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    shRNA library
    3.
    发明申请
    shRNA library 有权
    shRNA文库

    公开(公告)号：US20100029505A1

    公开(公告)日：2010-02-04

    申请号：US12221325

    申请日：2008-08-01

    申请人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    发明人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    IPC分类号： C40B30/06 ,  C40B40/08 ,  C40B50/06 ,  C12P19/34 ,  C40B40/02

    CPC分类号： C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    摘要： As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

    摘要翻译： 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

    shRNA library
    4.
    发明授权
    shRNA library 有权
    shRNA文库

    公开(公告)号：US07863222B2

    公开(公告)日：2011-01-04

    申请号：US12221325

    申请日：2008-08-01

    申请人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    发明人： Donald G. Payan ,  Mary Shen ,  Simon Yu ,  Todd M. Kinsella ,  Yasumichi Hitoshi

    IPC分类号： C40B50/06 ,  C40B40/08 ,  C40B30/06 ,  C40B40/02 ,  C12P19/34 ,  A61K49/12

    CPC分类号： C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06

    摘要： As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.

    摘要翻译： 如上所述，本公开的某些方面涉及核酸载体文库以及其制备方法。 在某些实施方案中，核酸载体文库包含：多个下式的核酸分子：S1-R-S2，其中在多个的每个核酸中，S1和S2的长度各自为至少15个核苷酸; S1和S2沿其整个长度彼此互补; S1或S2在其整个长度上与真核mRNA中的序列互补; R是限制性内切核酸酶的六碱基识别位点; 并且其中S1和S2在多个不同成员之间的核苷酸序列变化。 还提供了扩增环状核酸的方法。

    Methods and compositions for the identification of anti-poxvirus agents
    10.
    发明授权
    Methods and compositions for the identification of anti-poxvirus agents 有权
    用于鉴定抗痘病毒剂的方法和组合物

    公开(公告)号：US07132234B2

    公开(公告)日：2006-11-07

    申请号：US10975285

    申请日：2004-10-27

    申请人： Henry H. Lu ,  Jianing Huang ,  Donald G. Payan

    发明人： Henry H. Lu ,  Jianing Huang ,  Donald G. Payan

    IPC分类号： C12Q1/70 ,  C12Q1/48 ,  C12Q1/18 ,  C12N9/10 ,  A61K49/00

    CPC分类号： C12Q1/25 ,  G01N2333/065 ,  G01N2333/9015 ,  G01N2510/00

    摘要： The invention provides methods for identifying an anti-poxviral agents. In many embodiments, the methods involve contacting a poxviral p28 polypeptide with a candidate agent, and determining an effect of the agent on a ubiquitin ligase activity of the p28 polypeptide. The effect of the agent may be determined using a variety of different cell based or biochemical assays, such as polyubiquitylation assays and cell viability assays. The invention also provides methods for modulating poxvirus pathogenicity in a cell, and methods of treating an individual infected with a poxvirus. The subject methods find use in a variety of drug discovery, research and military applications.

    摘要翻译： 本发明提供鉴定抗痘病毒药物的方法。 在许多实施方案中，所述方法包括使痘病毒p28多肽与候选试剂接触，并确定试剂对p28多肽的泛素连接酶活性的影响。 可以使用多种不同的基于细胞或生物化学测定法，例如多泛素化测定和细胞活力测定来确定试剂的作用。 本发明还提供调节细胞中痘病毒致病性的方法，以及治疗感染痘病毒的个体的方法。 主题方法可用于各种药物发现，研究和军事应用。