Abstract:
Die Erfindung betrifft ein Verfahren zur Verbindung von Komponenten einer mikrofluidischen Flusszelle, insbesondere zur Einbindung von Bauteilen (3) in eine Tragstruktur (1) der Flusszelle. Das erfindungsgemäße Verfahren ist dadurch gekennzeichnet, dass zwischen den zu verbindenden Komponenten (1, 3) ein Spaltraum (5) gebildet wird, der Spaltraum (5) mit einem Lösungsmittel (7) gefüllt wird, sich in dem Lösungsmittel (7) Material (6) von wenigstens einer der den Spaltraum (5) begrenzenden Komponenten (1,3) auflöst und dass dieses Material (6) nach Verdunsten des Lösungsmittels (7) den Spaltraum (5) in seiner Breite vollständig und seiner Höhe teilweise ausfüllt. Die Erfindung betrifft ferner eine nach dem Verfahren hergestellte Flusszelle.
Abstract:
Techniques and devices are provided related to insert assemblies that may be used in conjunction with microfluidic devices. In one embodiment, the insert assemblies include a functional material, such as a solid stationary phase, that may be coupled to a microfluidic pathway via the insert assembly. In this manner, solid stationary phase materials that may be challenging to directly apply to a microfluidic device may be separately enclosed inside the insert element prior to assembly into a microfluidic device, such as a microfluidic chip.
Abstract:
Methods for preparing low volume microfluidic devices are disclosed along with the resulting microfluidic devices, the use of these microfluidic devices, and kits for forming the microfluidic devices of the present invention.
Abstract:
A method of fabricating a microwell in an array structure is disclosed herein. The array structure includes a plurality of field effect transistors (FETs), where each FET has a gate structure. The method includes disposing a titanium nitride (TiN) layer on at least one conductive layer coupled to the gate structure of at least one FET. A insulation layer is disposed on the array structure, where the insulation layer lies above the TiN layer. Further, an opening above the gate structure of the at least one FET is etched to remove the insulation layer above the gate structure and to expose the TiN layer. A microwell with at least one sidewall formed from the insulation layer and with a bottom surface formed from the TiN layer is a result of the etching process. The gate structure is specified as a floating gate structure and the FET is an ISFET.
Abstract:
A microfluidic assay device that defines a micro-fluidic flow channel (44) having a flow axis, in which a series of discrete, axially-spaced apart, transparent hollow flow elements (32) are secured in fixed position, each flow element having at least one axially-extending flow passage through its interior, assay capture agent fixed to the interior surface of the elements for capture of an analyte in liquid flowing through the interior of the flow elements, the device constructed to enable light to be transmitted out of the elements for reading of fluorescence from captured analyte, wherein: the exterior axially-extending surfaces of the flow elements are free of active capture agent, while at least part of the interior surfaces carry deposits of active capture agent exposed to flow through the elements.
Abstract:
The invention relates to a sensor carrier comprising a flexible film (1) comprising a plurality of functionalized sensor elements (4) each being formed by a functional layer, wherein the functional layers are located on the same surface of the film (1) within a window area (6), wherein a region (7) of each of the functional layers of the sensor elements (4) is functionalized, and wherein one or more sensor compounds (5) are arranged or located in the respective functionalized regions (7) of the sensor elements (4).
Abstract:
Microporous membranes formed in a microfluidic device, and methods of manufacture. A method comprises the steps of etching a plurality of pillars in a microfluidic chamber, applying a first polymer material layer, applying a photoresist layer, exposing the photoresist layer to radiation to cross-link it to the microfluidic chamber, masking the photoresist layer with a porous mask, exposing the top layer of the masked photoresist layer to radiation to form a porous membrane layer of cross-linked photoresist material, removing the non-exposed photoresist material from under the porous membrane layer, drying the porous membrane layer, and removing the first polymer material from under the porous membrane layer.
Abstract:
In assembling a portion of a microfluidic device by conducting bonding action by contacting faces of opposed bondable materials, one comprising a flexible sheet, the method, while maintaining continual contact of the faces in a region R1 until bonding is completed, of employing repeated make-and-break-contact manufacturing protocol on a second region R2 of the contacted faces of the bondable materials, thereby over time neutralizing the tendency for permanent bonds to form in that region R2, thus to enable making and breaking actuated movements of the second region R2 of the flexible sheet relative to the portion of the other material that it opposes.
Abstract:
A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.