公开(公告)号：US06238625B1

公开(公告)日：2001-05-29

申请号：US09216156

申请日：1998-12-18

申请人： Yong Keun Chang ,  Jong Dae Lee ,  Yong Ho Jung

发明人： Yong Keun Chang ,  Jong Dae Lee ,  Yong Ho Jung

IPC分类号： G01N2105

CPC分类号： G01N21/05

摘要： The present invention relates to a sealed high-density on-line measuring device for spectrophotometer whose cuvette holder is linked to a reactor, which comprises a base; a fixed plate for light passage, which is vertically placed on the base; a moving plate for light radiation, which is vertically placed on the base at an opposite side to the fixed plate so that it can slide on the base horizontally; inlet and outlet for sample passage, each of which is positioned at outer side of the moving plate; a metal ring linking the fixed plate and the moving plate; and, upper and lower shielders each of whose ends is in contact with the fixed plate and moving plate, respectively, to give a sealed space. In accordance with the scaled on-line measuring device of the invention, high concentrations of samples in solutions can be measured without dilution, by way of employing a newly designed cuvette holder in a spectrophotometer fitted to a reactor.

摘要翻译： 本发明涉及一种用于分光光度计的密封高密度在线测量装置，其比色杯支架连接到包括底座的反应器; 垂直放置在基座上的用于光通道的固定板; 用于光辐射的移动板，其在固定板的相对侧垂直放置在基座上，使得其能够在基座上水平滑动; 入口和出口用于样品通道，每个入口和出口位于移动板的外侧; 连接固定板和移动板的金属环; 以及其各自的端部分别与固定板和移动板接触的上部和下部护罩，以产生密封空间。 根据本发明的缩放的在线测量装置，可以在没有稀释的情况下测量溶液中的高浓度样品，通过在安装在反应器上的分光光度计中使用新设计的比色杯保持器。

公开(公告)号：US06204046B1

公开(公告)日：2001-03-20

申请号：US09261838

申请日：1999-03-03

申请人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

发明人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

IPC分类号： C12N120

CPC分类号： C12R1/01 ,  C10G32/00

摘要： The present invention relates to Gordona sp. CYKS1 (KCTC 0431BP) capable of selective removal of organically bound sulfurs from carbonaceous fossil fuel such as petroleum and coal by cleaving bonds between carbon and sulfur atoms in the said sulfur-containing organic compounds, and a method for biological desulfurization using this strain at the room temperature and atmospheric pressure. Since Gordona sp. CYKS1 (KCTC 0431BP) utilizes various organic sulfur compounds in fossil fuel besides dibenzothiophene as a sole sulfur source, the method for biological desulfurization employing the Gordona strain has advantages over the conventional chemical methods as followings: The desulfurization can be carried out at a mild condition; the cost for installation and operation of equipments can be reduced; and, the desulfurization of highly complex organic sulfur compounds can be realized.

摘要翻译： 本发明涉及Gordona sp。 CYKS1（KCTC 0431BP）能够通过裂解所述含硫有机化合物中的碳原子和硫原子之间的键，从含碳化石燃料如石油和煤中有机结合的硫选择性地除去，以及使用该菌株在生物脱硫中的方法 室温和大气压。 由于戈多纳 CYKS1（KCTC 0431BP）除二苯并噻吩以外的化石燃料中使用各种有机硫化合物作为唯一的硫源，使用戈多纳菌的生物脱硫方法比以往的化学方法具有以下优点：脱硫可在温和条件下进行 ; 降低设备安装运行成本; 并且可以实现高度复杂的有机硫化合物的脱硫。

公开(公告)号：US06596521B1

公开(公告)日：2003-07-22

申请号：US09529570

申请日：2000-04-13

申请人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

发明人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

IPC分类号： C12P740

CPC分类号： C12P7/46 ,  C12M23/58 ,  C12M29/18 ,  C12M41/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/48

摘要： The present invention provides a method for manufacturing organic acid by high-efficiency fermentation, which comprises the steps of continuous culture of organic acid bacteria and collection of organic acid produced from the culture employing a cell-recycle multiple-stage continuous fermentor with serially connected fermentors, each of which comprises a fermentor containing a ferment container, temperature controller, stirrer, and pH controller; pumps for efflux-circulation of media from the fermentor; and, cell separator for separation and circulation of media from the pumps. According to the present method, the high-concentration lactic acid of 90 g/L can be produced with the high productivity of 50 g/L/h, which can reduce the facility cost and production cost in the bulk manufacturing process. In addition, the present invention can be effectively applied to the production of other organic acids such as acetic acid, formic acid, citric acid, malic acid, maleic acid, fumaric acid, and succinic acid, which show the end-product inhibition.

摘要翻译： 本发明提供了一种通过高效发酵制造有机酸的方法，其包括以下步骤：连续培养有机酸细菌和收集由使用连续发酵罐的细胞循环多级连续发酵罐的培养物产生的有机酸 ，其中每个包含含有发酵容器，温度控制器，搅拌器和pH控制器的发酵罐; 用于来自发酵罐的介质的外排循环的泵; 以及用于从泵分离和循环介质的细胞分离器。 根据本方法，可以以50g / L / h的高生产率生产90g / L的高浓度乳酸，这可以降低批量制造过程中的设备成本和生产成本。 此外，本发明可以有效地应用于生产其它有机酸如乙酸，甲酸，柠檬酸，苹果酸，马来酸，富马酸和琥珀酸，这些有机酸显示出最终产物的抑制作用。

公开(公告)号：US06197570B1

公开(公告)日：2001-03-06

申请号：US09260979

申请日：1999-03-02

申请人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

发明人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

IPC分类号： C12N120

CPC分类号： C12R1/365 ,  C10G32/00

摘要： The present invention relates to Nocardia sp. CYKS2 (KCTC 0432 Br) capable of selective removal of organically bound sulfurs from carbonaceous fossil fuel such as petroleum and coal by cleaving bonds between carbon and sulfur atoms in the said sulfur-containing organic compounds, and a method for biological desulfurization using this strain at the room temperature and atmospheric pressure. Since Nocardia sp. CYKS2 (KCTC 0432 Bp) utilizes various organic sulfur compounds in fossil fuel besides dibenzothiophene as a sole sulfur source, the method for biological desulfurization employing the Nocardia strain has advantages over the conventional chemical methods as followings: The desulfurization can be carried out at a mild condition; the cost for installation and operation of equipments can be reduced; and, the desulfurization of highly complex organic sulfur compounds can be realized.

摘要翻译： 本发明涉及诺卡氏菌属 CYKS2（KCTC 0432 Br）能够通过在所述含硫有机化合物中裂解碳和硫原子之间的键，从含碳化石燃料如石油和煤中有机结合的硫选择性除去，以及使用该菌株的生物脱硫方法 室温和大气压力。 自诺卡氏菌属 CYKS2（KCTC 0432 Bp）除了二苯并噻吩以外，在化石燃料中使用各种有机硫化合物作为唯一的硫源，采用诺卡氏菌菌株的生物脱硫方法比以往的化学方法具有以下优点：脱硫可以在温和 条件; 降低设备安装运行成本; 并且可以实现高度复杂的有机硫化合物的脱硫。