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    Purification of cinnamoyl-C-glycoside chromone
    1.
    再颁专利
    Purification of cinnamoyl-C-glycoside chromone 有权
    肉桂酰-C糖苷色酮的纯化

    公开(公告)号：USRE37771E1

    公开(公告)日：2002-06-25

    申请号：US09414478

    申请日：1999-10-07

    申请人： Todd Waller ,  Qi Jia ,  Abeysinghe Padmapriya

    发明人： Todd Waller ,  Qi Jia ,  Abeysinghe Padmapriya

    IPC分类号： C07H108

    CPC分类号： C07H1/08

    摘要： The present invention provides a process for purifying the cinnamoyl-C-glycoside, 8-C-&bgr;-D-[2-O-(E)-cinnamoyl]glycopyranosyl-2- [(R)-2-hydroxy]propyl-7-methoxy-5-methyl-chromone. 8-C-&bgr;-D-[2′-O- (E)-cinnamoyl]glycopyranosyl-2-[(S) -2-hydroxy]propyl-7-methoxy-5-methylchromone, referred to herein as the “540 compound.” In one embodiment of the present invention the 540 compound is purified by extraction from a decolorizing agent with an organic solvent. The extracted product can be purified by high pressure liquid chromatography. In a second embodiment crude 540 compound, which has not been treated with a decolorizing agent, is purified by passage over neutral alumina or sephadex.

    摘要翻译： 本发明提供了一种纯化肉桂酰基-C-糖苷的方法，“DEL-S DATE =”20020625“ID =”DEL-S-00001“/> 8-C-β-D- [2-O-（E ） - 肉桂酰基]吡喃葡萄糖基-2 - [（R）-2-羟基]丙基-7-甲氧基-5-甲基 - 色酮 8-C-β-D- [2'-O-（E） - 肉桂酰基]吡喃葡萄糖基-2 - [（S）-2-羟基]丙基-7 - 甲氧基-5-甲基色酮，这里称为“540化合物”的INS-E ID =“INS-S-00001”/>。 在本发明的一个实施方案中，通过用有机溶剂从脱色剂中萃取来提纯540化合物。 提取的产物可以通过高压液相色谱法纯化。 在第二个实施方案中，尚未用脱色剂处理的粗540化合物通过中性氧化铝或sephadex通过而纯化。

    Method of preparing l-arabinose from sugar beet pulp
    3.
    发明授权
    Method of preparing l-arabinose from sugar beet pulp 有权
    从甜菜浆中制备1-阿拉伯糖的方法

    公开(公告)号：US06506897B1

    公开(公告)日：2003-01-14

    申请号：US09486437

    申请日：2000-10-30

    申请人： Juhani Antila ,  Vili Ravanko ,  Pertti Walliander

    发明人： Juhani Antila ,  Vili Ravanko ,  Pertti Walliander

    IPC分类号： C07H108

    CPC分类号： C13K13/007 ,  A23L29/231 ,  C07H1/08 ,  C08B37/0048 ,  C08B37/006

    摘要： The invention relates to a method of preparing crystalline L-arabinose by extraction of sugar beet pulp, from which sugar has been extracted, in a strong alkaline solution, by hydrolysis of the obtained crude araban with a strong acid at an elevated temperature, by neutralization and filtration of the obtained solution, by chromatographic separation of the L-arabinose fraction, by purification of the obtained L-arabinose solution by means of cation and anion exchangers and adsorbent resins, and by recovering the pure L-arabinose as a crystalline product.

    摘要翻译： 本发明涉及一种通过在强碱性溶液中提取糖提取甜菜糖浆，通过在高温下用强酸水解所得粗制阿拉伯糖，通过中和来制备结晶L-阿拉伯糖的方法 并通过利用阳离子和阴离子交换剂和吸附剂树脂纯化得到的L-阿拉伯糖溶液，并通过回收纯L-阿拉伯糖作为结晶产物，通过色谱分离L-阿拉伯糖部分过滤所得溶液。

    Method of isolating mucilaginous polysaccharides and uses thereof
    4.
    发明授权
    Method of isolating mucilaginous polysaccharides and uses thereof 有权
    分离粘液多糖的方法及其应用

    公开(公告)号：US06482942B1

    公开(公告)日：2002-11-19

    申请号：US09481111

    申请日：2000-01-11

    申请人： Natale Vittori

    发明人： Natale Vittori

    IPC分类号： C07H108

    CPC分类号： C08B37/0024 ,  A23L33/105 ,  A23L33/22 ,  A23V2002/00 ,  A61K31/715 ,  A61K31/736 ,  A61K36/06 ,  A61K36/07 ,  A61K36/482 ,  A61K36/68 ,  A61K36/886 ,  A61K36/899 ,  A61K36/8998 ,  C08B37/00 ,  C08B37/0003 ,  A61K2300/00 ,  A23V2250/2102 ,  A23V2250/606 ,  A23V2250/712 ,  A23V2250/708

    摘要： The present invention provides a method of isolating mucilaginous polysaccharides from plants, cereals, cell cultures, or fungi such as mushrooms known to have mucilaginous or protein-bound polysaccharides with desirable biological properties. The mucilaginous polysaccharides present in aqueous solution or tissue extracts are treated with tannins to form a complex which is then separated from the solution. The complex is then treated one or more times with either solvents or other substances in solution to remove the bounded tannins from the complex thereby and releasing the isolated polysaccharide. The polysaccharides prepared according to the present method retain properties that are substantially similar to those of the native polysaccharide as it is found in the respective plant or cell. The polysaccharides thus prepared are used in a variety of products. This process is particularly suitable for isolating acetylated mannose polymers from aloe plants and beta glucans.

    摘要翻译： 本发明提供了一种从植物，谷物，细胞培养物或真菌如已知具有期望生物学特性的具有粘液或蛋白质结合多糖的蘑菇中分离出粘液多糖的方法。 存在于水溶液或组织提取物中的粘液多糖用单宁处理以形成复合物，然后将其与溶液分离。 然后用溶液或溶液中的其它物质处理一次或多次复合物，以从络合物中除去有界的单宁，并释放分离的多糖。 根据本发明方法制备的多糖保留与天然多糖基本相似的性质，如在各自的植物或细胞中所发现的那样。 如此制备的多糖用于多种产品中。 该方法特别适用于从芦荟植物和β-葡聚糖中分离乙酰化的甘露糖聚合物。

    Process for isolating and purifying viruses and sugars from plant sources
    5.
    发明授权
    Process for isolating and purifying viruses and sugars from plant sources 失效
    从植物来源分离和纯化维生素和糖的方法

    公开(公告)号：US06303779B1

    公开(公告)日：2001-10-16

    申请号：US09466422

    申请日：1999-12-17

    申请人： Stephen J. Garger ,  R. Barry Holtz ,  Michael J. McCulloch ,  Thomas H. Turpen

    发明人： Stephen J. Garger ,  R. Barry Holtz ,  Michael J. McCulloch ,  Thomas H. Turpen

    IPC分类号： C07H108

    CPC分类号： C12N15/8258 ,  C07K14/415 ,  C07K16/00 ,  C07K16/16 ,  C07K2317/13 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8242 ,  C12N15/8257 ,  C12N2710/00051 ,  C12N2770/00051 ,  Y10S977/904

    摘要： The present invention features a method for isolating and purifying vitamins and sugars from a plant host which is applicable on a large scale. Moreover, the present invention provides a more efficient method for isolating vitamins and sugars than those methods described in the prior art. In general, the present method of isolating vitamins and sugars comprises the steps of homogenizing a plant to produce a green juice, adjusting the pH of and heating the green juice, separating the target species, either vitamins or sugars, from other components of the green juice by one or more cycles of centrifugation, resuspension, and ultrafiltration, and finally purifying vitamins or sugars by such procedure as PEG-precipitation, chromatography and/or salt precipitation.

    摘要翻译： 本发明的特征在于一种从植物宿主中分离和纯化维生素和糖的方法，该方法可大规模应用。 此外，本发明提供了比现有技术中描述的方法更有效的分离维生素和糖的方法。 通常，本发明的分离维生素和糖的方法包括以下步骤：将植物均质化以产生绿汁，调节绿汁的pH并加热绿汁，将目标物种（维生素或糖）与绿色其他成分分离 通过一个或多个离心循环，再悬浮和超滤，最后通过PEG-沉淀，色谱和/或盐沉淀的方法纯化维生素或糖。

    Method for preparing small particle size glucan in a dry material
    6.
    发明授权
    Method for preparing small particle size glucan in a dry material 有权
    在干燥材料中制备小粒径葡聚糖的方法

    公开(公告)号：US06476003B1

    公开(公告)日：2002-11-05

    申请号：US09707583

    申请日：2000-11-06

    申请人： Frank M. Jordan ,  Ruth A. Gault ,  Kenneth W. Hunter

    发明人： Frank M. Jordan ,  Ruth A. Gault ,  Kenneth W. Hunter

    IPC分类号： C07H108

    CPC分类号： A61K31/716

    摘要： An improved method for purifying glucan to small particle size glucan and drying the glucan to a solid such that the glucan may be re-hydrated and maintain substantially maintain a particle size of one micron or less so that it may be used in nutritional, pharmaceutical and pharmacological compositions where a dry material is desired such that a greater immunological benefit may be obtained.

    摘要翻译： 一种用于将葡聚糖纯化成小粒度葡聚糖并将葡聚糖干燥成固体的改进方法，使得葡聚糖可以被再水合并保持基本上保持1微米或更小的颗粒尺寸，使得其可用于营养，药物和 需要干燥材料以获得更大的免疫学益处的药物组合物。

    Process for the crystallization of lactitol
    7.
    发明授权
    Process for the crystallization of lactitol 有权
    乳糖醇结晶的方法

    公开(公告)号：US06407227B1

    公开(公告)日：2002-06-18

    申请号：US09380434

    申请日：1999-12-06

    申请人： Juha Nurmi ,  Miika Kaira

    发明人： Juha Nurmi ,  Miika Kaira

    IPC分类号： C07H108

    CPC分类号： C07H15/04

    摘要： The present invention relates to a process for the production of structurally pure lactitol crystal forms selected from the group consisting of anhydrous lactitol, lactitol monohydrate, lactitol dihydrate and lactitol trihydrate. The crystallization is performed by cooling a lactitol solution from a temperature at or slightly below the highest temperature of the stability area of the respective crystalline lactitol form to a temperature at or slightly above the lowest temperature of the stability area of said crystalline lactitol form, said stability areas being defined, respectively, within the temperature limits of 100° C. and 0° C. by the intersections of the solubility lines shown in FIG. 1, and by maintaining the supersaturation of said lactitol solution at a level of 1 to 8% (w/w) above the solubility line of the respective lactitol form crystallizing in said area.

    摘要翻译： 本发明涉及一种制备选自无水乳糖醇，乳糖醇一水合物，乳糖醇二水合物和乳糖醇三水合物的结构纯的乳糖醇晶体形式的方法。 通过将乳糖醇溶液从相应的结晶乳糖醇形式的稳定区域的最高温度或稍低于所述结晶乳糖醇形式的稳定区域的最高温度的温度冷却至所述结晶乳糖醇形式的稳定区域的最低温度或稍高于所述结晶乳糖醇形式的稳定区域的温度来进行结晶，所述 稳定区域分别在图1所示的溶解度线的交点处于100℃和0℃的温度范围内。 并且通过将所述乳糖醇溶液的过饱和度保持在高于所述区域中结晶的各个乳糖醇形式的溶解度线上1至8％（w / w）的水平。

    Method for isolating DNA
    8.
    发明授权
    Method for isolating DNA 失效
    分离DNA的方法

    公开(公告)号：US06342387B1

    公开(公告)日：2002-01-29

    申请号：US09508119

    申请日：2000-06-12

    申请人： Yoshihide Hayashizaki ,  Piero Carninci

    发明人： Yoshihide Hayashizaki ,  Piero Carninci

    IPC分类号： C07H108

    CPC分类号： C12N15/1017 ,  C12N15/1006

    摘要： A method for isolating DNA contained in a biological sample, including: lysing a DNA-containing biological sample and forming a DNA-bound carrier by placing a lysing solution, including the DNA-containing biological sample, a salt, and a cationic surfactant, and having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, into contact with a DNA-binding carrier to bind DNA to the DNA-binding carrier to form the DNA-bound carrier; separating the DNA-bound carrier from other components; dissociating the bound DNA from the DNA-binding carrier; and recovering dissociated DNA. By the method, DNA purified with no preliminary treatment of a biological sample can be recovered at a high yield.

    摘要翻译： 一种用于分离生物样品中包含的DNA的方法，包括：通过放置含有DNA的生物样品，盐和阳离子表面活性剂的裂解溶液来裂解含DNA的生物样品并形成DNA结合的载体，以及 具有高于DNA沉淀抑制引发浓度的盐浓度与DNA结合载体接触以将DNA结合到DNA结合载体上以形成DNA结合的载体; 将DNA结合的载体与其他成分分离; 从DNA结合载体解离结合的DNA; 并回收解离的DNA。 通过该方法，可以以高收率回收不经过生物样品的预处理纯化的DNA。