公开(公告)号：US20090325243A1

公开(公告)日：2009-12-31

申请号：US12308810

申请日：2007-06-26

申请人： Young Hoon Park ,  Kwang Myung Cho ,  Yong Uk Shin ,  Hyun Ae Bae ,  Jin Sook Chang ,  Jae Yeong Ju

发明人： Young Hoon Park ,  Kwang Myung Cho ,  Yong Uk Shin ,  Hyun Ae Bae ,  Jin Sook Chang ,  Jae Yeong Ju

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12N1/20

CPC分类号： C12P13/08 ,  C07K14/245 ,  C12N1/32 ,  C12P13/04 ,  C12P13/12

摘要： The present invention relates to an amino acid-producing microorganism capable of simultaneously utilizing glycerol as a carbon source, a method for preparing the microorganism, and a method for producing amino acids using the microorganism. According to the present invention, amino acids can be efficiently produced using a byproduct of biodiesel production, glycerol, thereby substituting a cheaper material for the conventional fermentation materials such as glucose.

摘要翻译： 本发明涉及能够同时利用甘油作为碳源的氨基酸生产微生物，微生物的制备方法和使用该微生物的氨基酸的制造方法。 根据本发明，可以使用生物柴油生产的副产物甘油有效地生产氨基酸，从而用较便宜的原料代替常规发酵材料如葡萄糖。

公开(公告)号：US09689009B2

公开(公告)日：2017-06-27

申请号：US13278106

申请日：2011-10-20

申请人： Jin Sook Chang ,  Jae Hyun Jo ,  Hyun Ae Bae ,  Byeong Cheol Song ,  Sol Kim ,  Hye Won Kim ,  Hye Won Um ,  Sung Hoo Jhon ,  Yong Uk Shin ,  Eun Bin Yang ,  Kyoung Min Lee ,  Soo An Shin

发明人： Jin Sook Chang ,  Jae Hyun Jo ,  Hyun Ae Bae ,  Byeong Cheol Song ,  Sol Kim ,  Hye Won Kim ,  Hye Won Um ,  Sung Hoo Jhon ,  Yong Uk Shin ,  Eun Bin Yang ,  Kyoung Min Lee ,  Soo An Shin

IPC分类号： C12P13/12 ,  C12P13/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77

CPC分类号： C12P13/12 ,  C12N9/1085 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77 ,  C12P13/06 ,  C12Y205/01065 ,  C12Y301/03003

摘要： The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and the activity of PhnC, PhnD, and PhnE is reduced, and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having these reduced and enhanced properties noted above are also provided herein.

公开(公告)号：US20100062498A1

公开(公告)日：2010-03-11

申请号：US12521541

申请日：2007-12-28

申请人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

发明人： Young Hoon Park ,  Kwang Myung Cho ,  So Young Kim ,  Yong Uk Shin ,  Hye Won Um

IPC分类号： C12P13/12 ,  C07K2/00 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12P13/12 ,  C07K14/245

摘要： The present invention relates to a polypeptide capable of increasing the production of L-methionine in a microorganism. In particular, the present invention relates to an YgaZ and YgaH polypeptide or a complex thereof, referred to herein as YgaZH polypeptide, which are novel putative L-methionine exporters, polynucleotides encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing L-methionine and/or S-adenosyl-methionine, comprising the steps of culturing the transformed microorganism to produce L-methionine and/or S-adenosyl-methionine, and isolating L-methionine and/or S-adenosyl-methionine. The transformed microorganism of the present invention produces L-methionine in a high yield, thereby being used for medicinal and pharmaceutical industries and feed industry, in particular, animal feeds

摘要翻译： 本发明涉及能够增加微生物中L-甲硫氨酸生产的多肽。 特别地，本发明涉及本文称为YgaZH多肽的YgaZ和YgaH多肽或其复合物，其为新型推定的L-甲硫氨酸出口商，其编码该多核苷酸的多核苷酸，包含该多核苷酸的重组载体， 重组载体和L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸的制备方法，包括培养转化的微生物以产生L-甲硫氨酸和/或S-腺苷 - 甲硫氨酸并分离L-甲硫氨酸和/ 或S-腺苷 - 甲硫氨酸。 本发明的转化微生物以高产率生产L-甲硫氨酸，从而用于药用和制药工业和饲料工业，特别是动物饲料

公开(公告)号：US07256018B2

公开(公告)日：2007-08-14

申请号：US11049844

申请日：2005-02-04

申请人： Young Hoon Park ,  Byoung Choon Lee ,  Jae Yong Park ,  Kwang Myung Cho ,  Yong Uk Shin

发明人： Young Hoon Park ,  Byoung Choon Lee ,  Jae Yong Park ,  Kwang Myung Cho ,  Yong Uk Shin

IPC分类号： C12P1/00 ,  C12P21/04

CPC分类号： C12P13/08

摘要： Provided are a microorganism capable of producing L-threonine and having an inactivated tyrR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.

摘要翻译： 提供能够产生L-苏氨酸并具有失活的tyrR基因的微生物，其制备方法和使用该微生物生产L-苏氨酸的方法。 该微生物可用于以高产率生产L-苏氨酸。

公开(公告)号：US07229794B2

公开(公告)日：2007-06-12

申请号：US11049845

申请日：2005-02-04

申请人： Young Hoon Park ,  Byoung Choon Lee ,  Kwang Myung Cho ,  Dae Cheol Kim ,  Yong Uk Shin ,  Jin Ho Lee

发明人： Young Hoon Park ,  Byoung Choon Lee ,  Kwang Myung Cho ,  Dae Cheol Kim ,  Yong Uk Shin ,  Jin Ho Lee

IPC分类号： C12P1/00 ,  C12P21/04

CPC分类号： C12P13/08 ,  C07K14/245

摘要： Provided are a microorganism capable of producing L-threonine and having an inactivated galR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.

摘要翻译： 提供能够产生L-苏氨酸并具有失活的galR基因的微生物，其生产方法和使用该微生物生产L-苏氨酸的方法。 该微生物可用于以高产率生产L-苏氨酸。

公开(公告)号：US08609396B2

公开(公告)日：2013-12-17

申请号：US12550099

申请日：2009-08-28

申请人： So Young Kim ,  Yong Uk Shin ,  In Kyung Heo ,  Hyun Ah Kim ,  Chang Il Seo ,  Ju Eun Kim ,  Sung Kwang Son ,  Sang Mok Lee ,  Sung Hoo Jhon ,  Han Jin Lee ,  Kwang Ho Na

发明人： So Young Kim ,  Yong Uk Shin ,  In Kyung Heo ,  Hyun Ah Kim ,  Chang Il Seo ,  Ju Eun Kim ,  Sung Kwang Son ,  Sang Mok Lee ,  Sung Hoo Jhon ,  Han Jin Lee ,  Kwang Ho Na

IPC分类号： C12N1/21 ,  C12P13/06

CPC分类号： C12N9/1205 ,  C12N9/93 ,  C12P13/06 ,  C12Y207/01033 ,  C12Y602/01001

摘要： Disclosed herein are a microorganism strain capable of producing the L-methionine precursor O-acetyl homoserine in high yield and a method of producing O-acetyl homoserine using the same. The microorganism strain is a strain of Escherichia sp. in which an acetyl-CoA synthase gene (acs) and/or a pantothenate kinase gene (coaA) encoding a pantothenate kinase gene refractory to feedback inhibition by CoA is introduced and expressed.

摘要翻译： 本文公开了能够以高产率生产L-甲硫氨酸前体O-乙酰高丝氨酸的微生物菌株和使用其的O-乙酰高丝氨酸的生产方法。 微生物菌株是大肠杆菌属的菌株。 其中引入并表达了编码由CoA反馈抑制难治的泛酸激酶基因的乙酰辅酶A合酶基因（acs）和/或泛酸激酶基因（coaA）。

公开(公告)号：US20070161447A1

公开(公告)日：2007-07-12

申请号：US11507750

申请日：2006-08-22

申请人： Yong Uk Shin ,  Sung Ho Shin

发明人： Yong Uk Shin ,  Sung Ho Shin

IPC分类号： F16G5/16 ,  F16G1/00 ,  F16G1/21

CPC分类号： F16G5/16

摘要： A belt for a continuously variable transmission, and a continuously variable transmission including the same, includes a plurality of clips, each defining a space which defines a groove to which a ring is inserted, thereby reducing slip.

摘要翻译： 用于无级变速器的皮带和包括该皮带的无级变速器包括多个夹子，每个夹具限定了一个限定了环的凹槽的空间，从而减少了滑动。

公开(公告)号：US09080155B2

公开(公告)日：2015-07-14

申请号：US13997054

申请日：2011-12-21

申请人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  Sung Kwang Son ,  In Kyung Heo ,  Han Jin Lee ,  Ju Eun Kim ,  Hyun Ah Kim ,  Jee Yeon Bae ,  Kwang Ho Na

发明人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  Sung Kwang Son ,  In Kyung Heo ,  Han Jin Lee ,  Ju Eun Kim ,  Hyun Ah Kim ,  Jee Yeon Bae ,  Kwang Ho Na

IPC分类号： C12N9/10 ,  C12P7/54 ,  C12P13/12

CPC分类号： C12N9/1085 ,  C12P7/54 ,  C12P13/12 ,  C12Y205/01049

摘要： The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.

摘要翻译： 本发明涉及具有O-乙酰高丝氨酸二硫氢化酶活性的新蛋白质，其突变蛋白质，编码该多核苷酸的多核苷酸，包含多核苷酸的重组载体，用重组载体转化的微生物，以及产生甲硫氨酸或乙酸的方法 使用蛋白质。 本发明的制备方法具有通过与现有方法相比具有更高的转化率和降低的反应时间来成本有效地生产L-甲硫氨酸和乙酸的优点，并且可以使使用突变体时添加的酶匀浆的量最小化 蛋白质，从而容易以高产率生产L-甲硫氨酸和乙酸。

公开(公告)号：US20130273615A1

公开(公告)日：2013-10-17

申请号：US13996990

申请日：2011-12-21

申请人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  In Kyung Heo ,  Ju Eun Kim ,  Hyun Ah Kim ,  Han Jin Lee ,  Kwang Ho Na ,  Sung Kwang Son

发明人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  In Kyung Heo ,  Ju Eun Kim ,  Hyun Ah Kim ,  Han Jin Lee ,  Kwang Ho Na ,  Sung Kwang Son

IPC分类号： C12N9/10

CPC分类号： C12N9/1029 ,  C12N1/00 ,  C12N15/70 ,  C12N15/74 ,  C12P13/06 ,  C12Y203/01031

摘要： The present invention relates to a polypeptide that is modified to have homoserine O-acetyltransferase activity, and in particular, the present invention provides a modified polypeptide having homoserine O-acetyltransferase activity, in which the amino acid at position 111 of a polypeptide having homoserine succinyltransferase activity is substituted with other amino acid.

摘要翻译： 本发明涉及被修饰为具有高丝氨酸O-乙酰转移酶活性的多肽，特别是本发明提供具有高丝氨酸O-乙酰转移酶活性的修饰多肽，其中具有高丝氨酸琥珀酰转移酶的多肽的111位氨基酸 活性被其他氨基酸取代。

公开(公告)号：US20130273614A1

公开(公告)日：2013-10-17

申请号：US13997054

申请日：2011-12-21

申请人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  Sung Kwang Son ,  In Kyung Heo ,  Han Jin Lee ,  Ju Eun Kim ,  Hyun Ah Kim ,  Jee Yeon Bae ,  Kwang Ho Na

发明人： So Young Kim ,  Yong Uk Shin ,  Chang Il Seo ,  Sung Kwang Son ,  In Kyung Heo ,  Han Jin Lee ,  Ju Eun Kim ,  Hyun Ah Kim ,  Jee Yeon Bae ,  Kwang Ho Na

IPC分类号： C12N9/10

CPC分类号： C12N9/1085 ,  C12P7/54 ,  C12P13/12 ,  C12Y205/01049

摘要： The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.

摘要翻译： 本发明涉及具有O-乙酰高丝氨酸二硫氢化酶活性的新蛋白质，其突变蛋白质，编码该多核苷酸的多核苷酸，包含多核苷酸的重组载体，用重组载体转化的微生物，以及产生甲硫氨酸或乙酸的方法 使用蛋白质。 本发明的制备方法具有通过与现有方法相比具有更高的转化率和降低的反应时间来成本有效地生产L-甲硫氨酸和乙酸的优点，并且可以使使用突变体时添加的酶匀浆的量最小化 蛋白质，从而容易以高产率生产L-甲硫氨酸和乙酸。