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    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    6.
    发明授权
    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07198937B2

    公开(公告)日：2007-04-03

    申请号：US11024545

    申请日：2004-12-29

    申请人： Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    发明人： Zhixiong Xue ,  Narendra S. Yadav ,  Daniel Joseph Macool

    IPC分类号： C12N1/16 ,  C12N1/13 ,  C12N1/19 ,  C12N1/18 ,  C12N9/10 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    摘要： An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译： 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
    10.
    发明授权
    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08323935B2

    公开(公告)日：2012-12-04

    申请号：US12244822

    申请日：2008-10-03

    申请人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C07D293/10

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

    摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.

    摘要翻译： 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 描述优选的基因敲除，以及在宿主细胞内生产EPA的方法以及来自优化的解脂耶氏酵母菌株的包含EPA的产物的方法。