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    Trichoderma reesei α-amylase enhances saccharification of corn starch
    3.
    发明授权
    Trichoderma reesei α-amylase enhances saccharification of corn starch 失效
    里氏木霉α-淀粉酶增强玉米淀粉的糖化

    公开(公告)号：US08318157B2

    公开(公告)日：2012-11-27

    申请号：US12532083

    申请日：2008-03-12

    申请人： Gang Duan ,  Kathy Qian ,  Martijn Scheffers ,  Jayarama K. Shetty ,  Pieter Van Solingen

    发明人： Gang Duan ,  Kathy Qian ,  Martijn Scheffers ,  Jayarama K. Shetty ,  Pieter Van Solingen

    IPC分类号： A61K38/46 ,  C12N9/28

    CPC分类号： C12N9/242

    摘要： A maltogenic α-amylase from Trichoderma reesei (TrAA) and variants thereof in the presence of a glucoamylase are useful in the production of high-glucose syrups from liquefied starch, where the high-glucose syrups produced thereby contain at least about 97% glucose. In this process, TrAA advantageously suppresses the reversion of glucose to malto-oligosaccharides. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.

    摘要翻译： 在葡糖淀粉酶存在下，来自里氏木霉（TrAA）的麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高葡萄糖浆，其中由此产生的高葡萄糖浆含有至少约97％的葡萄糖。 在此过程中，TrAA有利地抑制了葡萄糖向麦芽寡糖的转化。 还提供了用于产生TrAA及其变体的表达宿主和编码核酸。

    Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same
    5.
    发明申请
    Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same 有权
    含有预生物异构异构寡糖的谷物组合物及其制备和使用方法

    公开(公告)号：US20120135108A1

    公开(公告)日：2012-05-31

    申请号：US13172744

    申请日：2011-06-29

    申请人： Gang Duan ,  Feng Li ,  Jayarama K. Shetty ,  Julius Vadakoot ,  Sridevi Vadakoot

    发明人： Gang Duan ,  Feng Li ,  Jayarama K. Shetty ,  Julius Vadakoot ,  Sridevi Vadakoot

    IPC分类号： A23L1/186 ,  A23L1/09

    CPC分类号： A21D2/181 ,  A23L29/35 ,  C07H3/06 ,  C12C5/00 ,  C12P19/04 ,  C12P19/14 ,  C12P19/18 ,  C12P19/22

    摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

    摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使所述麦芽糖与转葡糖苷酶接触，其中所述步骤（a）和步骤（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。

    Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same
    7.
    发明授权
    Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权
    含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法

    公开(公告)号：US07993689B2

    公开(公告)日：2011-08-09

    申请号：US12616595

    申请日：2009-11-11

    申请人： Gang Duan ,  Fengi Li ,  Jayarama K. Shetty ,  Julius Vadakoot ,  Sridevi Vadakoot, legal representative

    发明人： Gang Duan ,  Fengi Li ,  Jayarama K. Shetty ,  Julius Vadakoot

    IPC分类号： A21D2/00 ,  C12P19/14

    CPC分类号： A21D2/181 ,  A23L29/35 ,  C07H3/06 ,  C12C5/00 ,  C12P19/04 ,  C12P19/14 ,  C12P19/18 ,  C12P19/22

    摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

    摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使麦芽糖与转葡糖苷酶接触，其中步骤（a）和（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。