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公开(公告)号：US20070006335A1
公开(公告)日：2007-01-04
申请号：US11058689
申请日：2005-02-14
申请人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
发明人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C12N5/04 , C07K14/415
CPC分类号： C12N15/8216
摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.
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2.发明申请公开(公告)号：US20090181851A1
公开(公告)日：2009-07-16
申请号：US12343229
申请日：2008-12-23
申请人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward A. Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
发明人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward A. Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
CPC分类号： C12N15/8216
摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.
摘要翻译： 本发明涉及启动子序列和启动子控制元件，包含启动子和控制元件的多核苷酸构建体，以及鉴定启动子，控制元件或其片段的方法。 本发明还涉及本发明启动子或启动子控制元件调节转录水平的用途。
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公开(公告)号：US20110016587A1
公开(公告)日：2011-01-20
申请号：US12698945
申请日：2010-02-02
申请人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward A. Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
发明人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward A. Kiegle , Shing Kwok , Yu-Ping Lu , Leonard Medrano , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
CPC分类号： C12N15/8216
摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.
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公开(公告)号：US20060021083A1
公开(公告)日：2006-01-26
申请号：US11097589
申请日：2005-04-01
申请人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward Kiegle , Shing Kwok , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
发明人： Zhihong Cook , Yiwen Fang , Kenneth Feldmann , Edward Kiegle , Shing Kwok , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane Jofuku , Jonathan Donson
CPC分类号： C12N15/8222
摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.
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5.发明申请公开(公告)号：US20100037346A1
公开(公告)日：2010-02-11
申请号：US12575402
申请日：2009-10-07
申请人： Zhihong COOK , Yiwen Fang , Kenneth A. Feldmann , Edward A. Kiegle , Shing Kwok , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane K. Jofuku , Jonathan Donson , Leonard Medrano
发明人： Zhihong COOK , Yiwen Fang , Kenneth A. Feldmann , Edward A. Kiegle , Shing Kwok , Roger Pennell , Richard Schneeberger , Chuan-Yin Wu , Nestor Apuya , Diane K. Jofuku , Jonathan Donson , Leonard Medrano
CPC分类号： C12N15/8222
摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.
摘要翻译： 本发明涉及启动子序列和启动子控制元件，包含启动子和控制元件的多核苷酸构建体，以及鉴定启动子，控制元件或其片段的方法。 本发明还涉及本发明启动子或启动子控制元件调节转录水平的用途。
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6.发明申请公开(公告)号：US20060015970A1
公开(公告)日：2006-01-19
申请号：US11010239
申请日：2004-12-09
申请人： Roger Pennell , Jack Okamuro , Richard Schneeberger , Yiwen Fang , Shing Kwok , Diane Jofuku , Edward Kiegle , Jonathan Donson , Nestor Apuya
发明人： Roger Pennell , Jack Okamuro , Richard Schneeberger , Yiwen Fang , Shing Kwok , Diane Jofuku , Edward Kiegle , Jonathan Donson , Nestor Apuya
IPC分类号： A01H1/00 , C12N15/82 , C07H21/04 , C07K14/415 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8241
摘要： Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants.
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公开(公告)号：US06479291B2
公开(公告)日：2002-11-12
申请号：US09265576
申请日：1999-03-09
IPC分类号： C12N1582
CPC分类号： C12N15/1137 , C07K14/005 , C07K14/415 , C07K14/70571 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/1085 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36143 , C12N2770/36162 , C12N2799/021 , C12N2810/609 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , G01N2333/4353
摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.
摘要翻译： 本发明提供了用于在真核细胞的细胞质中表达抑制性RNA的新型遗传构建体。 抑制性RNA可以是反义RNA或共抑制RNA，并且起减少目标细胞中目的基因表达的作用。 本发明的遗传结构能够在真核细胞的细胞质中复制并且包含与编码多核苷酸的功能性组合的启动子区。 遗传结构可以设计成在植物细胞，酵母细胞和哺乳动物细胞的细胞质中复制。 当感兴趣的真核细胞是植物细胞时，遗传构建优选衍生自植物RNA病毒。 植物RNA病毒衍生的遗传结构可以使用植物病毒亚基因组启动子，包括与RNA编码区域功能组合的来自烟草花叶病毒的亚基因组启动子。 在本发明的一个优选实施方案中，诱导植物细胞产生升高水平的类胡萝卜素八氢番茄红素。 通过使用本发明的载体抑制酶八氢番茄红素去饱和酶的表达来实现提高的八氢番茄聚糖水平。
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公开(公告)号：US06720183B2
公开(公告)日：2004-04-13
申请号：US10103450
申请日：2002-03-20
IPC分类号： C12N1583
CPC分类号： C12N15/1137 , C07K14/005 , C07K14/415 , C07K14/70571 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/1085 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36143 , C12N2770/36162 , C12N2799/021 , C12N2810/609 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , G01N2333/4353
摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.
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公开(公告)号：US06376752B1
公开(公告)日：2002-04-23
申请号：US09436068
申请日：1999-11-08
IPC分类号： A01H500
CPC分类号： C12N15/1137 , C07K14/005 , C07K14/415 , C07K14/70571 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/1085 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36143 , C12N2770/36162 , C12N2799/021 , C12N2810/609 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , G01N2333/4353
摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.
摘要翻译： 本发明提供了用于在真核细胞的细胞质中表达抑制性RNA的新型遗传构建体。 抑制性RNA可以是反义RNA或共抑制RNA，并且起减少目标细胞中目的基因表达的作用。 本发明的遗传结构能够在真核细胞的细胞质中复制并且包含与编码多核苷酸的功能性组合的启动子区。 遗传结构可以设计成在植物细胞，酵母细胞和哺乳动物细胞的细胞质中复制。 当感兴趣的真核细胞是植物细胞时，遗传构建优选衍生自植物RNA病毒。 植物RNA病毒衍生的遗传结构可以使用植物病毒亚基因组启动子，包括与RNA编码区域功能组合的来自烟草花叶病毒的亚基因组启动子。 在本发明的一个优选实施方案中，诱导植物细胞产生升高水平的类胡萝卜素八氢番茄红素。 通过使用本发明的载体抑制酶八氢番茄红素去饱和酶的表达来实现提高的八氢番茄聚糖水平。
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公开(公告)号：US20050204422A1
公开(公告)日：2005-09-15
申请号：US11129170
申请日：2005-05-13
IPC分类号： A01H5/00 , C07K14/08 , C07K14/095 , C07K14/415 , C07K14/705 , C12N1/19 , C12N5/10 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/24 , C12N9/72 , C12N9/78 , C12N9/84 , C12N15/09 , C12N15/113 , C12N15/12 , C12N15/40 , C12N15/53 , C12N15/82 , C12N15/83 , C12N15/86 , C12P21/04 , C12P41/00 , A01H1/00
CPC分类号： C12N15/1137 , C07K14/005 , C07K14/415 , C07K14/70571 , C12N9/00 , C12N9/0059 , C12N9/0071 , C12N9/0083 , C12N9/1074 , C12N9/1085 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8218 , C12N15/8249 , C12N15/825 , C12N15/8289 , C12N15/86 , C12N2710/22043 , C12N2710/22062 , C12N2750/12043 , C12N2770/00022 , C12N2770/00043 , C12N2770/00051 , C12N2770/32643 , C12N2770/32662 , C12N2770/32722 , C12N2770/32743 , C12N2770/32762 , C12N2770/36143 , C12N2770/36162 , C12N2799/021 , C12N2810/609 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , G01N2333/4353
摘要： The invention provides novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA, and functions to reduce the expression of a gene of interest in the target cell. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide. The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells. When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus. Plant RNA virus derived genetic constructions may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. In a preferred embodiment of the invention, plant cells are induced to produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression of the enzyme phytoene desaturase using the vectors of the invention.
摘要翻译： 本发明提供了用于在真核细胞的细胞质中表达抑制性RNA的新型遗传构建体。 抑制性RNA可以是反义RNA或共抑制RNA，并且起减少目标细胞中目的基因表达的作用。 本发明的遗传结构能够在真核细胞的细胞质中复制并且包含与编码多核苷酸的功能性组合的启动子区。 遗传结构可以设计成在植物细胞，酵母细胞和哺乳动物细胞的细胞质中复制。 当感兴趣的真核细胞是植物细胞时，遗传构建优选衍生自植物RNA病毒。 植物RNA病毒衍生的遗传结构可以使用植物病毒亚基因组启动子，包括与RNA编码区域功能组合的来自烟草花叶病毒的亚基因组启动子。 在本发明的一个优选实施方案中，诱导植物细胞产生升高水平的类胡萝卜素八氢番茄红素。 通过使用本发明的载体抑制酶八氢番茄红素去饱和酶的表达来实现提高的八氢番茄聚糖水平。
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