  Oliver Tobias Brambrink ,  G. Grant Welstead ,  Ruth Foreman

IPC: C12N5/074 ,  C07K14/47

CPC classification number: C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2506/11 ,  C12N2506/13 ,  C12N2800/30 ,  C12N2830/003 ,  C12N2830/006 ,  G01N33/5023 ,  G01N2333/91011

Abstract: The disclosure relates to a method of reprogramming one or more somatic cells, e.g., partially differentiated or fully/terminally differentiated somatic cells, to a less differentiated state, e.g., a pluripotent or multipotent state. In further embodiments the invention also relates to reprogrammed somatic cells produced by methods of the invention, to uses of said cells, and to methods for identifying agents useful for reprogramming somatic cells.

公开(公告)号：US20190078057A1

公开(公告)日：2019-03-14

申请号：US16030815

申请日：2018-07-09

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC: C12N5/074 ,  C12N15/85 ,  A01K67/027 ,  C07K14/47 ,  C12N15/877

CPC classification number: C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

公开(公告)号：US20170240865A1

公开(公告)日：2017-08-24

申请号：US15588062

申请日：2017-05-05

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

公开(公告)号：US09169490B2

公开(公告)日：2015-10-27

申请号：US13646430

申请日：2012-10-05

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC: C12N15/85 ,  C12N5/074 ,  A01K67/027 ,  C07K14/47 ,  C12N15/877

CPC classification number: C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.

Abstract translation: 本发明提供了重编程体细胞产生多潜能或多潜能细胞的方法。 这些方法可用于各种目的，包括治疗或预防个体的医疗状况。 本发明进一步提供了用于鉴定将体细胞重新编程为较差分化状态的药剂的方法。

公开(公告)号：US20130109089A1

公开(公告)日：2013-05-02

申请号：US13646411

申请日：2012-10-05

Applicant: Whitehead Institute for Biomedical Research

Inventor： Rudolf Jaenisch ,  Konrad Hochedlinger

IPC: C12N15/85

CPC classification number: C12N5/0696 ,  A01K67/0273 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  C07K14/4702 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/8775 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/605 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2830/003 ,  C12N2830/006

Abstract: The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state.