IMPROVED PROCESS FOR FRACTIONATION OF LIGNOCELLULOSIC BIOMASS
    184.
    发明申请
    IMPROVED PROCESS FOR FRACTIONATION OF LIGNOCELLULOSIC BIOMASS 审中-公开
    木质纤维素生物质分级的改进方法

    公开(公告)号:WO2011097075A2

    公开(公告)日:2011-08-11

    申请号:PCT/US2011/022000

    申请日:2011-01-21

    Abstract: Methods are provided for the efficient fractionation of lignocellulosic biomasses into cellulosic, hemicellulosic and lignin fractions, wherein concentrated organic acid vapors are applied to the biomass at elevated temperatures at the location(s) or near the location(s) where the biomass has been harvested and gathered, to at least partly depolymerize or substantially solubilize the hemicelluloses and lignins in the biomass. The organic acid-treated biomass is in either case then dried and pelletized for extended bulk storage and/or for shipment to a second facility some distance away. The organic acid-treated biomass may be processed into desired chemicals, fuels and/or fuel additives at the local processing site or at a second facility away from the local processing site, or the pelletized material may be used as a ruminant feed locally or at a feedlot some distance removed from the local processing site.

    Abstract translation: 提供了将木质纤维素生物质有效分馏成纤维素,半纤维素和木质素部分的方法,其中将浓缩的有机酸蒸气在升高的温度下施用于生物质的位置处或附近的位置( s),其中收获和收集生物质,以至少部分解聚或基本上溶解生物质中的半纤维素和木质素。 经过有机酸处理的生物质在任一情况下都经过干燥和造粒以延长大容量储存和/或运送到距离一定距离的第二设施。 有机酸处理过的生物质可以在当地处理场所或远离当地处理场所的第二设施处理成期望的化学品,燃料和/或燃料添加剂,或者该粒状材料可以局部用作反刍动物饲料或 一个距离本地处理站点一定距离的饲养场。

    PEPTIDE DOMAINS THAT BIND SMALL MOLECULES OF INDUSTRIAL SIGNIFICANCE
    185.
    发明申请
    PEPTIDE DOMAINS THAT BIND SMALL MOLECULES OF INDUSTRIAL SIGNIFICANCE 审中-公开
    肽结构域与小分子的工业意义相结合

    公开(公告)号:WO2011094617A8

    公开(公告)日:2011-08-04

    申请号:PCT/US2011/023030

    申请日:2011-01-28

    Abstract: Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

    Abstract translation: 本文描述了小肽结构域和共有序列,其结合具有工业重要性的小目标分子,例如金属如镍,β胡萝卜素和异黄酮如染料木黄酮。 还描述了融合蛋白质,其含有与蛋白质融合的这种结合结构域或与GST或CBD结合的肽结构域,其与其他配体结合并可用于将靶结合域固定在载体上。 在工业环境中有用的一类融合蛋白是含有靶结合结构域的多联体的融合体,其增加每个分子的结合等价物。

    ACIDIC GAS PERMEATED CARBOXYALKYL STARCH PARTICLES, EXTRUDATES, AND PROCESS FOR MAKING THE SAME
    188.
    发明申请
    ACIDIC GAS PERMEATED CARBOXYALKYL STARCH PARTICLES, EXTRUDATES, AND PROCESS FOR MAKING THE SAME 审中-公开
    酸性气体渗透性羧甲基淀粉颗粒,其制备方法及其制备方法

    公开(公告)号:WO2010096742A2

    公开(公告)日:2010-08-26

    申请号:PCT/US2010/024872

    申请日:2010-02-22

    Abstract: The present disclosure relates to particles comprising carboxyalkyl starch that are permeated with an acidic gas and their uses as absorbent materials. It was discovered that superabsorbent materials could be obtained from carboxyalkyl starch particles permeated with the acidic gas and heated to a temperature of at least 100°C until they reach an AUL at 0.7 psi. of at least 14 g/g and a CRC of at least 18 g/g. Moreover, it was discovered that the pH of alkaline starch extrudates can be adjusted by permeating particles of the extrudate with the acidic gas even with treating the particles to temperatures less than 100°C. The carboxyalkyl starch particles obtained by the methods described herein are characterized as having intramolecular ester bonds, which are greater in number at the surface of the particle than in the core, and the particles have a greater concentration of cation of the acidic gas at the surface than a the core.

    Abstract translation: 本公开涉及包含羧酸烷基淀粉的颗粒,其被酸性气体渗透并且其用作吸收材料。 发现超吸收材料可以从用酸性气体渗透的羧基烷基淀粉颗粒获得并加热到至少100℃的温度,直到它们在0.7psi下达到AUL。 至少为14g / g,CRC为至少18g / g。 此外,发现即使将颗粒处理到低于100℃的温度,也可以通过用酸性气体渗透挤出物的颗粒来调节碱性淀粉挤出物的pH。 通过本文描述的方法获得的羧基烷基淀粉颗粒的特征在于具有分子内酯键,其在颗粒表面上的数量大于芯中的数量,并且颗粒在表面具有较高浓度的酸性气体的阳离子 比核心。

    DEVELOPMENT OF STRAINS OF THE THERMOTOLERANT YEAST HANSENULA POLYMORPHA CAPABLE OF ALCOHOLIC FERMENTATION OF STARCH AND XYLAN BY EXPRESSION OF STARCH AND XYLAN DEGRADING ENZYMES
    190.
    发明申请
    DEVELOPMENT OF STRAINS OF THE THERMOTOLERANT YEAST HANSENULA POLYMORPHA CAPABLE OF ALCOHOLIC FERMENTATION OF STARCH AND XYLAN BY EXPRESSION OF STARCH AND XYLAN DEGRADING ENZYMES 审中-公开
    通过淀粉和XYLAN降解酶的表达可以发现热稳定性YEAST HANSENULA多糖的菌株能够发酵淀粉和XYLAN的醇溶性发酵

    公开(公告)号:WO2009137574A2

    公开(公告)日:2009-11-12

    申请号:PCT/US2009/042975

    申请日:2009-05-06

    Abstract: Genes SWA2 and GAMl from the yeast, Schwanniomyces occidentalis , encoding α- amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha . The expression was achieved by integration of the SWA2 and GAM l genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha - glyceraldehyde-3 -phosphate dehydrogenase gene ( HpGAP . Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee , and a xlnD gene coding for β-xylosidase was obtained from the fungus Aspergillus niger . Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.

    Abstract translation: 来自酵母的基因SWA2和GAM1分别编码α-淀粉酶和葡糖淀粉酶,分别在多形汉逊酵母中表达。 通过与多形汉逊酵母 - 甘油醛-3-磷酸脱氢酶基因(HpGAP)的强组成型启动子可操作地连接,将SWA2和GAM1基因整合到多形汉逊酵母的染色体中来实现该表达。所得到的转化体获得了生长能力 在含有可溶性淀粉作为唯一碳源的基本培养基上,可以从淀粉高温发酵至10g / L生产乙醇,从真菌木霉菌获得编码内切木聚糖酶的XYN2基因,编码ß的xlnD基因 这些基因的共表达也通过在HpGAP启动子控制下融合到多形汉逊酵母染色体中而得到,所得转化体能够在补充有桦木木聚糖的基本培养基上生长 单独的碳源成功表达木聚糖酶导致能够发酵桦木木聚糖的受体菌株 到48℃的乙醇。此外,在多克隆多因子菌株中共表达过去基因,其过表达丙酮酸脱羧酶基因进一步改善乙醇生产。

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