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公开(公告)号：US20060160084A1
公开(公告)日：2006-07-20
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of said sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of said sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac′), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between said sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 在本发明的方法中，引物在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）杂交的序列（Ac'）和5 所述序列（Ac'）的序列（B'）与位于靶核酸序列上的所述序列（A）的5'侧的序列（B）的互补序列（Bc）杂交， 其中{X-（Y-Y'）} / X在-1.00至1.00的范围内，其中X表示所述序列中的碱基数（Ac'），Y表示侧翼的区域中的碱基数 所述序列（A）和（B）在靶核酸序列中，Y'表示所述序列（Ac'）和（B'）之间的间插序列中的碱基数（Y'可以为零）。
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公开(公告)号：US07803579B2
公开(公告)日：2010-09-28
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of the sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of the sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac′), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between the sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 该方法包括提供一种引物，其在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）和5'侧杂交的序列（Ac'） 序列（Ac'）与位于靶核酸序列上的序列（A）的5'侧的序列（B）的互补序列（Bc）杂交的序列（B'），其中{X（ Y-Y'）} / X在-1.00至1.00的范围内，其中X表示序列中的碱基数（Ac'），Y表示侧翼为序列（A）的区域中的碱基数， 和（B），Y'表示序列（Ac'）和（B'）之间的间隔序列中的碱基数（Y'可以为零）。
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公开(公告)号：US20090042197A1
公开(公告)日：2009-02-12
申请号：US12094896
申请日：2006-11-21
CPC分类号： C12Q1/6844 , C12Q1/6865 , C12Q2527/125 , C12Q2525/301
摘要： Problem to be Solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.
摘要翻译： 待解决的问题提供了一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。
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公开(公告)号：US08097414B2
公开(公告)日：2012-01-17
申请号：US12094896
申请日：2006-11-21
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6865 , C12Q2527/125 , C12Q2525/301
摘要： Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.
摘要翻译： 待解决的问题提供一种方便，快速，有效地检测和/或扩增生物样品如血液或细胞中的核酸的方法。 溶液提供了一种检测样品中所含的核酸的方法，其包括将至少一种选自多酚，多元醇，糖酸，糖醇和亲水性可生物降解聚合物的物质加入到样品中的步骤， 将与要检测的核酸的一部分核酸序列互补的寡核苷酸互补结合到核酸序列的一部分的步骤和检测待检测的核酸的步骤。
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5.发明申请公开(公告)号：US20100221787A1
公开(公告)日：2010-09-02
申请号：US12739496
申请日：2008-10-24
申请人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
发明人： Yoshihide Hayashizaki , Masayoshi Itoh , Alexander Lezhava , Yoshimi Benno , Yasumasa Mitani , Hajime Kanamori
CPC分类号： C12Q1/6846 , C12N9/1252 , C12P19/34 , C12Q2525/155 , C12Q2521/107 , C12Q2527/101 , C12Q2531/107
摘要： A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO. 25 (X) a primer that contains, in a 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) of a 3′ end portion of the target nucleic acid sequence and also contains, on a 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5′ side with respect to the sequence (A) in the target nucleic acid sequence
摘要翻译： 提供了适用于特定等温扩增方法的DNA聚合酶和使用DNA聚合酶的等温扩增方法。 在包含下述（a）或（b）中所述的蛋白质的DNA聚合酶的存在下，使用下述第一引物等温进行靶核酸序列的扩增反应 X）。 通过使用DNA聚合酶，可以在比以往更短的时间内使用引物进行扩增反应。 （a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 23（b）具有SEQ ID NO：1所示的氨基酸序列的蛋白质。 25（X）引物，其在3'末端含有与目标核酸序列的3'端部分的序列（A）杂交的序列（Ac'），并且在5' 序列（Ac'）侧，与靶序列（A）相对于存在于5'侧的序列（B）的互补序列（Bc）杂交的序列（B'）
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公开(公告)号：US20060110745A1
公开(公告)日：2006-05-25
申请号：US11123223
申请日：2005-05-06
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2533/101 , C12Q2525/301 , C12Q2521/501 , C12Q2521/101
摘要： The invention provides a sequence specific method for amplifying nucleic acids. More particularly, the invention provides a method for amplifying nucleic acid sequences which enables such sequences to be detected with high precision, rapidity and high specificity as compared to conventional methods. The present invention also provides a simple method for cloning nucleic acids, particularly, a rapid and simple method for amplifying alternative splicing forms synthesized by an alternative splicing which is performed in a process of preparing a matured mRNA from a DNA.
摘要翻译： 本发明提供了用于扩增核酸的序列特异性方法。 更具体地，本发明提供了与常规方法相比，用于扩增能够以高精度，快速和高特异性检测这种序列的核酸序列的方法。 本发明还提供了用于克隆核酸的简单方法，特别是用于扩增通过在从DNA中制备成熟mRNA的过程中进行的选择性剪接合成的可变剪接形式的快速和简单的方法。
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7.发明授权Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same 有权扩增核酸的方法和使用该方法检测突变核酸的方法公开(公告)号：US08206902B2
公开(公告)日：2012-06-26
申请号：US10583706
申请日：2004-12-24
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101
摘要： A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) located in the 3′ end portion of the target nucleic acid sequence. The first primer also contains, on the 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5′ side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3′ end portion, a sequence (Cc′) that hybridizes to a sequence (C) located in the 3′ end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5′ side of the sequence (Cc′), a folded sequence (D-Dc′) that contains, on the same strand, two nucleic acid sequences that hybridize to each other.
摘要翻译： 引物组，其允许靶核酸被特异性和有效地扩增。 本发明的引物组包括允许靶核酸序列扩增的至少两个引物。 引物组中包含的第一引物在其3'末端含有与位于靶核酸序列3'端部分的序列（A）杂交的序列（Ac'）。 第一引物还在序列的5'侧包含与5'侧存在的序列（B）互补序列（Bc）杂交的序列（B'），相对于 涉及靶核酸序列中的序列（A）。 包括在引物组中的第二引物在其3'末端包含与位于与靶核酸序列的互补序列的3'端部分的序列（C）杂交的序列（Cc'）。 第二引物还包含在序列的5'侧（Cc'）上的折叠序列（D-Dc'），其在同一条链上含有彼此杂交的两个核酸序列。
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8.发明申请Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same 有权扩增核酸的方法和使用该方法检测突变核酸的方法公开(公告)号：US20070190531A1
公开(公告)日：2007-08-16
申请号：US10583706
申请日：2004-12-24
CPC分类号： C12Q1/6853 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q2537/163 , C12Q2531/113 , C12Q2521/514 , C12Q2525/301 , C12Q2525/161 , C12Q2537/125 , C12Q2522/101
摘要： A primer set that allows a target nucleic acid to be amplified specifically and efficiently. The primer set of the present invention includes at least two primers that allow a target nucleic acid sequence to be amplified. A first primer included in the primer set contains, in its 3′ end portion, a sequence (Ac′) that hybridizes to a sequence (A) located in the 3′ end portion of the target nucleic acid sequence. The first primer also contains, on the 5′ side of the sequence (Ac′), a sequence (B′) that hybridizes to a complementary sequence (Bc) to a sequence (B) that is present on the 5′ side with respect to the sequence (A) in the target nucleic acid sequence. A second primer included in the primer set contains, in its 3′ end portion, a sequence (Cc′) that hybridizes to a sequence (C) located in the 3′ end portion of a complementary sequence to the target nucleic acid sequence. The second primer also contains, on the 5′ side of the sequence (Cc′), a folded sequence (D-Dc′) that contains, on the same strand, two nucleic acid sequences that hybridize to each other.
摘要翻译： 引物组，其允许靶核酸被特异性和有效地扩增。 本发明的引物组包括允许靶核酸序列扩增的至少两个引物。 引物组中包含的第一引物在其3'末端含有与位于靶核酸序列3'端部分的序列（A）杂交的序列（Ac'）。 第一引物还在序列的5'侧包含与5'侧存在的序列（B）互补序列（Bc）杂交的序列（B'），相对于 涉及靶核酸序列中的序列（A）。 包括在引物组中的第二引物在其3'末端包含与位于与靶核酸序列的互补序列的3'端部分的序列（C）杂交的序列（Cc'）。 第二引物还包含在序列的5'侧（Cc'）上的折叠序列（D-Dc'），其在同一条链上含有彼此杂交的两个核酸序列。
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公开(公告)号：US20060194207A1
公开(公告)日：2006-08-31
申请号：US10566134
申请日：2004-07-29
CPC分类号： B01L3/502 , B01L3/5082 , B01L2200/0621 , B01L2300/0672 , B01L2300/0832 , B01L2300/087 , B01L2400/0677 , B01L2400/0683
摘要： The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for detecting a target nucleic acid from a sample, comprising at least a first compartment which contains an extraction reagent composition for extracting nucleic acids from said sample, a second compartment which contains an amplification reagent composition for amplifying the target nucleic acid, a separating means for separating the first and second compartments, and an aperture which enables to introduce said sample into only said first compartment. The separating means breaks the separation of the first and second compartments by physical energy supplied from the outside of the reactor, and thereby makes it possible to mix the extraction reagent composition in the first compartment and the amplification reagent composition in the second compartment.
摘要翻译： 本发明提供一种用于在单一容器中进行提取核酸和扩增核酸的步骤的反应器，所述步骤的步骤通常彼此不同。 根据本发明的反应器是用于从样品中检测靶核酸的反应器，其包含至少第一隔室，其包含用于从所述样品中提取核酸的提取试剂组合物，第二室含有用于 扩增目标核酸，用于分离第一和第二隔室的分离装置，以及能够将所述样品引入所述第一隔室的孔。 分离装置通过从反应器外部提供的物理能量破坏第一和第二隔室的分离，从而使得可以将第一隔室中的提取试剂组合物和第二隔室中的扩增试剂组合物混合。
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10.发明申请公开(公告)号：US20070003929A1
公开(公告)日：2007-01-04
申请号：US10538941
申请日：2003-12-12
CPC分类号： C12Q1/6827 , Y02A90/26 , C12Q2537/113 , C12Q2521/514 , C12Q2563/131 , C12Q2521/301 , C12Q2521/307
摘要： The present invention provides a method for identifying, analyzing and/or cloning nucleic acid isoforms comprising the steps of: preparing at least two nucleic acid isoforms, complementary to each other, hybridizing the at least two complementary nucleic acid isoforms and forming double strand RNA/RNA or DNA/DNA hybrids comprising unpaired regions; recovering the RNA/RNA or DNA/DNA hybrids comprising unpaired regions from not hybridized nucleic acids and from nucleic acids not comprising unpaired regions; and identifying, analyzing and/or cloning the recovered nucleic acid fragment comprising unpaired regions.
摘要翻译： 本发明提供了用于鉴定，分析和/或克隆核酸同种型的方法，其包括以下步骤：制备彼此互补的至少两个核酸同种型，将所述至少两种互补核酸同种型杂交并形成双链RNA / 包含不成对区域的RNA或DNA / DNA杂交体; 从未杂交的核酸和不包含未配对区域的核酸中回收包含不成对区域的RNA / RNA或DNA / DNA杂交体; 以及鉴定，分析和/或克隆包含不成对区域的回收的核酸片段。
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