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公开(公告)号：US06461492B1
公开(公告)日：2002-10-08
申请号：US09443719
申请日：1999-11-19
IPC分类号： C25F702
CPC分类号： G01N21/645 , G01N21/6428 , G01N27/44721
摘要： A multi-capillary electrophoresis apparatus arranged with a fixed sample injection holder opposite a detection side holder within the same plane and an epi-optical detection system. A sample is injected sequentially and separated components are successively fed to the detection part for analysis by fluorescence. The epi-optical system adjusts the parallelism between the detection side holder and scanning axis of the detector. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.
摘要翻译： 一种多毛细管电泳装置，其具有与同一平面内的检测侧支架相对的固定样品注射保持器和外延光学检测系统。 依次注入样品，并将分离的组分依次送入检测部分进行荧光分析。 外延光学系统调节检测器侧检测器与检测器的扫描轴之间的平行度。 因此，毛细管电泳装置可以在不受拉曼散射或瑞利散射影响的情况下检测到作为标签结合到样品的荧光染料的荧光。
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2.发明授权公开(公告)号：US07195698B2
公开(公告)日：2007-03-27
申请号：US10318124
申请日：2002-12-13
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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3.发明授权公开(公告)号：US06517696B1
公开(公告)日：2003-02-11
申请号：US09426760
申请日：1999-10-26
IPC分类号： C25B900
CPC分类号： G01N27/44782 , G01N27/44743
摘要： An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
摘要翻译： 将样品板的电极板放置在电泳装置的主体上，同时将插头插入迁移高压线路连接孔并连接到高压配电电缆。 基板的每个孔插入井导向件的通孔中，并进一步压配合并接合到电极板的空腔中，用于将基板固定到电极板。 然后将样品引入基板的每个孔中，并将毛细管柱的末端浸入每个孔中以施加迁移电压并将样品电泳注入毛细管柱中。
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公开(公告)号：US6093300A
公开(公告)日：2000-07-25
申请号：US37005
申请日：1998-03-09
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44743 , Y10S436/809
摘要： A base plate is made of an insulating material, and comprised of a flat surface and a connector part connected therewith. A plurality of wells are vertically and transversely arranged on the surface of the base plate at regular intervals respectively, and the respective wells are provided with individual electrode patterns reaching the connector part from bottoms thereof through the surface of the base plate. The connector part is connected to an external high-voltage application part.
摘要翻译： 基板由绝缘材料制成，并且由平坦表面和与其连接的连接器部件组成。 多个孔分别以规则的间隔垂直和横向地布置在基板的表面上，并且各孔具有从其底部通过基板的表面到达连接器部分的单独的电极图案。 连接器部分连接到外部高压施加部件。
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公开(公告)号：US06383356B1
公开(公告)日：2002-05-07
申请号：US09448139
申请日：1999-11-24
IPC分类号： G02N2726
CPC分类号： G01N27/44704
摘要： A drain joint of a pump block is opened, a piston (19a) is pushed while a piston (13a) is fixed, for charging a passage between a Luer-Lok joint (17) and an intersection part as well as a passage between the intersection part and the drain joint with a buffer. Thereafter the piston (13a) is pushed while the piston (19a) is fixed, to charge a passage (7a) with a polymer. Then, the drain joint is closed, the piston (13a) is pushed and the piston (19a) is pulled in response to the amount of pushing, for charging the passage between the intersection point and the Luer-Lok joint (17) with the polymer. Thereafter, the piston (13a) is pushed while the piston (19a) is fixed, to charge a capillary column with the polymer.
摘要翻译： 打开泵块的排水接头，同时在固定活塞（13a）的同时推动活塞（19a），用于对路德洛接头（17）和交叉部分之间的通道以及通道 交叉部分和排水接头与缓冲器。 此后，在活塞（19a）被固定的同时推动活塞（13a），以便用聚合物对通道（7a）充电。 然后，关闭排水接头，推动活塞（13a），并且响应于推动量而拉动活塞（19a），用于对交点和鲁尔洛克接头（17）之间的通道进行充电， 聚合物。 此后，在固定活塞（19a）的同时推动活塞（13a），以便与聚合物一起为毛细管柱充电。
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公开(公告)号：US6120667A
公开(公告)日：2000-09-19
申请号：US6933
申请日：1998-01-14
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： After a sample stage moves and brings a sample titer plate toward and into contact with a capillary array end, a high voltage is applied across electrodes for a prescribed time for injecting samples. Thereafter an electrophoresis reservoir comes into contact with the capillary array end, for starting electrophoresis. When electrophoresed/separated DNA fragments pass through a detection part, an excitation/photoreceiving optical system is scanned so that photomultipliers detect fluorescence from four types of fluorescent materials labeling the samples. The excitation/photoreceiving optical system comprises an epi-optical system and a confocal optical system, and is scanned at a high speed.
摘要翻译： 在样品台移动并将样品滴定板朝向并与毛细管阵列端接触之后，在电极上施加高电压达预定时间以注射样品。 此后，电泳槽与毛细管阵列端接触，用于开始电泳。 当电泳/分离的DNA片段通过检测部分时，扫描激发/光接收光学系统，使得光电倍增管检测标记样品的四种类型的荧光材料的荧光。 激发/光接收光学系统包括外延光学系统和共焦光学系统，并且被高速扫描。
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公开(公告)号：US06783650B2
公开(公告)日：2004-08-31
申请号：US10126661
申请日：2002-04-22
IPC分类号： C02F140
CPC分类号： G01N21/645 , G01N21/6428 , G01N27/44721
摘要： A capillary cassette is made up of two separated holders, an array of capillaries and a tube. The capillaries that make up the array are inserted into the first holder, extend through second holder having a detection window and then the capillary array is aligned and then bundled and inserted into a heat shrinkable tube which covers positions to which filler is applied, the tube is heated and shrunk to bundle them in an airtight manner and inserted into a mounting member.
摘要翻译： 毛细管盒由两个分离的保持器，一组毛细管和一个管构成。 构成阵列的毛细管被插入第一保持器中，延伸穿过具有检测窗口的第二保持器，然后将毛细管阵列对准，然后捆扎并插入到覆盖施加填料的位置的热收缩管中，管 被加热和收缩以将它们以气密的方式捆扎并插入到安装构件中。
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8.发明授权公开(公告)号：US08017320B2
公开(公告)日：2011-09-13
申请号：US10554678
申请日：2008-05-14
IPC分类号： C12Q1/68
CPC分类号： G01N33/57446 , C12Q1/6886 , C12Q2600/112 , G01N2333/90203 , G01N2333/988 , G01N2800/56
摘要： Metastatic cancer cells originating from gastric cancer are detected by a method comprising the step of collecting a biological sample from a subject, the step of detecting the presence of at least either aldehyde dehydrogenase or dopa decarboxylase in the biological sample of the subject, and the step of determining that the possibility of containing metastatic cancer cells originating from the gastric cancer in the sample is high when at least either aldehyde dehydrogenase or dopa decarboxylase is present. By the use of these as markers for metastatic cancer cells originating from gastric cancer, the presence or absence of peritoneal metastasis in a gastric cancer patient can be detected rapidly and reliably, and data important for deciding whether intraperitoneal cancer chemotherapy should be applied is provided.
摘要翻译： 通过包括从受试者收集生物样品的步骤的方法检测源自胃癌的转移性癌细胞，检测受试者的生物样品中至少一种醛脱氢酶或多巴脱羧酶的存在的步骤，以及步骤 确定当存在至少一种醛脱氢酶或多巴脱羧酶时，含有样品中源自胃癌的转移性癌细胞的可能性高。 通过使用这些作为来自胃癌的转移性癌细胞的标记物，可以快速可靠地检测胃癌患者腹膜转移的存在或不存在，并且提供了决定是否应用腹膜内癌化疗的重要数据。
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公开(公告)号：US20100035249A1
公开(公告)日：2010-02-11
申请号：US12186009
申请日：2008-08-05
申请人： Yoshihide Hayashizaki , Piero Carninci , Mutsumi Katayama , Shintaro Katayama , Masayoshi Itoh , Matthias Harbers
发明人： Yoshihide Hayashizaki , Piero Carninci , Mutsumi Katayama , Shintaro Katayama , Masayoshi Itoh , Matthias Harbers
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6834 , C12Q1/6874 , C12Q2535/101 , C12Q2565/537 , C12Q2525/155
摘要： The present invention provides methods for the sequencing of all RNA species within an RNA sample, such as the RNA content obtained from a cell, a tissue, a living organism, or from an artificial source. RNA molecules within the samples are labeled in a RNA-specific manner prior to immobilization on a solid support. One label is used to mark the location of the RNA molecule on the solid support, whereas the second label is used to mark selectively the S′ end of full-length mRNA molecules. RNA molecules are sequenced while being bound to the solid support in one or more sequencing reactions, and sequences of individual RNA molecules can be forwarded to computational analysis for assembling sequence information from individual sequencing reads obtained from the same location on the solid support. Not only unsupervised expression profiling on a genome-wide scale, but also the direct analysis of RNA-RNA interactions become possible as revealed by the analysis of the sequencing information obtained along with genomic information.
摘要翻译： 本发明提供了用于对RNA样品中所有RNA物种进行测序的方法，例如从细胞，组织，活生物体或人造来源获得的RNA含量。 在固定在固体支持物上之前，将样品中的RNA分子以RNA特异性方式标记。 一个标签用于标记RNA分子在固体支持物上的位置，而第二个标记用于选择性标记全长mRNA分子的S'末端。 RNA分子在一个或多个测序反应中与固体支持物结合时进行测序，并且可将单个RNA分子的序列转发到计算分析，以从固体支持物上的相同位置获得的单独测序读数组装序列信息。 不仅在基因组范围内无监督的表达谱，而且通过分析与基因组信息一起获得的测序信息显示，RNA-RNA相互作用的直接分析也是可能的。
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公开(公告)号：US20060160084A1
公开(公告)日：2006-07-20
申请号：US10532975
申请日：2003-10-29
CPC分类号： C12Q1/6844 , C12Q2525/301
摘要： The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of said sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of said sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in said sequence (Ac′), Y denotes the number of bases in the region flanked by said sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between said sequences (Ac′) and (B′) (Y′ may be zero).
摘要翻译： 本发明涉及有效地合成或扩增包含靶核酸序列的核酸的方法。 在本发明的方法中，引物在其3'末端部分包含与目标核酸序列的3'末端部分的序列（A）杂交的序列（Ac'）和5 所述序列（Ac'）的序列（B'）与位于靶核酸序列上的所述序列（A）的5'侧的序列（B）的互补序列（Bc）杂交， 其中{X-（Y-Y'）} / X在-1.00至1.00的范围内，其中X表示所述序列中的碱基数（Ac'），Y表示侧翼的区域中的碱基数 所述序列（A）和（B）在靶核酸序列中，Y'表示所述序列（Ac'）和（B'）之间的间插序列中的碱基数（Y'可以为零）。
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