公开(公告)号：US20070124826A1

公开(公告)日：2007-05-31

申请号：US10568502

申请日：2004-08-24

申请人： Yutaka Hanazono ,  Kyoko Sasaki

发明人： Yutaka Hanazono ,  Kyoko Sasaki

IPC分类号： A01K67/027 ,  C12N5/08

CPC分类号： A01K67/0271 ,  A01K2227/103 ,  A01K2227/106 ,  A61K35/12

摘要： A method of differentiation from an embryonic stem cell of a primate into a hematopoietic cell, comprising maintaining an embryonic stem cell of a primate under conditions suitable for induction of differentiation into a hematopoietic cell, transplanting the resulting cell into a fetus in a uterus of a pregnant sheep, rearing the fetus, administering a cytokine specific for a primate to a born lamb, and obtaining a hematopoietic cell of a primate from a sheep obtained by rearing the lamb, a method for producing a hematopoietic cell of a primate, a hematopoietic cell obtained by the method, and a method for producing a chimeric sheep which produces a hematopoietic cell of a primate, comprising maintaining an embryonic stem cell of a primate under conditions suitable for induction of differentiation into a hematopoietic cell, and transplanting the resulting cell into a fetus in a uterus of a pregnant sheep. According to the present invention, it becomes possible to develop a means for treating a disease or a condition requiring construction of a hematopoietic system or an action of a hematopoietic cell.

摘要翻译： 一种从灵长类动物的胚胎干细胞分化成造血细胞的方法，包括在适于诱导分化成造血细胞的条件下保持灵长类动物的胚胎干细胞，将所得细胞移植到子宫内的胎儿中 怀孕的绵羊，养育胎儿，向生长的羔羊施用特异性的灵长类动物的细胞因子，以及从通过饲养羊羔获得的绵羊获得灵长类动物的造血细胞，产生灵长类动物的造血细胞的方法，造血细胞 通过该方法获得的嵌合绵羊的制备方法以及产生灵长类动物造血细胞的嵌合羊的方法，包括在适于诱导分化成造血细胞的条件下保持灵长类动物的胚胎干细胞，并将得到的细胞移植到 胎儿在​​怀孕的绵羊的子宫。 根据本发明，可以开发用于治疗需要构建造血系统或造血细胞的作用的疾病或病症的手段。

公开(公告)号：US07323337B2

公开(公告)日：2008-01-29

申请号：US10479912

申请日：2002-05-29

申请人： Yutaka Hanazono ,  Yasuji Ueda ,  Yasushi Kondo ,  Yutaka Suzuki

发明人： Yutaka Hanazono ,  Yasuji Ueda ,  Yasushi Kondo ,  Yutaka Suzuki

IPC分类号： C12N15/86 ,  C12N5/00 ,  C12N15/82 ,  C12N5/02

CPC分类号： G01N33/5073 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2227/106 ,  A01K2267/025 ,  A01K2267/03 ,  A61K48/00 ,  C12N15/8509 ,  C12N15/86 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2810/6081 ,  G01N33/5008 ,  G01N2333/22

摘要： Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.

摘要翻译： 通过使用作为水泡性口炎病毒（VSV）的表面糖蛋白的VSV-G蛋白假型的猿免疫缺陷病毒载体（SIV），已经成功地实现了将高效率的基因转移到灵长类衍生的胚胎干细胞（ES）细胞中。本发明 提供猿猴免疫缺陷病毒载体，用于基因转移到灵长类动物ES细胞。 使用本发明的载体将基因转移到灵长类动物ES细胞的方法可用于例如研究胚胎学和疾病，临床应用和灵长类动物的实验模型。 该方法还可用于测定和筛选能够增强组织或细胞的特异性分化的基因和试剂，并且其可用于制备从ES细胞分化的所需细胞或组织。

公开(公告)号：US07238777B2

公开(公告)日：2007-07-03

申请号：US10270555

申请日：2002-10-16

申请人： Akira Itoh ,  Yutaka Hanazono ,  Takashi Okada ,  Keiya Ozawa

发明人： Akira Itoh ,  Yutaka Hanazono ,  Takashi Okada ,  Keiya Ozawa

IPC分类号： C07K1/00

CPC分类号： C07K16/2896 ,  C07K2317/622 ,  C07K2319/00 ,  C12N2799/022

摘要： The object of the present invention is to provide an agent for specific adsorption of type 5 adenoviral vector to undifferentiated blood cells which enables infection with a viral concentration that does not impart toxicity to undifferentiated blood cells, without requiring the construction of a new viral genome, or special modification of viral particles such as biotination, etc. The above objective has been achieved by providing a polypeptide which has affinity for both adenovirus and undifferentiated blood cells. Use of the polypeptide of the present invention enables improved efficiency of adenoviral vector-mediated gene transfer of any gene to undifferentiated blood cells, and there is provided a method of introducing any gene for which transient expression in these cells is desired. In particular, the method is useful for intracellular DNA recombination by transiently expressing site-specific recombinase, and for induction into any cells of different lines by transient expression of master genes.

摘要翻译： 本发明的目的是提供一种用于特异性吸附5型腺病毒载体的未分化血细胞的试剂，其能够以不对未分化血细胞赋予毒性的病毒浓度进行感染，而不需要构建新的病毒基因组， 或特异性修饰病毒颗粒如生物素化等。上述目的已通过提供对腺病毒和未分化血细胞具有亲和力的多肽来实现。 使用本发明的多肽能够提高任何基因向未分化血细胞的腺病毒载体介导的基因转移的效率，并且提供了引入其中期望这些细胞中瞬时表达的任何基因的方法。 特别地，该方法对于通过瞬时表达位点特异性重组酶的细胞内DNA重组以及通过主基因的瞬时表达诱导到不同细胞系的任何细胞中是有用的。

公开(公告)号：US06995011B2

公开(公告)日：2006-02-07

申请号：US10188075

申请日：2002-07-03

申请人： Akira Itoh ,  Yutaka Hanazono ,  Keiya Ozawa

发明人： Akira Itoh ,  Yutaka Hanazono ,  Keiya Ozawa

IPC分类号： C12N15/09 ,  C12N5/10

CPC分类号： C12N15/90 ,  A01K2217/05 ,  A01K2217/075 ,  A61K48/00 ,  C12N2800/30 ,  C12N2830/003 ,  C12N2830/205 ,  C12N2840/20 ,  C12N2840/203

摘要： Disclosed is an expression control system with which any gene of interest can be transferred into mammalian cells to thereby change the nature thereof and the gene can be excised at any time by administering a low molecular weight compound to the cells. Specifically disclosed is a vector satisfying specific requirements when inserted into the genome of a mammalian cell.

摘要翻译： 公开了一种表达控制系统，其中任何感兴趣的基因可以转移到哺乳动物细胞中，从而改变其性质，并且可以在任何时候通过向细胞施用低分子量化合物来切除该基因。 具体公开的是当插入到哺乳动物细胞的基因组中时满足特定要求的载体。

公开(公告)号：US20110262959A1

公开(公告)日：2011-10-27

申请号：US13142222

申请日：2009-06-30

申请人： Eiji Kobayashi ,  Takashi Yokoo ,  Yutaka Hanazono ,  Hiroshi Nagashima

发明人： Eiji Kobayashi ,  Takashi Yokoo ,  Yutaka Hanazono ,  Hiroshi Nagashima

IPC分类号： C12Q1/02

CPC分类号： A01K67/0271 ,  A01K2227/105 ,  A01K2227/106 ,  A01K2227/108 ,  A01K2267/03 ,  C12N15/873 ,  G01N33/5005

摘要： The present invention provides a method of evaluating the possibility of a stem cell being able to differentiate into a cell that constitutes a desired tissue in a living organism, comprising the following steps of: (1) transplanting a stem cell to be evaluated into a primordium of a desired tissue of a non-human mammal, (2) culturing the tissue primordium in vitro, and (3) determining the possibility that the stem cell differentiates into a cell that constitutes the tissue in a living organism, with the degree of the dispersion of cells derived from the transplanted stem cell in the cultured tissue primordium as an index.

摘要翻译： 本发明提供一种评估干细胞能够分化为构成生物体中所需组织的细胞的可能性的方法，包括以下步骤：（1）将要评估的干细胞移植到原基中 的非人哺乳动物的期望组织，（2）在体外培养组织原基，和（3）确定干细胞分化为构成活生物体组织的细胞的可能性，其程度为 作为指标，将来自移植干细胞的细胞分散在培养的组织原基中。

公开(公告)号：US20060257381A1

公开(公告)日：2006-11-16

申请号：US10562322

申请日：2004-06-25

申请人： Keiya Ozawa ,  Yutaka Hanazono ,  Kyoji Ueda ,  Yasuji Ueda ,  Mamoru Hasegawa

发明人： Keiya Ozawa ,  Yutaka Hanazono ,  Kyoji Ueda ,  Yasuji Ueda ,  Mamoru Hasegawa

IPC分类号： A61K48/00 ,  C12N5/08

CPC分类号： A01K67/0271 ,  A61K48/0066 ,  A61K2035/124 ,  C07K14/505 ,  C07K14/715 ,  C07K14/7153 ,  C07K2319/00 ,  C12N5/0647 ,  C12N2799/027

摘要： The present invention provides a method for transplanting lymphohematopoietic cells into a mammal, which comprises the step of injecting cells into a bone marrow cavity, and wherein the cells have an exogenous gene encoding a receptor that induces cell proliferation in response to ligand binding. By combining intra-bone marrow transplantation (iBMT) and selective amplifier gene (SAG), marrow conditioning before the injection of the cells can be omitted. The present invention further provides a bone marrow transplant and a kit for transplanting lymphohematopoietic cells into mammals. Furthermore, the invention provides an SAG particularly suitable for such transplantation.

摘要翻译： 本发明提供了将淋巴细胞生成细胞移植到哺乳动物中的方法，其包括将细胞注入骨髓腔中的步骤，并且其中细胞具有编码受体的外源基因，所述受体诱导响应于配体结合的细胞增殖。 通过组合骨髓移植（iBMT）和选择性放大基因（SAG），可以省略注射细胞前的骨髓调节。 本发明进一步提供骨髓移植和用于将淋巴造血细胞移植到哺乳动物中的试剂盒。 此外，本发明提供了特别适合于这种移植的SAG。