Vaccine delivery system
    2.
    发明授权
    Vaccine delivery system 失效
    疫苗输送系统

    公开(公告)号:US4022206A

    公开(公告)日:1977-05-10

    申请号:US600983

    申请日:1975-08-01

    IPC分类号: A61M5/28 A61M5/00

    CPC分类号: A61M5/282 A61M5/288

    摘要: Vaccine delivery system having a lyophilized vaccine in human dosage concentration loaded into presterilized single dosage syringe having a squeezable body portion and a separate package containing enough water for injection to reconstitute the single dose of lyophilized vaccine.

    摘要翻译: 将具有人体剂量浓度的冻干疫苗的疫苗递送系统装载到具有可挤压主体部分的预杀灭的单剂量注射器中,以及包含足够的注射用水的单独包装以重构单剂量的冻干疫苗。

    Syringe containing frozen vaccine
    3.
    发明授权
    Syringe containing frozen vaccine 失效
    含冷冻疫苗的注射器

    公开(公告)号:US4018222A

    公开(公告)日:1977-04-19

    申请号:US577244

    申请日:1975-05-13

    IPC分类号: A61M5/28 A61M5/00

    CPC分类号: A61M5/282 A61M5/288

    摘要: Vaccine delivery system wherein an aqueous vaccine diluted to human dosage concentration is loaded into presterilized single dosage syringes having a squeezable body portion and wherein a plurality of these syringes are sealed inside a bag and the contents of the syringes are frozen to preserve the titer of the vaccine.

    摘要翻译: 疫苗递送系统,其中将稀释至人的剂量浓度的水性疫苗装载到具有可挤压主体部分的预灭灭的单剂量注射器中,并且其中将多个这些注射器密封在袋内,并将注射器的内容物冷冻以保持 疫苗。

    Method for recovering embryos from embryonated eggs
    4.
    发明授权
    Method for recovering embryos from embryonated eggs 失效
    从胚胎卵中回收胚胎的方法

    公开(公告)号:US4187989A

    公开(公告)日:1980-02-12

    申请号:US917811

    申请日:1978-06-22

    IPC分类号: A01K43/00 B01D35/20 B02C19/18

    CPC分类号: A01K43/00 B01D35/20

    摘要: A method and apparatus for recovering embryos from embryonated eggs wherein the egg is cracked open the shell discarded and the contents dropped onto a continuously vibrating screen which separates the embryo from the yolk sac and accompanying fluid. This associated material, which is less cohesive than the embryo, passes through the screen. The screen itself is tilted at a compound angle from the horizontal and its vibrating action causes the embryos to move along the upper surface of the screen toward a discharge chute located at the lower corner of the screen.

    摘要翻译: 一种用于从胚胎卵中回收胚胎的方法和装置,其中蛋破裂,将所丢弃的壳开放并将内容物滴落到将卵与卵黄囊和伴随液体分离的连续振动筛上。 这种相对于胚胎粘合力较小的材料通过筛网。 屏幕本身以与水平面的复合角度倾斜,并且其振动作用使胚胎沿着屏幕的上表面朝向位于屏幕下角的排放槽移动。

    Apparatus for preparing isolated cells from tissue
    5.
    发明授权
    Apparatus for preparing isolated cells from tissue 失效
    用于从组织制备分离的细胞的装置

    公开(公告)号:US4028190A

    公开(公告)日:1977-06-07

    申请号:US632610

    申请日:1975-11-17

    CPC分类号: B04B1/04 C12M45/02 C12M45/09

    摘要: An apparatus and a method for preparing live, sterile cells from tissue are disclosed which enable high yields of viable isolated cells to be obtained with a greatly reduced possibility of contamination of the cells during the process. The apparatus and method allows one to truly mass manufacture isolated cells as contrasted to a small hand operation requiring many hours and dozens of people. The improved apparatus of the invention comprises a shredder device which mechanically shreds the tissue into small pieces of high surface to volume ratio, a processing vessel in which the shredded tissues are contacted with an enzyme solution to break down the intercellular material and thereby separate the cells one from the other and a means for separating the cells from the enzyme solution. Preferably, the means for separating the cells from the enzyme solution is a flow centrifuge which has been specifically designed to reduce shear force inside the bowl during operation. Also disclosed is a process for shredding tissue employing a plurality of moveable blade elements and at least a corresponding number of separated fixed elements. Interposing the tissue between the fixed and rotating elements and then driving the moveable blade elements through the spaces defined by adjacent fixed elements results in the tissue being shredded into small pieces of high surface to volume ratio. This in turn optimizes surface area for contact between the shredded tissue and the enzyme solution. In addition, a completely closed process is disclosed for obtaining cells from the tissue comprising the steps of sequentially shredding the tissue, contacting the shredded tissue at a controlled temperature with an enzyme solution to break down the intercellular material and then separating the isolated cells from the enzyme solution.

    摘要翻译: 公开了一种用于从组织制备活的无菌细胞的装置和方法,其使得可以在该过程中大大降低细胞污染的可能性而获得高得多的活的分离的细胞。 该装置和方法允许真正大量制造隔离的细胞,与需要数十小时和数十人的小手操作相反。 本发明的改进的装置包括一个粉碎装置,其将组织机械地切碎成高的表面与体积比的小块;处理容器,其中切碎的组织与酶溶液接触以分解细胞间材料,从而分离细胞 一个来自另一个和用于从酶溶液中分离细胞的方法。 优选地,用于从酶溶液中分离细胞的装置是流动离心机,其已经被专门设计用于在操作期间减小碗内的剪切力。 还公开了一种使用多个可移动叶片元件和至少相应数量的分离的固定元件来切碎组织的方法。 将组织插入固定和旋转元件之间,然后通过由相邻的固定元件限定的空间驱动可移动的刀片元件,导致组织被切碎成高的表面与体积比的小块。 这又优化了切碎的组织和酶溶液之间的接触的表面积。 另外,公开了一种从组织获得细胞的完全封闭的方法,包括以下步骤:顺序地切碎组织,在受控温度下将切碎的组织与酶溶液接触以分解细胞间材料,然后将分离的细胞从 酶溶液。

    Assay for hepatitis A antigen
    8.
    发明授权
    Assay for hepatitis A antigen 失效
    甲型肝炎抗原测定

    公开(公告)号:US4293538A

    公开(公告)日:1981-10-06

    申请号:US31712

    申请日:1979-04-20

    摘要: Liquid containing hepatitis A antibody is adsorbed on a surface. The surface is then coated with a proteinaceous material and incubated in the presence of the sample to form a complex of hepatitis A antigen and hepatitis A antibody. The complex is incubated again in the presence of excess radioactively labelled hepatitis A antibody, and the resulting radionuclide is measured.

    摘要翻译: 含有甲型肝炎抗体的液体被吸附在表面上。 然后将表面用蛋白质材料包被,并在样品存在下孵育以形成甲型肝炎抗原和甲型肝炎抗体的复合物。 在过量放射性标记的甲型肝炎抗体存在下再次培养该复合物,并测量所得的放射性核素。

    Automated virus harvesting
    9.
    发明授权
    Automated virus harvesting 失效
    自动病毒收集

    公开(公告)号:US4242457A

    公开(公告)日:1980-12-30

    申请号:US13494

    申请日:1979-02-21

    IPC分类号: C12M3/00 C12N7/00

    摘要: Automated means are provided to harvest virus at a time when the yield is optimized and store the harvest with minimum loss thereby eliminating difficulties when the optimum yield time does not fall within normal working hours, enhancing sterility by limiting human contact and enhancing stability of the product.

    摘要翻译: 提供自动装置以在产量优化的时候收获病毒,并以最小的损失存储收获,从而在最佳产量时间不在正常工作时间内消除困难,通过限制人的接触和增强产品的稳定性来增强不育 。

    Assay for hepatitis a virus
    10.
    发明授权
    Assay for hepatitis a virus 失效
    甲型肝炎病毒检测

    公开(公告)号:US4861706A

    公开(公告)日:1989-08-29

    申请号:US857695

    申请日:1986-04-29

    IPC分类号: A61K39/29 C12Q1/70

    CPC分类号: C12Q1/706 Y10S435/948

    摘要: A quantitative assay for HAV comprises infecting at a temperature of from about 30.degree. to about 37.degree. C. a cell sheet with a dilution series of hepatitis A virus and then adding Newcastle disease virus (NDV) to the infected cell sheet and incubating the infected cell sheet in the presence of NDV at an elevated temperature for a time sufficient to permit a cytopathic effect (CPE) to become manifest, this time typically being at least about 4 days and preferably about 5 days. At an incubation temperature of from about 31.degree. to about 33.degree. C. the absence of CPE indicates the presence of HAV and the presence of CPE indicates the absence of HAV. At an incubation temperature of from about 34.degree. to about 36.degree. C. the absence of CPE indicates the absence of HAV and the presence of CPE indicates the presence of HAV.

    摘要翻译: 用于HAV的定量测定包括在约30℃至约37℃的温度下感染具有稀释系列的甲型肝炎病毒的细胞片,然后向感染的细胞片中加入新城疫病毒(NDV)并孵育感染的 细胞片在NDV存在下在升高的温度下持续足以允许细胞病变效应(CPE)变得明显的时间,此时通常为至少约4天,优选约5天。 在约31℃至约33℃的温育温度下,不存在CPE表明存在HAV,而CPE的存在表明不存在HAV。 在约34℃至约36℃的培养温度下,不存在CPE表明不存在HAV,且CPE的存在表明存在HAV。