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公开(公告)号：US08377715B2
公开(公告)日：2013-02-19
申请号：US12767659
申请日：2010-04-26
申请人： Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人： Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号： G01N1/18
CPC分类号： C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要： The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译： 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。
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公开(公告)号：US07667010B2
公开(公告)日：2010-02-23
申请号：US11508582
申请日：2006-08-22
摘要： The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. The invention further provides reagents, columns and instrumentation related to this and other methods.
摘要翻译： 本发明尤其提供从溶液中提取分析物的方法，包括以下步骤：将含有分析物的溶液通过具有固相萃取表面的提取通道，由此分析物吸附到所述提取通道的提取表面; 从所述提取通道清洗大量液体; 并通过使解吸溶剂通过通道洗脱分析物。 本发明还提供了与该方法和其它方法相关的试剂，色谱柱和仪器。
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公开(公告)号：US11274292B2
公开(公告)日：2022-03-15
申请号：US15585109
申请日：2017-05-02
申请人： PHYNEXUS, INC.
IPC分类号： C12N15/10 , G01N35/10 , B01D15/42 , B01D15/12 , C12Q1/6806 , B01D15/20 , G01N35/00 , B01D15/32 , B01D15/36
摘要： The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.
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公开(公告)号：US08501116B2
公开(公告)日：2013-08-06
申请号：US13206435
申请日：2011-08-09
IPC分类号： B01L3/00
CPC分类号： C07K16/26 , B01D15/325 , B01D15/327 , B01D15/3804 , B01J20/285 , B01J20/291 , B01J2220/64 , B01L3/0275 , B01L2200/0631 , B82Y30/00 , G01N2030/009
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.
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公开(公告)号：US08362219B2
公开(公告)日：2013-01-29
申请号：US12685485
申请日：2010-01-11
摘要： The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.
摘要翻译： 本发明尤其提供从溶液中提取分析物的方法，包括以下步骤：将含有分析物的溶液通过具有固相萃取表面的提取通道，由此分析物吸附到所述提取通道的提取表面; 从所述提取通道清洗大量液体; 并通过使解吸溶剂通过通道洗脱分析物。 在一些实施方案中，分析物是蛋白质。 本发明还提供了与该方法和其它方法相关的试剂，通道，柱和仪器。
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公开(公告)号：US07722820B2
公开(公告)日：2010-05-25
申请号：US11285531
申请日：2005-11-21
申请人： Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人： Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号： B01D15/08
CPC分类号： G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides methods characterized by the use of hydrophobic and/or hydrophilic membranes as frits, and/or by the inclusion of a wad of fibrous material to act as a wicking agent.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床，通常在两个玻璃料之间。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以疏水和/或亲水膜为玻璃料，和/或通过包含纤维材料的纤维作为芯吸剂的特征的方法。
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公开(公告)号：US08679857B2
公开(公告)日：2014-03-25
申请号：US13752227
申请日：2013-01-28
申请人： Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
发明人： Chris Suh , Lee Hoang , Jennifer E. Grant , Douglas T. Gjerde
IPC分类号： C12N15/10
CPC分类号： C12N15/101 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/58 , B01J2220/64 , B82Y30/00 , G01N1/405 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , B01D15/3804
摘要： The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
摘要翻译： 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。
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公开(公告)号：US08361806B2
公开(公告)日：2013-01-29
申请号：US12986102
申请日：2011-01-06
CPC分类号： G01N1/34 , G01N1/40 , G01N1/405 , G01N30/00 , G01N30/06 , G01N30/08 , G01N2030/009 , G01N2030/062 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： The invention provides, inter alia, methods, devices and reagents for the preparation of native and non-denatured biomolecules using solid-phase extraction channels. The invention is particularly suited for the purification, concentration and/or analysis of protein analytes. The invention further provides, inter alia, methods, devices and reagents for the purification, concentration and/or analysis of multi-protein complexes.
摘要翻译： 本发明尤其提供了使用固相萃取通道制备天然和非变性生物分子的方法，装置和试剂。 本发明特别适用于蛋白质分析物的纯化，浓缩和/或分析。 本发明尤其还提供用于多蛋白复合物的纯化，浓缩和/或分析的方法，装置和试剂。
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公开(公告)号：US08148168B2
公开(公告)日：2012-04-03
申请号：US12837141
申请日：2010-07-15
申请人： Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
发明人： Douglas T. Gjerde , Allen Burge , Ronald Jones , Mark Abel
IPC分类号： G01N1/18
CPC分类号： G01N1/40 , B01J20/285 , B01J20/286 , B01J20/291 , B01J20/3244 , B01J2220/54 , B01J2220/64 , B82Y30/00 , G01N30/02 , G01N30/603 , G01N30/6065 , G01N2035/1053 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column, often between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the extraction columns are comprised of frits having a low pore. In some embodiments, the frits of the extraction columns have a pore volume of less than one microliter or less than 10% of the interstitial volume of the bed of extraction media.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括定位在柱中的提取介质床，通常在两个玻璃料之间。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，萃取柱由具有低孔的玻璃料组成。 在一些实施方案中，提取塔的玻璃料的孔体积小于提取介质床的间隙体积的小于1微升或小于10％。
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公开(公告)号：US08057668B2
公开(公告)日：2011-11-15
申请号：US12914874
申请日：2010-10-28
IPC分类号： B01J20/291 , B01D15/08 , B01J20/00
CPC分类号： G01N35/10 , G01N2035/103 , G01N2035/1053
摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the invention provides columns characterized by low backpressure.
摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 柱通常包括位于两个玻璃料之间的柱中的提取介质床。 在一些实施方案中，提取柱使用改进的移液管尖作为柱体。 在一些实施方案中，本发明提供了以低背压为特征的色谱柱。
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