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1.发明授权Bioproduction of astaxanthin using mutant carotenoid ketolase and carotenoid hydroxylase genes 失效使用突变类胡萝卜素酮醇酶和类胡萝卜素羟化酶基因生物生产虾青素公开(公告)号：US07074604B1
公开(公告)日：2006-07-11
申请号：US11025177
申请日：2004-12-29
申请人： Xiao-Song Tang , Qiong Cheng , Joanne Y. Shyr , Luan Tao
发明人： Xiao-Song Tang , Qiong Cheng , Joanne Y. Shyr , Luan Tao
CPC分类号： C12N9/0069 , C12N9/0071 , C12P23/00 , Y10S435/858
摘要： Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.
摘要翻译： 提供编码CrtO酮醇酶和CrtZ羟化酶的蛋白质工程化的核酸片段具有增加的虾青素合成活性。 还提供了使用本发明的核酸片段的方法，用于增加或改变合适的生产宿主中的虾青素生产。
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公开(公告)号：US07425625B2
公开(公告)日：2008-09-16
申请号：US11147915
申请日：2005-06-08
申请人： Xiao-Song Tang , Qiong Cheng , Luan Tao , Joanne Y. Shyr
发明人： Xiao-Song Tang , Qiong Cheng , Luan Tao , Joanne Y. Shyr
CPC分类号： C12P23/00 , C07H21/04 , C12N9/1022
摘要： Protein engineered CrtO ketolases isolated from Rhodococcus erythropolis AN12 are provided having increased carotenoid ketolase activity. Methods using the present CrtO ketolases are also provided for increasing ketocarotenoid production in suitable production hosts.
摘要翻译： 提供从红球菌红细胞生成素AN12分离的蛋白质工程化的CrtO酮醇酶具有增加的类胡萝卜酮酮醇酶活性。 还提供了使用本发明的CrtO酮糖酶的方法，用于在合适的生产主体中增加酮类胡萝卜素生产。
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公开(公告)号：US20060003403A1
公开(公告)日：2006-01-05
申请号：US11147915
申请日：2005-06-08
申请人： Xiao-Song Tang , Qiong Cheng , Luan Tao , Joanne Shyr
发明人： Xiao-Song Tang , Qiong Cheng , Luan Tao , Joanne Shyr
CPC分类号： C12P23/00 , C07H21/04 , C12N9/1022
摘要： Protein engineered CrtO ketolases are provided having increased carotenoid ketolase activity. Methods using the present CrtO ketolases are also provided for increasing ketocarotenoid production in suitable production hosts.
摘要翻译： 提供了具有增加的类胡萝卜酮酮醇酶活性的蛋白质工程化的CrtO酮醇酶。 还提供了使用本发明的CrtO酮糖酶的方法，用于在合适的生产主体中增加酮类胡萝卜素生产。
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4.发明申请公开(公告)号：US20060141558A1
公开(公告)日：2006-06-29
申请号：US11025177
申请日：2004-12-29
申请人： Xiao-Song Tang , Qiong Cheng , Joanne Shyr , Luan Tao
发明人： Xiao-Song Tang , Qiong Cheng , Joanne Shyr , Luan Tao
CPC分类号： C12N9/0069 , C12N9/0071 , C12P23/00 , Y10S435/858
摘要： Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts.
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公开(公告)号：US20110021768A1
公开(公告)日：2011-01-27
申请号：US12857130
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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公开(公告)号：US07985849B2
公开(公告)日：2011-07-26
申请号：US12051255
申请日：2008-03-19
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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7.发明授权Methods for the production of tyrosine, cinnamic acid and para-hydroxycinnamic acid using recombinant microorganisms 失效使用重组微生物生产酪氨酸，肉桂酸和对羟基肉桂酸的方法公开(公告)号：US07303900B2
公开(公告)日：2007-12-04
申请号：US11486826
申请日：2006-07-14
CPC分类号： C12N9/88 , C12P7/40 , C12P7/42 , C12P13/225
摘要： Genes encoding phenylalanine ammonia-lyase (PAL), tyrosine ammonia lyase (TAL) and phenylalanine hydroxylase (PAH) have been introduced into a host organism for the production of Para-hydroxycinnamic acid (PHCA). The introduction of these genes results in the redirection of carbon flow in the host, optimizing the flow of carbon from glucose to PHCA. The intermediates, tyrosine and cinnamic acid are also produced.
摘要翻译： 已经将编码苯丙氨酸氨裂解酶（PAL），酪氨酸氨裂解酶（TAL）和苯丙氨酸羟化酶（PAH）的基因导入宿主生物体中以生产对羟基肉桂酸（PHCA）。 这些基因的引入导致宿主中碳流的重定向，优化碳从葡萄糖到PHCA的流动。 还生产中间体，酪氨酸和肉桂酸。
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公开(公告)号：US06350573B1
公开(公告)日：2002-02-26
申请号：US09304711
申请日：1999-05-04
IPC分类号： C12Q100
CPC分类号： C12Q1/37 , G01N2430/20 , G01N2500/00
摘要： D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
摘要翻译： 已经从藻类（Scenedesmjus obliquus），小麦和集胞藻PCC 6803中分离出D1蛋白酶，并且已经克隆并测序了编码这些酶的基因。 已经使用天然或重组产生的酶开发测定以检测能够抑制D1蛋白酶的除草组合物。
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公开(公告)号：US08445659B2
公开(公告)日：2013-05-21
申请号：US12857120
申请日：2010-08-16
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
摘要翻译： 提出了具有改善的反应动力学的B12依赖性脱水酶的序列。 使用这些B12依赖性脱水酶可以降低甘油和1,3-丙二醇存在下酶自杀灭活的速度。 使用易错PCR和寡核苷酸定向诱变产生酶，以靶向编码甘油脱水酶的α-亚基的DhaB1基因。 使用高通量测定快速鉴定具有改善的反应动力学的突变体。
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公开(公告)号：US20110021767A1
公开(公告)日：2011-01-27
申请号：US12857120
申请日：2010-08-16
IPC分类号： C07H21/04
摘要： Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
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