公开(公告)号：US20110160445A1

公开(公告)日：2011-06-30

申请号：US13037219

申请日：2011-02-28

Applicant: David R. Brigstock ,  Paul A. Harding

Inventor： David R. Brigstock ,  Paul A. Harding

IPC: C07H21/04

CPC classification number: C07K14/475 ,  A61K38/00 ,  C07K16/22 ,  C07K16/24 ,  C07K2317/34 ,  Y10S530/85 ,  Y10S930/12

Abstract: Substantially pure heparin-binding growth factor polypeptides (HBGFs), nucleic acids encoding the HBGFs and antibodies which bind to the HBGFs of the invention are provided. The HBGF polypeptides axe useful in methods for the induction of bone, cartilage and tissue formation, growth and development of the endometrium and in the acceleration of wound healing.

Abstract translation: 提供了基本上纯的肝素结合生长因子多肽（HBGF），编码HBGF的核酸和与本发明的HBGFs结合的抗体。 HBGF多肽可用于诱导骨，软骨和组织形成，子宫内膜的生长和发育以及加速伤口愈合的方法。

公开(公告)号：US20090233846A1

公开(公告)日：2009-09-17

申请号：US12467081

申请日：2009-05-15

Applicant: MARIUSZ W. SZKUDLINSKI ,  BRUCE D. WEINTRAUB ,  MATHIS GROSSMANN

Inventor： MARIUSZ W. SZKUDLINSKI ,  BRUCE D. WEINTRAUB ,  MATHIS GROSSMANN

IPC: A61K38/09 ,  C07K7/23 ,  C07H21/04 ,  C12N15/63 ,  C12N5/00 ,  C07K14/575

CPC classification number: C07K14/59 ,  A61K38/00 ,  Y10S530/85

Abstract: The invention is directed toward a human glycoprotein hormone having at least one, two, three, four, or five basic amino acids in the α-subunit at positions selected from the group consisting of positions 11, 13, 14, 16, 17, and 20. The invention is also directed to a human glycoprotein where at least one of the amino acids at position 58, 63, and 69 of the β-subunit of the human thyroid stimulating hormone are basic amino acids. The invention is further directed to a modified human glycoprotein hormone having increased activity over a wild-type human glycoprotein hormone, where the modified human glycoprotein comprises a basic amino acid substituted at a position corresponding to the same amino acid position in a non-human glycoprotein hormone having an increased activity over the wild-type human glycoprotein hormone. The invention is also directed to a method of constructing superactive nonchimeric analogs of human hormones comprising comparing the amino acid sequence of a more active homolog from another species to the human hormone, and selecting superactive analogs from the substituted human hormones. The invention is also directed to nucleic acids encoding the modified human glycoprotein hormones, vectors containing those nucleic acids, and host cells containing those vectors.

Abstract translation: 本发明涉及在α亚基中具有至少一个，二个，三个，四个或五个碱性氨基酸的人类糖蛋白激素，所述碱基氨基酸位于选自下组的位置：第11,13,14,16,17和 本发明还涉及人类糖蛋白，其中人类甲状腺刺激激素的β-亚基的58,63和69位的至少一个氨基酸是碱性氨基酸。 本发明进一步涉及一种与野生型人类糖蛋白激素相比具有增加的活性的修饰的人类糖蛋白激素，其中修饰的人类糖蛋白包含在非人类糖蛋白中与相同氨基酸位置相应位置取代的碱性氨基酸 具有比野生型人类糖蛋白激素增加的活性的激素。 本发明还涉及一种构建人类激素的超活性非嵌段类似物的方法，其包括将来自另一物种的更有活性同源物的氨基酸序列与人激素进行比较，以及从所述取代的人类激素中选择超活性类似物。 本发明还涉及编码修饰的人类糖蛋白激素的核酸，含有这些核酸的载体和含有这些载体的宿主细胞。

公开(公告)号：US20070275427A1

公开(公告)日：2007-11-29

申请号：US10591834

申请日：2005-03-04

Applicant: Masanobu Akimoto ,  Shigeki Katou ,  Makoto Namioka

Inventor： Masanobu Akimoto ,  Shigeki Katou ,  Makoto Namioka

IPC: G01N33/53

CPC classification number: C07K16/16 ,  C07K16/18 ,  G01N33/5308 ,  G01N33/68 ,  G01N2333/4731 ,  Y10S530/85 ,  Y10S530/868

Abstract: The present invention provides an immunological detection method that can detect milk allergens, allergens of albumen, flour, buckwheat and peanut with high sensitivity in foods containing these allergens regardless they are denatured/native, and a detection kit to be used therefor. It is a method for detecting allergens by using 2 or more monoclonal antibodies recognizing native and denatured milk allergens, native and denatured albumen allergens, native and denatured flour allergens, native and denatured buckwheat allergens, and native and denatured peanut allergens, using asl casein which is the main protein of milk casein, β-lactoglobulin which is the main protein of whey, ovalubumin and ovomucoid which are main proteins of albumen, gliadin which is the main protein of flour, protein with a molecular weight of 24 kDa and 76 kDa which are main proteins of buckwheat, and Ara h1 which is the main protein of peanut as an index.

Abstract translation: 本发明提供了一种免疫检测方法，其可以检测牛奶过敏原，含有这些变应原的食品中的蛋白质，面粉，荞麦和花生的过敏原，而不管它们是变性/天然的，以及用于其的检测试剂盒。 它是一种通过使用2种或更多种识别天然和变性乳过敏原，天然和变性白蛋白过敏原，天然和变性面粉变应原，天然和变性荞麦变应原以及天然和变性花生过敏原的单克隆抗体来检测变应原的方法，使用酪蛋白 是乳酪蛋白的主要蛋白质，β-乳球蛋白，其是乳清，卵白蛋白和卵巢粘蛋白的主要蛋白质，其是蛋白质的主要蛋白质，麦谷蛋白是麦粉的主要蛋白质，分子量为24kDa和76kDa的蛋白质 是荞麦的主要蛋白质，以花生为主要蛋白质的Ara h1为指标。

公开(公告)号：US20060183672A1

公开(公告)日：2006-08-17

申请号：US11409428

申请日：2006-04-21

Applicant: Mariusz Szkudlinski ,  Bruce Weintraub ,  Mathis Grossmann

Inventor： Mariusz Szkudlinski ,  Bruce Weintraub ,  Mathis Grossmann

IPC: C07K14/575 ,  C07H21/04 ,  A61K38/22

CPC classification number: C07K14/59 ,  A61K38/00 ,  Y10S530/85

Abstract: The invention is directed toward a human glycoprotein hormone having at least one, two, three, four, or five basic amino acids in the α-subunit at positions selected from the group consisting of positions 11, 13, 14, 16, 17, and 20. The invention is also directed to a human glycoprotein where at least one of the amino acids at position 58, 63, and 69 of the β-subunit of the human thyroid stimulating hormone are basic amino acids. The invention is further directed to a modified human glycoprotein hormone having increased activity over a wild-type human glycoprotein hormone, where the modified human glycoprotein comprises a basic amino acid substituted at a position corresponding to the same amino acid position in a non-human glycoprotein hormone having an increased activity over the wild-type human glycoprotein hormone. The invention is also directed to a method of constructing superactive nonchimeric analogs of human hormones comprising comparing the amino acid sequence of a more active homolog from another species to the human hormone, and selecting superactive analogs from the substituted human hormones. The invention is also directed to nucleic acids encoding the modified human glycoprotein hormones, vectors containing those nucleic acids, and host cells containing those vectors.

Abstract translation: 本发明涉及在α亚基中具有至少一个，二个，三个，四个或五个碱性氨基酸的人类糖蛋白激素，所述碱基氨基酸位于选自下组的位置：第11,13,14,16,17和 本发明还涉及人类糖蛋白，其中人类甲状腺刺激激素的β-亚基的58,63和69位的至少一个氨基酸是碱性氨基酸。 本发明进一步涉及一种与野生型人类糖蛋白激素相比具有增加的活性的修饰的人类糖蛋白激素，其中修饰的人类糖蛋白包含在非人类糖蛋白中与相同氨基酸位置相应位置取代的碱性氨基酸 具有比野生型人类糖蛋白激素增加的活性的激素。 本发明还涉及一种构建人类激素的超活性非嵌段类似物的方法，其包括将来自另一物种的更有活性同源物的氨基酸序列与人激素进行比较，以及从所述取代的人类激素中选择超活性类似物。 本发明还涉及编码修饰的人类糖蛋白激素的核酸，含有这些核酸的载体和含有这些载体的宿主细胞。

公开(公告)号：US06361992B1

公开(公告)日：2002-03-26

申请号：US09185408

申请日：1998-11-03

Applicant: Mariusz W. Szkudlinski ,  Bruce D. Weintraub ,  Mathis Grossmann

Inventor： Mariusz W. Szkudlinski ,  Bruce D. Weintraub ,  Mathis Grossmann

IPC: C07K1459

CPC classification number: C07K14/59 ,  A61K38/00 ,  Y10S530/85

Abstract: The invention is directed toward a human glycoprotein hormone having at least one, two, three, four, or five basic amino acids in the &agr;-subunit at positions selected from the group consisting of positions 11, 13, 14, 16, 17, and 20. The invention is also directed to a human glycoprotein where at least one of the amino acids at position 58, 63, and 69 of the &bgr;-subunit of the human thyroid stimulating hormone are basic amino acids. The invention is further directed to a modified human glycoprotein hormone having increased activity over a wild-type human glycoprotein hormone, where the modified human glycoprotein comprises a basic amino acid substituted at a position corresponding to the same amino acid position in a non-human glycoprotein hormone having an increased activity over the wild-type human glycoprotein hormone. The invention is also directed to a method of constructing superactive nonchimeric analogs of human hormones comprising comparing the amino acid sequence of a more active homolog from another species to the human hormone, and selecting superactive analogs from the substituted human hormones. The invention is also directed to nucleic acids encoding the modified human glycoprotein hormones, vectors containing those nucleic acids, and host cells containing those vectors.

Abstract translation: 本发明涉及在α亚基中具有至少一个，二个，三个，四个或五个碱性氨基酸的人类糖蛋白激素，所述碱基氨基酸位于选自下组的位置：第11,13,14,16,17和 本发明还涉及人类糖蛋白，其中人类甲状腺刺激激素的β-亚基的58,63和69位的至少一个氨基酸是碱性氨基酸。 本发明进一步涉及一种与野生型人类糖蛋白激素相比具有增加的活性的修饰的人类糖蛋白激素，其中修饰的人类糖蛋白包含在非人类糖蛋白中与相同氨基酸位置相应位置取代的碱性氨基酸 具有比野生型人类糖蛋白激素增加的活性的激素。 本发明还涉及一种构建人类激素的超活性非嵌段类似物的方法，其包括将来自另一物种的更有活性同源物的氨基酸序列与人激素进行比较，以及从所述取代的人类激素中选择超活性类似物。 本发明还涉及编码修饰的人类糖蛋白激素的核酸，含有这些核酸的载体和含有这些载体的宿主细胞。

公开(公告)号：US20020035060A1

公开(公告)日：2002-03-21

申请号：US08987505

申请日：1997-12-09

Inventor： STEVEN R. PATIERNO ,  MICHAEL J. MANYAK

IPC: A01N037/18 ,  A61K038/00 ,  A61K007/46 ,  A61K039/395 ,  C07K001/00 ,  C07K014/00 ,  C07K017/00 ,  C07K016/00 ,  A61K038/16 ,  A61K035/14

CPC classification number: A61K38/1709 ,  A61K31/405 ,  A61K31/473 ,  C07K14/4721 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  G01N33/5017 ,  G01N33/574 ,  G01N33/57434 ,  Y10S530/85 ,  Y10S530/851 ,  Y10S530/853

Abstract: The present invention relates to pharmaceutical compositions, methods and kits that provide for the early diagnosis and treatment of lung cancer. More particularly, the present invention relates to pharmaceutical compositions containing uteroglobin for preventing or inhibiting metastasis of lung tumor cells and methods of using the same to prevent or inhibit metastasis of lung tumor cells. The present invention also relates to methods and kits for early diagnosis of metastatic lung cancer by assaying for uteroglobin and comparing the results against control cells. The present invention also relates to methods and kits for detection of metastatic lung cancer by assaying for the presence of an aberrant form of uteroglobin.

Abstract translation: 本发明涉及提供肺癌早期诊断和治疗的药物组合物，方法和试剂盒。 更具体地，本发明涉及含有用于预防或抑制肺肿瘤细胞转移的子宫红素的药物组合物及其用于预防或抑制肺肿瘤细胞转移的方法。 本发明还涉及通过测定血红蛋白并将结果与​​对照细胞进行比较来早期诊断转移性肺癌的方法和试剂盒。 本发明还涉及通过测定异常形式的子宫肌钙蛋白的存在来检测转移性肺癌的方法和试剂盒。

公开(公告)号：US5932700A

公开(公告)日：1999-08-03

申请号：US920872

申请日：1997-08-29

Applicant: Atsushi Imai

Inventor： Atsushi Imai

IPC: G01N33/53 ,  A61K38/00 ,  A61P35/02 ,  C07K14/47 ,  C07K14/475 ,  C07K14/52 ,  C07K16/00 ,  C07K16/18 ,  C12N5/10 ,  C12P21/00 ,  C12R1/91 ,  G01N33/574 ,  C07K17/00 ,  C12P21/04 ,  G01N33/48

CPC classification number: C07K14/4703 ,  C07K14/475 ,  A61K38/00 ,  Y10S435/948 ,  Y10S530/85

Abstract: A novel cell growth-inhibiting protein is provided. The cell growth-inhibiting protein, obtainable from extract of human uterine endometrial carcinoma, has the amino acid sequence rich in hydrophobic residue at its N-terminal, has a molecular weight of about 68,000 dalton, and is considered to act as a paracrine growth-inhibiting factor in an organism.

Abstract translation: 提供了一种新的细胞生长抑制蛋白。 从人子宫内膜癌的提取物获得的细胞生长抑制蛋白具有在其N-末端富含疏水性残基的氨基酸序列，分子量约68,000道尔顿，被认为是旁分泌生长抑制蛋白， 抑制因子。

公开(公告)号：US5910568A

公开(公告)日：1999-06-08

申请号：US584671

申请日：1996-01-11

Applicant: Roy H. Hammerstedt ,  Palmer G. Cramer ,  Guy F. Barbato

Inventor： Roy H. Hammerstedt ,  Palmer G. Cramer ,  Guy F. Barbato

IPC: G01N33/50 ,  A61K38/00 ,  A61P15/00 ,  A61P15/08 ,  C07K7/08 ,  C07K14/47 ,  C07K14/705 ,  G01N33/53 ,  C07K14/00 ,  A61K38/16

CPC classification number: C07K14/47 ,  C07K14/705 ,  A61K38/00 ,  Y10S530/827 ,  Y10S530/85 ,  Y10S530/851 ,  Y10S530/852 ,  Y10S530/853

Abstract: A purified polypeptide which provides for initial binding of sperm to oocyte investments and has an active amino acid sequence of SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-Asn -Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro -Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys); or SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Leu -Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly-Pro-Arg-Ser-Lys-Pro-Gln -Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys); or the shorter but biologically active SEQ ID NO:1 and SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, where X is Thr or Pro). The polypeptide is useful in improving sperm-egg binding by fresh sperm, restoring sperm-egg binding following a cryopreservation cycle, enhancing fertilizing potential, and producing antibodies to determine fertilizing potential of sperm, determining sperm-binding sites on egg investments, and contraception.

Abstract translation: 提供精子与卵母细胞投入的初始结合并且具有SEQ ID NO：12（Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn）的活性氨基酸序列的纯化多肽 -Gln-Arg-Gln-Leu-Glu-Ser-Asn -Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile -Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys） 或SEQ ID NO：13（Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-Ser-Asn-Lys-Ile -Pro-Glu-Leu-Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly -Pro-Arg-Ser-Lys-Pro-Gln -Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys）; 或更短但生物活性的SEQ ID NO：1和SEQ ID NO：9（Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn，其中X 是Thr或Pro）。 该多肽可用于通过新鲜精子改善精子 - 卵结合，在冷冻保存循环后恢复精子 - 卵结合，增强受精潜能，并产生抗体以确定精子的施肥潜力，确定卵投射中的精子结合位点和避孕。

公开(公告)号：US5846734A

公开(公告)日：1998-12-08

申请号：US485795

申请日：1995-06-07

Applicant: Ginette Serrero

Inventor： Ginette Serrero

IPC: A61K38/00 ,  C07K14/47 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  G01N33/53 ,  C12Q1/02 ,  C12Q1/32

CPC classification number: C07K14/4705 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  A61K38/00 ,  Y10S530/813 ,  Y10S530/83 ,  Y10S530/846 ,  Y10S530/85 ,  Y10S530/853

Abstract: Mammalian adipogenic factors, including purified proteins or glycoproteins, capable of inducing the adipose differentiation of adipogenic cells are disclosed, as are antibodies to such proteins, DNA encoding the proteins and host cells expressing the proteins. A method for determining the susceptibility of a subject to obesity by measuring the levels of one or more adipogenic factors in a biological fluid or tissue extract is also disclosed, as is a method for evaluating an anti-obesity drug which comprises contacting the drug with cells capable of producing one or more adipogenic factors and measuring the amount of the factors produced.

公开(公告)号：US5831035A

公开(公告)日：1998-11-03

申请号：US328905

申请日：1994-10-25

Applicant: Kathy L. Timms

Inventor： Kathy L. Timms

IPC: G01N33/68 ,  C07K16/18 ,  G01N33/53

CPC classification number: G01N33/689 ,  G01N2800/36 ,  Y10S530/85

Abstract: An antibody to the purified and isolated glycoprotein and functional analogs thereof characterized by being progesterone induced and estradiol inhibited secretory glycoprotein specifically from stromal cells of endometrial origin. The protein has the molecular weight of 70,000 daltons as determined by two-dimensional SDS-PAGE polyacrylamide gel electrophoresis and has an isoelectric point of 5.7.

Abstract translation: 纯化和分离的糖蛋白及其功能类似物的抗体，其特征在于孕酮诱导和雌二醇特异性地从子宫内膜起源的基质细胞中抑制分泌糖蛋白。 该蛋白质通过二维SDS-PAGE聚丙烯酰胺凝胶电泳测定，其分子量为70,000道尔顿，等电点为5.7。