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公开(公告)号：US07803579B2
公开(公告)日：2010-09-28
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of the sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of the sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac′), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between the sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 该方法包括提供一种引物，其在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）和5'侧杂交的序列（Ac'） 序列（Ac'）与位于靶核酸序列上的序列（A）的5'侧的序列（B）的互补序列（Bc）杂交的序列（B'），其中{X（ Y-Y'）} / X在-1.00至1.00的范围内，其中X表示序列中的碱基数（Ac'），Y表示侧翼为序列（A）的区域中的碱基数， 和（B），Y'表示序列（Ac'）和（B'）之间的间隔序列中的碱基数（Y'可以为零）。
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公开(公告)号：US20060160084A1
公开(公告)日：2006-07-20
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of said sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of said sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac′), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between said sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 在本发明的方法中，引物在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）杂交的序列（Ac'）和5 所述序列（Ac'）的序列（B'）与位于靶核酸序列上的所述序列（A）的5'侧的序列（B）的互补序列（Bc）杂交， 其中{X-（Y-Y'）} / X在-1.00至1.00的范围内，其中X表示所述序列中的碱基数（Ac'），Y表示侧翼的区域中的碱基数 所述序列（A）和（B）在靶核酸序列中，Y'表示所述序列（Ac'）和（B'）之间的间插序列中的碱基数（Y'可以为零）。
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公开(公告)号：US20100024362A1
公开(公告)日：2010-02-04
申请号：US12462152
申请日：2009-07-30
CPC分类号： B65B43/14 , B65B43/18 , B65H1/22 , B65H3/04 , B65H5/10 , B65H2220/09 , B65H2406/341 , B65H2701/191
摘要： An empty bag supply method and apparatus first lifting up with a first suction member an empty bag of which bag bottom being in contact with and positioned by a first positioning stopper. The lifted bag is then held by chucks and transported upwards so that the empty bag is vertical with the bag bottom facing upward, and then a second suction member suctions the empty bag near the bag mouth and transports it onto a conveyor with the bag mouth facing forward, so that the bag mouth of the bag is stopped and positioned by a second positioning stopper. The empty bag is then lifted up by a third suction member, held by a chuck and transported upward so that the attitude of the bag is changed to vertical with the bag mouth facing upward, and then the bag is transferred to a gripper of a packaging apparatus.
摘要翻译： 一种空袋供给方法和装置，首先用第一吸引构件提升空袋，其中袋底与第一定位塞接触并定位。 抬起的袋子然后用卡盘夹持并向上运送，使空袋子垂直，袋子底部朝上，然后一个第二抽吸部件吸引靠近袋口的空袋，并将其运送到带有口袋 向前，使得袋的袋口被第二定位塞止住并定位。 然后将空袋子由第三抽吸构件提起，由卡盘夹持并向上运送，使得袋子的姿态在袋口朝上的情况下变为垂直，然后将袋子转移到包装的夹具 仪器。
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公开(公告)号：US20080272507A1
公开(公告)日：2008-11-06
申请号：US12216451
申请日：2008-07-03
申请人： Hisashi Ukawa , Akio Yamane
发明人： Hisashi Ukawa , Akio Yamane
IPC分类号： B01J13/04
CPC分类号： G01N33/54393
摘要： In accordance with the present invention, active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups.Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
摘要翻译： 根据本发明，活性羧酸酯基团偶联在微球表面上，以减少微球加工方法，控制副反应，稳定保存含有活性羧酸酯基团的珠粒。 此外，在微球中用至少一个荧光染料笼标记的微球，并将微球保存在低级醇中。
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公开(公告)号：US5741638A
公开(公告)日：1998-04-21
申请号：US358995
申请日：1994-12-19
申请人： Akio Yamane
发明人： Akio Yamane
CPC分类号： C12Q1/6834 , C12N15/10 , C12Q1/6881
摘要： A microtiter well in which a single stranded nucleic acid including a plurality of sequences specifically hybridizalbe with a target nucleic acid is immobilized is disclosed. The single stranded nucleic acid is derived from a phage or a phage-plasmid. The microtiter well enables a target nucleic acid to be specifically detected with a high sensitivity and high efficiency and further enables the detection procedure to be automated.
摘要翻译： 公开了一种其中固定有包含与靶核酸特异性杂交的多个序列的单链核酸的微量滴定孔。 单链核酸来源于噬菌体或噬菌体质粒。 微量滴定孔能够以高灵敏度和高效率特异性检测靶核酸，并且进一步使检测程序能够自动化。
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公开(公告)号：US20130137095A1
公开(公告)日：2013-05-30
申请号：US13638216
申请日：2011-03-25
申请人： Shiro Kitano , Akio Yamane , Atsushi Maruyama
发明人： Shiro Kitano , Akio Yamane , Atsushi Maruyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6827 , C12Q2527/125 , C12Q2537/1373 , C12Q2545/107
摘要： A method for identifying a base sequence accompanying competitive hybridization that includes a thermal denaturation subjecting a sample double-stranded nucleic acid and a reference double-stranded nucleic acid containing the same base sequence as a target base sequence to thermal denaturation treatment in a single reaction solution, a temperature lowering carrying out competitive hybridization between the sample double-stranded nucleic acid and the reference double-stranded nucleic acid by lowering the temperature of the reaction solution after the thermal denaturation, a measurement measuring a double-stranded nucleic acid formed by a nucleic acid strand that composed the reference double-stranded nucleic acid and a nucleic acid strand that composed the sample double-stranded nucleic acid, and an identification identifying identity between the reference double-stranded nucleic acid and the sample double-stranded nucleic acid based on measurement results obtained from the measurement, the temperature lowering being carried out in the presence of a cationic comb-type polymer.
摘要翻译： 用于鉴定伴随竞争性杂交的碱基序列的方法，其包括将样品双链核酸和含有相同碱基序列的参考双链核酸作为靶碱基序列的热变性在单一反应溶液中进行热变性处理 ，通过降低热变性后的反应溶液的温度，进行样品双链核酸与参考双链核酸之间的竞争性杂交的降温，测量由核酸形成的双链核酸的测量 构成参考双链核酸的酸链和构成样品双链核酸的核酸链，以及基于测量的参考双链核酸和样品双链核酸之间的鉴定身份的鉴定 从测量结果得到的结果， 在阳离子梳型聚合物存在下进行降温。
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公开(公告)号：US20060292635A1
公开(公告)日：2006-12-28
申请号：US11438625
申请日：2006-05-23
申请人： Hisashi Ukawa , Akio Yamane
发明人： Hisashi Ukawa , Akio Yamane
CPC分类号： G01N33/54393
摘要： In accordance with the present invention, active carboxylic acid ester groups are coupled on the surfaces of microspheres so as to reduce protocols for microsphere processing, control side reactions, and stably preserve beads containing active carboxylic acid ester groups. Further, microspheres labeled with at least one fluorescent dye cage in the microspheres, and the microspheres are preserved in lower alcohol.
摘要翻译： 根据本发明，活性羧酸酯基团偶联在微球表面上，以减少微球加工方法，控制副反应，稳定保存含有活性羧酸酯基团的珠粒。 此外，在微球中用至少一个荧光染料笼标记的微球，并将微球保存在低级醇中。
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公开(公告)号：US4876335A
公开(公告)日：1989-10-24
申请号：US67798
申请日：1987-06-30
申请人： Akio Yamane , Tatsuro Kawasoe , Noriko Tsukumo , Kenichi Miyoshi
发明人： Akio Yamane , Tatsuro Kawasoe , Noriko Tsukumo , Kenichi Miyoshi
摘要： A poly-labelled oligonucleotide having a plurality of labels, comprising labels carried on a polylysine and having the polylysine introduced on the extension from 5'- and/or 3'-end through phosphate group is useful as a probe for hybridization.
摘要翻译： 具有多个标记的多标记寡核苷酸可用作杂交探针，所述多标记寡核苷酸包含在聚赖氨酸上携带的标记，并且在5'-和/或3'末端通过磷酸基的延伸引入聚赖氨酸。
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公开(公告)号：US09523119B2
公开(公告)日：2016-12-20
申请号：US13248321
申请日：2011-09-29
申请人： Akio Yamane , Mashimo Nakayama , Shiro Kitano
发明人： Akio Yamane , Mashimo Nakayama , Shiro Kitano
CPC分类号： C12Q1/686 , C12Q1/6858 , G01N2021/6441 , C12Q2537/161 , C12Q2535/131 , C12Q2531/119 , C12Q2525/186 , C12Q2527/125
摘要： The present invention relates to a method of distinguishing genotypes using PCR-PHFA including: a nucleic acid amplification step in which a mutation site-including region of a gene is amplified by a nucleic acid amplification reaction, thereby obtaining an amplification reaction solution; and a distinction step in which the amplification reaction solution obtained from the nucleic acid amplification step is mixed with a reference double-stranded nucleic acid having a specific genotype on the mutation site as well as being labeled with a labeling substance, and the mixture is subjected to a competitive strand displacement reaction, and the level of the occurrence of strand displacement is assessed so as to distinguish the identity; and the competitive strand displacement reaction is performed under a condition to suppress a polymerase extension reaction, and a genotype distinguishing kit for use in the distinct of genotypes by this method.
摘要翻译： 本发明涉及使用PCR-PHFA区分基因型的方法，包括：核酸扩增步骤，其中通过核酸扩增反应扩增基因的突变位点包含区域，从而获得扩增反应溶液; 以及将从核酸扩增步骤得到的扩增反应溶液与突变位点上具有特定基因型的参照双链核酸混合并用标记物质标记的鉴别步骤， 进行竞争性股份置换反应，评估股份置换的发生水平，以区分身份; 并且在抑制聚合酶延伸反应的条件下进行竞争性链置换反应，以及通过该方法在不同基因型中使用的基因型鉴别试剂盒。
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10.发明授权公开(公告)号：US06306603B1
公开(公告)日：2001-10-23
申请号：US09476124
申请日：2000-01-03
申请人： Takanori Oka , Akio Yamane , Takao Tanaka
发明人： Takanori Oka , Akio Yamane , Takao Tanaka
IPC分类号： C07H2104
CPC分类号： C07K14/70596 , C12Q1/6883 , C12Q2600/156
摘要： The present invention provides CD36 mutant gene and methods and kits for diagnosing diseases caused by a lipid metabolism abnormality.
摘要翻译： 本发明提供CD36突变基因以及用于诊断由脂质代谢异常引起的疾病的方法和试剂盒。
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