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1.发明申请RNA EXTRACTION METHOD, RNA EXTRACTION REAGENT, AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS 审中-公开RNA提取方法，RNA提取试剂和分析生物材料的方法公开(公告)号：US20090111114A1
公开(公告)日：2009-04-30
申请号：US12251375
申请日：2008-10-14
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/101
摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.
摘要翻译： 提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。 该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。 此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。
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2.发明申请RNA extraction method, RNA extraction reagent, and method for analyzing biological materials 审中-公开RNA提取法，RNA提取试剂，生物材料分析方法等公开(公告)号：US20050123965A1
公开(公告)日：2005-06-09
申请号：US10981521
申请日：2004-11-05
CPC分类号： C12N15/1006 , C12N15/101
摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.
摘要翻译： 提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。 该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。 此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。
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3.发明申请Method of recovering a plurality of nucleic acids by an identical stationary phase and an apparatus thereof 审中-公开通过相同的固定相回收多种核酸的方法及其装置公开(公告)号：US20050084886A1
公开(公告)日：2005-04-21
申请号：US10957711
申请日：2004-10-05
CPC分类号： G01N1/40 , G01N2035/1055 , Y10T436/143333 , Y10T436/255
摘要： The present invention absorbs and captures nucleic components from various specimens by a single stationary phase and finally recovers the nucleic components by eluting the captured nucleic components from the stationary phase. This invention enables recovery of nucleic components without reducing the concentrations of nucleic acids and detection of specific nucleic components such as specific viruses.
摘要翻译： 本发明通过单个固定相吸收和捕获来自各种样品的核酸成分，并且最终通过从所述固定相洗脱所捕获的核酸成分来回收核酸成分。 本发明能够回收核酸成分而不降低核酸的浓度和检测特定的核酸成分如特定病毒。
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公开(公告)号：US20110015381A1
公开(公告)日：2011-01-20
申请号：US12869519
申请日：2010-08-26
IPC分类号： C07H21/00
CPC分类号： G01N35/109 , B01F2215/0037 , B01L3/0275 , B01L2200/0631 , G01N35/0099 , G01N35/028 , G01N35/1004 , G01N2035/00237 , G01N2035/103 , G01N2035/1058 , Y10T436/255
摘要： An instrument and a method for conveniently collecting nucleic acids from a biological nucleic acid-containing sample are provided. A nucleic acid-capturing tip having silica-containing solid phases enclosed therein in such a state as being capable of coming into contact with a liquid, wherein the solid phases have a water-flowing regions and the average interval among solid phases in the water-flowing regions is regulated to 25 μm or less.
摘要翻译： 提供了从生物含核酸样品中方便地收集核酸的仪器和方法。 在能够与液体接触的状态下封入其中含有二氧化硅的固相的核酸捕获尖端，其中所述固相具有水流区域，并且水相中的固相之间的平均间隔， 流动区域调节至25μm以下。
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5.发明授权公开(公告)号：US06815215B2
公开(公告)日：2004-11-09
申请号：US09789567
申请日：2001-02-22
IPC分类号： G01N134
CPC分类号： G01N1/40 , G01N2035/1055 , Y10T436/143333 , Y10T436/255
摘要： The present invention absorbs and captures nucleic components from various specimens by a single stationary phase and finally recovers the nucleic components by eluting the captured nucleic components from the stationary phase. This invention enables recovery of nucleic components without reducing the concentrations of nucleic acids and detection of specific nucleic components such as specific viruses.
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公开(公告)号：US08183054B2
公开(公告)日：2012-05-22
申请号：US12869519
申请日：2010-08-26
IPC分类号： G01N1/18
CPC分类号： G01N35/109 , B01F2215/0037 , B01L3/0275 , B01L2200/0631 , G01N35/0099 , G01N35/028 , G01N35/1004 , G01N2035/00237 , G01N2035/103 , G01N2035/1058 , Y10T436/255
摘要： An instrument and a method for conveniently collecting nucleic acids from a biological nucleic acid-containing sample are provided. A nucleic acid-capturing tip having silica-containing solid phases enclosed therein in such a state as being capable of coming into contact with a liquid, wherein the solid phases have a water-flowing regions and the average interval among solid phases in the water-flowing regions is regulated to 25 μm or less.
摘要翻译： 提供了从生物含核酸样品中方便地收集核酸的仪器和方法。 在能够与液体接触的状态下封入其中含有二氧化硅的固相的核酸捕获尖端，其中所述固相具有水流区域，并且水相中的固相之间的平均间隔， 流动区域调节至25μm以下。
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公开(公告)号：US20070059751A1
公开(公告)日：2007-03-15
申请号：US11518175
申请日：2006-09-11
CPC分类号： C12N1/066 , C12N15/1006 , C12N15/1017
摘要： In a nucleic acid isolation method for a solid biological sample, since two or more kinds of instruments are used for biological sample disruption and nucleic acid isolation, the operations are complicated, thereby increasing the operating labor, prolonging the operation time, and deteriorating the property of a nucleic acid associated with the prolonged operation time. A sample stuck to the instrument for disruption during the sample disruption operation is not brought to the subsequent nucleic acid isolation operation, thereby causing a problem of reducing the nucleic acid isolation efficiency. In the present nucleic acid isolation method, a step of disrupting a biological sample and a step of isolating a nucleic acid released from the disrupted sample are conducted with one instrument. The nucleic acid isolation efficiency can be improved without losing a sample stuck to an instrument for sample disruption, and the operability can be improved by simplifying the operations.
摘要翻译： 在固体生物样品的核酸分离方法中，由于使用两种或更多种仪器进行生物样品破碎和核酸分离，操作复杂，从而增加操作劳动力，延长操作时间并降低其性能 与延长的手术时间相关的核酸。 在样品破碎操作期间粘附到仪器上以破坏的样品不会进入随后的核酸分离操作，从而导致降低核酸分离效率的问题。 在本发明的核酸分离方法中，使用一台仪器进行破坏生物样品的步骤和从被破坏的样品中分离释放的核酸的步骤。 可以提高核酸分离效率，而不会将样品粘附到用于样品破坏的仪器上，并且可以通过简化操作来提高可操作性。
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8.发明申请公开(公告)号：US20060252142A1
公开(公告)日：2006-11-09
申请号：US11418031
申请日：2006-05-05
CPC分类号： C12N15/1006
摘要： It is an objective of the present invention to isolate RNA from a sample containing nucleic acid by safe and convenient operations. As a result of intensive studies, inventors of the present invention have found that DNA is precipitated out by adding an organic solvent to a mixed solution of a sample containing DNA and RNA and a chaotropic agent, so that RNA remains soluble. The present invention relates to a method whereby a sample containing nucleic acid, a chaotropic agent, and an organic solvent are mixed, DNA is precipitated out, and the precipitate is separated from the mixed solution, such that RNA is isolated from the residual solution. In addition, in accordance with the present invention, RNA is allowed to come into contact with a silica-containing solid phase so as to be bound to the silica-containing solid phase without the addition of a reagent or the like to the residual solution. Further, it is also possible to isolate DNA from the precipitate. In accordance with the present invention, high-purity RNA can be isolated from a sample containing DNA and RNA by safe and convenient operations. In addition, it is possible to simultaneously isolate RNA and DNA from a single sample.
摘要翻译： 本发明的目的是通过安全和方便的操作从含有核酸的样品中分离RNA。 作为深入研究的结果，本发明的发明人已经发现，通过向含有DNA和RNA的样品和离液剂的混合溶液中加入有机溶剂使DNA沉淀出来，使得RNA保持溶解。 本发明涉及一种使含有核酸，离液剂和有机溶剂的样品混合的方法，使DNA沉淀出来，从混合溶液中分离出沉淀物，从残留溶液中分离出RNA。 此外，根据本发明，允许RNA与含二氧化硅的固相接触，以便将其与含二氧化硅的固相结合，而不向残留溶液中加入试剂等。 此外，还可以从沉淀物中分离DNA。 根据本发明，可以通过安全和方便的操作从含有DNA和RNA的样品中分离高纯度RNA。 此外，可以从单个样品中同时分离RNA和DNA。
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公开(公告)号：US20090099350A1
公开(公告)日：2009-04-16
申请号：US12216411
申请日：2008-07-03
CPC分类号： C12N15/1006 , G01F23/2921 , G01N2035/1025
摘要： According to this invention, an instrument and a method for nucleic acid isolation is realized, whereby it is possible to prevent, for example, adherence of a discharged solution upon dispersion of such solution to the vicinity of a nucleic acid isolation column outlet.A light source unit and a light-receiving unit that receives totally reflected light of the photosensor 41 for detecting the fluid level are placed on a site on the side surface of the nucleic acid isolation column 35, where the photosensor can detect the fluid level corresponding to a remaining liquid volume of approximately 10 ul. In addition, the light source unit of the photosensor 41 is positioned in a manner such that incident light is totally reflected from the interface between the nucleic acid isolation column 35 and air in the nucleic acid isolation column 35 and light is allowed to be incident in the solution when reaching the interface between the nucleic acid isolation column 35 and the solution in the nucleic acid isolation column 35. The light-receiving unit of the photosensor 41 is positioned in a manner such that it receives the totally reflected light and is fixed on the upper part of the nucleic-acid-adsorptive solid phase 24. The fluid level detection sensor is in communication with a syringe-driving motor 34 via a control unit 40 such that a syringe 31 is driven upward when the fluid level reaches a predetermined level, provided that a certain volume of the liquid remains in the nucleic acid isolation column 35. Accordingly, dispersion of the dispersed liquid is prevented.
摘要翻译： 根据本发明，实现了一种用于核酸分离的仪器和方法，由此可以防止例如在将这种溶液分散到核酸分离塔出口附近时排出的溶液的粘附。 接收用于检测液面的光传感器41的全反射光的光源单元和光接收单元被放置在核酸隔离柱35的侧表面上的位置，光电传感器可以检测相应的液面 至约10μl的剩余液体体积。 此外，光传感器41的光源单元被定位成使得入射光从核酸隔离柱35中的界面和核酸隔离柱35中的空气完全反射，并且允许光入射到 该溶液在到达核酸分离柱35和核酸隔离柱35中的溶液之间的界面时。光传感器41的光接收单元被定位成使得其接收全反射光并固定在 核酸吸附固相24的上部。液位检测传感器经由控制单元40与注射器驱动电动机34连通，使得当液面达到预定水平时注射器31向上驱动 条件是一定体积的液体残留在核酸分离柱35中。因此，防止分散液体的分散。
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公开(公告)号：US20060063180A1
公开(公告)日：2006-03-23
申请号：US11202063
申请日：2005-08-12
CPC分类号： C12N15/1006
摘要： It is an object of the present invention to release and isolate nucleic acid from a biological sample readily and in a short time. The present invention relates to a technique for isolating nucleic acid by which a biological sample including cells is caused to pass through a solid substance carrier to isolate the cells, a mixture of the cells and a cell lysis reagent is caused to pass through the solid substance carrier to disrupt the cells and release nucleic acid therein, and a mixture of the released nucleic acid and a nucleic acid binding reagent is caused to pass through the solid substance carrier to bind the nucleic acid to the solid substance carrier. Examples of an instrument comprising such solid substance carrier include an instrument comprising a syringe having a solid substance carrier fixed therein. The instrument is capable of moving a solution from one space to another space separated by the solid substance carrier, using pressurization and depressurization via the syringe, thereby causing the solution to pass through the solid substance carrier. Preferably, the solid substance carrier is a solid substance having a multitude of channels therein or a mesh-like solid substance composed of fibrous substances, by which cells are disrupted from a biological sample including the cells, nucleic acid is released, and the nucleic acid can be bound.
摘要翻译： 本发明的目的是容易且短时间地从生物样品中释放和分离核酸。 本发明涉及一种用于分离核酸的技术，通过该技术使包含细胞的生物样品通过固体物质载体以分离细胞，使细胞和细胞裂解试剂的混合物通过固体物质 载体以破坏细胞并释放其中的核酸，并且使释放的核酸和核酸结合试剂的混合物通过固体物质载体以将核酸结合至固体物质载体。 包括这种固体物质载体的仪器的实例包括一种仪器，其包括固定有固体物质载体的注射器。 该仪器能够通过经由注射器的加压和减压将溶液从一个空间移动到由固体物质载体分离的另一个空间，从而使溶液通过固体物质载体。 优选地，固体物质载体是其中具有多个通道的固体物质或由纤维物质组成的网状固体物质，细胞从包括细胞的生物样品中被破坏，核酸被释放，并且核酸 可以束缚
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