SUBSTRATE FOR LAB-ON-A-CHIP
    1.
    发明申请
    SUBSTRATE FOR LAB-ON-A-CHIP 审中-公开
    基于实验室的芯片

    公开(公告)号:US20090297733A1

    公开(公告)日:2009-12-03

    申请号:US12540832

    申请日:2009-08-13

    IPC分类号: C23C14/28

    CPC分类号: G01N27/44747

    摘要: A lab-on-chip substrate includes a resin having a silicon content of 10% or less by weight as its base material and a hydrophilic polymer covalently bound onto the surface thereof by high-energy ray irradiation suitable for a protein-processing chip. The lab-on-chip substrate is resistant to washing and usable for an extended period of time without adsorption of proteins on the base material surface, i.e., a protein electrophoretic polymeric chip having a microchannel allowing high-accuracy analysis of trace amounts of proteins because of reduction in the amount of detection noise.

    摘要翻译: 片上芯片基板包括硅含量为10重量%以下的树脂作为其基材,以及通过适用于蛋白质加工芯片的高能射线照射共价结合到其表面上的亲水性聚合物。 芯片上的实验室衬底耐洗涤并且可在不在基材表面上吸附蛋白质的情况下使用,即具有微通道的蛋白质电泳聚合物芯片,允许对微量蛋白进行高精度分析,因为 检测噪声量减少。

    Substrate for labo-on-a-chip
    2.
    发明申请
    Substrate for labo-on-a-chip 审中-公开
    基板实验室芯片

    公开(公告)号:US20070178240A1

    公开(公告)日:2007-08-02

    申请号:US11587024

    申请日:2005-04-21

    IPC分类号: B05D3/02

    CPC分类号: G01N27/44747

    摘要: The present invention relates to a lab-on-chip substrate, comprising a resin having a silicon content of 10% or less by weight as its base material and a hydrophilic polymer covalently bound onto the surface thereof by high-energy ray irradiation, and in particular, to a protein-processing chip. The present invention provides a lab-on-chip substrate resistant to washing and usable for an extended period of time without adsorption of proteins on the base material surface, i.e., a protein electrophoretic polymeric chip having a microchannel allowing high-accuracy analysis of trace amounts of proteins because of reduction in the amount of detection noise.

    摘要翻译: 本发明涉及一种芯片上芯片基板,其包括硅含量为10重量%以下的树脂作为其基材,以及通过高能射线照射共价结合到其表面上的亲水性聚合物, 特别是蛋白质加工芯片。 本发明提供一种耐洗涤实验室的片上基片,并且可延长使用时间,而不会在基材表面上吸附蛋白质,即具有微通道的蛋白质电泳聚合物芯片,允许微量的高精度分析 的蛋白质,因为检测噪声量的减少。

    METHOD FOR PREPARING aRNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION
    5.
    发明申请
    METHOD FOR PREPARING aRNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION 审中-公开
    制备aRNA的方法和基因表达分析方法

    公开(公告)号:US20120196768A1

    公开(公告)日:2012-08-02

    申请号:US13499824

    申请日:2011-04-21

    IPC分类号: C40B30/04 C12P19/34

    摘要: A method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative includes an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5′-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol % and less than 25 mol % with respect to the total of uridine 5′-triphosphate (UTP) and AA-UTP.

    摘要翻译: 从用固定剂固定的组织或细胞提取的RNA样品制备用于基因表达分析的RNA的方法包括通过逆转录和体外转录的RNA样品的扩增步骤,氨基烯丙尿苷的比例 用于体外转录的核苷酸试剂中的5'-三磷酸(AA-UTP)相对于尿苷5'-三磷酸(UTP)和AA-UTP的总量不低于5mol%且小于25mol% 。

    Optical recording media
    7.
    发明授权
    Optical recording media 失效
    光记录媒体

    公开(公告)号:US5863702A

    公开(公告)日:1999-01-26

    申请号:US623553

    申请日:1996-03-28

    摘要: An optical recording medium having each of a land and a groove and comprises at least a recording layer, dielectric layers and reflection layer. Recording and erasing of data can be carried out on both land and groove by a phase change between amorphous and crystalline states. The mirror portion may have reflectance characteristics selected from at least one of a reflectance of more than 15% and no more than 35%, when in a crystalline state, and 10% or less, when in an amorphous state. Alternatively, additionally, the depth of the groove may be such as form an optical path length equal to 1/7 to 1/5 of the wavelength of the reproducing light and the photo-absorbance of the recording layer in the amorphous phase and the photo-absorbance in the crystalline phase may satisfy the following formula,Aa-Ac.ltoreq.10where Aa is the photo-absorbance (%) of the recording layer in the amorphous phase and Ac is the photo-absorbance (%) of the recording layer in the crystalline phase. In an optical recording method, an edge type recording system is provided on an optical recording medium in which recording and erasing are effected by a phase change between amorphous and crystalline states. Recorded marks are provided by pulse trains each comprising a number of recording power pulses and, additionally, a power pulse, after the last of the recording power pulses, of a power less than a erase power and of a duration in a range 1.1 to 6, times the duration of the last recording power pulse.

    摘要翻译: 一种光盘记录介质,其具有平台和凹槽中的每一个,并且至少包括记录层,电介质层和反射层。 数据的记录和擦除可以通过非晶态和结晶状态之间的相变在陆地和凹槽上进行。 当处于非晶状态时,镜部分可以具有选自反射率大于15%且不大于35%的反射率特性,当处于结晶状态时为10%或更小。 或者,此外,凹槽的深度可以是形成等于再现光的波长的光路长度等于+ E,fra 1/7 + EE至+ E,fra 1/5 + EE的光路长度, 无定形相中的记录层的吸光度和结晶相中的光吸收度可以满足下式:Aa-Ac其中Aa是非晶相中记录层的光吸收(%),Ac 是结晶相中记录层的光吸收(%)。 在光记录方法中,边缘型记录系统设置在光记录介质上,其中记录和擦除是通过非晶态和结晶态之间的相变实现的。 记录的标记由脉冲串提供,每个脉冲串包括多个记录功率脉冲,另外,在最后的记录功率脉冲之后的功率脉冲具有小于擦除功率的功率,并且持续时间在1.1至6的范围内 ,乘以最后记录功率脉冲的持续时间。