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公开(公告)号：US20140378675A1
公开(公告)日：2014-12-25
申请号：US14479198
申请日：2014-09-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6818 , C12Q1/6837 , C12Q2525/185 , C12Q2525/301 , C12Q2565/107 , C12Q2565/101 , C12Q2525/101
摘要： The invention provides novel oligonucleotides and methods of using the same for detection or measurement of specific nucleic acid molecules. The invention also features nucleic acid arrays comprising the oligonucleotides of the invention. An oligonucleotide of the invention comprises (1) a reporter-binding sequence capable of hybridizing to a fluorophore-labeled reporter sequence and (2) a hairpin-forming sequence capable of forming a stem-loop. Formation of the stem-loop modifies (e.g., quenching) the fluorescence signals of the reporter sequence when the reporter sequence is hybridized to the oligonucleotide. This can be achieved, for example, by bringing one or more guanine based in the oligonucleotide into close proximity to the fluorophore(s) of the reporter sequence by virtue of the formation of the stem-loop. Disruption of the stem-loop, such as by hybridization of a target sequence to at least part of the hairpin-forming sequence, produces a detectable change in the fluorescence signals.
摘要翻译： 本发明提供新的寡核苷酸及其用于检测或测量特异性核酸分子的方法。 本发明还具有包含本发明寡核苷酸的核酸阵列。 本发明的寡核苷酸包含（1）能够与荧光团标记的报告基因序列杂交的报告基因结合序列和（2）能够形成茎环的发夹形成序列。 当报告物序列与寡核苷酸杂交时，茎环的形成修饰（例如，淬灭）报告基因序列的荧光信号。 这可以通过例如通过使一个或多个基于寡核苷酸的鸟嘌呤通过茎 - 环的形成使报告物序列的荧光团靠近来实现。 茎环的破坏，例如通过目标序列与形成发夹的序列的至少部分的杂交产生荧光信号的可检测的变化。
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公开(公告)号：US4954449A
公开(公告)日：1990-09-04
申请号：US155437
申请日：1988-02-12
IPC分类号： A61K38/00 , C07K16/12 , G01N33/569
CPC分类号： C07K16/12 , G01N33/56911 , A61K38/00 , Y10S424/831
摘要： A human monoclonal antibody produced by a self-reproducing carrier cell, antibody being reactive with PRP capsular polysaccharide. Also disclosed is a process of preparing the antibody from the carrier cell, which is conveniently a hybridoma. Additionally, diagnostic, prophylactic and therapeutic compositions and methods employing the antibody are disclosed. Moreover, a laboratory reagent containing the antibody is described.
摘要翻译： 由自体再生载体细胞产生的人单克隆抗体，该抗体与PRP荚膜多糖反应。 还公开了从载体细胞制备抗体的方法，其方便地是杂交瘤。 另外，公开了使用抗体的诊断，预防和治疗组合物和方法。 此外，描述了含有该抗体的实验室试剂。
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公开(公告)号：US4744982A
公开(公告)日：1988-05-17
申请号：US411115
申请日：1982-08-24
IPC分类号： G01N33/569 , A61K38/00 , A61K39/395 , C07K14/005 , C07K14/195 , C07K14/705 , C07K16/00 , C07K16/12 , C12N5/10 , C12N15/02 , C12P21/08 , C12Q1/02 , A61K39/40 , C12N15/00 , G01N33/53
摘要： A human monoclonal antibody produced by a self-reproducing carrier cell, the antibody being reactive with PRP capsular polysaccharide. Also disclosed is a process of preparing the antibody from the carrier cell, which is conveniently a hybridoma. Additionally, diagnostic, prophylactic and therapeutic compositions and methods employing the antibody are disclosed. Moreover, a laboratory reagent containing the antibody is described.
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公开(公告)号：US20080293580A1
公开(公告)日：2008-11-27
申请号：US10578248
申请日：2004-11-08
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6818 , C12Q1/6837 , C12Q2525/185 , C12Q2525/301 , C12Q2565/107 , C12Q2565/101 , C12Q2525/101
摘要： The invention provides novel oligonucleotides and methods of using the same for detection or measurement of specific nucleic acid molecules. The invention also features nucleic acid arrays comprising the oligonucleotides of the invention. An oligonucleotide of the invention comprises (1) a reporter-binding sequence capable of hybridizing to a fluorrophore-labeled reporter sequence and (2) a hairpin-forming sequence capable of forming a stem-loop. Formation of the stem-loop modifies (e.g., quenching) the fluorescence signals of the reporter sequence when the reporter sequence is hybridized to the oligonucleotide. This can be achieved, for example, by bringing one or more guanine based in the oligonucleotide into close proximity to the fluorophore(s) of the reporter sequence by virtue of the formation of the stem-loop. Disruption of the stem-loop, such as by hybridization of a target sequence to at least part of the hairpin-forming sequence, produces a detectable change in the fluorescence signals.
摘要翻译： 本发明提供新的寡核苷酸及其用于检测或测量特异性核酸分子的方法。 本发明还具有包含本发明寡核苷酸的核酸阵列。 本发明的寡核苷酸包含（1）能够与荧光标记的报道基因序列杂交的报告基因结合序列和（2）能够形成茎环的发夹形成序列。 当报告物序列与寡核苷酸杂交时，茎环的形成修饰（例如，淬灭）报告基因序列的荧光信号。 这可以通过例如通过使一个或多个基于寡核苷酸的鸟嘌呤通过茎 - 环的形成使报告物序列的荧光团靠近来实现。 茎环的破坏，例如通过目标序列与形成发夹的序列的至少部分的杂交产生荧光信号的可检测的变化。
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公开(公告)号：US5599419A
公开(公告)日：1997-02-04
申请号：US472180
申请日：1995-06-07
申请人： Kenneth W. Hunter , Gerald Paoletti
发明人： Kenneth W. Hunter , Gerald Paoletti
CPC分类号： B29C66/8242 , B29C65/2076 , B29C66/1162 , B29C66/52431 , B29C66/81431 , B29C66/8762 , B29L2012/00 , B29L2031/003 , Y10T156/1744
摘要： A method and apparatus for joining plastic profile members together, particularly to form picture frames. The profile members are held in place by fixture members against a locator member. The locator member is withdrawn and a heated blade positioned between the ends of the profile members. The profile members are moved against the thin blade and the end surfaces are heated to an elevated temperature. The blade is removed and the ends are welded together. During a cooling period, the retracted blade can be cleaned as desired by a blade cleaning mechanism. After the joined profiles are removed, the members are returned to positions to begin another cycle.
摘要翻译： 一种用于将塑料型材构件连接在一起的方法和装置，特别是用于形成相框。 轮廓构件通过固定构件固定在定位构件上。 定位器构件被抽出并且被加热的刀片定位在轮廓构件的端部之间。 轮廓构件抵靠薄刀片移动，端面被加热到升高的温度。 刀片被移除，端部焊接在一起。 在冷却期间，通过刀片清洁机构可以根据需要清洁收回的刀片。 删除连接的配置文件后，会将成员返回到位置，开始另一个循环。
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公开(公告)号：US5114674A
公开(公告)日：1992-05-19
申请号：US44767
申请日：1987-05-01
IPC分类号： G01N27/02 , G01N27/22 , G01N33/543
CPC分类号： G01N33/5438 , G01N27/3276
摘要： An array of molecular chains is added to a dielectric material between two electrodes of a capacitive affinity sensor. Such an array of molecular chains greatly changes dielectric properties between the two electrodes to greatly enhance sensitivity of the sensor. In a sensor using direct binding, a viral fragment is bound to the sensor's surface. A molecular chain, comprising an anti-viral antibody, an anti-human antibody, and a protein molecule, binds to the viral fragment. In a sensor using competitive binding a hapten is bound to the sensor's surface. A molecular chain, comprising an antibody with attached aliphatic hydrocarbons, binds to the hapten. A free analyte competes with the hapten to bind with the antibody.
摘要翻译： 分子链阵列被添加到电容式亲和度传感器的两个电极之间的电介质材料。 这种分子链阵列极大地改变了两个电极之间的介电特性，从而大大提高了传感器的灵敏度。 在使用直接结合的传感器中，病毒片段与传感器的表面结合。 包含抗病毒抗体，抗人抗体和蛋白质分子的分子链与病毒片段结合。 在使用竞争性绑定的传感器中，半抗原与传感器的表面结合。 包含具有连接的脂族烃的抗体的分子链与半抗原结合。 游离的分析物与半抗原竞争结合抗体。
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公开(公告)号：US5057598A
公开(公告)日：1991-10-15
申请号：US304884
申请日：1989-02-01
申请人： Matthew Pollack , Kenneth W. Hunter
发明人： Matthew Pollack , Kenneth W. Hunter
IPC分类号： A61K38/00 , C07K16/12 , G01N33/569
CPC分类号： C07K16/1203 , G01N33/56911 , A61K38/00 , G01N2400/50 , Y10S424/803 , Y10S530/806 , Y10S530/808 , Y10S530/825
摘要： Novel monoclonal antibodies are disclosed, the production of which is specified by particular genes contained, conveniently, in biologically pure cultures of self-reproducing carrier cells, such as, but not limited to, ATCC HB 8297 and ATCC HB 8298, such antibodies being reactive with at least part of endotoxin core of Gram-negative bacteria. Processes of prepaU.S. GOVERNMENT RIGHTSThe invention described herein may be manufactured, used and licensed by or for the U.S. Government for governmental purposes only without the payment to the inventors of any royalties thereon.
摘要翻译： 公开了新的单克隆抗体，其生产由特定的基因所指定，方便地在自我繁殖载体细胞的生物纯纯培养物中包括但不限于ATCC HB 8297和ATCC HB 8298，所述抗体是反应性的 至少部分革兰氏阴性细菌的内毒素核心。 公开了制备自体繁殖载体细胞的方法，例如ATCC HB 8297和ATCC HB 8298。 还公开了从载体细胞制备抗体的方法，其方便地是细胞系如杂交瘤。 进一步公开的是使用这种抗体的免疫诊断和免疫检测组合物和方法。 还进一步公开了使用抗体的免疫预防和免疫治疗组合物和方法。 还公开了使用抗体的研究组合物和方法。 并且甚至进一步公开了利用该抗体的组合物和方法，其可用于从含有它的溶液或混合物中纯化或除去内毒素。
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公开(公告)号：US20140106983A1
公开(公告)日：2014-04-17
申请号：US14105944
申请日：2013-12-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6818 , C12Q1/6837 , C12Q2525/185 , C12Q2525/301 , C12Q2565/107 , C12Q2565/101 , C12Q2525/101
摘要： The invention provides novel oligonucleotides and methods of using the same for detection or measurement of specific nucleic acid molecules. The invention also features nucleic acid arrays comprising the oligonucleotides of the invention. An oligonucleotide of the invention comprises (1) a reporter-binding sequence capable of hybridizing to a fluorrophore-labeled reporter sequence and (2) a hairpin-forming sequence capable of forming a stem-loop. Formation of the stem-loop modifies (e.g., quenching) the fluorescence signals of the reporter sequence when the reporter sequence is hybridized to the oligonucleotide. This can be achieved, for example, by bringing one or more guanine based in the oligonucleotide into close proximity to the fluorophore(s) of the reporter sequence by virtue of the formation of the stem-loop. Disruption of the stem-loop, such as by hybridization of a target sequence to at least part of the hairpin-forming sequence, produces a detectable change in the fluorescence signals.
摘要翻译： 本发明提供新的寡核苷酸及其用于检测或测量特异性核酸分子的方法。 本发明还具有包含本发明寡核苷酸的核酸阵列。 本发明的寡核苷酸包含（1）能够与荧光标记的报道基因序列杂交的报告基因结合序列和（2）能够形成茎环的发夹形成序列。 当报告物序列与寡核苷酸杂交时，茎环的形成修饰（例如，淬灭）报告基因序列的荧光信号。 这可以通过例如通过使一个或多个基于寡核苷酸的鸟嘌呤通过茎 - 环的形成使报告物序列的荧光团靠近来实现。 茎环的破坏，例如通过目标序列与形成发夹的序列的至少部分的杂交产生荧光信号的可检测的变化。
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公开(公告)号：US06476003B1
公开(公告)日：2002-11-05
申请号：US09707583
申请日：2000-11-06
IPC分类号： C07H108
CPC分类号： A61K31/716
摘要： An improved method for purifying glucan to small particle size glucan and drying the glucan to a solid such that the glucan may be re-hydrated and maintain substantially maintain a particle size of one micron or less so that it may be used in nutritional, pharmaceutical and pharmacological compositions where a dry material is desired such that a greater immunological benefit may be obtained.
摘要翻译： 一种用于将葡聚糖纯化成小粒度葡聚糖并将葡聚糖干燥成固体的改进方法，使得葡聚糖可以被再水合并保持基本上保持1微米或更小的颗粒尺寸，使得其可用于营养，药物和 需要干燥材料以获得更大的免疫学益处的药物组合物。
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公开(公告)号：US5584960A
公开(公告)日：1996-12-17
申请号：US299569
申请日：1994-09-01
申请人： Kenneth W. Hunter , Gerald Paoletti
发明人： Kenneth W. Hunter , Gerald Paoletti
CPC分类号： B29C66/8242 , B29C65/2076 , B29C66/1162 , B29C66/52431 , B29C66/81431 , B29C66/8762 , B29L2012/00 , B29L2031/003 , Y10T156/1744
摘要： A method and apparatus for joining plastic profile members together, particularly to form picture frames. The profile members are held in place by fixture members against a locator member. The locator member is withdrawn and a heated blade positioned between the ends of the profile members. The profile members are moved against the thin blade and the end surfaces are heated to an elevated temperature. The blade is removed and the ends are welded together. During a cooling period, the retracted blade can be cleaned as desired by a blade cleaning mechanism. After the joined profiles are removed, the members are returned to positions to begin another cycle.
摘要翻译： 一种用于将塑料型材构件连接在一起的方法和装置，特别是用于形成相框。 轮廓构件通过固定构件固定在定位构件上。 定位器构件被抽出并且被加热的刀片定位在轮廓构件的端部之间。 轮廓构件抵靠薄刀片移动，端面被加热到升高的温度。 刀片被移除，端部焊接在一起。 在冷却期间，通过刀片清洁机构可以根据需要清洁收回的刀片。 删除连接的配置文件后，会将成员返回到位置，开始另一个循环。
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