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    Plant seedling and embryo promoter
    2.
    发明授权
    Plant seedling and embryo promoter 有权
    植物幼苗和胚胎启动子

    公开(公告)号：US06376750B1

    公开(公告)日：2002-04-23

    申请号：US09575574

    申请日：2000-05-22

    Applicant: Su-May Yu ,  Yu-Chan Chao

    Inventor： Su-May Yu ,  Yu-Chan Chao

    IPC: A01H500

    CPC classification number: C12N15/8234 ,  C07K14/415

    Abstract: The invention relates to the promoter of a rice glycine-rich RNA binding protein gene and its use for expression of heterologous proteins in plant tissues, including tissues of the plant embryo and seedling.

    Abstract translation: 本发明涉及水稻富含甘氨酸的RNA结合蛋白基因的启动子及其在植物组织中表达异源蛋白质的用途，包括植物胚胎和幼苗的组织。

    Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability
    3.
    发明授权
    Use of green fluorescent protein to trace the infection of baculovirus in insects and to increase viral UV stability 失效
    使用绿色荧光蛋白来追踪昆虫中杆状病毒的感染并增加病毒的紫外线稳定性

    公开(公告)号：US5919445A

    公开(公告)日：1999-07-06

    申请号：US877564

    申请日：1997-06-17

    Applicant: Yu-Chan Chao

    Inventor： Yu-Chan Chao

    IPC: A01N63/00 ,  A01N63/02 ,  C07K14/435 ,  C12N15/00

    CPC classification number: C07K14/43595 ,  A01N63/00 ,  A01N63/02

    Abstract: Green fluorescent protein (GFP) gene was transplanted into baculovirus. The baculovirus was used to infect pests of vegetable crops. Three to five days after infection, the infected insects were found to emit strong fluorescence when exposed to a portable UV light. Therefore the spreading of baculovirus infection and disease progression can be effectively detected in the field. In addition, the GFP reduced UV damage to the baculovirus. As a result, GFP can increase the retention time of the virus as a pesticide in the field.

    Abstract translation: 将绿色荧光蛋白（GFP）基因移植到杆状病毒中。 杆状病毒用于感染蔬菜作物的害虫。 感染后三至五天，当被感染的昆虫暴露于便携式紫外光下时，被发现的昆虫发出强烈的荧光。 因此，可以有效地检测到杆状病毒感染和疾病进展的扩散。 此外，GFP降低了对杆状病毒的紫外线损伤。 因此，GFP可以增加现场作为农药的病毒的保留时间。

    PROTEIN EXPRESSION SYSTEMS
    7.
    发明申请
    PROTEIN EXPRESSION SYSTEMS 有权
    蛋白表达系统

    公开(公告)号：US20090068703A1

    公开(公告)日：2009-03-12

    申请号：US11851042

    申请日：2007-09-06

    Applicant: Yu-Chan Chao ,  Catherine Y.Y. Liu ,  Carol P. Y. Wu

    Inventor： Yu-Chan Chao ,  Catherine Y.Y. Liu ,  Carol P. Y. Wu

    IPC: C12P21/00

    CPC classification number: C12N15/85 ,  C12N2800/107 ,  C12N2830/00

    Abstract: Disclosed are recombinant non-insect host cells and use of activation of CMV or SV40 promoters by IE1, IE2 and the enhancer hr of the baculovirus in non-insect host cells for expression heterologous RNAs or polypeptides. Also disclosed are methods of using the cells.

    Abstract translation: 公开了重组非昆虫宿主细胞，并且使用IE6，IE2中的CMV或SV40启动子和非昆虫宿主细胞中杆状病毒的增强子hr用于表达异源RNA或多肽的活化。 还公开了使用细胞的方法。

    Hz-1 virus persistence-associated-gene 1 (PAG1) promoter uses therefor, and compositions containing same or products therefrom
    9.
    发明授权
    Hz-1 virus persistence-associated-gene 1 (PAG1) promoter uses therefor, and compositions containing same or products therefrom 失效
    Hz-1病毒持久性相关基因1（PAG1）启动子用于其中，以及含有其的组合物或来自其的产物

    公开(公告)号：US5911982A

    公开(公告)日：1999-06-15

    申请号：US634350

    申请日：1996-04-18

    Applicant: Yu-Chan Chao

    Inventor： Yu-Chan Chao

    IPC: C07K14/01 ,  A61K31/70 ,  C12N5/06 ,  C12N15/64 ,  C12P21/00

    CPC classification number: C07K14/005 ,  C12N15/86 ,  C12N2710/10022 ,  C12N2710/14143

    Abstract: Disclosed and claimed is the promoter, full-length or truncated, of the persistence-associated gene 1 (pag1) of Hz-1 virus, vectors containing the promoter, including with exogenous coding DNA, methods employing the vectors, compositions employing the vectors and products therefrom, and probes and primers for the promoter or functional fragment thereof. The promoter of pag1 gene is strong and if not comparable, it is better than the promoter activity demonstrated in the polyhedrin gene in insect cells. In addition, the pag1 promoter can be expressed more prominently as a short promoter. The pag1 promoter can also be expressed in transient transfected and permanently transfected cells. Further, the pag1 promoter can also be expressed when it is inserted into other viruses, such as Autographa californica nuclear polyhedrosis virus. The pag1 promoter has also been shown to express foreign genes strongly, such as in lacZ and luciferase. pag1 is driven by a constitutively expressed early type promoter, thus facilitating expression much earlier than the polyhedrin gene promoter.

    Abstract translation: 公开并要求保护的是Hz-1病毒的持久性相关基因1（pag1）的启动子，全长或截短的，包含启动子的载体，包括外源编码DNA，使用载体的方法，使用载体的组合物和 其产物，以及用于启动子或其功能片段的探针和引物。 pag1基因的启动子是强的，如果不能比较，它比在昆虫细胞中多角体蛋白基因中证明的启动子活性更好。 此外，pag1启动子可以作为短启动子更显着地表达。 pag1启动子也可以在瞬时转染和永久转染的细胞中表达。 此外，pag1启动子在插入其他病毒时也可以表达，例如苜蓿银纹夜蛾核型多角体病毒。 pag1启动子也被证明能强烈表达外源基因，如lacZ和萤光素酶。 pag1由组成型表达的早期型启动子驱动，因此比多角体蛋白基因启动子更早地表达。

    Prevention of viral infection by the induction of apoptosis and/or the use of an antiviral gene, hem1
    10.
    发明授权
    Prevention of viral infection by the induction of apoptosis and/or the use of an antiviral gene, hem1 失效
    通过诱导凋亡来预防病毒感染和/或使用抗病毒基因，hem1

    公开(公告)号：US5843736A

    公开(公告)日：1998-12-01

    申请号：US249617

    申请日：1994-05-26

    Applicant: Yu-Chan Chao

    Inventor： Yu-Chan Chao

    IPC: A61K38/00 ,  C07K14/01 ,  C12N15/113 ,  C12N15/00 ,  C12N5/00

    CPC classification number: C12N15/1131 ,  C07K14/005 ,  A61K38/00 ,  C12N2330/10 ,  C12N2710/14122

    Abstract: Disclosed is a new, effective and general methodology to prevent viral infection, which methodology is divided into two parts: 1) induction of the apoptosis (programmed cell death) of a host upon viral infection to block viral reproduction; and 2) the use of an apoptosis triggering gene, the persistency-associated gene 1 (pag1, now renamed as hem1 in light of the position thereof in the restriction map of the Hz-1 viral genome) to prevent the viral infection.

    Abstract translation: 披露了一种新的有效和一般的方法来预防病毒感染，该方法分为两部分：1）诱导宿主在病毒感染后的凋亡（程序性细胞死亡）以阻断病毒繁殖; 和2）使用凋亡触发基因，持续性相关基因1（pag1，现在根据其在Hz-1病毒基因组的限制性图谱中的位置改名为hem1）以防止病毒感染。

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