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    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION
    14.
    发明申请
    TYPE II FATTY ACID SYSTHESIS ENZYMES IN REVERSE B-OXIDATION 审中-公开
    II型脂肪酸在反向氧化中的合成酶

    公开(公告)号：WO2015112988A1

    公开(公告)日：2015-07-30

    申请号：PCT/US2015/012932

    申请日：2015-01-26

    Applicant: WILLIAM MARSH RICE UNIVERSITY

    Inventor： GONZALEZ, Ramon ,  CLOMBURG, James, M. ,  VICK, Jacob, E.

    IPC: C10L1/06

    CPC classification number: C12P7/64 ,  C10L1/02 ,  C10L1/04 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/03013 ,  C12Y102/01 ,  C12Y102/01003 ,  C12Y103/01009 ,  C12Y114/00 ,  C12Y203/01009 ,  C12Y206/00 ,  C12Y401/99005 ,  C12Y402/01059 ,  Y02E50/343

    Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis ("FAS") pathway that can be used in combination with thiolases to operate a functional reversal of the β-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the β-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their α-, β-, and ω-functionalized derivatives from renewable carbon sources can be achieved.

    Abstract translation: 本公开描述了可以与硫醇酶组合使用以操作β-氧化循环的功能逆转的来自II型（一组离散的酶）脂肪酸合成（“FAS”）途径的酶。 3-硫基酶与一个或多个3-氧基酰基 - [酰基 - 载体 - 蛋白]还原酶（FabG，其它），3-羟基酰基 - [acp]脱水酶（FabA，FabZ等）和烯酰基 - - 蛋白]还原酶（FabI，FabK，FabL，FabV等）产生β-氧化循环的功能逆转。 如果只使用一种或两种酶，剩余的酶将是传统的β氧化酶。 一旦该循环与适当的引发和终止途径相结合，就可以实现羧酸，醇，烃，胺及其可再生碳源的α-，β-和ω-官能化衍生物的生产。

    MUTANT AOX 1 PROMOTERS
    15.
    发明申请
    MUTANT AOX 1 PROMOTERS 审中-公开

    公开(公告)号：WO2006089329A3

    公开(公告)日：2007-05-31

    申请号：PCT/AT2006000079

    申请日：2006-02-23

    Applicant: UNIV GRAZ TECH ,  HARTNER FRANZ ,  GLIEDER ANTON

    Inventor： HARTNER FRANZ ,  GLIEDER ANTON

    IPC: C12N15/00

    CPC classification number: C12N15/815 ,  C12Y101/03013

    Abstract: A mutant Pichia pastoris alcohol oxidase 1 (AOXl) promoter of the wild type Pichia pastoris AOXl promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of : a) a transcription factor binding site (TFBS) , b) nucleotides 170 to 235 (-784 to -719) , nucleotides 170 to 191 (-784 to -763) , nucleotides 192 to 213 (-762 to -741) , nucleotides 192 to 210 (-762 to -744) , nucleotides 207 to 209 (-747 to -745) , nucleotides 214 to 235 (-740 to -719) , nucleotides 304 to 350 (-650 to -604) , nucleotides 364 to 393 (-590 to -561) , nucleotides 434 to 508 (-520 to -446) , nucleotides 509 to 551 (-445 to -403) , nucleotides 552 to 560 (-402 to -394) , nucleotides 585 to 617 (-369 to -337) , nucleotides 621 to 660 (-333 to -294) , nucleotides 625 to 683 (-329 to -271) , nucleotides 736 to 741 (-218 to -213) , nucleotides 737 to 738 (-217 to -216) , nucleotides 726 to 755 (-228 to -199) , nucleotides 784 to 800 (-170 to -154) or nucleotides 823 to 861 (-131 to -93) of Seq ID No. 1, and combinations thereof .

    Abstract translation: 包含选自以下的至少一个突变的野生型巴斯德毕赤酵母AOX1启动子（SEQ ID No.1）的突变型巴斯德毕赤酵母醇氧化酶1（AOX1）启动子：a）转录因子结合位点（TFBS），b ）核苷酸170至235（-784至-719），核苷酸170至191（-784至-763），核苷酸192至213（-762至-741），核苷酸192至210（-762至-744），核苷酸 207至209（-747至-745），核苷酸214至235（-740至-719），核苷酸304至350（-650至-604），核苷酸364至393（-590至-561），核苷酸434至 508（-520至-446），核苷酸509至551（-445至-403），核苷酸552至560（-402至-394），核苷酸585至617（-369至-337），核苷酸621至660（ -333至-294），核苷酸625至683（-329至-271），核苷酸736至741（-218至-213），核苷酸737至738（-217至-216），核苷酸726至755（-228 至-199），核苷酸784至800（-170至-154）或核苷酸823至861（-131至-9 3）Seq ID No.1及其组合。

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