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148 条记录 (耗时 0.041 秒)

    Stain-directed molecular analysis for cancer prognosis and diagnosis
    1.
    发明申请
    Stain-directed molecular analysis for cancer prognosis and diagnosis 失效
    用于癌症预后和诊断的染色体分子分析

    公开(公告)号：US20040235067A1

    公开(公告)日：2004-11-25

    申请号：US10484420

    申请日：2004-06-16

    发明人： Douglas D Burkett

    IPC分类号： C12Q001/68 ,  G01N033/574 ,  G01N001/30 ,  G01N033/48

    CPC分类号： A61K49/006 ,  C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/156

    摘要： The location at which tissue samples are obtained to determine whether cells exhibit characeristics associated with cell differentiation or cancer by molecular analysis is determined by topically applying to epithelial tissue a dye that selectively stains cancer and precancerous tissue.

    摘要翻译： 通过局部应用于上皮组织，选择性地染色癌症和癌前组织的染料来确定获得组织样品以确定细胞是否显示与细胞分化或癌症相关的特征的位置。

    Oxidized bioprosthetic materials
    3.
    发明申请
    Oxidized bioprosthetic materials 审中-公开
    氧化生物假体材料

    公开(公告)号：US20040180319A1

    公开(公告)日：2004-09-16

    申请号：US10807991

    申请日：2004-03-23

    发明人： Crystal M. Cunanan ,  Lillian J. Quintero ,  Angela de la Fuente ,  Patrice Tremble

    IPC分类号： A01N001/02 ,  G01N001/30 ,  G01N033/48

    CPC分类号： A61L27/3691 ,  A01N1/00 ,  A61L27/3687 ,  A61L2430/40

    摘要： A method for chemical fixation of tissues by exposing the tissue to a chemical fixative agent, under oxidative conditions. The chemical fixative agents useable in this method include aldehydes (e.g., formaldehyde, glutaraldehyde, dialdehyde starch), isocyanates (e.g., hexamethylene diisocyanate) and certain polyepoxy compounds (e.g., DENACOL). The oxidative conditions may be provided by heating of a chemical fixative solution that contains the crosslinking agent, in the presence of room air or oxygen. Alternatively, the oxidative conditions may be provided by adding one or more oxidizing chemicals (e.g., hydrogen peroxide or other peroxides, sodium periodate or other periodates, diisocyanates, halogens, n-bromosuccinimide or other halogenated compounds, permanganates, ozone, chromic acid, sulfuryl chloride, sulfoxides, selenoxides, etc.) to the chemical fixative solution. Alternatively, the oxidative conditions may be provided by irradiation (e.g., alpha, beta, ultraviolet, electron beam, gamma rays) of the fixative solution in the presence of room air or oxygen.

    摘要翻译： 一种通过在氧化条件下将组织暴露于化学固定剂的组织化学固定的方法。 在该方法中可用的化学固定剂包括醛（例如甲醛，戊二醛，二醛淀粉），异氰酸酯（例如六亚甲基二异氰酸酯）和某些多环氧化合物（例如DENACOL）。 氧化条件可以通过在室内空气或氧气的存在下加热含有交联剂的化学固定剂溶液来提供。 或者，氧化条件可以通过加入一种或多种氧化性化学物质（例如过氧化氢或其它过氧化物，高碘酸钠或其它高碘酸盐，二异氰酸酯，卤素，正溴代琥珀酰亚胺或其它卤代化合物，高锰酸盐，臭氧，铬酸，磺酰基 氯化物，亚砜，硒氧化物等）到化学固定液中。 或者，可以在室内空气或氧气的存在下通过固定溶液的照射（例如α，β，紫外线，电子束，γ射线）提供氧化条件。

    Quantitative determination of protein kinase C activation using optophoretic analysis
    4.
    发明申请
    Quantitative determination of protein kinase C activation using optophoretic analysis 审中-公开
    使用荧光染色分析定量测定蛋白激酶C的活化

    公开(公告)号：US20040023310A1

    公开(公告)日：2004-02-05

    申请号：US10326568

    申请日：2002-12-19

    申请人： Genoptix, Inc

    发明人： Ilona Kariv ,  Kristie Lynn Lykstad ,  Thomas D.Y. Chung

    IPC分类号： C12Q001/68 ,  G01N033/53 ,  G01N033/567 ,  G01N001/30 ,  G01N033/48

    CPC分类号： G01N33/5094 ,  B07C5/34 ,  G01N15/1456 ,  G01N30/02 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/505 ,  G01N33/5067 ,  G01N33/5076 ,  G01N33/5091 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2510/00 ,  B01D2015/3895

    摘要： A method for quantitatively determining the level of protein kinase C (PKC) activation in cells in response to exposure to a PKC activating compound using a moving optical gradient including the steps of providing a series of cell samples, exposing the series of cell samples to different concentrations of the PKC activating compound, moving the cells and the optical gradient relative to each other so as to cause displacement of at least some of the cells, measuring the displacement of at least a portion of the displaced cells for each of the different concentrations, generating a dose response curve of the measured displacement as a function of the concentration of the PKC activating compound, and determining the potency of the PKC activating compound from the dose response curve. The method can also be used to determine the relative efficacy of the PKC activating compound as compared to a standard compound.

    摘要翻译： 一种用于定量测定响应于使用移动光学梯度暴露于PKC激活化合物的细胞中蛋白激酶C（PKC）活化水平的方法，包括以下步骤：提供一系列细胞样品，将所述一系列细胞样品暴露于不同的细胞样品 PKC活化化合物的浓度，移动细胞和相对于彼此的光学梯度，以引起至少一些细胞的位移，测量每个不同浓度的至少一部分置换的细胞的位移， 产生作为PKC活化化合物浓度的函数的测量位移的剂量响应曲线，并从剂量响应曲线确定PKC活化化合物的效力。 该方法也可用于确定与标准化合物相比PKC活化化合物的相对功效。

    Plasticized hydrophilic glasses for improved stabilization of biological agents
    5.
    发明申请
    Plasticized hydrophilic glasses for improved stabilization of biological agents 有权
    增塑亲水玻璃，用于改善生物制剂的稳定性

    公开(公告)号：US20040014164A1

    公开(公告)日：2004-01-22

    申请号：US10199061

    申请日：2002-07-22

    发明人： Marcus T. Cicerone ,  Andrew Tellington ,  Landon Trost ,  Alexei Sokolov

    IPC分类号： G01N001/30

    CPC分类号： A61K9/19 ,  A61K47/10 ,  A61K47/20 ,  A61K47/26 ,  A61K47/32 ,  A61K47/36 ,  C12N1/04 ,  C12N9/96

    摘要： The stabilization of biomaterials such as proteins in a nominally dry, hydrophilic glassy matrix is vastly improved by the addition of an appropriate amount of a small-molecule pasticizer such as a glycol or DMSO to the formulation, while maintaining a glass transition temperature (Tg) that is above the storage temperature. By plasticizing the glasses, their ability to preserve proteins is improved by as much as 100 times over the unplasticized glass at room temperature. The plasticizer confers the greatest beneficial effect when it is dynamically coupled into the bulk glass, and this coupling occurs over a fairly narrow range of plasticizer concentration. Methods are described in which a small-molecule plasticizer can be incorporated into a glass made of much larger molecules (e.g. a polymeric glass), with desired dynamic coupling, via a molecule that is believed to act as a dynamic linker. Protein preservation data was obtained from two enzymes, horseradish peroxidase (HRP) and alcohol dehydrogenase (ADH).

    摘要翻译： 通过向制剂中加入适量的小分子塑料剂如二醇或DMSO，生物材料如名义上干燥的亲水性玻璃质基质中的蛋白质的稳定性大大提高，同时保持玻璃化转变温度（Tg） 高于储存温度。 通过塑化玻璃，在室温下，其保存蛋白质的能力比未增塑玻璃提高了100倍。 当增塑剂动态耦合到本体玻璃中时，增塑剂赋予最大的有益效果，并且该耦合发生在相当窄的增塑剂浓度范围上。 描述了一种方法，其中可以通过被认为作为动态接头的分子将小分子增塑剂引入由较大分子（例如聚合物玻璃）制成的玻璃中，并具有所需的动态偶联。 从两种酶，辣根过氧化物酶（HRP）和醇脱氢酶（ADH）获得蛋白质保存数据。

    High-throughput tissue microarray technology and applications
    7.
    发明申请
    High-throughput tissue microarray technology and applications 审中-公开
    高通量组织微阵列技术及应用

    公开(公告)号：US20030215936A1

    公开(公告)日：2003-11-20

    申请号：US10149520

    申请日：2003-04-14

    发明人： Olli Kallioniemi ,  Guido Sauter ,  Stephen B. Leighton ,  Juha Kononen ,  Thomas J. Pohida ,  John William Karareka ,  Ghadi Hamdi Salem

    IPC分类号： C12M001/34 ,  G01N001/30 ,  G01N033/48

    CPC分类号： G01N1/36 ,  G01N1/06 ,  G01N2001/368

    摘要： A method and apparatus are disclosed for a high-throughput, large-scale molecular profiling of tissue specimens by retrieving a donor tissue specimen from an array of donor specimens, placing a sample of the donor specimen in an assigned location in a recipient array, providing substantial copies of the array, performing a different biological analysis of each copy, and storing the results of the analysis. The results may be compared to determine if there are correlations or discrepancies between the results of different biological analyses at each assigned location, and also compared to clinical information about the human patient from which the tissue was obtained. The results of similar analyses on corresponding sections of the array can be used as quality control devices, for example by subjecting the arrays to a single simultaneous investigative procedure. Uniform interpretation of the arrays can be obtained, and compared to interpretations of different observers.

    摘要翻译： 公开了一种方法和装置，用于通过从供体样本阵列中取出供体组织标本，将供体标本的样品放置在受体阵列中的指定位置，提供组织标本的高通量，大规模分子图谱，提供 阵列的大量副本，对每个副本执行不同的生物分析，并存储分析结果。 可以比较结果以确定在每个指定位置处的不同生物分析的结果之间是否存在相关性或差异，并且还与获得组织的人类患者的临床信息进行比较。 对阵列的相应部分进行类似分析的结果可以用作质量控制装置，例如通过对阵列进行单一的同时调查程序。 可以获得阵列的均匀解释，并与不同观察者的解释进行比较。

    Fluorescent compounds
    9.
    发明申请
    Fluorescent compounds 审中-公开
    荧光化合物

    公开(公告)号：US20030157518A1

    公开(公告)日：2003-08-21

    申请号：US10258310

    申请日：2003-02-24

    发明人： Philip John Livingston Bell ,  Peter Karuso

    IPC分类号： C12Q001/68 ,  G01N033/53 ,  C07H021/04 ,  G01N033/48 ,  C12P017/16 ,  G01N001/30 ,  C07D307/91

    CPC分类号： C07D493/04 ,  C09B57/00 ,  C09B61/00 ,  G01N33/582 ,  G01N33/6839

    摘要： Fluorescent dye compounds of formula (I) are disclosed. These compounds are useful as they interact with organic compounds in a manner such that excitation with certain wavelengths of light results in fluorescent emmission. Detection and/or monitoring of the fluorescence provides a means for the detection or quantification of organic compounds when bound to these fluorescent dye compounds. Formula (I), wherein: each of R, Rnull and Rnull is a hydrogen atom, halogen atom or a straight or branched chain (C1-20alkyl, alkenyl or alkynyl group optionally substituted with one or more halogen, hydroxy and/or oxy group: rings A, B and C optionally include one or more double bonds; rings B and C are optionally substituted with one or more halogen atoms.

    摘要翻译： 公开了式（I）的荧光染料化合物。 这些化合物是有用的，因为它们以与某些波长的光的激发导致荧光发射的方式与有机化合物相互作用。 荧光检测和/或监测提供了当与这些荧光染料化合物结合时检测或定量有机化合物的手段。 式（I）其中：R，R'和R“各自为氢原子，卤原子或直链或支链（C 1-20烷基，烯基或炔基，任选被一个或多个卤素，羟基和/ 或氧基：环A，B和C任选地包括一个或多个双键;环B和C任选被一个或多个卤素原子取代。