公开(公告)号：WO2008060604A3

公开(公告)日：2009-02-19

申请号：PCT/US2007024022

申请日：2007-11-14

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WILLIAMS JEFF

Inventor： HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WILLIAMS JEFF

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N21/00

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/045 ,  B01L2300/046 ,  B01L2300/0861 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/1861 ,  C12Q1/686 ,  G01N21/6428 ,  G01N2021/6419 ,  Y10S435/808 ,  C12Q2565/629

Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

Abstract translation: 本技术提供了用于检测样品中特别是生物样品中多核苷酸的装置。 该技术更特别涉及在微流体通道内对感兴趣的核苷酸进行PCR并检测这些核苷酸的微流体系统。 该装置包括被配置为接受多个样品并且可以分别对每个样品或一组或多个样品同时进行PCR的微流控盒。

公开(公告)号：WO02078845A9

公开(公告)日：2002-12-12

申请号：PCT/US0209439

申请日：2002-03-27

Applicant: HANDYLAB INC

Inventor： HANDIQUE KALYAN ,  GANESAN KARTHIK ,  BRAHMASANDRA SUNDARESH N

IPC: G01N1/00 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B81B1/00 ,  G01N1/28 ,  G01N37/00 ,  B01D57/02 ,  B01D59/42 ,  B01D59/50 ,  B01D61/42 ,  B01D61/58 ,  B01L3/02 ,  C02F1/40 ,  C02F11/00 ,  G01N1/10 ,  G01N27/26 ,  G01N27/27 ,  G01N27/403 ,  G01N27/453 ,  G01N31/00

CPC classification number: B01L7/52 ,  B01F13/0071 ,  B01F13/0079 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00936 ,  B01J2219/00943 ,  B01J2219/00945 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01J2219/00986 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2300/0816 ,  B01L2400/0442 ,  B01L2400/0493 ,  B01L2400/0677 ,  G01N1/4077 ,  G01N35/1083 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected n the preferred structure of the provided methods and systems. In particular, the methods are structured into low -level device component control functions, middle-level actuator, control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring (reaction/detectiong1), mixing (mixing 1), heating (heater1, heater2), and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations. Advantageously, a single control system can thereby control numerous different reactions in numerous different microfluidic devices simply by loading different easily understood micro-droplet programs.

Abstract translation: 本发明提供了用于微流体装置的控制方法，控制系统和控制软件，所述微流体装置通过将离散的微小液滴移动通过一系列确定的配置来操作。 这种微流体装置优选以分层和模块化的方式构建，其反映在所提供的方法和系统的优选结构上。 具体而言，这些方法被构造成低级设备组件控制功能，中级执行器，控制功能和高级微液滴控制功能。 有利的是，可以通过调用微滴控制功能来指示微流体装置执行预期的反应或分析，所述微滴控制功能执行直观的任务，如测量（反应/检测g1），混合（混合1），加热（加热器1，加热器2）等等 向前。 该系统优选是可编程的并且能够适应由低电压控制并且以标准化配置构建的微流体装置。 有利地，单个控制系统可以由此简单地通过加载不同的容易理解的微滴程序来控制许多不同微流体装置中的许多不同的反应。

公开(公告)号：WO03022442A8

公开(公告)日：2003-10-02

申请号：PCT/US0229012

申请日：2002-09-12

Applicant: HANDYLAB INC ,  GANESAN KARTHIK

Inventor： GANESAN KARTHIK

IPC: G01N35/00 ,  B01J19/00 ,  B01L3/00 ,  B81B1/00 ,  G01N37/00 ,  B01L11/00

CPC classification number: G05D7/0617 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L7/00 ,  B01L2200/02 ,  B01L2200/14 ,  B01L2200/147 ,  B01L2300/02 ,  B01L2300/0819 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0677 ,  Y10T137/0318 ,  Y10T436/12 ,  Y10T436/2575

Abstract: A system and method for reducing the number of input/output connections required to connect a microfluidic substrate to an external controller for controlling the substrate. In one example, a microfluidic processing device is fabricated on a substrate having a plurality of N independently controllable components, (e.g., a resistive heating elements) each having at least two terminals. The substrate includes a plurality of input/output contacts for connecting the substrate to an external controller, and a plurality of leads for connecting the contacts to the terminals of the components. The leads are arranged to allow the external controller to supply control signals to the terminals of the components via the contacts using substantially fewer contacts than the total number of component terminals. For example, in one embodiment, each lead connects a corresponding contact to a plurality of terminals to allow the controller to supply to signals to the terminals without requiring a separate contact for each terminal. However, to assure that the components can each be controlled independently of the others, the leads are also arranged so that each component's terminals are connected to a unique combination of contacts. Thus, the external controller can activate a selected component by supplying control signals to the combination of contacts uniquely associated with that component.

Abstract translation: 一种用于减少将微流体衬底连接到用于控制衬底的外部控制器所需的输入/输出连接的数量的系统和方法。 在一个实例中，在具有多个N个可独立控制的部件（例如，电阻加热元件）的基板上制造微流体处理装置，每个具有至少两个端子。 基板包括用于将基板连接到外部控制器的多个输入/输出触点，以及用于将触点连接到部件的端子的多个引线。 引线被布置成允许外部控制器通过使用比组件端子总数少得多的触点经由触点向组件的端子提供控制信号。 例如，在一个实施例中，每个引线将对应的触点连接到多个端子，以允许控制器向终端提供信号，而不需要为每个终端单独的接触。 然而，为了确保各个部件可以独立于其他部件来控制，引线也被布置成使得每个部件的端子连接到独特的触头组合。 因此，外部控制器可以通过向与该组件唯一相关联的联系人的组合提供控制信号来激活所选择的组件。

公开(公告)号：WO03012406A9

公开(公告)日：2003-03-20

申请号：PCT/US0209440

申请日：2002-03-27

Applicant: HANDYLAB INC

Inventor： PARUNAK GENE ,  HANDIQUE KALYAN ,  WU BETTY ,  GANESAN KARTHIK

IPC: G11C16/06 ,  B01L3/00 ,  B81B1/00 ,  F04B19/00 ,  F04B19/24 ,  F04F1/06 ,  G01N21/29 ,  B01D57/02 ,  C02F1/40 ,  C12M1/34 ,  C12M1/36 ,  C12N13/00 ,  C12Q1/68

CPC classification number: B01L3/502738 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2400/0442

Abstract: The present invention relates to a system and method for moving samples, such as fluids, within a microfluidic system using a plurality of gas actuators (168, 170, 172) for applying pressure at different locations within the microfluidic. The system includes a substrate (130, 132) which forms a fluid network through which fluid flows, and a plurality of gas actuators (168, 170, 172) integral with the substrate (130, 132). One such actuator (168) is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator (170) is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve (915, 216) is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.

Abstract translation: 本发明涉及一种使用多个用于在微流体内的不同位置施加压力的气体致动器（168,170,172）在微流体系统内移动样品（例如流体）的系统和方法。 该系统包括形成流体流过的流体网络的衬底（130,132）和与衬底（130,132）成一体的多个气体致动器（168,170,172）。 一个这样的致动器（168）在第一位置处联接到网络，以提供气体压力以移动网络内的微流体样本。 另一气体致动器（170）在第二位置处联接到网络，用于提供气体压力以进一步移动网络内微流体样品的至少一部分。 阀（915,216）联接到微流体网络，使得当阀关闭时，阀将基本上将第二气体致动器与第一气体致动器隔离。

公开(公告)号：WO2007112114A2

公开(公告)日：2007-10-04

申请号：PCT/US2007007513

申请日：2007-03-26

Applicant: HANDYLAB INC ,  HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

Inventor： HANDIQUE KALYAN ,  BRAHMASANDRA SUNDARESH N ,  GANESAN KARTHIK ,  WU BETTY ,  PHADKE NIKHIL ,  PARUNAK GENE ,  WILLIAMS JEFF

IPC: C12M3/00

CPC classification number: B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/06 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/18 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0442 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0611 ,  B01L2400/0677 ,  B01L2400/0683 ,  F16K99/0001 ,  F16K99/003 ,  F16K99/0032 ,  F16K99/0044 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N2035/00881

Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Abstract translation: 该专利申请描述了用于处理含有多核苷酸的样品并用于在其上提供诊断结果的集成设备。 该装置被配置为接收包含用于处理样品的试剂和网络的微流控盒。 还描述了使用该装置的方法。

公开(公告)号：WO2005011867A3

公开(公告)日：2005-04-21

申请号：PCT/US2004025181

申请日：2004-08-02

Applicant: HANDYLAB INC ,  WU BETTY ,  HANDIQUE KALYAN ,  PARUNAK GENE ,  KEHRER AARON ,  GANESAN KARTHIK

Inventor： WU BETTY ,  HANDIQUE KALYAN ,  PARUNAK GENE ,  KEHRER AARON ,  GANESAN KARTHIK

IPC: B01L3/00 ,  B01L99/00 ,  B81B1/00

CPC classification number: C12Q1/6806 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0677 ,  B33Y40/00 ,  B33Y80/00 ,  C12Q2523/109 ,  G01N1/40 ,  Y10T436/25 ,  Y10T436/25375

Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Abstract translation: 微流体装置包括用于将含颗粒液体样品输入装置的输入口，保持构件和压力致动器。 保持构件与输入端口连通并且构造成将含颗粒液体样本的颗粒与含有流体样本的颗粒的液体的第一部分在空间上分离。 压力致动器将至少一些分离的颗粒与从颗粒分离的液体的第一部分的子集重新结合。 该装置还可以包括从保留部件接收颗粒和液体的裂解室。 裂解室热溶解颗粒以释放其内容物。