公开(公告)号：US07247495B2

公开(公告)日：2007-07-24

申请号：US10219367

申请日：2002-08-15

申请人： Aviv Amirav

发明人： Aviv Amirav

IPC分类号： G01N30/72 ,  G01N1/22 ,  H01J49/02 ,  H01J49/10 ,  H01J49/26 ,  B01D59/44

CPC分类号： H01J49/049 ,  G01N30/72 ,  G01N30/7246 ,  G01N30/7273 ,  H01J49/0068 ,  H01J49/045 ,  H01J49/147 ,  H01J49/16 ,  Y10T436/24 ,  Y10T436/25875

摘要： The invention provides a mass spectrometric method for analyzing a sample in a solution, including the steps of directing a flow of a solution containing sample compounds to be analyzed towards a supersonic nozzle having an input end and an output end; vaporizing the solution and sample prior to its expansion from the output end of said supersonic nozzle; allowing expansion of the vaporized sample and solution from said supersonic nozzle into a vacuum system, forming a supersonic molecular beam with vibrationally cold sample molecules; ionizing the vaporized sample compounds with electrons while contained as vibrationally cold molecules in said supersonic molecular beam; mass analyzing the ions formed from said sample compounds; detecting said ions formed from said sample compounds after mass analysis, and processing the data obtained from the resulting mass spectral information, for identifying and/or quantifying the chemical content of said sample. The invention also provides apparatus for analyzing a sample in a solution.

摘要翻译： 本发明提供了一种用于分析溶液中的样品的质谱方法，包括以下步骤：将含有待分析样品化合物的溶液流引向具有输入端和输出端的超音速喷嘴; 在从所述超音速喷嘴的输出端膨胀之前蒸发溶液和样品; 允许将蒸发的样品和溶液从所述超音速喷嘴扩展到真空系统中，形成具有振动冷样品分子的超音速分子束; 用电子电离蒸发的样品化合物，同时在所述超音速分子束中作为振动冷分子包含; 质量分析由所述样品化合物形成的离子; 在质量分析之后检测由所述样品化合物形成的离子，并处理由所得质谱信息获得的数据，以识别和/或定量所述样品的化学成分。 本发明还提供了用于分析解决方案中的样本的装置。

公开(公告)号：US06965106B2

公开(公告)日：2005-11-15

申请号：US10487506

申请日：2002-08-23

申请人： Li Ding ,  Michael Sudakov

发明人： Li Ding ,  Michael Sudakov

IPC分类号： G01N27/62 ,  H01J49/42

CPC分类号： H01J49/424 ,  H01J49/0068

摘要： A method of trapping ions using a quadrupole ion trap device includes applying quadrupole excitation to trapped precursor ions causing them to be driven into the ring electrode where they undergo surface induced dissociation. The resultant product ions are then trapped within the ion trap device.

摘要翻译： 使用四极离子阱捕获装置捕获离子的方法包括对捕获的前体离子施加四极杆激发，使得它们被驱动到环状电极中，在那里它们经历表面诱导的解离。 然后将所得产物离子捕获在离子阱装置内。

公开(公告)号：US06744040B2

公开(公告)日：2004-06-01

申请号：US09880717

申请日：2001-06-13

申请人： Melvin A. Park

发明人： Melvin A. Park

IPC分类号： H01J4906

CPC分类号： H01J49/063 ,  H01J49/005 ,  H01J49/0068

摘要： A means and method are disclosed whereby ions from an ion source can be selected and transferred to a time-of-flight mass analyzer via an arrangement of multipoles in such a way that fragmented ions may be generated by collision-induced dissociation or surface-induced dissociation. First, ions from the source are collisionally cooled by a first multipole. Second, the m/z range of the ions is then selected by a second multipole (preferably a quadrupole). Third, the selected ions are allowed to collide with a “collision surface” capable of producing fragment ions. Fourth, these fragment ions are collisionally cooled in a third multipole and delivered into a TOF mass analyzer for subsequent analysis of the fragmented ions.

摘要翻译： 公开了一种方法和方法，其中来自离子源的离子可以通过多极的排列被选择并转移到飞行时间质量分析器，使得碎片离子可以通过碰撞诱导的解离或表面诱导产生 解离 首先，来自源的离子被第一多极碰撞冷却。 其次，然后通过第二多极（优选四极）选择离子的m / z范围。 第三，允许所选择的离子与能够产生碎片离子的“碰撞表面”碰撞。 第四，这些碎片离子在第三个多极中被碰撞冷却，并输送到TOF质量分析器中，用于随后分析碎片离子。

公开(公告)号：US20030042412A1

公开(公告)日：2003-03-06

申请号：US09880717

申请日：2001-06-13

发明人： Melvin A. Park

IPC分类号： H01J049/26

CPC分类号： H01J49/063 ,  H01J49/005 ,  H01J49/0068

摘要： A means and method are disclosed whereby ions from an ion source can be selected and transferred to a time-of-flight mass analyzer via an arrangement of multipoles in such a way that fragmented ions may be generated by collision-induced dissociation or surface-induced dissociation. First, ions from the source are collisionally cooled by a first multipole. Second, the m/z range of the ions is then selected by a second multipole (preferably a quadrupole). Third, the selected ions are allowed to collide with a nullcollision surfacenull capable of producing fragment ions. Fourth, these fragment ions are collisionally cooled in a third multipole and delivered into a TOF mass analyzer for subsequent analysis of the fragmented ions.

摘要翻译： 公开了一种方法和方法，其中来自离子源的离子可以通过多极的排列被选择并转移到飞行时间质量分析器，使得碎片离子可以通过碰撞诱导的解离或表面诱导产生 解离 首先，来自源的离子被第一多极碰撞冷却。 其次，然后通过第二多极（优选四极）选择离子的m / z范围。 第三，允许所选择的离子与能够产生碎片离子的“碰撞表面”碰撞。 第四，这些碎片离子在第三个多极中被碰撞冷却，并输送到TOF质量分析器中，用于随后分析碎片离子。

公开(公告)号：US11668719B2

公开(公告)日：2023-06-06

申请号：US16647955

申请日：2018-09-20

申请人： THE TRUSTEES OF INDIANA UNIVERSITY

发明人： Martin F. Jarrold ,  Corrine A. Lutomski

IPC分类号： G01N33/68 ,  G01N33/92 ,  H01J49/40 ,  H01J49/42 ,  H01J49/00 ,  H01J49/02

CPC分类号： G01N33/6848 ,  G01N33/92 ,  H01J49/0068 ,  H01J49/025 ,  H01J49/40 ,  H01J49/4225

摘要： The present disclosure relates to a method of identifying components present in a lipoprotein. Methods provided include single particle mass spectrometry, such as charge detection mass spectrometry (CDMS). Distinct subpopulations that exist within lipoprotein classes are determined by correlating m/z and mass.

公开(公告)号：US07816644B2

公开(公告)日：2010-10-19

申请号：US11506933

申请日：2006-08-18

申请人： Steven M. Fischer ,  Harry F. Prest ,  Robert Lee, Jr.

发明人： Steven M. Fischer ,  Harry F. Prest ,  Robert Lee, Jr.

IPC分类号： H01J49/26

CPC分类号： H01J49/0059 ,  G01N33/6848 ,  G01N33/6851 ,  H01J49/0068

摘要： The invention provides a system apparatus and methods for fragmenting various molecules. In particular, the invention may be employed for fragmenting biomolecules like peptides to determine sequence information. The invention provides a mass spectrometry system for photo-activated collision induced dissociation. The mass spectrometry system or device includes an ion source for producing ions, a photon source adjacent to the ion source for photo-activating ions produced by the ion source, an electrical element adjacent to the photon source for creating an electric field for accelerating ions produced by the ion source and photo-activated by the photon source; wherein ions are produced by the ion source, photo-activated by the photon source and accelerated into a surface to cause dissociation of the activated ions; and a detector downstream from the ion source for detecting the collision induced and dissociated ions.

摘要翻译： 本发明提供了一种分散各种分子的系统装置和方法。 特别地，本发明可用于片段生物分子如肽以确定序列信息。 本发明提供了一种用于光激活碰撞诱导解离的质谱系统。 质谱系统或装置包括用于产生离子的离子源，邻近于离子源的光子源，用于由离子源产生的光激活离子;邻近光子源的电元件，用于产生用于加速产生的离子的电场 由离子源和光子源激活; 其中离子由离子源产生，由光子源光激活并加速到表面以引起活化离子的解离; 以及用于检测碰撞诱导和离解离子的离子源下游的检测器。

公开(公告)号：US20040232328A1

公开(公告)日：2004-11-25

申请号：US10487506

申请日：2004-04-01

发明人： Li Ding ,  Michael Sudakov

IPC分类号： H01J049/00

CPC分类号： H01J49/424 ,  H01J49/0068

摘要： A method of trapping a ions using a quadrupole ion trap device includes applying quadrupole excitation to trapped precursor ions causing them to be driven into the ring electrode where they undergo surface induced dissociation. The resultant product ions are then trapped within the ion trap device.

摘要翻译： 使用四极离子捕获装置捕获离子的方法包括将四极杆激发施加到捕获的前体离子，使其被驱动进入环状电极，在那里它们经历表面诱导的解离。 然后将所得产物离子捕获在离子阱装置内。

公开(公告)号：US06683301B2

公开(公告)日：2004-01-27

申请号：US10056671

申请日：2002-01-25

申请人： Craig Whitehouse ,  David G. Welkie

发明人： Craig Whitehouse ,  David G. Welkie

IPC分类号： H01J4916

CPC分类号： H01J49/40 ,  B82Y30/00 ,  H01J49/0068

摘要： A Time-Of-Flight mass spectrometer is configured with a pulsing region and electronic controls that generate a potential well for ions in the pulsing region, due to the repelling effect of a high-frequency electric field that is created in the space immediately proximate to a surface, and an additional static electric field that accelerates ions toward the surface. Ions can be constrained and accumulated over time in the potential well prior to acceleration into the Time-Of-Flight tube for mass analysis. Ions can also be directed to collide with the surface with high energy to cause Surface Induced Dissociation (SID) fragmentation, or with low energy to effect collisional cooling, hence, better spatial focusing, prior to mass analysis. The apparatus and methods described in the invention result in refined control of ion fragmentation energy and improved Time-Of-Flight mass analysis performance.

摘要翻译： 飞行时间质谱仪配置有脉冲区域和电子控制器，由于在紧邻的空间中产生的高频电场的排斥效应，脉冲区域中的离子产生潜在的阱 一个表面和一个额外的静电场，可以将离子加速到表面。 在加速进入飞行时间管以进行质量分析之前，离子可以在潜在的时间内受到约束和积累。 离子也可以被引导与高能量的表面碰撞以引起表面诱导解离（SID）碎裂，或者以低能量来实现碰撞冷却，因此在质量分析之前更好地进行空间聚焦。 本发明描述的装置和方法可以精确控制离子碎片能量和改进的飞行时间质量分析性能。

公开(公告)号：US06600155B1

公开(公告)日：2003-07-29

申请号：US09807796

申请日：2001-09-06

申请人： Bruce A. Andrien, Jr. ,  Michael A. Sansone ,  Allan G. Burt ,  Craig M. Whitehouse

发明人： Bruce A. Andrien, Jr. ,  Michael A. Sansone ,  Allan G. Burt ,  Craig M. Whitehouse

IPC分类号： H01J4916

CPC分类号： H01J49/40 ,  B82Y15/00 ,  B82Y30/00 ,  H01J49/0068

摘要： A Time-Of-Flight mass spectrometer (1) is configured with a pulsing region (10) and electronic controls to cause the directing of ions to a surface (12) in the Time-Of-Flight pulsing region (10). The population of ions resulting from the collecting of said ions on or near said surface (12) is subsequently accelerated into the Time-Of-Flight tube (17) for mass to charge analysis. Ions produced away from said surface (12) can be directed to the surface (12) with high or low surface collisional energies. Higher energy ion collisions with the surface (12) can result in Surface Induced Dissociation fragmentation and the resulting ion fragment population can be mass analyzed. Mass analysis can be performed prior to directing the ions to the surface allowing MS/MS Time-Of-Flight mass analysis with SID. Ion to surface low energy collisions or soft landings resulting in little or no ion fragmentation provide a means for spatially focusing ions on or near the surface prior to accelerating the surface collected ions into the Time-Of-Flight tube.

摘要翻译： 飞行时间质谱仪（1）配置有脉冲区域（10）和电子控制器以引导离子到飞行时间脉动区域（10）中的表面（12）。 随后将所述离子收集在所述表面（12）上或其附近产生的离子群随后被加速到飞行时间管（17）中以进行大量电荷分析。 离开所述表面（12）产生的离子可以以高或低的表面碰撞能量被引导到表面（12）。 与表面（12）的高能离子碰撞可导致表面诱导解离破碎，并且可以质量分析得到的离子片段。 在将离子引导到表面之前可以进行质量分析，允许使用SID进行MS / MS飞行时间质量分析。 离子到表面的低能量碰撞或软着陆导致很少或没有离子碎裂提供了在将表面收集的离子加速到飞行时间管之前将离子空间聚焦在表面上或附近的手段。

公开(公告)号：US06204500B1

公开(公告)日：2001-03-20

申请号：US09413443

申请日：1999-10-06

申请人： Craig M. Whitehouse ,  Bruce Andrien

发明人： Craig M. Whitehouse ,  Bruce Andrien

IPC分类号： H01J4916

CPC分类号： H01J49/40 ,  B82Y15/00 ,  B82Y30/00 ,  H01J49/0068

摘要： A Time-Of-Flight mass spectrometer is configured with a pulsing region and electronic controls to cause the directing of ions to a surface in the Time-Of-Flight pulsing region. The population of ions resulting from the collecting of said ions on or near said surface is subsequently accelerated into the Time-Of-Flight tube for mass to charge analysis. Ions produced away from said surface located in the pulsing region of a Time-Of-Flight mass spectrometer can be directed to the surface with high or low surface collisional energies. Higher energy ion collisions with the surface can result in Surface Induced Dissociation fragmentation and the resulting ion fragment population can be accelerated into Time-Of-Flight tube where the ions are mass to charge analyzed. Ion mass to charge selection can occur prior to directing ions to the pulsing region surface allowing MS/MS Time-Of-Flight mass analysis with SID. Ion to surface low energy collisions or soft landings resulting in little or no ion fragmentation provide a means for spatially focusing ions on or near the surface prior to accelerating the surface collected ions into the Time-Of-Flight tube. The apparatus and methods described in the invention result in refined control of ion fragmentation energy and improved Time-Of-Flight mass to charge analysis performance.

摘要翻译： 飞行时间质谱仪配置有脉冲区域和电子控制器，以将离子引导到飞行时间脉动区域中的表面。 随后在所述表面上或附近收集所述离子产生的离子群随后被加速到飞行时间管中用于质量进行电荷分析。 离开位于飞行时间质谱仪的脉冲区域的所述表面的离子可以被引导到具有高或低表面碰撞能量的表面。 与表面的高能离子碰撞可导致表面诱导解离破碎，并且所得到的离子片段群体可以加速到飞行时间管中，其中离子被质量分析。 在将离子引导到脉冲区域表面之前可以进行离子质量以进行电荷选择，从而允许使用SID的MS / MS飞行时间质量分析。 离子到表面的低能量碰撞或软着陆导致很少或没有离子碎裂提供了在将表面收集的离子加速到飞行时间管之前将离子空间聚焦在表面上或附近的手段。 本发明中描述的装置和方法导致离子断裂能量的精细控制和改进的飞行时间质量以进行电荷分析性能。