公开(公告)号：US07074900B2

公开(公告)日：2006-07-11

申请号：US10422422

申请日：2003-04-23

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

IPC分类号： C07K14/00 ,  C07K14/415 ,  A61K39/35 ,  A61K39/36

CPC分类号： A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y10S436/904 ,  Y10T436/182

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

公开(公告)号：US06555116B1

公开(公告)日：2003-04-29

申请号：US09238379

申请日：1999-01-27

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihon C. Yee ,  Oscar L. Frick

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihon C. Yee ,  Oscar L. Frick

IPC分类号： A61K3936

CPC分类号： A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y10S436/904 ,  Y10T436/182

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

摘要翻译： 硫氧还蛋白，一种小二硫醇蛋白，是一种特异性的还原剂，适用于过敏原蛋白，特别是花粉和动植物来源的变应原蛋白。 所有靶向蛋白含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。 蛋白质是过敏性活性的，在氧化（S-S）状态下消化不良。 当减少（SH状态）时，它们失去过敏原性和/或变得更易消化。 当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或硫辛酸化学还原剂被活化（还原）时，硫氧还蛋白实现了这一还原。 用致敏狗进行的皮肤试验表明，在注射前用降低的硫氧还蛋白处理花粉消除或降低了花粉的致敏性。 研究显示硫氧还蛋白还原后，通过胃蛋白酶消化花粉蛋白。 通过硫氧还蛋白还原的花粉蛋白是有效和安全的免疫治疗剂，用于减少或消除否则在暴露于未还原花粉蛋白时会发生的动物过敏反应。

公开(公告)号：US06838113B1

公开(公告)日：2005-01-04

申请号：US09296927

申请日：1999-04-22

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

IPC分类号： A21D2/26 ,  A21D2/36 ,  A21D8/04 ,  A21D13/06 ,  A23C9/12 ,  A23C9/152 ,  A23C9/20 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/16 ,  A23J3/18 ,  A23K1/16 ,  A23L1/015 ,  A23L1/16 ,  A23L1/182 ,  A23L1/211 ,  A23L1/305 ,  A23L1/314 ,  A61K38/44 ,  A61K39/35 ,  C07K1/113 ,  C07K14/415 ,  C07K14/435 ,  C07K14/46 ,  C07K14/76 ,  C07K14/81 ,  C12N9/02 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  A23L1/29 ,  A23L3/358

CPC分类号： A21D13/064 ,  A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K38/44 ,  A61K39/35 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  A61K2300/00

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

公开(公告)号：US5952034A

公开(公告)日：1999-09-14

申请号：US953703

申请日：1997-10-17

申请人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

发明人： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Jin-an Jiao ,  Joshua H. Wong ,  Boihon C. Yee

IPC分类号： A21D2/26 ,  A21D2/36 ,  A21D8/04 ,  A21D13/06 ,  A23C9/12 ,  A23C9/152 ,  A23C9/20 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/16 ,  A23J3/18 ,  A23K1/16 ,  A23L1/015 ,  A23L1/16 ,  A23L1/182 ,  A23L1/211 ,  A23L1/305 ,  A23L1/314 ,  A61K38/44 ,  A61K39/35 ,  C07K1/113 ,  C07K14/415 ,  C07K14/435 ,  C07K14/46 ,  C07K14/76 ,  C07K14/81 ,  C12N9/02 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  A23L1/03

CPC分类号： A21D13/064 ,  A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A23L5/27 ,  A23L7/109 ,  A23L7/196 ,  A61K38/44 ,  A61K39/35 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009

摘要： Thioredoxin, a small dithiol protein, is a specific reductant for major food proteins, allergenic proteins and particularly allergenic proteins present in widely used foods from animal and plant sources. All targeted proteins contain disulfide (S--S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S--S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food. Studies showed increased digestion of food and food proteins by pepsin and trypsin following reduction by thioredoxin.

摘要翻译： 硫氧还蛋白，一种小二硫醇蛋白，是主要的食品蛋白质，过敏原蛋白质，特别是来自动物和植物来源广泛使用的食品中的过敏原蛋白质的特异性还原剂。 所有靶向蛋白含有通过硫氧还蛋白还原成巯基（SH）水平的二硫键（S-S）键。 蛋白质是过敏性活性的，在氧化（S-S）状态下消化不良。 当减少（SH状态）时，它们失去过敏原性和/或变得更易消化。 当NADPH通过NADP-硫氧还蛋白还原酶（生理条件）或二硫苏糖醇（化学还原剂）活化（还原）时，硫氧还蛋白实现了这种还原。 用敏化狗进行的皮肤试验和喂养实验表明，在摄取之前用硫氧还蛋白降低的食物处理消除或降低了食物的过敏原性。 研究显示硫氧还蛋白还原后胃蛋白酶和胰蛋白酶消化食物和食物蛋白质。