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    Inverse labeling method for the rapid identification of marker/target proteins
    4.
    发明申请
    Inverse labeling method for the rapid identification of marker/target proteins 审中-公开
    用于快速鉴定标记/靶蛋白的逆标记方法

    公开(公告)号：US20040106150A1

    公开(公告)日：2004-06-03

    申请号：US10412964

    申请日：2003-04-14

    发明人： Yingqi Karen Wang

    IPC分类号： G01N033/53

    CPC分类号： G01N33/60 ,  G01N33/58 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2223/6265 ,  G01N2458/15

    摘要： A novel procedure for performing protein labeling for comparative proteomics termed inverse labeling is provided for the rapid identification of marker or target proteins. With this method, to evaluate protein expression of a disease or a drug treated sample in comparison with a control sample, two converse collaborative labeling experiments are performed in parallel. In one experiment the perturbed sample (by disease or by drug treatment) is isotopically heavy-labeled, whereas, the control is isotopically heavy-labeled in the second experiment. When mixed and analyzed with its unlabeled or isotope light counterpart for differential comparison, a characteristic inverse labeling pattern is observed between the two parallel analyses for proteins that are differentially-expressed to an appreciable level. In particularly useful embodiments, protein labeling is achieved through proteolytic 18O-incorporation into peptides as a result of proteolysis performed in 18O-water, metabolic incorporation of 15N (or 13C and 2H) into proteins, and chemically tagging proteins with an isotope-coded tag reagent such as an isotope-coded affinity tag reagent. Also provided is a novel procedure for preparing and purifying peptides from a protein solution and a novel procedure for identifying marker or target proteins, particular phosphorylated proteins, which combines the procedure for preparing and purifying peptides from a protein solution with inverse labeling.

    摘要翻译： 提供了用于比较蛋白质组学的蛋白质标记进行称为反向标记的新方法，用于快速鉴定标记或靶蛋白。 利用该方法，与对照样品相比较，为了评估疾病或药物处理样品的蛋白质表达，并行进行两次相反的协同标记实验。 在一个实验中，扰动样品（通过疾病或通过药物治疗）是同位素重标记的，而在第二个实验中，对照是同位素重标记的。 当与其未标记或同位素光对应物进行混合和分析以进行差异比较时，在差异表达到可感知水平的蛋白质的两个平行分析之间观察到特征反向标记模式。 在特别有用的实施方案中，蛋白质标记通过蛋白水解引入到肽中，作为在水中进行的蛋白水解，15 N（或13 C和< H）转化成蛋白质，并用同位素编码的标签试剂如同位素编码的亲和标签试剂化学标记蛋白质。 还提供了用于从蛋白质溶液制备和纯化肽的新方法和用于鉴定标记或靶蛋白的新方法，特定的磷酸化蛋白，其将从蛋白质溶液制备和纯化肽的方法与反向标记相结合。

    Method for locating metallic nitride inclusions in metallic alloy ingots
    5.
    发明授权
    Method for locating metallic nitride inclusions in metallic alloy ingots 失效
    金属合金锭中金属氮化物夹杂物的定位方法

    公开(公告)号：US5110546A

    公开(公告)日：1992-05-05

    申请号：US696805

    申请日：1991-05-07

    申请人： Jack C. White ,  Davis E. Traut ,  Laurance L. Oden ,  Roman A. Schmitt

    发明人： Jack C. White ,  Davis E. Traut ,  Laurance L. Oden ,  Roman A. Schmitt

    IPC分类号： G01N23/222 ,  G01N33/20

    CPC分类号： G01N33/206 ,  G01N23/222 ,  G01N2223/0745 ,  G01N2223/6265 ,  G01N2223/63

    摘要： A method of determining the location and history of metallic nitride and/or oxynitride inclusions in metallic melts. The method includes the steps of labeling metallic nitride and/or oxynitride inclusions by making a coreduced metallic-hafnium sponge from a mixture of hafnium chloride and the chloride of a metal, reducing the mixed chlorides with magnesium, nitriding the hafnium-labeled metallic-hafnium sponge, and seeding the sponge to be melted with hafnium-labeled nitride inclusions. The ingots are neutron activated and the hafnium is located by radiometric means. Hafnium possesses exactly the proper metallurgical and radiochemical properties for this use.

    摘要翻译： 确定金属熔体中金属氮化物和/或氧氮化物夹杂物的位置和历史的方法。 该方法包括以下步骤：通过从氯化铪和金属氯化物的混合物中制备一种压制的金属铪海绵来标记金属氮化物和/或氧氮化物夹杂物，用镁还原混合氯化物，氮化铪标记的金属铪 海绵，并将海绵接种以用铪标记的氮化物夹杂物熔化。 锭是中子激活的，铪是通过辐射方式定位的。 铪具有这种用途的正确的冶金和放射化学性质。