公开(公告)号：WO2013082307A1

公开(公告)日：2013-06-06

申请号：PCT/US2012/067110

申请日：2012-11-29

Applicant: C2N DIAGNOSTICS

Inventor： WEST, Tim ,  PAOLETTI, Andrew, Corey

IPC: G01N33/483

CPC classification number: G01N33/6896 ,  G01N33/5308 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2458/15 ,  G01N2560/00 ,  G01N2800/28 ,  G06F19/20

Abstract: The present invention provides methods for measuring the absolute concentration of a biomolecule of interest in a subject. Such biomolecules may be implicated in one or more neurological and neurodegenerative diseases or disorders. Also provided is a method for determining whether a therapeutic agent affects the in vivo metabolism of a central nervous system derived biomolecule. Also provided are kits for performing the methods of the invention.

Abstract translation: 本发明提供了测量受试者感兴趣的生物分子的绝对浓度的方法。 这样的生物分子可能涉及一种或多种神经和神经变性疾病或病症。 还提供了一种用于确定治疗剂是否影响衍生的中枢神经系统生物分子的体内代谢的方法。 还提供了用于实施本发明的方法的试剂盒。

公开(公告)号：WO2013073929A1

公开(公告)日：2013-05-23

申请号：PCT/MY2012/000273

申请日：2012-11-14

Applicant: ACGT INTELLECTUAL LIMITED ,  ACGT, Sdn Bhd

Inventor： POH, Yang, Ming ,  BOON, Soo, Heong ,  LEE, Ying, Wah

IPC: C12Q1/68 ,  G06F19/10

CPC classification number: C12Q1/6827 ,  G06F19/20 ,  G06F19/22 ,  C12Q2537/165 ,  C12Q2565/501

Abstract: The invention relates to a method for detecting at least one nucleic acid variation based on the ratio of corrected signal intensities of at least two differentially labelled probes capable of detecting the nucleic acid variation. The invention also relates to an apparatus for performing the method.

Abstract translation: 本发明涉及一种用于基于能够检测核酸变异的至少两个差异标记的探针的校正信号强度的比率来检测至少一种核酸变异的方法。 本发明还涉及一种用于执行该方法的装置。

公开(公告)号：WO2013040697A1

公开(公告)日：2013-03-28

申请号：PCT/CA2012/000893

申请日：2012-09-21

Applicant: TROST, Brett ,  KUSALIK, Anthony ,  NAPPER, Scott ,  ARSENAULT, Ryan ,  GRIEBEL, Philip

Inventor： TROST, Brett ,  KUSALIK, Anthony ,  NAPPER, Scott ,  ARSENAULT, Ryan ,  GRIEBEL, Philip

IPC: C40B40/10 ,  C40B30/00 ,  C40B30/02 ,  C40B50/02 ,  G01N33/50 ,  G01N33/68 ,  G06F19/28

CPC classification number: G06F19/20 ,  C07K1/047 ,  G01N2440/14 ,  G06F19/22 ,  G06F19/24 ,  G06F19/28

Abstract: A method of preparing a species-specific phosphorylation site peptide array for a target organism comprising: a) selecting a plurality of known non-target organism (NTO) phosphorylation site sequences and cognate known NTO phosphorylation polypeptide sequences from one or more NTO, each of the known NTO phosphorylation site sequences comprising at least 5 residues and less than 30 residues; b) identifying a matching target organism (TO) phosphorylation site sequence and cognate TO phosphorylation polypeptide sequence for one or more of the known NTO phosphorylation site sequences; c) determining the matching TO phosphorylation site sequences that correspond to orthologue polypeptides of the cognate known NTO phosphorylation polypeptide sequences; d) selecting the matching TO phosphorylation site sequences determined to correspond to orthologue polypeptides for inclusion on the array; wherein the matching TO phosphorylation site sequences that correspond to orthologue polypeptides are determined by calculating, for each matching phosphorylation site sequence identified in b), a similarity value between the TO phosphorylation polypeptide sequence corresponding to the TO phosphorylation site sequence and a TO polypeptide sequence matching the cognate known NTO polypeptide sequence.

Abstract translation: 制备靶生物的物种特异性磷酸化位点肽阵列的方法，包括：a）从一个或多个NTO中选择多个已知的非靶生物（NTO）磷酸化位点序列和同源的已知NTO磷酸化多肽序列，每个 已知的NTO磷酸化位点序列包含至少5个残基和少于30个残基; b）鉴定一种或多种已知NTO磷酸化位点序列的匹配靶生物（TO）磷酸化位点序列和同源TO磷酸化多肽序列; c）确定与同源已知的NTO磷酸化多肽序列的直向同源多肽相对应的匹配的TO磷酸化位点序列; d）选择确定为对应于直向同源多肽的匹配的TO磷酸化位点序列以包含在阵列上; 其中对应于直向同源多肽的匹配的TO磷酸化位点序列通过对于b）中鉴定的每个匹配的磷酸化位点序列计算，对应于TO磷酸化位点序列的TO磷酸化多肽序列与匹配的TO多肽序列之间的相似性值 同源已知的NTO多肽序列。

公开(公告)号：WO2013039467A1

公开(公告)日：2013-03-21

申请号：PCT/US2011/051147

申请日：2011-09-12

Applicant: KING SAUD UNIVERSITY ,  HART, Brian G. ,  KHAN, Haseeb Ahmad ,  ARIF, Ibrahim Abdulwahid

Inventor： HART, Brian G. ,  KHAN, Haseeb Ahmad ,  ARIF, Ibrahim Abdulwahid

IPC: G06F19/10

CPC classification number: G06F19/20 ,  G06F19/24

Abstract: Indexing gene expression data for comparing gene signatures includes assigning one of a plurality of fold change-based grading scores to each of a number of genes in a probe gene signature. The fold change-based grading scores reflect relative expression of one of the number of genes in the probe gene signature. Each of the number of genes in the probe gene signature assigned a particular grading score is weighted by the particular grading score. A ratio of each weighted number of genes in the probe gene signature assigned a particular grading score to a total number of genes in the probe gene signature is determined. Then, ratios of each weighted number of genes in the probe gene signature assigned each particular grading score to the total number of genes in the probe gene signature are summed to generate an index of gene expression.

Abstract translation: 索引用于比较基因特征的基因表达数据包括将多个基于倍数变化的分级分数中的一个分配给探针基因签名中的多个基因中的每一个。 基于倍数变化的分级得分反映了探针基因签名中基因数目之一的相对表达。 分配给特定分级评分的探针基因签名中的每个基因的数量由特定的分级分数加权。 确定分配特定分级评分的探针基因签名中每个加权数目与探针基因签名中基因总数的比例。 然后，将分配给每个特定分级评分的探针基因签名中的每个加权数量与探针基因签名中的基因总数的比率相加以产生基因表达指数。

公开(公告)号：WO2013011479A3

公开(公告)日：2013-03-14

申请号：PCT/IB2012053686

申请日：2012-07-19

Applicant: KONINKL PHILIPS ELECTRONICS NV ,  VERHAEGH WILHELMUS FRANCISCUS JOHANNES ,  VAN DE STOLPE ANJA ,  VAN OOIJEN HENDRIK JAN ,  DULLA KALYANA CHAKRAVARTHI ,  ALVES DE INDA MARCIA ,  HOFFMANN RALF

Inventor： VERHAEGH WILHELMUS FRANCISCUS JOHANNES ,  VAN DE STOLPE ANJA ,  VAN OOIJEN HENDRIK JAN ,  DULLA KALYANA CHAKRAVARTHI ,  ALVES DE INDA MARCIA ,  HOFFMANN RALF

IPC: C12Q1/68 ,  G06F19/24

CPC classification number: G06F19/12 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158 ,  G06F19/18 ,  G06F19/20 ,  G06F19/24 ,  C12Q2537/165

Abstract: The present application mainly relates to specific methods for inferring activity of one or more cellular signaling pathway(s) in tissue of a medical subject based at least on the expression level(s) of one or more target gene(s) of the cellular signaling pathway(s) measured in an extracted sample of the tissue of the medical subject, an apparatus comprising a digital compressor configured to perform such methods and a non- transitory storage medium storing instructions that are executable by a digital processing device to perform such methods.

Abstract translation: 本申请主要涉及至少基于细胞信号的一个或多个目标基因的表达水平来推断医学对象组织中的一种或多种细胞信号传导途径的活性的具体方法 在医学对象的组织的提取样本中测量的路径，包括被配置为执行这种方法的数字压缩器和存储可由数字处理设备执行以执行这些方法的指令的非临时存储介质的设备。

公开(公告)号：WO2012177945A3

公开(公告)日：2013-02-28

申请号：PCT/US2012043640

申请日：2012-06-21

Applicant: CHILDRENS HOSP MEDICAL CENTER ,  ROTHENBERG MARC E ,  WEN TING

Inventor： ROTHENBERG MARC E ,  WEN TING

IPC: C12Q1/68 ,  G01F17/00 ,  G01N33/68

CPC classification number: G06F19/3431 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/158 ,  G01N33/49 ,  G01N33/493 ,  G01N33/6893 ,  G01N2800/06 ,  G01N2800/14 ,  G01N2800/24 ,  G01N2800/52 ,  G01N2800/56 ,  G06F19/20 ,  G16H50/30

Abstract: Embodiments of the invention are directed to methods of diagnosing eosinophilic esophagitis (EoE), or remission therefrom in a subject, wherein the methods include applying a sample from the subject to a diagnostic panel that contains selected markers for EoE, analyzing to obtain relatedness information relative to an EoE cohort and making a determination as to the EoE status of the subject, wherein an analysis indicating grouping with an EoE cohort or a quantitative score similar to that of an EoE cohort are indicative of EoE in the subject. Embodiments of the invention are also directed to methods of monitoring the pathological development or medical prognosis of EoE in a subject.

Abstract translation: 本发明的实施方案涉及在受试者中诊断嗜酸粒细胞性食管炎（EoE）或其缓解的方法，其中所述方法包括将来自受试者的样品应用于包含用于EoE的所选标记物的诊断板，分析以获得相关性信息 对EoE队列进行确定并确定所述受试者的EoE状态，其中指示使用EoE队列分组的分析或与EoE队列类似的定量分数的分析指示所述对象中的EoE。 本发明的实施方案还涉及监测受试者中EoE的病理学发展或医学预后的方法。

公开(公告)号：WO2012149107A3

公开(公告)日：2013-01-17

申请号：PCT/US2012035120

申请日：2012-04-26

Applicant: SELVENTA INC

Inventor： CATLETT NATALIE ANNE LEECH ,  DRUBIN DAVID ALAN ,  ELLISTON KEITH OWEN ,  KENNEY RENEE MARIE DEEHAN ,  MACORITTO MICHAEL PAUL

IPC: A61B5/00 ,  G06Q50/24

CPC classification number: G06Q50/22 ,  G06F19/18 ,  G06F19/20 ,  G06Q30/0204 ,  G16H10/20

Abstract: A method of stratifying a set of disease-exhibiting patients prior to clinical trial of a target therapy begins by using a molecular footprint derived from a knowledgebase and other patient data to identify genes that are differentially expressed in a direction consistent with increase in the target activity. Therapeutic target "signaling strength" in individual patients of the set is then assessed using the genes identified and a strength algorithm. Based on their therapeutic target signaling strength, the set of disease- exhibiting patients are then stratified along a continuum. One or more gene expressions or other biomarkers may be specified for use in categorizing other disease-exhibiting patient populations. Alternative therapeutic targets are analyzed with respect to the likely non-responders, as evidenced by their differential signaling strength.

Abstract translation: 在目标治疗临床试验之前分层一组疾病展现患者的方法开始于使用来自知识库和其他患者数据的分子足迹来鉴定与靶活性增加一致的方向上差异表达的基因 。 然后使用所鉴定的基因和强度算法评估该组个体患者的治疗靶标“信号强度”。 根据其治疗目标信号强度，将一组疾病展现患者沿着连续统一层分层。 可以指定一种或多种基因表达或其他生物标志物用于对其他疾病展示患者群体进行分类。 对于可能的无应答者，分析替代治疗目标，如其差异信号强度所证明的。

公开(公告)号：WO2012174271A2

公开(公告)日：2012-12-20

申请号：PCT/US2012/042502

申请日：2012-06-14

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  MIRSKY, Ethan ,  TEMME, Karsten ,  VOIGT, Chris ,  ZHAO, Dehua

Inventor： MIRSKY, Ethan ,  TEMME, Karsten ,  VOIGT, Chris ,  ZHAO, Dehua

IPC: C12N15/09 ,  C12N15/31 ,  C12N15/63

CPC classification number: C12N15/635 ,  C07K14/255 ,  C07K14/26 ,  C12N9/0095 ,  C12N15/52 ,  C12N15/63 ,  C12N15/67 ,  C12N15/74 ,  C12Y118/06001 ,  G06F19/20 ,  G06F19/22

Abstract: Methods for making synthetic gene clusters are described.

Abstract translation: 描述制备合成基因簇的方法。

公开(公告)号：WO2012149607A1

公开(公告)日：2012-11-08

申请号：PCT/AU2012/000475

申请日：2012-05-03

Applicant: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  MENTAL HEALTH RESEARCH INSTITUTE ,  EDITH COWAN UNIVERSITY ,  NATIONAL AGEING RESEARCH INSTITUTE ,  BURNHAM, Samantha, C. ,  FAUX, Noel ,  LAWS, Simon, M.

Inventor： BURNHAM, Samantha, C. ,  FAUX, Noel ,  LAWS, Simon, M.

IPC: G01N33/68 ,  G01N33/96

CPC classification number: G01N33/6896 ,  G01N2333/4709 ,  G01N2800/2821 ,  G01N2800/60 ,  G06F19/20

Abstract: The present invention provides methods for predicting whether a subject will develop a disease capable of affecting cognitive function. More specifically, the present invention relates to the predictive detection of neurological diseases in a subject. The methods and systems provided enable a quantitative assessment and theoretical predictions of neocortical amyloid loading or amyloid beta levels based on the measurement of biomarkers in biological fluids that will provide an indication of whether a subject is likely to develop a neurological disease, such as Alzheimer's disease (AD).

Abstract translation: 本发明提供了预测受试者是否会发展能够影响认知功能的疾病的方法。 更具体地，本发明涉及对象中神经系统疾病的预测检测。 所提供的方法和系统能够基于生物流体中的生物标志物的测量来定量评估和理论预测新皮质淀粉样蛋白负荷或淀粉样蛋白β水平，这将提供受试者是否可能发展成神经系统疾病，如阿尔茨海默病 （广告）。

公开(公告)号：WO2012148477A1

公开(公告)日：2012-11-01

申请号：PCT/US2011/065291

申请日：2011-12-15

Applicant: CELLULAR RESEARCH, INC. ,  FODOR, Stephen, P.A. ,  FU, Glenn, K.

Inventor： FODOR, Stephen, P.A. ,  FU, Glenn, K.

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity counting of individual molecules by stochastic labeling of a identical molecules in mixtures of molecules by attachment of a unique label-tags from a diverse pool of label tags to confer uniqueness to otherwise identical or indistinguishable events. Individual occurrences of target molecules randomly choose from a non- depleting reservoir of diverse label-tags. Labeled molecules may be detected by hybridization or sequencing based methods. Molecules that would otherwise be identical in information content are labeled to create a separately detectable product that can be distinctly detected. The disclosed stochastic transformation methods reduce the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed label-tags are present. The methods may be used, for example, to count a given species of molecule within a sample.

Abstract translation: 公开了组合物，方法和试剂盒，用于通过从不同标签标签池中附加独特的标签标签随机标记分子混合物中的相同分子来对各个分子进行高灵敏度计数，以赋予其他相同或不可区分的事件的唯一性 。 目标分子的个体出现随机地从不同标签标签的非耗尽储层中选择。 标记的分子可以通过杂交或基于测序的方法来检测。 否则在信息内容中相同的分子被标记以产生可以被清楚地检测到的可单独检测的产物。 所公开的随机转换方法减少了从定位和识别相同分子之一计数分子的问题到检测是否存在预编程标签标签的一系列二进制数字问题。 该方法可以用于例如计数样品中的给定种类的分子。