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    Porous membrane device that promotes the differentiation of monocytes into dendritic cells
    9.
    发明申请
    Porous membrane device that promotes the differentiation of monocytes into dendritic cells 审中-公开
    促进单核细胞分化成树突状细胞的多孔膜装置

    公开(公告)号：US20070178076A1

    公开(公告)日：2007-08-02

    申请号：US11642926

    申请日：2006-12-21

    申请人： Donald Drake ,  David Moe ,  Conan Li ,  Heather Fahlenkamp ,  Guzman Sanchez-Schmitz ,  Russell Higbee ,  Robert Parkhill ,  William Warren

    发明人： Donald Drake ,  David Moe ,  Conan Li ,  Heather Fahlenkamp ,  Guzman Sanchez-Schmitz ,  Russell Higbee ,  Robert Parkhill ,  William Warren

    IPC分类号： A61K48/00 ,  C12M3/00 ,  C12N5/08

    CPC分类号： C12N5/0639 ,  C12M23/12 ,  C12M23/20 ,  C12M25/02 ,  C12M25/14 ,  C12N2502/28 ,  C12N2503/00

    摘要： Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other in vitro-generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses.

    摘要翻译： 用于研究和临床应用的树突状细胞（DC）通常衍生自在细胞因子混合物中培养一周或更长时间的纯化血液单核细胞。 由于已经提出这些细胞因子衍生的DC可能在一些重要的免疫功能上有缺陷，并且可能不能准确地代表在生理条件下发现的抗原呈递细胞（APC）群体，所以需要一种允许在 更生理相关的方式。 本发明包括一种用于产生大量高度纯化的DC的简单可靠的技术，其基于通过在例如Transwell装置中培养的内皮细胞的血液单核细胞的单次迁移。 从较低的Transwell室收获的所得APC在其主要组织相容性（MHC）和共刺激分子的表达，吞噬外来抗原的能力和触发抗原特异性T细胞的能力方面类似于其它体外产生的DC群体 回应。