公开(公告)号：US20110177547A1

公开(公告)日：2011-07-21

申请号：US13007176

申请日：2011-01-14

申请人： Zheng Xia ,  Tania Chakrabarty ,  Haojun Fu ,  Dan Mueth

发明人： Zheng Xia ,  Tania Chakrabarty ,  Haojun Fu ,  Dan Mueth

IPC分类号： C12Q1/04 ,  G01N1/20 ,  H05H3/00 ,  G02B1/04 ,  B29D11/00

CPC分类号： G01N1/34 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/0655 ,  G01N15/1463 ,  G01N2001/007 ,  G01N2015/149 ,  G02B5/32 ,  G03H2001/0077

摘要： A fluidic device includes an arrangement of channels for introducing a sample containing particles of interest into a processing chamber. The chamber is in fluid communication with collecting channels via low-flow connection channels. Particles in the sample may be observed and diverted from the processing chamber by application of a motive force such as optical trapping into a collection channel. Once in the collection channel, particles can be collected, including by trapping in a porous matrix.

摘要翻译： 流体装置包括用于将含有感兴趣的颗粒的样品引入处理室的通道布置。 室通过低流量连接通道与收集通道流体连通。 可以通过将诸如光学捕获的动力施加到收集通道中来观察和转移来自处理室的颗粒。 一旦在收集通道中，可以收集颗粒，包括通过捕集在多孔基质中。

公开(公告)号：US20110026009A1

公开(公告)日：2011-02-03

申请号：US12440949

申请日：2007-09-14

申请人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

发明人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

IPC分类号： G01N33/48 ,  G01B11/30

CPC分类号： G01N33/48 ,  G01N33/54373 ,  G01N33/80

摘要： Methods and apparatus for analyzing surface properties of particles are provided. A method for analyzing the surface properties of the particle includes a associating a first particle with a first capture zone having a specific binding affinity for a first chemical species, applying an optical force to the first particle, sensing a response of the first particle to the optical force, and using the sensed response to determine the presence, absence or quantity of the first chemical species on the first particle surface. This process may be repeated in parallel to test multiple particles. In addition to directly testing the surface properties of the particles, the method can be used in direct, indirect and competitive assays to determine the presence, absence or quantity of free or immobilized analytes. A fluidic cartridge with capture zones having avidities that are tuned for the use of optical forces is provided. A software routine for performing the method is also provided.

摘要翻译： 提供了分析颗粒表面性质的方法和装置。 用于分析颗粒的表面性质的方法包括将第一颗粒与对第一化学物质具有特异性结合亲和力的第一捕获区缔合，向第一颗粒施加光学力，感测第一颗粒对第一颗粒的响应 并且使用感测到的响应来确定第一颗粒表面上的第一化学物质的存在，不存在或数量。 该过程可以并行重复以测试多个颗粒。 除了直接测试颗粒的表面性质之外，该方法可用于直接，间接和竞争性测定，以确定游离或固定化分析物的存在，不存在或数量。 提供了一种具有捕获区域的流体盒，其具有被调整以用于光学力的空间。 还提供了用于执行该方法的软件程序。

公开(公告)号：US08169600B2

公开(公告)日：2012-05-01

申请号：US12440949

申请日：2007-09-14

申请人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

发明人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

IPC分类号： G01N33/48

CPC分类号： G01N33/48 ,  G01N33/54373 ,  G01N33/80

摘要： Methods and apparatus for analyzing surface properties of particles are provided. A method for analyzing the surface properties of the particle includes a associating a first particle with a first capture zone having a specific binding affinity for a first chemical species, applying an optical force to the first particle, sensing a response of the first particle to the optical force, and using the sensed response to determine the presence, absence or quantity of the first chemical species on the first particle surface. This process may be repeated in parallel to test multiple particles. In addition to directly testing the surface properties of the particles, the method can be used in direct, indirect and competitive assays to determine the presence, absence or quantity of free or immobilized analytes. A fluidic cartridge with capture zones having avidities that are tuned for the use of optical forces is provided. A software routine for performing the method is also provided.

摘要翻译： 提供了分析颗粒表面性质的方法和装置。 用于分析颗粒的表面性质的方法包括将第一颗粒与对第一化学物质具有特异性结合亲和力的第一捕获区缔合，向第一颗粒施加光学力，感测第一颗粒对第一颗粒的响应 并且使用感测到的响应来确定第一颗粒表面上的第一化学物质的存在，不存在或数量。 该过程可以并行重复以测试多个颗粒。 除了直接测试颗粒的表面性质之外，该方法可用于直接，间接和竞争性测定，以确定游离或固定化分析物的存在，不存在或数量。 提供了一种具有捕获区域的流体盒，其具有被调整以用于光学力的空间。 还提供了用于执行该方法的软件程序。

公开(公告)号：US20090075826A1

公开(公告)日：2009-03-19

申请号：US12232165

申请日：2008-09-11

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C40B20/08 ,  C40B60/10

CPC分类号： B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0454 ,  G01N35/00029 ,  G01N2001/007

摘要： The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis.

摘要翻译： 本发明涉及一种用于对物体进行排序并识别取证样本中的物体的装置和方法，包括使用全息光学捕集对来自污染物的物体进行分类，以及对对象执行（单细胞）基于PCR的STR分析以确定其识别 。 另外，作为用于分类对象的支持的芯片也可以用于进行基于单细胞PCR的STR分析。 在另一个实施方案中，在进行基于单细胞PCR的STR分析之前，使用微流体芯片来流式化样品并对对象进行分类。 在不存在或存在微流体流和分选的情况下用于使用HOT分选的芯片也可以与用于单细胞基于PCR的STR分析相同。

公开(公告)号：US07998676B2

公开(公告)日：2011-08-16

申请号：US12232165

申请日：2008-09-11

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C12Q1/68

CPC分类号： B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0454 ,  G01N35/00029 ,  G01N2001/007

摘要： The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis.

摘要翻译： 本发明涉及一种用于对物体进行排序并识别取证样本中的物体的装置和方法，包括使用全息光学捕集对来自污染物的物体进行分类，以及对对象执行（单细胞）基于PCR的STR分析以确定其识别 。 另外，作为用于分类对象的支持的芯片也可以用于进行基于单细胞PCR的STR分析。 在另一个实施方案中，在进行基于单细胞PCR的STR分析之前，使用微流体芯片来流式化样品并对对象进行分类。 在不存在或存在微流体流和分选的情况下用于使用HOT分选的芯片也可以与用于单细胞基于PCR的STR分析相同。

公开(公告)号：US20120028848A1

公开(公告)日：2012-02-02

申请号：US13200940

申请日：2011-10-05

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C12M1/40 ,  C40B60/12

CPC分类号： G01N33/5091 ,  G01N33/56966 ,  G01N2800/367

摘要： The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody.

摘要翻译： 本发明涉及一种排序物品的方法和装置，包括：提供具有所需物品和不需要物品的样品; 用抗体涂覆样品架的表面; 将洗脱样品置于样品架上; 将所需物体中的抗原与样品架表面上的抗体结合，将物体分类成有用物体和不需要的物体; 分离所需物体; 并对所需物体进行基于PCR的STR分析。 在一个实施例中，全息光学捕获用于进一步分类所需物体。 在其他实施方案中，所需物体是精子，抗体是人精子特异性抗体，PCR是基于单细胞PCR的STR分析。 在其它实施方案中，结合是直接或间接的，配体用于结合对象特异性有机分子，并且蛋白A或蛋白G用于结合抗体。

公开(公告)号：US08067170B2

公开(公告)日：2011-11-29

申请号：US12232164

申请日：2008-09-11

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C12Q1/68

CPC分类号： G01N33/5091 ,  G01N33/56966 ,  G01N2800/367

摘要： The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody.

摘要翻译： 本发明涉及一种排序物品的方法和装置，包括：提供具有所需物品和不需要物品的样品; 用抗体涂覆样品架的表面; 将洗脱样品置于样品架上; 将所需物体中的抗原与样品架表面上的抗体结合，将物体分类成有用物体和不需要的物体; 分离所需物体; 并对所需物体进行基于PCR的STR分析。 在一个实施例中，全息光学捕获用于进一步分类所需物体。 在其他实施方案中，所需物体是精子，抗体是人精子特异性抗体，PCR是基于单细胞PCR的STR分析。 在其它实施方案中，结合是直接或间接的，配体用于结合对象特异性有机分子，并且蛋白A或蛋白G用于结合抗体。

公开(公告)号：US20110223653A1

公开(公告)日：2011-09-15

申请号：US13067226

申请日：2011-05-18

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C12M1/34

CPC分类号： B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0454 ,  G01N35/00029 ,  G01N2001/007

摘要： The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis.

摘要翻译： 本发明涉及一种用于对物体进行排序并识别取证样本中的物体的装置和方法，包括使用全息光学捕集对来自污染物的物体进行分类，以及对对象执行（单细胞）基于PCR的STR分析以确定其识别 。 另外，作为用于分类对象的支持的芯片也可以用于进行基于单细胞PCR的STR分析。 在另一个实施方案中，在进行基于单细胞PCR的STR分析之前，使用微流体芯片来流式化样品并对对象进行分类。 在不存在或存在微流体流和分选的情况下用于使用HOT分选的芯片也可以与用于单细胞基于PCR的STR分析相同。

公开(公告)号：US20090068671A1

公开(公告)日：2009-03-12

申请号：US12232164

申请日：2008-09-11

申请人： Tania Chakrabarty

发明人： Tania Chakrabarty

IPC分类号： C12Q1/68 ,  C12M1/00

CPC分类号： G01N33/5091 ,  G01N33/56966 ,  G01N2800/367

摘要： The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody.

摘要翻译： 本发明涉及一种排序物品的方法和装置，包括：提供具有所需物品和不需要物品的样品; 用抗体涂覆样品架的表面; 将洗脱样品置于样品架上; 将所需物体中的抗原与样品架表面上的抗体结合，将物体分类成有用物体和不需要的物体; 分离所需物体; 并对所需物体进行基于PCR的STR分析。 在一个实施例中，全息光学捕获用于进一步分类所需物体。 在其他实施方案中，所需物体是精子，抗体是人精子特异性抗体，PCR是基于单细胞PCR的STR分析。 在其它实施方案中，结合是直接或间接的，配体用于结合对象特异性有机分子，并且蛋白A或蛋白G用于结合抗体。