公开(公告)号：US07183403B2

公开(公告)日：2007-02-27

申请号：US11073560

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/04 ,  C12P21/06

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US07244569B2

公开(公告)日：2007-07-17

申请号：US10832299

申请日：2004-04-27

申请人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

发明人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

IPC分类号： C12N15/74 ,  C12N15/77 ,  C12N15/10 ,  C12N15/31 ,  C07H21/04 ,  C07K14/195 ,  C07K14/34

CPC分类号： C12N15/77

摘要： A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

摘要翻译： 可从热棒状杆菌分离的质粒，其包含编码具有SEQ ID NO：8所示氨基酸序列的Rep蛋白的基因或与SEQ ID NO所示的氨基酸序列具有90％以上同源性的氨基酸序列 ：8，并且具有约4.4kb或约6kb的大小，或其衍生物。

公开(公告)号：US06905819B1

公开(公告)日：2005-06-14

申请号：US09636458

申请日：2000-08-11

申请人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

发明人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

IPC分类号： C12N15/00 ,  C12N1/21 ,  C12N15/77 ,  C12R1/15 ,  C12N15/10 ,  C12N1/20 ,  C12Q1/68

CPC分类号： C12N15/77

摘要： A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

摘要翻译： 能够从嗜热棒杆菌分离的质粒，其包含编码具有SEQ ID NO：4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90％以上的氨基酸序列 SEQ ID NO：4所示的序列，其大小为约4.4kb或约6kb，或其衍生物。

公开(公告)号：US20050239176A1

公开(公告)日：2005-10-27

申请号：US11073560

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07K14/34 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/26 ,  C12N9/88 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12N15/56 ,  C12N15/60 ,  C12P13/04 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74 ,  C12P13/08

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US20050176127A1

公开(公告)日：2005-08-11

申请号：US11073550

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07K14/34 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/26 ,  C12N9/88 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12N15/56 ,  C12N15/60 ,  C12N1/21 ,  C12N15/74 ,  C12P13/08

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

公开(公告)号：US07125977B2

公开(公告)日：2006-10-24

申请号：US11073550

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/04

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US06995250B1

公开(公告)日：2006-02-07

申请号：US10089057

申请日：2000-10-04

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12P21/06

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US06338956B1

公开(公告)日：2002-01-15

申请号：US09634632

申请日：2000-08-08

申请人： Eiichiro Kimura ,  Yoshimi Kikuchi ,  Yoshio Kawahara ,  Shinya Goto ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu

发明人： Eiichiro Kimura ,  Yoshimi Kikuchi ,  Yoshio Kawahara ,  Shinya Goto ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu

IPC分类号： C12P1304

CPC分类号： C12P13/222 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

摘要翻译： 利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸，使发酵产物在培养基中产生和积累，收集发酵产物，其中所用的微生物通过引入进行修饰 编码热休克蛋白的基因中的至少一种和编码特异性用于热休克蛋白基因的西格玛因子的基因以增强细胞中的热休克蛋白的表达量，由此允许微生物添加 否则会抑制微生物的生长和/或发酵产物的产生。

公开(公告)号：US5977331A

公开(公告)日：1999-11-02

申请号：US750152

申请日：1996-12-12

申请人： Yoko Asakura ,  Yoshihiro Usuda ,  Nobuharu Tsujimoto ,  Eiichiro Kimura ,  Chizu Abe ,  Yoshio Kawahara ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

发明人： Yoko Asakura ,  Yoshihiro Usuda ,  Nobuharu Tsujimoto ,  Eiichiro Kimura ,  Chizu Abe ,  Yoshio Kawahara ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12P13/08 ,  C12P13/14 ,  C07H21/02 ,  C12N1/20 ,  C12P13/04

CPC分类号： C12N9/0008 ,  C12P13/08 ,  C12P13/14

摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.

摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号 日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因 谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。

公开(公告)号：US6156532A

公开(公告)日：2000-12-05

申请号：US894223

申请日：1997-08-25

申请人： Eiichiro Kimura ,  Yoshimi Kikuchi ,  Yoshio Kawahara ,  Shinya Goto ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu

发明人： Eiichiro Kimura ,  Yoshimi Kikuchi ,  Yoshio Kawahara ,  Shinya Goto ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12P13/14 ,  C12P13/22 ,  C12P1/00

CPC分类号： C12P13/222 ,  C12P13/04 ,  C12P13/08 ,  C12P13/14

摘要： A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a .sigma. factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

摘要翻译： PCT No.PCT / JP96 / 00287 Sec。 371日期1997年8月25日第 102（e）日期1997年8月25日PCT提交1996年2月9日PCT公布。 公开号WO96 / 26289 日期1996年8月29日利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸，以使发酵产物在培养基中产生并积累，并收集发酵产物，其中微生物为 通过引入编码热休克蛋白的基因和编码特异性用于热休克蛋白基因的σ因子的基因中的至少一种来修饰，以增强细胞中热休克蛋白的表达量，由此微生物 被允许增加抗应力，否则会抑制微生物的生长和/或发酵产物的产生。