公开(公告)号：WO2002068686A1

公开(公告)日：2002-09-06

申请号：PCT/GB2002/000868

申请日：2002-02-27

Applicant: ISIS INNOVATION LIMITED ,  COOK, Peter, R. ,  KIMURA, Hiroshi

Inventor： COOK, Peter, R. ,  KIMURA, Hiroshi

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q2539/103 ,  C12Q2525/113 ,  C12Q2521/313 ,  C12Q2563/179

Abstract: The invention relates to the detection and analysis of RNA transcripts, and in particular to methods of characterising the numbers and types of primary RNA transcripts produced in a given cell or tissue.

Abstract translation: 本发明涉及RNA转录物的检测和分析，特别涉及表征给定细胞或组织中产生的初级RNA转录物的数目和类型的方法。

公开(公告)号：WO2002061129A2

公开(公告)日：2002-08-08

申请号：PCT/US2001/045845

申请日：2001-11-15

Applicant: MINERVA BIOTECHNOLOGIES CORPORATION

Inventor： BAMDAD, Cynthia, Carol ,  BAMBAD, R., Shoshana

IPC: C12Q1/68

CPC classification number: C40B30/04 ,  B82Y30/00 ,  C12N15/1055 ,  C12Q1/6804 ,  C12Q1/6834 ,  G01N33/532 ,  G01N33/58 ,  G01N33/6842 ,  G01N33/6845 ,  C12Q2565/201 ,  C12Q2563/179 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q2563/137

Abstract: Methods, assays, and components are described in which biological samples can be rapidly and sensitively analyzed for the presence of species associated with neurodegenerative disease. Techniques and components are provided for diagnosis of disease, as well as for screening of candidate drugs for treatment of neurodegenerative disease. The techniques are simple, extremely sensitive, and utilize readily-available components. Binding species, capable of binding a neurodegenerative disease aggregate-forming or aggregate-forming species, are fastened to surfaces of electrodes and surfaces of particles, or provided free in solution, to bind aggregate-forming species and/or be involved in aggregation.

Abstract translation: 描述了方法，测定和组分，其中可以快速和敏感地分析生物样品中与神经变性疾病相关的物种的存在。 提供技术和组件用于诊断疾病，以及筛选用于治疗神经变性疾病的候选药物。 这些技术简单，极其敏感，并且利用易于使用的组件。 能够结合神经变性疾病聚集体形成或聚集形成物质的结合物质被紧固到电极和颗粒表面的表面，或者在溶液中自由形成，从而结合聚集体形成物质和/或参与聚集。

公开(公告)号：WO0227029A2

公开(公告)日：2002-04-04

申请号：PCT/US0130396

申请日：2001-09-27

Applicant: LYNX THERAPEUTICS INC

Inventor： FU RONGDIAN ,  BRENNER SYDNEY ,  ALBRECHT GLENN

IPC: C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q2545/114 ,  C12Q2563/179 ,  C12Q2565/626

Abstract: Disclosed are methods for identifying nucleic acid sequences which are of different abundances in different nucleic acid source populations, e.g. differentially expressed genes or genomic variations among individuals or populations of individuals. In one embodiment, probes derived from the source nucleic acid populations are derivatized with a terminal sample ID (SID) sequence characteristic of that population. Upon competitive hybridization of the probes to a reference or index nucleic acid library containing all the sequences in the populations being compared, the SID tags remain single stranded, and those from the different sources are then annealed to one another. Unhybridized (remainder) SID sequences are then quantified. By labeling such remainder SID sequences with a fluorescent dye, FACS sorting of beads containing the hybridized probes can be carried out. The signal ratio upon which such sorting is based is enhanced compared to competitive hybridization using labeled probes without SID sequences.

Abstract translation: 公开了用于鉴定在不同核酸源群体中具有不同丰度的核酸序列的方法，例如， 差异表达基因或个体个体或个体群体的基因组变异。 在一个实施方案中，衍生自源核酸群体的探针用该群体特征的终端样品ID（SID）序列衍生化。 当探针与包含正在比较的群体中的所有序列的参考或指数核酸文库竞争性杂交时，SID标签保持单链，然后来自不同来源的那些序列彼此退火。 然后对未杂交（其余）SID序列进行定量。 通过用荧光染料标记这些剩余的SID序列，可以进行含有杂交探针的珠的FACS分选。 与使用没有SID序列的标记探针的竞争性杂交相比，这种分选所基于的信号比率增加。

公开(公告)号：WO0056937A3

公开(公告)日：2002-02-07

申请号：PCT/US0008070

申请日：2000-03-27

Applicant: HYSEQ INC

Inventor： DRMANAC RADOJE

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566

CPC classification number: C12Q1/6869 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q2565/631 ,  C12Q2565/628 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2565/518

Abstract: Novel solution-based methods and materials, including apparatus, for sequence analysis by hybridization are provided.

Abstract translation: 提供了基于溶液的方法和材料，包括通过杂交进行序列分析的装置。

公开(公告)号：WO01021840A3

公开(公告)日：2001-12-06

申请号：PCT/US2000/026207

申请日：2000-09-25

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12Q2563/179 ,  C12Q2535/138 ,  C12Q2525/131

Abstract: The invention relates to method and devices for indexing populations, among other things. Particular embodiments relate to methods for indexing population that can be carried out in homogeneous media, particularly methods in which indexing reactions can be carried out in homogeneous media and the products of the reactions can be determined without physically separating the products from the reaction. In these and other regards the invention further relates to methods and devices for indexing using real time monitoring and determinations based on reaction kinetics. In another further respect in these and other regards the invention relates to multiplexed indexing. And, in a still further specific in all these regards the invention relates to methods and devices for indexing a substantial fraction of all sub-populations of a given type. Specific embodiments relate to polynucleotides to expression profiling and to strand displacement indexing and reagent for strand displacement indexing.

Abstract translation: 本发明涉及用于索引人群的方法和装置等等。 特别的实施方案涉及可以在均相介质中进行的分子标记的方法，特别是可以在均相介质中进行分选反应的方法，并且可以确定反应产物而不将产物与反应物理分离。 在这些和其他方面，本发明还涉及使用基于反应动力学的实时监测和测定来进行索引的方法和装置。 在这些和其他方面的另一方面，本发明涉及多重索引。 并且，在所有这些方面的更进一步的具体内容中，本发明涉及用于索引给定类型的所有子种群的大部分的方法和装置。 具体实施方案涉及用于表达谱的多核苷酸和用于链置换分度的链置换分度和试剂。

公开(公告)号：WO0000638A3

公开(公告)日：2000-04-20

申请号：PCT/EP9904290

申请日：1999-06-21

Applicant: AKZO NOBEL NV ,  SILLEKENS PETER THEODORUS GERA ,  DEURSEN PETRUS BERNARDUS HUGO ,  KOK WESSEL

Inventor： SILLEKENS PETER THEODORUS GERA ,  VAN DEURSEN PETRUS BERNARDUS H ,  KOK WESSEL

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q2563/179 ,  C12Q2525/161

Abstract: The present invention concerns a method for tagging the reaction products of transcription-based amplification procedures, and a kit for detecting such tagged nucleic acid amplified in a transcription-based amplification assay. The method is characterised in that RNA amplicons are generated by transcription-based amplification by introducing transcribable non-target related sequence elements in one or both primers used for transcription-based amplification which are transcribed into the RNA amplicons. In particular, the extra sequence elements thus introduced into the amplicons, can be used for the detection of the amplicons, for specific selection of the amplicons, to artificially extend the length of amplicons or to tag polypeptides translated from RNA amplicons generated by transcription-based amplification.

Abstract translation: 本发明涉及用于标记基于转录的扩增程序的反应产物的方法，以及用于检测在基于转录的扩增测定中扩增的标记的核酸的试剂盒。 该方法的特征在于通过在用于基于转录的扩增的一种或两种引物中引入可转录的非靶标相关序列元件，通过基于转录的扩增生成RNA扩增子，所述扩增子转录成RNA扩增子。 具体而言，如此引入扩增子的额外序列元件可用于检测扩增子，用于扩增子的特异性选择，人工延长扩增子的长度或标记由基于转录的RNA扩增子翻译的多肽 放大。

公开(公告)号：WO00020639A1

公开(公告)日：2000-04-13

申请号：PCT/US1999/022585

申请日：1999-09-28

IPC: C07B61/00 ,  C07H21/00 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC classification number: C07H21/00 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/6816 ,  C40B40/00 ,  C12Q2563/179 ,  C12Q2533/107 ,  C12Q2521/313

Abstract: The invention provides oligonucleotide tag compositions and methods for synthesizing repertoires of error-free oligonucleotide tags that may be used for labeling and sorting polynucleotides, such as cDNAs, restriction fragments, and the like. In accordance with the method of the invention, oligonucleotide tag precursors are provided in an amplicon, wherein the tag precursors each consists of one or more oligonucleotide "words" selected from the same minimally cross-hybridizing set of words. The oligonucleotide tag precursors are elongated by repeated cycles of cleavage, ligation of one or more words, and amplification. Cycles continue until the oligonucleotide tags of the repertoire have a desired length or complexity.

Abstract translation: 本发明提供寡核苷酸标签组合物和用于合成可用于标记和分选多核苷酸如cDNA，限制片段等的无错误寡核苷酸标签的所有组成成分的方法。 根据本发明的方法，在扩增子中提供寡核苷酸标签前体，其中标签前体各自由一个或多个选自相同的最小交叉杂交的词组的寡核苷酸“词”组成。 寡核苷酸标签前体通过重复的切割循环，一个或多个单词的连接和扩增而延长。 循环继续，直到谱体的寡核苷酸标签具有期望的长度或复杂度。

公开(公告)号：WO00000638A2

公开(公告)日：2000-01-06

申请号：PCT/EP1999/004290

申请日：1999-06-21

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q2563/179 ,  C12Q2525/161

Abstract: The present invention concerns a method for tagging the reaction products of transcription-based amplification procedures, and a kit for detecting such tagged nucleic acid amplified in a transcription-based amplification assay. The method is characterised in that RNA amplicons are generated by transcription-based amplification by introducing transcribable non-target related sequence elements in one or both primers used for transcription-based amplification which are transcribed into the RNA amplicons. In particular, the extra sequence elements thus introduced into the amplicons, can be used for the detection of the amplicons, for specific selection of the amplicons, to artificially extend the length of amplicons or to tag polypeptides translated from RNA amplicons generated by transcription-based amplification.

Abstract translation: 本发明涉及用于标记基于转录的扩增方法的反应产物的方法，以及用于检测在基于转录的扩增测定中扩增的标记核酸的试剂盒。 该方法的特征在于RNA扩增子通过基于转录的扩增产生，其通过在转录到RNA扩增子中的用于基于转录的扩增的一个或两个引物中引入可转录的非靶相关序列元件。 特别地，由此引入到扩增子中的额外的序列元件可用于扩增子的检测，扩增子的特异选择，人工延长扩增子的长度或标记从转录生成的RNA扩增子翻译的多肽 放大。

公开(公告)号：WO98055657A1

公开(公告)日：1998-12-10

申请号：PCT/US1998/011825

申请日：1998-06-05

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1051 ,  C12Q1/6837 ,  C12Q2563/179

Abstract: The current invention provides an accurate and reliable method for tagging gene sequences for future identification. The method uses a specific identification serial number that may be one or more characters, with each character being encoded by a distinct sequence of nucleic acids. These nucleic acids are referred to as the serial number nucleic acids. The distinct sequence of nucleic acids is attached to a given genetic sequence so that the genetic sequence will always be identifiable by one reading the serial number nucleic acids.

Abstract translation: 本发明提供了用于标记基因序列以用于将来鉴定的准确可靠的方法。 该方法使用可以是一个或多个字符的特定识别序列号，每个字符由不同的核酸序列编码。 这些核酸被称为序列号核酸。 核酸的不同序列连接到给定的遗传序列，使得遗传序列总是可以通过读取序列号核酸来鉴定。

公开(公告)号：WO1997019193A2

公开(公告)日：1997-05-29

申请号：PCT/US1996018812

申请日：1996-11-21

Applicant: YALE UNIVERSITY

Inventor： YALE UNIVERSITY ,  LIZARDI, Paul M. ,  CAPLAN, Michael

IPC: C12Q01/68

CPC classification number: C12Q1/6862 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2563/179 ,  C12Q2531/125 ,  C12Q2531/143 ,  C12Q2537/125 ,  C12Q2531/119 ,  C12Q2537/143 ,  C12Q2539/113 ,  C12Q2565/102 ,  C12Q2525/161 ,  C12Q2563/131 ,  C12Q2525/307

Abstract: Disclosed are compositions and a method for amplification of and multiplex detection of molecules of interest involving rolling circle replication. The method is useful for simultaneously detecting multiple specific nucleic acids in a sample with high specificity and sensitivity. The method also has an inherently low level of background signal. A preferred form of the method consists of an association operation, an amplification operation, and a detection operation. The association operation involves association of one ore more specially designed probe molecules, either wholly or partly nucleic acid, to target molecules of interest. This operation associates the probe molecules to a target molecule present in a sample. The amplification operation is rolling circle replication of circular nucleic acid molecules, termed amplification target circles, that are either a part of, or hybridized to, the probe molecules. A single round of amplification using rolling circle replication results in a large amplification of the amplification target circles. Following rolling circle replication, the amplified sequences are detected using combinatorial multicolor coding probes that allow separate, simultaneous, and quantitative detection of multiple different amplified target circles representing multiple different target molecules. Since the amplified product is directly proportional to the amount of target sequence present in a sample, quantitative measurements reliably represent the amount of a target sequence in a sample. Major advantages of this method are that a large number of distinct target molecules can be detected simultaneously, and that differences in the amounts of the various target molecules in a sample can be accurately quantified. It is also advantageous that the DNA replication step is isothermal, and that signals are strictly quantitative because the amplification reaction is linear and is catalyzed by a highly processive enzyme.

Abstract translation: 公开了用于扩增和多重检测涉及滚环复制的目标分子的组合物和方法。 该方法对于以高特异性和灵敏度同时检测样品中的多种特异性核酸是有用的。 该方法也具有固有的低水平的背景信号。 该方法的优选形式包括关联操作，放大操作和检测操作。 关联操作涉及将一个或多个特别设计的探针分子（全部或部分核酸）与目的目的分子结合。 该操作将探针分子与存在于样品中的靶分子相关联。 扩增操作是圆环核酸分子的圆圈复制，称为扩增靶圆，它们是探针分子的一部分或杂交的。 使用滚圆复制的单次放大导致放大目标圆的大放大。 在循环复制之后，使用组合多色编码探针检测扩增的序列，其允许分离，同时和定量检测表示多个不同靶分子的多个不同扩增的目标圆。 由于扩增产物与样本中存在的靶序列的量成正比，所以定量测量可靠地表示样品中靶序列的量。 该方法的主要优点是可以同时检测大量不同的目标分子，并且可以精确量化样品中各种靶分子的量的差异。 还有利的是，DNA复制步骤是等温的，并且信号是严格定量的，因为扩增反应是线性的，并被高度进行的酶催化。