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公开(公告)号:US20250051702A1
公开(公告)日:2025-02-13
申请号:US18930047
申请日:2024-10-29
Applicant: SHIMOSE MICROBES LABORATORY CORPORATION
Inventor: Shinichi SHIMOSE
Abstract: Provided is an energy-saving, environmentally friendly biogas generation system that can reduce the generation of digestive fluid, can efficiently cause methane fermentation by removing ammonia in organic matter regardless of the freshness of the organic matter. The biogas generation system includes a sealed container 2 that contains the organic matter, a stirrer 3 that stirs the organic matter contained in the sealed container 2, a gas-fired boiler 4 that heats the inside of the sealed container 2 to a predetermined temperature, a vacuum pump 5 that reduces pressure in the sealed container 2, and a storage tank 6 that stores biogas generated by methane fermentation of the organic matter in the sealed container 2. Part of the biogas stored in the storage tank 6 is supplied to the gas-fired boiler 4 as fuel.
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公开(公告)号:US12221643B2
公开(公告)日:2025-02-11
申请号:US17706569
申请日:2022-03-28
Applicant: Jaron C. Hansen , Lee D Hansen , Zachary T Aanderud , Conly L Hansen
Inventor: Jaron C. Hansen , Lee D Hansen , Zachary T Aanderud , Conly L Hansen
Abstract: A method for biogas production includes feeding a receiving structure with a feed containing biomass that comprises lignocellulose and directing the feed from the receiving structure into an anaerobic secretome bioreactor (ASB) reactor environment which includes a synthetic microbial community consisting of at least one selected from extremophile thermophilic anaerobic microorganisms that are essentially acidogens and acetogens, the synthetic microbial community producing a secretome of exozymes. The ASB treated biomass is further directed to an aerobic digestion (AD) reactor environment, the ASB treated biomass being pasteurized so as to be essentially free of non-thermophilic microorganisms, the ASB treated biomass comprising liquid effluent containing solubilized biomass products and metabolized biomass products, and solid effluent. Contents or heat are recycled through a conduit between the ASB reactor environment and the AD reactor environment.
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公开(公告)号:US20250034502A1
公开(公告)日:2025-01-30
申请号:US18716760
申请日:2022-12-07
Applicant: EPPENDORF SE
Inventor: Anna Schütt
Abstract: An agitator for a bioreactor includes an agitator shaft having an agitator unit with an agitator blade receiving portion and at least one agitator blade arranged on the agitator blade receiving portion. The at least one agitator blade is movable between a first angular position and a second angular position, wherein the at least one agitator blade is oriented at a first angle of attack in the first angular position and at a second angle of attack in the second angular position.
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公开(公告)号:US20240384215A1
公开(公告)日:2024-11-21
申请号:US18687653
申请日:2022-02-18
Applicant: SHIMADZU CORPORATION
Inventor: Tomohisa HASUNUMA
Abstract: The cell culturing device includes a vessel that accommodates a culture solution containing a medium and cells, an agitator that is provided in the vessel and agitates the culture solution, a discharge pipe that discharges the culture solution in the vessel to outside the vessel, and an oxygen intake pipe that draws oxygen into the vessel. The discharge pipe has an end below the agitator in the vertical direction.
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公开(公告)号:US20240350992A1
公开(公告)日:2024-10-24
申请号:US18652521
申请日:2024-05-01
Applicant: Life Technologies Corporation
Inventor: Nephi D. Jones , Brandon M. Knudsen , Whitt F. Woods
IPC: B01F35/513 , B01F27/07 , B01F27/213 , B01F27/807 , B01F27/88 , B01F27/91 , B01F31/20 , B01F31/23 , B01F35/42 , B01F35/43 , B01F35/71 , B01F35/75 , C12M1/00 , C12M1/02 , C12M1/06 , C12M3/00 , C12M3/06
CPC classification number: B01F35/513 , B01F27/071 , B01F27/213 , B01F27/807 , B01F27/88 , B01F27/91 , B01F31/201 , B01F31/23 , B01F35/42 , B01F35/421 , B01F35/43 , B01F35/712 , B01F35/7548 , C12M23/14 , C12M23/48 , C12M23/52 , C12M27/00 , C12M27/02 , C12M27/16 , C12M29/06 , B01F35/715
Abstract: A method for mixing a fluid includes: dispensing a first volume of a fluid into a flexible container, the flexible container being at least partially disposed within the chamber of a support housing; repeatedly moving the support housing and the flexible container contained therein so as to mix the first volume of fluid within the flexible container; adding further fluid into the flexible container after moving the support housing to form a second volume of fluid; and manipulating a mixing element within the flexible container so as to mix the second volume of fluid.
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公开(公告)号:US12121543B2
公开(公告)日:2024-10-22
申请号:US15773099
申请日:2016-10-31
Inventor: Tomohiro Shigemori , Haruki Okamoto , Yoshikazu Kato
IPC: C12M1/06 , A61K35/19 , A61K35/545 , C12M1/00 , C12M1/02 , C12M1/36 , C12M3/08 , C12N5/10 , C12N5/16 , C12N15/85 , C12M3/00 , C12N15/09
CPC classification number: A61K35/19 , A61K35/545 , C12M1/02 , C12M3/08 , C12M21/00 , C12M27/00 , C12M29/00 , C12M41/48 , C12N5/10 , C12N5/16 , C12N15/85 , C12M3/00 , C12N15/09 , C12N2015/8572
Abstract: The present invention provides a method for producing platelets, including the step of culturing megakaryocyte cells in a culture solution in a culture vessel, wherein in the culturing step, the culture solution is stirred by a stirring blade moving reciprocally.
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公开(公告)号:US20240327778A1
公开(公告)日:2024-10-03
申请号:US18739392
申请日:2024-06-11
Applicant: PBS BIOTECH, INC.
Inventor: Chanyong Brian Lee
CPC classification number: C12M41/48 , C12M23/24 , C12M23/28 , C12M23/34 , C12M27/02 , C12M29/08 , C12M41/26 , C12M41/34
Abstract: Systems and methods for treating media in large-scale bioreactors can include a method of using a large-scale bioreactor system. The method can include (a) mixing a mixture of at least cells, and media in a bioreactor, and (b) removing a portion of the media through an output port of the bioreactor. The method can also include (c) retaining the cells within the bioreactor using a screen covering the port. The method can also include (d) receiving a second media, which may in some examples include the portion of the first media removed from the bioreactor, into a media preparation container external to the bioreactor, (e) treating the second media within the media preparation container to produce oxygenated media within a threshold pH range, and (f) introducing the oxygenated media into the bioreactor using a return port of the bioreactor.
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公开(公告)号:US20240318109A1
公开(公告)日:2024-09-26
申请号:US18575217
申请日:2022-07-15
Inventor: Mark VARLEY , Sean SULLIVAN , Adrian Neil BARGH
Abstract: A consumable (1) for a bioreactor system is provided. The consumable comprises a plurality of bioreaction vessels (2), each suitable for holding the contents of a bioreaction. The plurality of bioreaction vessels are provided as a single-piece construction, and each bioreaction vessel includes an agitator (4) configured to agitate the contents of the respective bioreaction vessel. Each agitator has a stirring end which extends into the bioreaction vessel and a drive end.
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公开(公告)号:US20240294859A1
公开(公告)日:2024-09-05
申请号:US18589775
申请日:2024-02-28
Applicant: PBS BIOTECH, INC.
Inventor: Gary Evans , Sunghoon Jung
Abstract: A multi-level port vessel includes a vessel body defining an interior volume. The vessel body has a front wall, a rear wall, two side walls, a bottom, and a top. The top is open and adapted to be covered by a removable lid. A vertically oriented mixing wheel is disposed within the interior volume. The vertically oriented mixing wheel has a substantially horizontal rotation axis when the vessel body is disposed on a substantially horizontal surface. A plurality of ports is disposed in one of the rear wall, the front wall, or the side walls. At least one port in the plurality of ports is located at an elevation that is at least partially encompassed by a projection of the perimeter of the vertically oriented mixing wheel.
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公开(公告)号:US12076681B2
公开(公告)日:2024-09-03
申请号:US17991589
申请日:2022-11-21
Applicant: Life Technologies Corporation
Inventor: Jason D. Brown , Clinton C. Staheli , Nephi D. Jones
IPC: B01D46/58 , B01D46/00 , B01F23/231 , B01F27/2121 , B01F27/88 , B01F27/90 , B01F35/10 , B01F35/513 , C12M1/00 , C12M1/06 , C12M1/12
CPC classification number: B01D46/58 , B01D46/0005 , B01F23/2312 , B01F27/2121 , B01F27/88 , B01F27/90 , B01F35/146 , B01F35/513 , C12M23/14 , C12M27/02 , C12M29/06 , C12M37/02 , B01F23/231262
Abstract: A method for filtering a gas comprises passing a gas through a compartment of a filter assembly, the filter assembly comprising: an inlet opening; a first outlet opening; a casing comprising polymeric film and bounding the compartment, the compartment communicating with the inlet opening and the first outlet opening; and a first filter at least partially disposed within the compartment. The method further comprising forming a first seal across a first section of the casing at a location between the inlet opening and the first filter to form a first sub-compartment within the casing and severing the casing at a first location.
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