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8 条记录 (耗时 0.025 秒)

    Saccharide compositions, methods and apparatus for their synthesis
    3.
    发明授权
    Saccharide compositions, methods and apparatus for their synthesis 失效
    糖组合物，其合成方法和装置

    公开(公告)号：US06518051B1

    公开(公告)日：2003-02-11

    申请号：US09570236

    申请日：2000-05-12

    申请人： Stephen Roth

    发明人： Stephen Roth

    IPC分类号： C12P702

    CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12N11/00 ,  C12P19/26

    摘要： A method for preparing saccharide compositions is disclosed. The method is reiterative and includes the following three steps. (i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate. (ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit. (iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration.

    摘要翻译： 公开了制备糖组合物的方法。 该方法是重复的并且包括以下三个步骤：（i）通过在有效结合受体部分的条件下将受体部分与怀疑含有糖基转移酶的混合物接触来分离能够将预选的糖单元转移到受体部分的糖基转移酶 和糖基转移酶，从而分离糖基转移酶。 受体部分是蛋白质，糖蛋白，脂质，糖脂或碳水化合物;（ii）然后将分离的糖基转移酶用于催化受体部分与预选糖单元之间的键（iii）步骤（i） 和（ii）在该方法的第一次迭代中获得的中间产物用作第二次迭代的受体部分多次。

    Hyperthermophilic enzymes for industrial chemical redox reactions: a method for biofuel ethanol production
    5.
    发明授权
    Hyperthermophilic enzymes for industrial chemical redox reactions: a method for biofuel ethanol production 有权
    用于工业化学氧化还原反应的超嗜热酶：一种生物燃料乙醇生产的方法

    公开(公告)号：US06737257B2

    公开(公告)日：2004-05-18

    申请号：US09968151

    申请日：2001-10-01

    申请人： Paul Blum

    发明人： Paul Blum

    IPC分类号： C12P702

    CPC分类号： C12N9/0004 ,  C12P7/06 ,  Y02E50/17

    摘要： The present invention is directed to use of hyperthermophilic enzymes for industrial chemical redox reactions such as ethanol production. The present invention is especially useful for the coupled synthesis and recovery of alcohols whereby recovery of alcohol is simplified.

    摘要翻译： 本发明涉及超嗜热酶用于工业化学氧化还原反应如乙醇生产。 本发明对于醇的偶联合成和回收特别有用，因此简化了醇的回收。

    Process for the biological production of 1,3-propanediol with high titer
    7.
    发明授权
    Process for the biological production of 1,3-propanediol with high titer 有权

    公开(公告)号：US06514733B1

    公开(公告)日：2003-02-04

    申请号：US09641652

    申请日：2000-08-18

    申请人： Mark Emptage ,  Sharon L. Haynie ,  Lisa A. Laffend ,  Jeff P. Pucci ,  Gregory Whited

    发明人： Mark Emptage ,  Sharon L. Haynie ,  Lisa A. Laffend ,  Jeff P. Pucci ,  Gregory Whited

    IPC分类号： C12P702

    CPC分类号： C12N9/0006 ,  C12N9/1205 ,  C12N9/90 ,  C12N15/52 ,  C12P7/18

    摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

    Hydroperoxide lyases
    8.
    发明授权
    Hydroperoxide lyases 有权
    氢过氧化物裂解酶

    公开(公告)号：US06238898B1

    公开(公告)日：2001-05-29

    申请号：US09418222

    申请日：1999-10-14

    申请人： Alex Häusler ,  Konrad Lerch ,  Andreas Muheim ,  Natasha Silke

    发明人： Alex Häusler ,  Konrad Lerch ,  Andreas Muheim ,  Natasha Silke

    IPC分类号： C12P702

    CPC分类号： C12N9/0004 ,  C11B9/0015 ,  C12N9/88 ,  C12P7/04 ,  C12P7/24

    摘要： The present invention relates to the production of HPO lyase proteins in hosts via recombinant expression of said proteins. Recombinant HPO lyase proteins, DNA sequences encoding these proteins, vectors containing these DNA sequences and hosts containing these vectors are provided, along with methods for recombinantly producing such proteins, DNA sequences, vectors and hosts. Also provided are processes for producing green note compounds.

    摘要翻译： 本发明涉及通过所述蛋白质的重组表达在宿主中产生HPO裂解酶蛋白。 提供重组HPO裂解酶蛋白质，编码这些蛋白质的DNA序列，含有这些DNA序列的载体和含有这些载体的宿主，以及用于重组产生此类蛋白质，DNA序列，载体和宿主的方法。 还提供了用于生产绿色音符化合物的方法。