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53 条记录 (耗时 0.032 秒)

    POLYPEPTIDE BEING CAPABLE OF INCREASING THE PRODUCTION OF L-METHIONINE, A MICROORGANISM THAT OVEREXPRESSES SAID POLYPEPTIDE AND A PROCESS OF PREPARING L-METHIONINE IN HIGH YIELD USING SAME
    5.
    发明申请
    POLYPEPTIDE BEING CAPABLE OF INCREASING THE PRODUCTION OF L-METHIONINE, A MICROORGANISM THAT OVEREXPRESSES SAID POLYPEPTIDE AND A PROCESS OF PREPARING L-METHIONINE IN HIGH YIELD USING SAME 审中-公开
    可以增加L-甲硫氨酸的生产的多肽，超过表达多肽的微生物和使用它们在高产量中制备L-甲硫氨酸的方法

    公开(公告)号：US20100062498A1

    公开(公告)日：2010-03-11

    申请号：US12521541

    申请日：2007-12-28

    申请人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

    发明人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

    IPC分类号： C12P13/12 ,  C07K2/00 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12P13/12 ,  C07K14/245

    摘要： The present invention relates to a polypeptide capable of increasing the production of L-methionine in a microorganism. In particular, the present invention relates to an YgaZ and YgaH polypeptide or a complex thereof, referred to herein as YgaZH polypeptide, which are novel putative L-methionine exporters, polynucleotides encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing L-methionine and/or S-adenosyl-methionine, comprising the steps of culturing the transformed microorganism to produce L-methionine and/or S-adenosyl-methionine, and isolating L-methionine and/or S-adenosyl-methionine. The transformed microorganism of the present invention produces L-methionine in a high yield, thereby being used for medicinal and pharmaceutical industries and feed industry, in particular, animal feeds

    摘要翻译： 本发明涉及能够增加微生物中L-甲硫氨酸生产的多肽。 特别地，本发明涉及本文称为YgaZH多肽的YgaZ和YgaH多肽或其复合物，其为新型推定的L-甲硫氨酸出口商，其编码该多核苷酸的多核苷酸，包含该多核苷酸的重组载体， 重组载体和L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸的制备方法，包括培养转化的微生物以产生L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸并分离L-甲硫氨酸和/ 或S-腺苷 - 甲硫氨酸。 本发明的转化微生物以高产率生产L-甲硫氨酸，从而用于药用和制药工业和饲料工业，特别是动物饲料

    Method for L-threonine production
    7.
    发明授权
    Method for L-threonine production 有权
    L-苏氨酸生产方法

    公开(公告)号：US07378267B1

    公开(公告)日：2008-05-27

    申请号：US10508728

    申请日：2002-05-16

    申请人： Jae Yong Park ,  Byoung Choon Lee ,  Dae Cheol Kim ,  Jin Ho Lee ,  Jae Yong Cho ,  Young Hoon Park

    发明人： Jae Yong Park ,  Byoung Choon Lee ,  Dae Cheol Kim ,  Jin Ho Lee ,  Jae Yong Cho ,  Young Hoon Park

    IPC分类号： C12N1/12 ,  C12P13/04

    CPC分类号： C12P13/08

    摘要： A method for producing L-threonine using a microorganism is provided. In the method, the threonine dehydratase (tdc) gene existing in the genomic DNA of the microorganism is partially deactivated using a recombination technique. For a microorganism strain with enhanced activity of threonine operon-containing enzymes and the phosphoenolpyruvate carboxylase (ppc) gene, the tdc gene engaged in one of the four threonine metabolic pathways is specifically deactivated, thereby markedly increasing the yield of L-threonine.

    摘要翻译： 提供了使用微生物生产L-苏氨酸的方法。 在该方法中，存在于微生物的基因组DNA中的苏氨酸脱水酶（tdc）基因使用重组技术部分失活。 对于具有苏氨酸操纵子的酶和磷酸烯醇丙酮酸羧化酶（ppc）基因的活性增强的微生物菌株，特异性地去除了四种苏氨酸代谢途径之一的tdc基因，从而显着增加了L-苏氨酸的产量。

    Method of Isolating 1,3-Propanediol or 1,3-Propanediol and 1,2-Propanediol from Solution Containing 1,3-Propanediol, 1,2-Propanediol, Glycerol, and Glucose
    9.
    发明申请
    Method of Isolating 1,3-Propanediol or 1,3-Propanediol and 1,2-Propanediol from Solution Containing 1,3-Propanediol, 1,2-Propanediol, Glycerol, and Glucose 有权
    从1,3-丙二醇，1,2-丙二醇，甘油和葡萄糖的溶液中分离1,3-丙二醇或1,3-丙二醇和1,2-丙二醇的方法

    公开(公告)号：US20080097130A1

    公开(公告)日：2008-04-24

    申请号：US11574515

    申请日：2005-09-01

    申请人： Young Hoon Park ,  Kwang-Myung Cho ,  Seong-Uk Kang ,  Jin-Hyun Kim ,  Mi-Hae Cho

    发明人： Young Hoon Park ,  Kwang-Myung Cho ,  Seong-Uk Kang ,  Jin-Hyun Kim ,  Mi-Hae Cho

    IPC分类号： C07C29/74

    CPC分类号： C07C29/86 ,  C07C31/205

    摘要： A method of isolating 1,3-propanediol from a solution containing 1,3-propanediol, 1,2-propanediol, glycerol, and glucose is provided. The method includes: obtaining a concentrate by concentrating the solution via reduced pressure evaporation; dissolving the concentrate in a solvent selected from the group consisting of ethyl acetate, methyl ethyl ketone, and a mixture thereof and leaving the solution alone to fractionate the compounds in a solvent layer and a water layer; and loading the solvent layer in a silica-filled column under a low pressure liquid chromatography condition and eluting the solvent layer with a mixed solvent of methanol and at least one solvent which is miscible with methanol and has a polarity lower than methanol.

    摘要翻译： 提供了从含有1,3-丙二醇，1,2-丙二醇，甘油和葡萄糖的溶液中分离1,3-丙二醇的方法。 该方法包括：通过减压蒸发浓缩溶液获得浓缩物; 将浓缩物溶解在选自乙酸乙酯，甲基乙基酮及其混合物的溶剂中，并单独留下溶液以在溶剂层和水层中分离出化合物; 并在低压液相色谱条件下将溶剂层装入二氧化硅填充柱中，并用甲醇和至少一种与甲醇混溶并且极性低于甲醇的溶剂的混合溶剂洗脱溶剂层。

    Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell
    10.
    发明授权
    Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell 有权
    来自白色念珠菌的甘油-3-磷酸酯酶和甘油-3-磷酸脱氢酶，编码相同基因的载体和宿主细胞，以及使用宿主细胞产生甘油的方法

    公开(公告)号：US07351560B2

    公开(公告)日：2008-04-01

    申请号：US10558764

    申请日：2004-05-28

    申请人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Um

    发明人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Um

    IPC分类号： C12P7/20 ,  C12N1/21 ,  C12N15/63 ,  C12N9/04 ,  C12N9/16 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12N9/16 ,  C12P7/20

    摘要： Provided are a polypeptide having glycerol-3-phosphate dehydrogenase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 1 and a polypeptide having glycerol-3-phosphate phosphatase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 2. Provided is also a method for producing glycerol, which includes: culturing a host cell transformed with a vector containing a polynucleotide including a first polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 1 and a second polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 2 which are operably linked to a suitable regulatory sequence; and recovering glycerol from the culture.

    摘要翻译： 提供了具有甘油-3-磷酸脱氢酶活性的多肽和与SEQ ID NO：1所示的氨基酸序列具有80％或更高同源性的多肽和具有甘油-3-磷酸磷酸酶活性的多肽和80％以上的多肽 提供了与序列号2所示的氨基酸序列的同源性。提供了一种生产甘油的方法，其包括：培养用含有多核苷酸转化的宿主细胞，所述载体含有编码氨基酸序列的第一多核苷酸， 编码SEQ ID NO：2所示的氨基酸序列的第二多核苷酸，其编码可操作地连接到合适的调节序列; 并从培养物中回收甘油。