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    Method of producing 1,2-propanediol using Klebsiella pneumoniae
    1.
    发明授权
    Method of producing 1,2-propanediol using Klebsiella pneumoniae 有权
    使用肺炎克雷伯杆菌生产1,2-丙二醇的方法

    公开(公告)号：US07049109B2

    公开(公告)日：2006-05-23

    申请号：US10815882

    申请日：2004-03-31

    申请人： Young Hoon Park ,  Jin Su Chung ,  Kwang Myung Cho ,  Seong Uk Kang ,  Hye Won Um

    发明人： Young Hoon Park ,  Jin Su Chung ,  Kwang Myung Cho ,  Seong Uk Kang ,  Hye Won Um

    IPC分类号： C12P7/04

    CPC分类号： C12P7/18 ,  Y10S435/852

    摘要： A method of producing 1,2-propandiol is provided. The method includes incubating Klebsiella pneumoniae in a medium containing 10–30 g/L of a sugar carbon source excluding 6-deoxyhexose, in aerobic conditions; and separating 1,2-propandiol from the cultures. Using the method, 1,2-propandiol can be produced with a high yield by incubating Klebsiella pneumoniae in a medium containing a cheaper sugar carbon source.

    摘要翻译： 提供了1,2-丙二醇的制备方法。 该方法包括在需氧条件下，将含有10-30g / L不含6-脱氧己糖的糖碳源的肺炎克雷伯杆菌培养物; 并从培养物中分离1,2-丙二醇。 使用该方法，可以通过在含有较便宜的糖碳源的培养基中培养肺炎克雷伯杆菌而以高产率生产1,2-丙二醇。

    Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell
    2.
    发明授权
    Glycerol-3-phosphate phosphatase and glycerol-3-phosphate dehydrogenase from Candida albicans, genes encoding the same, vector and host cell containing the genes, and method for producing glycerol using the host cell 有权
    来自白色念珠菌的甘油-3-磷酸酯酶和甘油-3-磷酸脱氢酶，编码相同基因的载体和宿主细胞，以及使用宿主细胞产生甘油的方法

    公开(公告)号：US07351560B2

    公开(公告)日：2008-04-01

    申请号：US10558764

    申请日：2004-05-28

    申请人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Um

    发明人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Um

    IPC分类号： C12P7/20 ,  C12N1/21 ,  C12N15/63 ,  C12N9/04 ,  C12N9/16 ,  C07H21/04

    CPC分类号： C12N9/0006 ,  C12N9/16 ,  C12P7/20

    摘要： Provided are a polypeptide having glycerol-3-phosphate dehydrogenase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 1 and a polypeptide having glycerol-3-phosphate phosphatase activity and a 80% or more homology to an amino acid sequence as set forth in SEQ ID NO: 2. Provided is also a method for producing glycerol, which includes: culturing a host cell transformed with a vector containing a polynucleotide including a first polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 1 and a second polynucleotide encoding an amino acid sequence as set forth in SEQ ID NO: 2 which are operably linked to a suitable regulatory sequence; and recovering glycerol from the culture.

    摘要翻译： 提供了具有甘油-3-磷酸脱氢酶活性的多肽和与SEQ ID NO：1所示的氨基酸序列具有80％或更高同源性的多肽和具有甘油-3-磷酸磷酸酶活性的多肽和80％以上的多肽 提供了与序列号2所示的氨基酸序列的同源性。提供了一种生产甘油的方法，其包括：培养用含有多核苷酸转化的宿主细胞，所述载体含有编码氨基酸序列的第一多核苷酸， 编码SEQ ID NO：2所示的氨基酸序列的第二多核苷酸，其编码可操作地连接到合适的调节序列; 并从培养物中回收甘油。

    POLYPEPTIDE BEING CAPABLE OF INCREASING THE PRODUCTION OF L-METHIONINE, A MICROORGANISM THAT OVEREXPRESSES SAID POLYPEPTIDE AND A PROCESS OF PREPARING L-METHIONINE IN HIGH YIELD USING SAME
    3.
    发明申请
    POLYPEPTIDE BEING CAPABLE OF INCREASING THE PRODUCTION OF L-METHIONINE, A MICROORGANISM THAT OVEREXPRESSES SAID POLYPEPTIDE AND A PROCESS OF PREPARING L-METHIONINE IN HIGH YIELD USING SAME 审中-公开
    可以增加L-甲硫氨酸的生产的多肽，超过表达多肽的微生物和使用它们在高产量中制备L-甲硫氨酸的方法

    公开(公告)号：US20100062498A1

    公开(公告)日：2010-03-11

    申请号：US12521541

    申请日：2007-12-28

    申请人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

    发明人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

    IPC分类号： C12P13/12 ,  C07K2/00 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12P13/12 ,  C07K14/245

    摘要： The present invention relates to a polypeptide capable of increasing the production of L-methionine in a microorganism. In particular, the present invention relates to an YgaZ and YgaH polypeptide or a complex thereof, referred to herein as YgaZH polypeptide, which are novel putative L-methionine exporters, polynucleotides encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing L-methionine and/or S-adenosyl-methionine, comprising the steps of culturing the transformed microorganism to produce L-methionine and/or S-adenosyl-methionine, and isolating L-methionine and/or S-adenosyl-methionine. The transformed microorganism of the present invention produces L-methionine in a high yield, thereby being used for medicinal and pharmaceutical industries and feed industry, in particular, animal feeds

    摘要翻译： 本发明涉及能够增加微生物中L-甲硫氨酸生产的多肽。 特别地，本发明涉及本文称为YgaZH多肽的YgaZ和YgaH多肽或其复合物，其为新型推定的L-甲硫氨酸出口商，其编码该多核苷酸的多核苷酸，包含该多核苷酸的重组载体， 重组载体和L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸的制备方法，包括培养转化的微生物以产生L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸并分离L-甲硫氨酸和/ 或S-腺苷 - 甲硫氨酸。 本发明的转化微生物以高产率生产L-甲硫氨酸，从而用于药用和制药工业和饲料工业，特别是动物饲料

    Method for producing lactic acid with high concentration and high yield using lactic acid bacteria
    4.
    发明授权
    Method for producing lactic acid with high concentration and high yield using lactic acid bacteria 有权
    使用乳酸菌生产高浓度和高产量乳酸的方法

    公开(公告)号：US07682814B2

    公开(公告)日：2010-03-23

    申请号：US10582389

    申请日：2004-11-18

    申请人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Kim ,  Dae Chol Kim

    发明人： Young Hoon Park ,  Kwang Myung Cho ,  Hye Won Kim ,  Dae Chol Kim

    IPC分类号： C12N1/00 ,  C12N1/20

    CPC分类号： C12N1/20 ,  C12P7/56 ,  C12R1/225

    摘要： The present invention relates to a method for producing lactic acid with high concentration and high yield using Lactobacillus paracasei CJLA0310 KCCM-10542 that is separated and identified from Kimchi. Lactic acid is a very important organic acid with a wide range of applications including food additive such as food preservative, condiment or acidifier, and industrial fields such as cosmetics, chemistry, metals, electronics, fabrics, dyeing textiles, and pharmaceutical industries. Particularly, lactic acid is an essential ingredient of polylactic acid, one of biodegradable plastics to replace recalcitrant non-biodegradable plastics which are main causes of environmental contamination.

    摘要翻译： 本发明涉及使用从泡菜分离鉴定的副干酪乳杆菌CJLA0310 KCCM-10542来生产高浓度和高产量的乳酸的方法。 乳酸是一种非常重要的有机酸，适用范围广泛，包括食品添加剂如食品防腐剂，调味品或酸化剂，以及化妆品，化学，金属，电子，织物，染色纺织品和制药工业等工业领域。 特别地，乳酸是聚乳酸的重要成分，聚乳酸是能够替代作为环境污染的主要原因的顽固性不可生物降解塑料的生物降解塑料之一。