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40 条记录 (耗时 0.031 秒)

    Design of novel highly efficient HIV based packaging systems for gene therapy
    5.
    发明授权
    Design of novel highly efficient HIV based packaging systems for gene therapy 失效
    用于基因治疗的新颖高效的基于HIV的包装系统的设计

    公开(公告)号：US06323019B1

    公开(公告)日：2001-11-27

    申请号：US09131684

    申请日：1998-08-10

    申请人： Pierre Corbeau ,  Gunter Kraus ,  Flossie Wong-Staal

    发明人： Pierre Corbeau ,  Gunter Kraus ,  Flossie Wong-Staal

    IPC分类号： C12N1564

    CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16052 ,  C12N2740/16122

    摘要： By transducing cells with an HIV-1-MN molecular clone deleted in the major packaging sequence, a stable HIV-1 packaging cell line, &psgr;422 was produced. &psgr;422 cells form syncytia with CD4 positive cells, correctly express HIV-1 structural proteins, and produce large amount of mature particles with normal RT activity. These particles are not infectious. When stably transfected with an HIV-based retroviral vector, the &psgr;422 cell line produces hybrid virions capable of transducing CD4 positive cells with high efficiency (e.g., 105 cells/ml). The availability of this stable, noninfectious HIV-1 packaging cell line capable of generating high titer HIV vectors enables the use of HIV-1 based nucleic acids delivery systems, for example, in gene therapy. An HIV-2 based vector is packaged by the packaging cell lines, demonstrating that HIV-2 cell transformation vectors are packaged by the packaging cell line. HIV based vectors packaged by the high efficiency cell lines are shown to have anti-HIV activity per se.

    摘要翻译： 通过在主要包装序列中缺失的HIV-1-MN分子克隆转导细胞，产生稳定的HIV-1包装细胞系psi422。 psi422细胞与CD4阳性细胞形成合胞体，正确表达HIV-1结构蛋白，并产生大量具有正常RT活性的成熟颗粒。 这些颗粒不感染。 当用HIV-型逆转录病毒载体稳定转染时，psi422细胞系产生能够以高效率（例如，105个细胞/ ml）转导CD4阳性细胞的杂合病毒体。 能够产生高滴度的HIV载体的这种稳定的，非感染性的HIV-1包装细胞系的可用性使得能够使用基于HIV-1的核酸递送系统，例如在基因治疗中。 通过包装细胞系包装基于HIV-2的载体，证明HIV-2细胞转化载体被包装细胞系包装。 由高效细胞系包装的基于HIV的载体显示出具有抗HIV活性本身。

    Isolation and purification of the eighth gene of HTLV-III
    10.
    发明授权
    Isolation and purification of the eighth gene of HTLV-III 失效
    分离和纯化HTLV-III的第八个基因

    公开(公告)号：US4963497A

    公开(公告)日：1990-10-16

    申请号：US920780

    申请日：1986-10-20

    申请人： Flossie Wong-Staal ,  Pranab K. Chanda ,  John Ghrayeb

    发明人： Flossie Wong-Staal ,  Pranab K. Chanda ,  John Ghrayeb

    IPC分类号： A61K38/00 ,  C07K14/16 ,  C07K16/10 ,  C12N15/48

    CPC分类号： C07K14/005 ,  C07K16/10 ,  A61K38/00 ,  C12N2740/16322

    摘要： The present invention is the isolation and purification of a newly discovered gene of the AIDS virus, HTLV-III, which encodes a protein which is immunogenic and recognized by sera of some HTLV-III seropositive people. Furthermore, the gene is highly conserved among all known HTLV-III isolates and exhibits a polymorphism at the 3' end which distinguishes molecular clones of the HTLV-III cell line from viral genomes of related viruses (i.e., other HTLV-III isolates, LAV, ARV, etc.).

    摘要翻译： 本发明是新发现的艾滋病病毒基因HTLV-III的分离和纯化，HTLV-III编码免疫原性并被一些HTLV-III血清阳性人血清识别的蛋白质。 此外，该基因在所有已知的HTLV-III分离物中是高度保守的，并且在3'末端显示多态性，其区分HTLV-III细胞系的分子克隆与相关病毒的病毒基因组（即，其他HTLV-III分离株，LAV ，ARV等）。