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21 条记录 (耗时 0.034 秒)

    Low Dead Volume Extraction Column Device
    2.
    发明申请
    Low Dead Volume Extraction Column Device 有权
    低死体积提取柱装置

    公开(公告)号：US20110293490A1

    公开(公告)日：2011-12-01

    申请号：US13206435

    申请日：2011-08-09

    申请人： Douglas T. Gjerde ,  Christopher P. Hanna

    发明人： Douglas T. Gjerde ,  Christopher P. Hanna

    IPC分类号： B01L3/02

    CPC分类号： C07K16/26 ,  B01D15/325 ,  B01D15/327 ,  B01D15/3804 ,  B01J20/285 ,  B01J20/291 ,  B01J2220/64 ,  B01L3/0275 ,  B01L2200/0631 ,  B82Y30/00 ,  G01N2030/009

    摘要： The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The invention is characterized by the use of low dead volume columns, which is achieved in part by the use of low pore volume frits (e.g., membrane screens) to contain a bed of extraction media in the column. Low dead volume facilitates the elution of the captured analyte into a very small volume of desorption solution, allowing for the preparation of low volume samples containing relatively high concentrations of analyte.

    摘要翻译： 本发明提供了用于从样品溶液中纯化分析物（例如生物大分子，如肽，蛋白质或核酸）的提取柱，以及制备和使用这些色谱柱的方法。 本发明的特征在于使用低死体积柱，其部分通过使用低孔体积玻璃料（例如膜筛）在柱中含有提取介质床而实现。 低死体积有助于将捕获的分析物溶解到非常小体积的解吸溶液中，允许制备含有相对高浓度分析物的低体积样品。

    Open channel solid phase extraction systems and methods
    4.
    发明授权
    Open channel solid phase extraction systems and methods 有权
    开放通道固相萃取系统及方法

    公开(公告)号：US08362219B2

    公开(公告)日：2013-01-29

    申请号：US12685485

    申请日：2010-01-11

    申请人： Douglas T. Gjerde ,  Christopher P. Hanna

    发明人： Douglas T. Gjerde ,  Christopher P. Hanna

    IPC分类号： C07K1/22 ,  B01D15/00 ,  G01N30/02

    CPC分类号： G01N1/34 ,  G01N1/40 ,  G01N1/405

    摘要： The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.

    摘要翻译： 本发明尤其提供从溶液中提取分析物的方法，包括以下步骤：将含有分析物的溶液通过具有固相萃取表面的提取通道，由此分析物吸附到所述提取通道的提取表面; 从所述提取通道清洗大量液体; 并通过使解吸溶剂通过通道洗脱分析物。 在一些实施方案中，分析物是蛋白质。 本发明还提供了与该方法和其它方法相关的试剂，通道，柱和仪器。

    Method and system for RNA analysis by matched ion polynucleotide chromatography
    5.
    发明授权
    Method and system for RNA analysis by matched ion polynucleotide chromatography 失效
    通过匹配离子多核苷酸色谱法进行RNA分析的方法和系统

    公开(公告)号：US06576133B2

    公开(公告)日：2003-06-10

    申请号：US09753856

    申请日：2001-01-02

    申请人： Douglas T. Gjerde ,  David P. Hornby ,  Christopher P. Hanna ,  Alexander I. Kuklin ,  Robert M. Haefele ,  Paul D. Taylor

    发明人： Douglas T. Gjerde ,  David P. Hornby ,  Christopher P. Hanna ,  Alexander I. Kuklin ,  Robert M. Haefele ,  Paul D. Taylor

    IPC分类号： B01D1508

    CPC分类号： B01D15/366 ,  C12N15/101

    摘要： A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a polymeric separation medium having non-polar surfaces and eluting the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The preferred surfaces are characterized by being substantially free from multivalent cations which are free to interfere with RNA segregation. The elution is preferably performed at a temperature sufficient to denature the RNA. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm. Examples of separation media include beads and monolithic columns.

    摘要翻译： 匹配的离子多核苷酸色谱法和基于尺寸的RNA分子混合物分离的系统。 该方法包括将混合物施加到具有非极性表面的聚合物分离介质中，并用包含抗衡离子试剂和有机组分的流动相洗脱RNA分子。 优选的表面的特征在于基本上不含可以干扰RNA分离的多价阳离子。 洗脱优选在足以使RNA变性的温度下进行。 该方法可用于分离长度在约100至20,000个核苷酸范围内的RNA分子。 使用ID大于约5mm的色谱柱获得改进的分离。 分离介质的实例包括珠粒和整体柱。

    Open Channel Solid Phase Extraction Systems and Methods
    7.
    发明申请
    Open Channel Solid Phase Extraction Systems and Methods 有权
    开放通道固相萃取系统和方法

    公开(公告)号：US20100111771A1

    公开(公告)日：2010-05-06

    申请号：US12685485

    申请日：2010-01-11

    申请人： Douglas T. Gjerde ,  Christopher P. Hanna

    发明人： Douglas T. Gjerde ,  Christopher P. Hanna

    IPC分类号： G01N33/53

    CPC分类号： G01N1/34 ,  G01N1/40 ,  G01N1/405

    摘要： The invention provides, inter alia, methods of extracting an analyte from a solution comprising the steps of: passing a solution containing an analyte through an extraction channel having a solid phase extraction surface, whereby analyte adsorbs to the extraction surface of said extraction channel; purging bulk liquid from said extraction channel; and eluting the analyte by passing a desorption solvent through the channel. In some embodiments, the analyte is a protein. The invention further provides reagents, channels, columns and instrumentation related to this and other methods.

    摘要翻译： 本发明尤其提供从溶液中提取分析物的方法，包括以下步骤：将含有分析物的溶液通过具有固相萃取表面的提取通道，由此分析物吸附到所述提取通道的提取表面; 从所述提取通道清洗大量液体; 并通过使解吸溶剂通过通道洗脱分析物。 在一些实施方案中，分析物是蛋白质。 本发明还提供了与该方法和其它方法相关的试剂，通道，柱和仪器。

    Apparatus and method for separating and purifying polynucleotides
    10.
    发明授权
    Apparatus and method for separating and purifying polynucleotides 有权
    用于分离和纯化多核苷酸的装置和方法

    公开(公告)号：US06265168B1

    公开(公告)日：2001-07-24

    申请号：US09318407

    申请日：1999-05-25

    申请人： Douglas T. Gjerde ,  Christopher P. Hanna ,  Paul D. Taylor ,  Benjamin L. Legendre, Jr. ,  Robert M. Haefele

    发明人： Douglas T. Gjerde ,  Christopher P. Hanna ,  Paul D. Taylor ,  Benjamin L. Legendre, Jr. ,  Robert M. Haefele

    IPC分类号： C12Q168

    CPC分类号： B01D15/366 ,  B01J20/287

    摘要： A method for removing a target DNA fragment having a predetermined base-pair length from a mixture of DNA fragments comprises the following steps. A mixture of DNA fragments which may contain the target DNA fragments is applied to a separation column containing media having a nonpolar, nonporous surface, the mixture of DNA fragments being in a first solvent mixture containing a counterion and a DNA binding concentration of driving solvent in a cosolvent. The target DNA fragments are separated from the media by contacting it with a second solvent solution containing a counterion and a concentration of driving solvent in cosolvent which has been predetermined to remove DNA fragments having the target DNA fragment base pair length from the media. The target DNA fragments can be collected and optionally amplified. When the method is being applied to collect a putative fragment, if present, no DNA fragments having the base pair length of the target DNA could be present in the mixture. Alternatively, DNA fragments having the base pair length of the target DNA are present in the mixture. The disclosure also describes an ambient or low pressure device for separating polynucleotide fragments from a mixture of polynucleotide fragments comprises a tube having an upper solution input chamber, a lower eluant receiving chamber, and a fixed unit of separation media supported therein. The separation media has nonpolar separation surfaces which are free from multivalent cations which would react with counterion to form an insoluble polar coating on the surface of the separation media.

    摘要翻译： 从DNA片段的混合物中除去具有预定碱基对长度的靶DNA片段的方法包括以下步骤。 将含有靶DNA片段的DNA片段的混合物应用于含有非极性，无孔表面的培养基的分离塔，DNA片段的混合物在含有抗衡离子的第一溶剂混合物和驱动溶剂的DNA结合浓度 共溶剂 通过使目标DNA片段与含有抗衡离子的溶剂溶液和在助溶剂中的驱动溶剂浓度的第二溶剂溶液接触来分离，所述助溶剂已被预先从介质中除去具有靶DNA片段碱基对长度的DNA片段。 可以收集目标DNA片段并任选地扩增。 当该方法被应用于收集假定片段时（如果存在），则在混合物中不能存在具有目标DNA碱基对长度的DNA片段。 或者，具有靶DNA的碱基对长度的DNA片段存在于混合物中。 本公开还描述了用于从多核苷酸片段的混合物分离多核苷酸片段的环境或低压装置，包括具有上溶液输入室，下洗脱液接收室和支撑在其中的分离介质的固定单元的管。 分离介质具有非极性分离表面，其不含多价阳离子，其将与抗衡离子反应以在分离介质的表面上形成不溶性极性涂层。