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1.发明授权Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和含油生物体中的油含量公开(公告)号：US08685681B2
公开(公告)日：2014-04-01
申请号：US12338536
申请日：2008-12-18
申请人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
发明人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
CPC分类号： C12N9/1029 , C12P7/6463 , C12P7/6472
摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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2.发明授权Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms 失效酰基转移酶调节以增加含油生物的总脂质和油中多不饱和脂肪酸的百分比公开(公告)号：US08685679B2
公开(公告)日：2014-04-01
申请号：US11190750
申请日：2005-07-27
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the activity of an organism's DAG ATs (e.g., phospholipid:diacylglycerol acyltransferase (PDAT) and/or diacylglycerol acyltransferase 1 (DGAT1) and/or diacylglycerol acyltransferase 2 (DGAT2)) to thereby reduce the overall rate of oil biosynthesis while concomitantly increasing the percent of PUFAs that are incorporated into the lipid and oil fractions. The teachings herein will thereby enable one to engineer a wide variety of oleaginous organisms to produce oils with very specific fatty acid compositions.
摘要翻译： 本文通过调节特异性酰基转移酶的活性来提高增加多不饱和脂肪酸（PUFA）在产生PUFA的含油生物体的总脂质和油中的百分数。 具体地说，由于油生物合成预期在含油期间与多不饱和度竞争，有可能减少或失活生物体的DAG AT（例如磷脂：二酰基甘油酰基转移酶（PDAT）和/或二酰基甘油酰基转移酶1（DGAT1））和/或 二酰基甘油酰基转移酶2（DGAT2）），从而降低油的合成速率，同时增加掺入脂质和油馏分中的PUFA的百分比。 因此，本文的教导使得能够设计各种含油生物以产生具有非常特定的脂肪酸组成的油。
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3.发明授权Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产公开(公告)号：US08637298B2
公开(公告)日：2014-01-28
申请号：US12814815
申请日：2010-06-14
IPC分类号： C12N1/00
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶 合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。
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公开(公告)号：US07879591B2
公开(公告)日：2011-02-01
申请号：US12485141
申请日：2009-06-16
CPC分类号： C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
摘要： Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
摘要翻译： 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便能够在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加和降低转化效率。
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5.发明授权Δ12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts 有权＆Dgr; 12去饱和酶基因适用于改变油质酵母中多不饱和脂肪酸的含量公开(公告)号：US07749703B2
公开(公告)日：2010-07-06
申请号：US11494318
申请日：2006-07-27
申请人： Narendra S. Yadav , Hongxiang Zhang
发明人： Narendra S. Yadav , Hongxiang Zhang
CPC分类号： C12N9/0083
摘要： The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
摘要翻译： 本发明涉及能够催化油酸向亚油酸（LA; 18：2）转化的脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主微生物。 增加特定ω-3和/或ω-6脂肪酸生产的方法是通过过表达'Dgr。12脂肪酸去饱和酶或通过破坏天然基因来描述的。
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公开(公告)号：US20090325265A1
公开(公告)日：2009-12-31
申请号：US12485141
申请日：2009-06-16
CPC分类号： C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
摘要： Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
摘要翻译： 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加Delta4去饱和转化效率。
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公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
申请人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
摘要翻译： 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
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8.发明申请DELTA-15 DESATURASE GENES SUITABLE FOR INCREASING LEVELS OF OMEGA-3 FATTY ACIDS 有权适用于增加OMEGA-3脂肪酸水平的DELTA-15去饱和基因公开(公告)号：US20090274816A1
公开(公告)日：2009-11-05
申请号：US12420345
申请日：2009-04-08
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.
摘要翻译： 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。
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9.发明授权公开(公告)号：US07556949B2
公开(公告)日：2009-07-07
申请号：US11779915
申请日：2007-07-19
申请人： Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
发明人： Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
CPC分类号： C12P7/6427 , C12N9/0083 , C12P7/6472
摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.
摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。
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10.发明申请Diacylglycerol Acyltransferases for Alteration of Polyunsaturated Fatty Acids and Oil Content in Oleaginous Organisms 有权用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和油性生物中的油含量公开(公告)号：US20090104674A1
公开(公告)日：2009-04-23
申请号：US12338536
申请日：2008-12-18
申请人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
发明人： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
IPC分类号： C12P7/64
CPC分类号： C12N9/1029 , C12P7/6463 , C12P7/6472
摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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