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公开(公告)号：US5712142A
公开(公告)日：1998-01-27
申请号：US604913
申请日：1996-02-22
CPC分类号： C12P7/10 , C12N9/2437 , C12Y302/01004 , C12Y302/01021 , C07K2319/02 , Y02E50/16
摘要： The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.
摘要翻译： 编码酸热解纤维素分解酶E1内切葡聚糖酶的基因在巴斯德毕赤酵母中克隆并表达。 包含全尺寸E1酶的催化结构域的新的修饰的E1内切葡聚糖酶表现出增强的热稳定性，并通过两种方法产生。 生产新的修饰的E1的第一种方法是蛋白水解切割以去除全尺寸E1的纤维素结合结构域和接头肽。 产生新修饰的E1的第二种方法是编码全长E1的基因的遗传截短，使得催化结构域在表达产物中表达。
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2.发明授权Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂公开(公告)号：US08283150B2
公开(公告)日：2012-10-09
申请号：US12247594
申请日：2008-10-08
申请人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
发明人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.
摘要翻译： 与其他Cel7家族成员酶相比，来自青霉菌（Penicillium funiculosum）的纯化纤维二糖水解酶I（糖基水解酶家族7（Cel7A））酶表现出高水平的特异性，当用来自解纤维解卷菌的纯化的EIcd内切葡聚糖酶配制并在预处理的玉米秸秆上进行测试时。 纯化的天然酶以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶的结果是真实的。 在具体实例中，与使用来自里氏木霉的纯化Cel7A的制剂相比，使用在泡盛曲霉中表达的来自青霉菌的纯化重组Cel7A的制剂的具体表现增加了超过200％。
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3.发明授权Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂公开(公告)号：US07449550B2
公开(公告)日：2008-11-11
申请号：US10557589
申请日：2003-02-27
申请人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
发明人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
IPC分类号： C07K14/00
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.
摘要翻译： 与其他Cel7家族成员酶相比，纯化的纤维二糖水解酶I（糖基水解酶家族7（Cel7A））来自A. cellulolyticus的纯化EIcd内切葡聚糖酶，并与预处理的玉米秸秆进行了测试，结果表明： 纯化的天然酶，以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶。在一个具体实例中，使用在A.A中表达的来自Penicillium funiculosum的纯化重组Cel7A的制剂的具体性能。 与使用来自里氏木霉的纯化Cel7A的制剂相比，泡盛曲增加超过200％。
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4.发明申请Superactive Cellulase Formulation Using Cellobiohydrolase-1 from Penicillium Funiculosum 有权超临界纤维素酶配方使用来自青霉菌的纤维二糖水解酶-1公开(公告)号：US20090081762A1
公开(公告)日：2009-03-26
申请号：US12247594
申请日：2008-10-08
申请人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
发明人： William S. Adney , John O. Baker , Stephen R. Decker , Yat-Chen Chou , Michael E. Himmel , Shi-You Ding
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.
摘要翻译： 与其他Cel7家族成员酶相比，来自青霉菌（Penicillium funiculosum）的纯化纤维二糖水解酶I（糖基水解酶家族7（Cel7A））酶表现出高水平的特异性，当用来自解纤维解卷菌的纯化的EIcd内切葡聚糖酶配制并在预处理的玉米秸秆上进行测试时。 纯化的天然酶以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶的结果是真实的。 在具体实例中，与使用来自里氏木霉的纯化Cel7A的制剂相比，使用在泡盛曲霉中表达的来自青霉菌的纯化重组Cel7A的制剂的具体表现增加了超过200％。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US08465961B2
公开(公告)日：2013-06-18
申请号：US13540641
申请日：2012-07-03
申请人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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7.发明授权
公开(公告)号：US5843760A
公开(公告)日：1998-12-01
申请号：US851767
申请日：1997-05-06
摘要： This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.
摘要翻译： 本发明涉及通常不发酵戊糖的单一微生物，其经遗传改变以发酵戊糖，木糖和阿拉伯糖以产生乙醇，以及利用该微生物的发酵方法。 实例包括用木糖异构酶的大肠杆菌基因，木酮糖激酶，L-阿拉伯糖异构酶，L-核糖激酶，L-核酮糖-5-磷酸4-差向异构酶，转醛醇酶和转酮酶的组合转化的运动发酵单胞菌。 添加基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素或淀粉的水解产生的葡萄糖，木糖和阿拉伯糖，以产生乙醇。
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公开(公告)号：US07998722B2
公开(公告)日：2011-08-16
申请号：US12410501
申请日：2009-03-25
申请人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Luan Tao , Yuying Zhang , Perry G. Caimi , Carol M. McCutchen , Laura McCole , Min Zhang , Yat-Chen Chou , Mary Ann Franden
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译： 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达，并且当该菌株另外被设计用于表达木酮糖激酶，转醛醇酶和转酮酶时，获得木糖的改善的利用。
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9.发明授权Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production 有权用于乙醇生产的木糖利用酵母单体的木糖醇合成突变体公开(公告)号：US07741119B2
公开(公告)日：2010-06-22
申请号：US11862566
申请日：2007-09-27
申请人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
发明人： Paul V. Viitanen , Yat-Chen Chou , Carol M. McCutchen , Min Zhang
摘要： A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
摘要翻译： 用葡萄糖 - 果糖氧化还原酶基因的遗传修饰工程改造了利用木糖的酵母菌菌株，导致GFOR酶活性的降低。 工程化的菌株表现出木糖代谢产生不良副产物木糖醇的减少。 在过程相关条件下，在混合糖发酵过程中，它还消耗更多的木糖并产生更多的乙醇。
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公开(公告)号：US07354755B2
公开(公告)日：2008-04-08
申请号：US09565233
申请日：2000-05-01
申请人： Min Zhang , Yat-Chen Chou
发明人： Min Zhang , Yat-Chen Chou
CPC分类号： C12N9/1205 , C12N9/1022 , C12N9/92 , C12N15/74 , C12N15/90 , C12N15/902 , C12P7/06 , C12P7/065 , Y02E50/17
摘要： The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transformed, is also provided.The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.
摘要翻译： 本发明简要地包括用于将外来基因稳定插入到细菌基因组中的转座子，其包含至少一个操纵子，所述操纵子具有编码选自由二甲酰基A， 细菌中的结构基因，一对倒置插入序列，插入序列内部的操纵子和位于插入序列外部的转座酶基因。 一种用于将外来基因转化到细菌基因组中的质粒穿梭载体，其包含至少一个操纵子，所述操纵子具有编码选自下组的结构基因：xylAxylB，araBAD和tal / tkt，所述启动子用于在 并且还提供了与待转化的细菌基因组中的基因具有同源性的至少两个DNA片段。 根据本发明，转座子和穿梭载体可用于构建显着不同的运动发酵单胞菌菌株，其可用于使用传统发酵技术将纤维素衍生的戊糖转化为燃料和化学品，因为它们对于在 非选择介质。
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