公开(公告)号：US07183403B2

公开(公告)日：2007-02-27

申请号：US11073560

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/04 ,  C12P21/06

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US07244569B2

公开(公告)日：2007-07-17

申请号：US10832299

申请日：2004-04-27

申请人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

发明人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

IPC分类号： C12N15/74 ,  C12N15/77 ,  C12N15/10 ,  C12N15/31 ,  C07H21/04 ,  C07K14/195 ,  C07K14/34

CPC分类号： C12N15/77

摘要： A plasmid isolatable from Corynebacterium thermoaminogenes, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

摘要翻译： 可从热棒状杆菌分离的质粒，其包含编码具有SEQ ID NO：8所示氨基酸序列的Rep蛋白的基因或与SEQ ID NO所示的氨基酸序列具有90％以上同源性的氨基酸序列 ：8，并且具有约4.4kb或约6kb的大小，或其衍生物。

公开(公告)号：US06905819B1

公开(公告)日：2005-06-14

申请号：US09636458

申请日：2000-08-11

申请人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

发明人： Yumi Matsuzaki ,  Eiichiro Kimura ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi ,  Yoshio Kawahara ,  Shinichi Sugimoto

IPC分类号： C12N15/00 ,  C12N1/21 ,  C12N15/77 ,  C12R1/15 ,  C12N15/10 ,  C12N1/20 ,  C12Q1/68

CPC分类号： C12N15/77

摘要： A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

摘要翻译： 能够从嗜热棒杆菌分离的质粒，其包含编码具有SEQ ID NO：4所示的氨基酸序列的Rep蛋白的基因或与氨基酸同源性为90％以上的氨基酸序列 SEQ ID NO：4所示的序列，其大小为约4.4kb或约6kb，或其衍生物。

公开(公告)号：US20050239176A1

公开(公告)日：2005-10-27

申请号：US11073560

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07K14/34 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/26 ,  C12N9/88 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12N15/56 ,  C12N15/60 ,  C12P13/04 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74 ,  C12P13/08

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US20050176127A1

公开(公告)日：2005-08-11

申请号：US11073550

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07K14/34 ,  C12N9/02 ,  C12N9/04 ,  C12N9/12 ,  C12N9/26 ,  C12N9/88 ,  C12N15/31 ,  C12N15/53 ,  C12N15/54 ,  C12N15/56 ,  C12N15/60 ,  C12N1/21 ,  C12N15/74 ,  C12P13/08

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

公开(公告)号：US07125977B2

公开(公告)日：2006-10-24

申请号：US11073550

申请日：2005-03-08

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/04

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US06995250B1

公开(公告)日：2006-02-07

申请号：US10089057

申请日：2000-10-04

申请人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

发明人： Seiko Hirano ,  Eiichiro Kimura ,  Tsuyoshi Osumi ,  Kazuhiko Matsui ,  Yoshio Kawahara ,  Gen Nonaka ,  Yumi Matsuzaki ,  Naoki Akiyoshi ,  Kanae Nakamura ,  Osamu Kurahashi ,  Tsuyoshi Nakamatsu ,  Shinichi Sugimoto

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12P21/06

CPC分类号： C12Y108/01004 ,  C07K14/34 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1205 ,  C12N9/2408 ,  C12N9/88 ,  C12Y101/01041 ,  C12Y102/01051 ,  C12Y102/04002 ,  C12Y104/01004 ,  C12Y203/03001 ,  C12Y207/01011 ,  C12Y302/01026 ,  C12Y401/01031 ,  C12Y401/03001 ,  C12Y402/01003 ,  C12Y604/01001 ,  C12Y604/01002

摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.

摘要翻译： 基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为 与谷氨酸棒杆菌相比在更高的温度下起作用的酶。 通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。 使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

公开(公告)号：US20110053183A1

公开(公告)日：2011-03-03

申请号：US12676827

申请日：2008-09-08

申请人： Yumi Matsuzaki ,  Yo Mabuchi ,  Satoru Morikawa ,  Hideyuki Okano

发明人： Yumi Matsuzaki ,  Yo Mabuchi ,  Satoru Morikawa ,  Hideyuki Okano

IPC分类号： G01N33/53 ,  C12N5/077 ,  C12N5/078

CPC分类号： C12N5/0663 ,  C12N5/0665 ,  C12N2509/00

摘要： The present invention is intended to provide methods for highly enriching human mesenchymal stem cells from a cell population containing the human mesenchymal stem cells. To highly enrich human mesenchymal stem cells, CD271+CD90+ cells are recovered by using flow cytometry etc. from a cell population containing the human mesenchymal stem cells. If the cell population contains blood cells (as in the case of a cell population prepared from a bone marrow, a peripheral blood etc.), CD45−CD235a−CD271+CD90+ cells are recovered. These cell fractions contain with high purity the mesenchymal stem cells having self-renewal capability, self-replicating capability and pluripotency. Therefore, human mesenchymal stem cells can be highly enriched by recovering CD271+CD90+ cells from the cell population containing the human mesenchymal stem cells.

摘要翻译： 本发明旨在提供从含有人间充质干细胞的细胞群中高度富集人类间充质干细胞的方法。 为了高度富集人间充质干细胞，通过使用流式细胞仪等从含有人间充质干细胞的细胞群体中回收CD271 + CD90 +细胞。 如果细胞群含有血细胞（如在从骨髓，外周血等制备的细胞群的情况下），则回收CD45-CD235a-CD271 + CD90 +细胞。 这些细胞部分含有高纯度的具有自我更新能力，自我复制能力和多能性的间充质干细胞。 因此，通过从含有人间充质干细胞的细胞群中回收CD271 + CD90 +细胞，可以高度富集人间充质干细胞。

公开(公告)号：US20050014236A1

公开(公告)日：2005-01-20

申请号：US10790224

申请日：2004-03-02

申请人： Yumi Matsuzaki ,  Jun Nakamura ,  Kenichi Hashiguchi

发明人： Yumi Matsuzaki ,  Jun Nakamura ,  Kenichi Hashiguchi

IPC分类号： C12N1/20 ,  C12N1/21 ,  C12P13/08 ,  C12P13/10 ,  C12N9/10

CPC分类号： C12P13/10 ,  C12P13/08

摘要： L-arginine or L-lysine is produced by culturing a coryneform bacterium having an L-arginine- or L-lysine-producing ability and modified so that glutamine synthetase activity is enhanced, e.g., a coryneform bacterium which is modified so that adenylylation of glutamine synthetase is eliminated. L-arginine or L-lysine are produced by culturing the bacterium in a medium and allowing L-arginine or L-lysine to accumulate in the medium, and collecting the L-arginine or L-lysine from the medium.

摘要翻译： 通过培养具有L-精氨酸或L-赖氨酸生产能力的棒状细菌产生L-精氨酸或L-赖氨酸，并对其进行了修饰，使得谷氨酰胺合成酶活性得到增强，例如，修饰为使得谷氨酰胺腺苷酰化的棒状细菌 合成酶被消除。 通过在培养基中培养细菌并使L-精氨酸或L-赖氨酸在培养基中积聚并从培养基中收集L-精氨酸或L-赖氨酸来产生L-精氨酸或L-赖氨酸。