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公开(公告)号：US08058033B2
公开(公告)日：2011-11-15
申请号：US12292563
申请日：2008-11-20
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20120149087A1
公开(公告)日：2012-06-14
申请号：US13251116
申请日：2011-09-30
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08551755B2
公开(公告)日：2013-10-08
申请号：US12680193
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
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公开(公告)号：US08679792B2
公开(公告)日：2014-03-25
申请号：US13251116
申请日：2011-09-30
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US20110014681A1
公开(公告)日：2011-01-20
申请号：US12680193
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本发明涉及葡糖淀粉酶变体。 特别地，本发明涉及葡糖淀粉酶的淀粉结合结构域（SBD）中的变体。 本发明还涉及与相应的母体葡糖淀粉酶相比具有改变的性质（例如，改善的热稳定性和/或增加的比活性）的变体。 本发明还提供了包含变体葡糖淀粉酶的酶组合物; 包含编码变体的多核苷酸的DNA构建体; 以及在宿主细胞中产生葡糖淀粉酶变体的方法。
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公开(公告)号：US08592194B2
公开(公告)日：2013-11-26
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法。
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公开(公告)号：US20110020899A1
公开(公告)日：2011-01-27
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells
摘要翻译： 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法
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公开(公告)号：US20090275080A1
公开(公告)日：2009-11-05
申请号：US12292563
申请日：2008-11-20
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译： 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。
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公开(公告)号：US08916369B2
公开(公告)日：2014-12-23
申请号：US12531970
申请日：2008-03-12
CPC分类号： C12N9/242
摘要： A maltogenic a-amylase from Trichoderma reesei (TrAA) and variants thereof are useful in the production of high-maltose syrups from liquefied starch. Particularly high maltose concentrations are achieved when TrAA is used in the presence of a pullulanase. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.
摘要翻译： 来自里氏木霉（TrAA）的麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高麦芽糖浆。 当在存在支链淀粉酶的情况下使用TrAA时，可实现特别高的麦芽糖浓度。 还提供了用于产生TrAA及其变体的表达宿主和编码核酸。
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公开(公告)号：US20190002854A1
公开(公告)日：2019-01-03
申请号：US16064577
申请日：2016-12-20
申请人： Bart C. KOOPS , Paula Johanna Maria TEUNISSEN , Marco VAN BRUSSEL-ZWIJNEN , Martijn SCHEFFERS , Kees-Jan GUIJT , Zhengzheng ZOU , Zhongmei TANG , Zhen QIAN , Jing GE , Zhenghong ZHANG , Danisco US Inc.
发明人： Bart C. Koops , Paula Johanna Maria Teunissen , Marco Van Brussel-Zwijnen , Martijn Scheffers , Kees-Jan Guijt , Zhengzheng Zou , Zhongmei Tang , Zhen Qian , Jing Ge , Zhenghong Zhang
摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.
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