DNA marking of previously undistinguished items for traceability

    公开(公告)号:US09919512B2

    公开(公告)日:2018-03-20

    申请号:US15049170

    申请日:2016-02-22

    摘要: The invention provides a method of marking an item with naturally-derived or synthetic non-natural polymeric marker molecules, such as a DNA or Peptide marker in conjunction with optional visible or rapid scan reporters for authenticating or tracking, in which the method includes providing an item for marking, and applying a medium including a DNA marker to the item. The invention further provides methods for stably binding and immobilizing activated deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. Also provided are methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. The invention also provides a method of marking an item with a DNA marker for authenticating or tracking, in which the method includes providing a medium including a DNA marker, and molding the medium including the DNA marker to provide all or part of the item. The DNA marker encodes information unique to the item and/or the model of the item as desired.

    DNA marking of previously undistinguished items for traceability
    5.
    发明授权
    DNA marking of previously undistinguished items for traceability 有权
    以前不可区分的物品的DNA标记可追溯性

    公开(公告)号:US09266370B2

    公开(公告)日:2016-02-23

    申请号:US14584100

    申请日:2014-12-29

    摘要: The invention provides a method of marking an item with naturally-derived or synthetic non-natural polymeric marker molecules, such as a DNA or Peptide marker in conjunction with optional visible or rapid scan reporters for authenticating or tracking, in which the method includes providing an item for marking, and applying a medium including a DNA marker to the item. The invention further provides methods for stably binding and immobilizing activated deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. Also provided are methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. The invention also provides a method of marking an item with a DNA marker for authenticating or tracking, in which the method includes providing a medium including a DNA marker, and molding the medium including the DNA marker to provide all or part of the item. The DNA marker encodes information unique to the item and/or the model of the item as desired.

    摘要翻译: 本发明提供了一种使用天然衍生或合成的非天然聚合物标记分子(例如DNA或肽标记)与用于鉴定或跟踪的可选的可见或快速扫描记录器一起标记物品的方法,其中所述方法包括提供 用于标记的项目,以及将包括DNA标记的培养基应用于该物品。 本发明还提供了将活化的脱氧核糖核酸稳定结合并固定在物体和底物上的方法。 该方法包括将脱氧核糖核酸暴露于碱性条件下,并使脱氧核糖核酸与对象或底物接触。 还提供了用于增加标签从对象的可恢复性的方法。 所述方法包括将标签剂掺入溶液中的步骤; 将包含标签剂的溶液与扰动剂混合以形成第一扰动标记剂溶液; 将第一扰动标记剂溶液与聚合物混合以形成第二扰动标记聚合物溶液; 以及将所述第二扰动标记聚合物溶液施加到所述物体的至少一部分以形成标记剂涂覆物体。 本发明还提供了用用于鉴定或追踪的DNA标记物标记物品的方法,其中所述方法包括提供包含DNA标记物的培养基,并且模制包含DNA标记的培养基以提供该物品的全部或部分。 DNA标记根据需要编码项目和/或项目模型唯一的信息。

    Method and composition of matter for detecting large quantities of paper
currency
    7.
    发明授权
    Method and composition of matter for detecting large quantities of paper currency 失效
    用于检测大量纸币的物质的方法和组成

    公开(公告)号:US5057268A

    公开(公告)日:1991-10-15

    申请号:US629994

    申请日:1990-12-19

    申请人: Richard A. Muller

    发明人: Richard A. Muller

    摘要: Paper notes used as a monetary currency are deuterated. The level of deuteration while not complete, is high. For U.S. currency the level of deuteration is at least 0.1 mg of deuterium for each one dollar in value of the currency note, and preferably at least 0.3 mg. Use of X-ray or gamma ray interrogation with a beam energy above 2 MeV produces a nuclear reaction releasing a neutron from the deuterium nucleus. If the currency is in large concentrations, e.g. $100,000 or more, the neutrons emitted by this reaction are reliably detectable. The deuteration occurs in the cellulose fibers forming the currency. To resist an exchange of hydrogen atoms for deterium atoms, the deuterium atoms can be used in the formation of synthetic cellulose where the deuterium is more deeply buried within the cellulose molecule than in naturally occurring cellulose. The deuterated synthetic fibers are blended with natural, non-deuterated fibers to form the paper. The currency can also include a mechanism, such as dye, to signal attempts to use solvents or otherwise facilitate any such hydrogen substitution.

    摘要翻译: 用作货币的纸币是氘化的。 氘代水平不完全高。 对于美国货币,贬值水平对于货币价值的每一美元至少为0.1毫克氘,优选至少为0.3毫克。 使用X射线或γ射线询问,光束能量高于2 MeV会产生从氘核释放中子的核反应。 如果货币是大量的,例如 10万美元以上,这种反应发射的中子是可靠的检测。 在形成货币的纤维素纤维中发生氘化。 为了阻止氢原子交换用于腐蚀原子,氘原子可用于合成纤维素的形成,其中氘比纤维素中天然纤维素分子更深地埋在其中。 氘代合成纤维与天然的非氘化纤维混合形成纸。 该货币还可以包括诸如染料之类的机制来表明尝试使用溶剂或以其他方式促进任何这种氢取代。

    Scratch card, and method and apparatus for validation of the same
    9.
    发明授权
    Scratch card, and method and apparatus for validation of the same 失效
    刮卡及其验证方法和装置

    公开(公告)号:US6047964A

    公开(公告)日:2000-04-11

    申请号:US52657

    申请日:1998-03-31

    摘要: An improved scratch card instant lottery ticket includes micro-encapsulated chemical reactants which, when released, irreversibly form one of a visual color change or a fluorescence signature at a location of the card. Both the visual color change and fluorescence signature indicate that the location has been played. Scratch cards are also marked to indicate that they have been read. Cards are marked by either automatically activating chemical reactants to form a visual color and a fluorescence signature, heating a thermofluorescent material to alter a fluorescence signature, or applying a heat-responsive material to the scratch card in such that when the identification code is read, an altered material is detected. Also taught are a method and apparatus for evaluating the scratch card to determine which locations on the card have been played. The evaluation method includes the steps of: (A) directing over at least two angles a beam of light emitted from a light source to impinge on a location of the card; (B) detecting for each of the at least two angles a component of the beam of light as it leaves the location; (C) measuring scattering angles for the location from the components detected leaving the location over the at least two angles; and (D) comparing the scattering angles of the location to a predetermined threshold, and when the angles exceed the threshold identifying the location as unplayed.

    摘要翻译: 改进的刮刮卡即时彩票包括微胶囊化学反应物,其在释放时不可逆地形成卡的位置处的视觉颜色变化或荧光特征之一。 视觉颜色变化和荧光签名均表明该位置已被播放。 刮卡也被标记为表示它们已被读取。 卡被自动激活化学反应物以形成视觉颜色和荧光特征,加热热荧光材料以改变荧光特征,或者将热响应材料施加到刮刮卡上,使得当识别码被读取时, 检测到改变的材料。 还教导了用于评估刮刮卡以确定卡上的哪些位置已被播放的方法和装置。 评估方法包括以下步骤:(A)从光源发射的光束至少两个角度引导到卡的位置; (B)当所述光束离开所述位置时,检测所述至少两个角度中的每个角度; (C)从所检测到的部件离开所述位置测量位于所述至少两个角度上的位置的散射角; 以及(D)将所述位置的散射角与预定阈值进行比较,以及当所述角度超过所述阈值时,将所述位置识别为未被播放。

    Verification method using pressure and heat-sensitive chromogenic system
    10.
    发明授权
    Verification method using pressure and heat-sensitive chromogenic system 失效
    使用压敏和感热显色系统的验证方法

    公开(公告)号:US5595955A

    公开(公告)日:1997-01-21

    申请号:US341159

    申请日:1994-11-16

    摘要: A pressure and heat-sensitive composition comprising chromogen-containing pressure-rupturable microcapsules and heat-sensitive color developer useful for verification of document authenticity system when applied as a localized coating on documents, such as checks and prescriptions. Quickly striking the chromogenic composition with a fingernail or blunt object to generate pressure and heat in the coating produces a colored image by friction. The chromogenic composition possesses a fugitive characteristic, whereby the colored image gradually disappears and can be made to reappear when the chromogenic coating is again subjected to external pressure and heat. This feature can be used for repeated verification of the authenticity of the document.

    摘要翻译: 一种压敏和热敏组合物,其包含含色原体的压力可破裂微胶囊和热敏彩色显影剂,当用作文件上的局部涂层(例如检查和处方)时,可用于验证文件真实性系统。 用指甲或钝物快速地击发发色组合物以在涂层中产生压力和热量,通过摩擦产生着色图像。 发色组合物具有短暂的特性,因此,着色图像逐渐消失,并且当显色涂层再次经受外部压力和热量时可以重新出现。 此功能可用于重复验证文档的真实性。