-
公开(公告)号：WO2008082670A3
公开(公告)日：2008-11-27
申请号：PCT/US2007026523
申请日：2007-12-27
Applicant: WAKO PURE CHEM IND LTD , WADA HENRY G , KAWABATA TOMOHISA , BOUSSE LUC
Inventor： WADA HENRY G , KAWABATA TOMOHISA , BOUSSE LUC
CPC classification number: G01N27/447 , G01N21/274 , G01N21/278 , G01N33/582 , G01N2001/2893
Abstract: Internal standard concentration is normalized as an independent parameter through measuring a buffer conductance in a system with characterized reagents and in a defined assay device. The conductivity of a fresh internal standard solution is measured whether in isolation or as part of a system that is subject to a uniform variation such as evaporation or condensation. Collected conductance measurements may be used to normalize assay signals that are dependent upon fluidic channel dimensions, such as fluorescence assay signal measurement.
Abstract translation: 通过在具有特征试剂的系统中并在限定的测定装置中测量缓冲液电导，将内标浓度标准化为独立参数。 新鲜内标溶液的电导率可以单独测量，也可以作为蒸发或冷凝等均匀变化系统的一部分进行测量。 收集的电导测量结果可用于标准化取决于流体通道尺寸的测定信号，例如荧光测定信号测量。
-
公开(公告)号：WO2004094994A3
公开(公告)日：2005-06-23
申请号：PCT/US2004006918
申请日：2004-03-04
Applicant: PREDICANT BIOSCIENCES INC , HELLER JONATHAN , STULTS JOHN , SRINIVASAN UTHARA , BOUSSE LUC , ZHAO MINGQI , NI JING
Inventor： HELLER JONATHAN , STULTS JOHN , SRINIVASAN UTHARA , BOUSSE LUC , ZHAO MINGQI , NI JING
IPC: F15C1/04 , F16L58/04 , G01N20060101 , H01J49/04 , H01J49/16
CPC classification number: H01J49/0018 , H01J49/165 , Y10T137/0402 , Y10T137/2082 , Y10T137/2191 , Y10T137/2224 , Y10T137/7036 , Y10T436/2575
Abstract: Microfluidic devices provide substances to a mass spectrometer. The microfluidic devices include first and second surfaces, at least one microchannel formed by the surfaces, and an outlet at an edge of the surfaces. Some embodiments also include a tip surface with one or more surface features for helping guide substances from the outlet of the device toward a mass spectrometer. In some embodiments, the surface feature(s) includes a groove, which may be hydrophilic along all or part of its length. Hydrophilic surfaces and/or hydrophobic surfaces may also help guide substances out of the outlet and/or toward the mass spectrometer. In some embodiments, the outlet and/or the tip surface is recessed back from an adjacent portion of the edge. A source of electrical potential can help move substances through the microchannel, separate substances and/or provide electrospray ionization.
Abstract translation: 微流体装置向质谱仪提供物质。 微流体装置包括第一和第二表面，由表面形成的至少一个微通道和表面边缘处的出口。 一些实施例还包括具有一个或多个表面特征的尖端表面，用于帮助将物质从装置的出口引向质谱仪。 在一些实施例中，表面特征包括凹槽，其可以沿其长度的全部或部分亲水。 亲水表面和/或疏水表面还可以帮助将物质引出出口和/或朝向质谱仪。 在一些实施例中，出口和/或尖端表面从边缘的相邻部分向后凹陷。 电势源可以帮助物质通过微通道，分离物质和/或提供电喷雾电离。
-
公开(公告)号：WO2004094994A2
公开(公告)日：2004-11-04
申请号：PCT/US2004/006918
申请日：2004-03-04
Applicant: PREDICANT BIOSCIENCES, INC. , HELLER, Jonathan , STULTS, John , SRINIVASAN, Uthara , BOUSSE, Luc , ZHAO, Mingqi , NI, Jing
Inventor： HELLER, Jonathan , STULTS, John , SRINIVASAN, Uthara , BOUSSE, Luc , ZHAO, Mingqi , NI, Jing
IPC: G01N
CPC classification number: H01J49/0018 , H01J49/165 , Y10T137/0402 , Y10T137/2082 , Y10T137/2191 , Y10T137/2224 , Y10T137/7036 , Y10T436/2575
Abstract: Microfluidic devices provide substances to a mass spectrometer. The microfluidic devices include first and second surfaces, at least one microchannel formed by the surfaces, and an outlet at an edge of the surfaces. Some embodiments also include a tip surface with one or more surface features for helping guide substances from the outlet of the device toward a mass spectrometer. In some embodiments, the surface feature(s) includes a groove, which may be hydrophilic along all or part of its length. Hydrophilic surfaces and/or hydrophobic surfaces may also help guide substances out of the outlet and/or toward the mass spectrometer. In some embodiments, the outlet and/or the tip surface is recessed back from an adjacent portion of the edge. A source of electrical potential can help move substances through the microchannel, separate substances and/or provide electrospray ionization.
Abstract translation: 微流体装置向质谱仪提供物质。 微流体装置包括第一和第二表面，由表面形成的至少一个微通道和表面边缘处的出口。 一些实施例还包括具有一个或多个表面特征的尖端表面，用于帮助将物质从装置的出口引向质谱仪。 在一些实施例中，表面特征包括凹槽，其可以沿其长度的全部或部分亲水。 亲水表面和/或疏水表面还可以帮助将物质引出出口和/或朝向质谱仪。 在一些实施例中，出口和/或尖端表面从边缘的相邻部分向后凹陷。 电势源可以帮助物质通过微通道，分离物质和/或提供电喷雾电离。
-
4.发明申请
公开(公告)号：WO1998000231A1
公开(公告)日：1998-01-08
申请号：PCT/US1997010894
申请日：1997-06-24
Applicant: CALIPER TECHNOLOGIES CORPORATION , PARCE, John, Wallace , KOPF-SILL, Anne, R. , BOUSSE, Luc, J.
Inventor： CALIPER TECHNOLOGIES CORPORATION
IPC: B01J19/00
CPC classification number: G01N33/502 , B01J19/0093 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00853 , B01J2219/00952 , B01J2219/00961 , B01J2219/0097 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L2200/027 , B01L2200/0605 , B01L2200/0647 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2400/0415 , B01L2400/0418 , B01L2400/0487 , G01N27/44791 , G01N33/5008 , G01N33/5011 , G01N33/5064 , G01N33/5091 , G01N33/5097 , G01N33/5304 , G01N33/57415 , G01N35/08 , Y10S366/02 , Y10S435/81 , Y10S435/817 , Y10S436/804 , Y10S436/805 , Y10S436/806 , Y10T436/11 , Y10T436/110833 , Y10T436/113332 , Y10T436/117497 , Y10T436/25
Abstract: The present invention provides microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. The device includes a series of channels (110, 112), and optional reagent channel (114), fabricated into the surface of the substrate. At least one of these channels will typically have very small cross-sectional dimensions, e.g. in the range of from about 0.1 mu m to about 500 mu m. The device also includes reservoirs (104, 106 and 108), disposed and fluidly connected at the ends of the channels (110 and 114). As shown, sample channel (112) is used to introduce the plurality of different test compounds into the device. As such, this channel will generally be fluidly connected to a source of large numbers of separate test compounds that will be individually introduced into the sample channel (112) and subsequently into channel (110).
Abstract translation: 本发明提供了用于进行高通量筛选测定的微流体装置和方法。 特别地，本发明的装置和方法可用于筛选大量不同的化合物，因为它们对多种化学品，优选生物化学体系的影响。 该装置包括一系列通道（110,112）和可选的试剂通道（114），其制造在基板的表面中。 这些通道中的至少一个通常具有非常小的横截面尺寸，例如， 在约0.1μm至约500μm的范围内。 装置还包括在通道（110和114）的端部处设置并流体连接的储存器（104,106和108）。 如图所示，使用样品通道（112）将多种不同的测试化合物引入装置。 因此，该通道通常将流体地连接到将分别引入样品通道（112）并随后进入通道（110）的大量单独测试化合物的源。
-
公开(公告)号：WO2021030353A1
公开(公告)日：2021-02-18
申请号：PCT/US2020/045775
申请日：2020-08-11
Applicant: INTABIO, INC. , GENTALEN, Erik , MACK, Scott , GWERDER, Eric , BOUSSE, Luc
Inventor： GENTALEN, Erik , MACK, Scott , GWERDER, Eric , BOUSSE, Luc
Abstract: Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.
-
Patent Agency Ranking6.发明申请
公开(公告)号：WO2008154036A1
公开(公告)日：2008-12-18
申请号：PCT/US2008/007359
申请日：2008-06-11
Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD. , BOUSSE, Luc , ZHANG, Jian-ping
Inventor： BOUSSE, Luc , ZHANG, Jian-ping
CPC classification number: G01N27/44791 , B01L3/502746 , B01L3/502753 , B01L7/52 , B01L2300/0816 , B01L2400/0421 , G01N27/44721
Abstract: A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10Λ3 times smaller than the hydrodynamic flow resistance in the one or more separation channels The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective
Abstract translation: 微流体装置具有适当的集成结构以进行大容量PCR和终点或实时毛细管电泳检测微流体装置包括具有一定体积核酸的扩增室的底物，设置在基底上的阱，流路 将所述孔和所述腔室连接在所述基底中以允许溶液流过所述腔室，以及设置在所述基底中并连接到所述腔室的一个或多个分离通道，用于分离和检测所述扩增的核酸的一部分所述腔室，所述流动通道 并且所述一个或多个分离通道被构造成使得所述腔室和所述流动通道组合的流体动力学流动阻力比所述一个或多个分离通道中的流体动力学流动阻力小至少10〜3倍。所述微流体装置可以实现 检测灵敏度非常高，而且成本效益高
-
公开(公告)号：WO2008121803A1
公开(公告)日：2008-10-09
申请号：PCT/US2008/058624
申请日：2008-03-28
Applicant: FUJIFILM CORPORATION , WAKO PURE CHEMICAL INDUSTRIES, LTD. , SETO, Yoshihiro , BOUSSE, Luc , LI, Chen
Inventor： SETO, Yoshihiro , BOUSSE, Luc , LI, Chen
IPC: B08B9/00
CPC classification number: B08B9/0321 , B08B9/035
Abstract: To improve cleansing quality of wall surfaces of micro-flow channels in a micro-flow channel cleansing method. During cleansing of wall surfaces of micro-flow channels having at least one branching channel, by causing cleansing fluid to flow therethrough, the cleansing fluid is caused to flow through the at least one branching channel such that there is no residual fluid on the wall surfaces thereof.
Abstract translation: 以微流通道清洗方式提高微流通道壁表面的清洁质量。 在清洁具有至少一个分支通道的微流动通道的壁表面之间，通过使清洁流体流过其中，使净化流体流过至少一个分支通道，使得在壁表面上不存在残留流体 它们。
-
公开(公告)号：WO2008082670A2
公开(公告)日：2008-07-10
申请号：PCT/US2007/026523
申请日：2007-12-27
Applicant: WAKO PURE CHEMICHAL INDUSTRIES, LTD. , WADA, Henry G. , KAWABATA, Tomohisa , BOUSSE, Luc
Inventor： WADA, Henry G. , KAWABATA, Tomohisa , BOUSSE, Luc
IPC: G01N27/28
CPC classification number: G01N27/447 , G01N21/274 , G01N21/278 , G01N33/582 , G01N2001/2893
Abstract: Internal standard concentration is normalized as an independent parameter through measuring a buffer conductance in a system with characterized reagents and in a defined assay device. The conductivity of a fresh internal standard solution is measured whether in isolation or as part of a system that is subject to a uniform variation such as evaporation or condensation. Collected conductance measurements may be used to normalize assay signals that are dependent upon fluidic channel dimensions, such as fluorescence assay signal measurement.
Abstract translation: 通过在具有特征的试剂和定义的测定装置的系统中测量缓冲电导，将内标浓度归一化为独立参数。 无论是隔离还是作为受到均匀变化如蒸发或冷凝的系统的一部分，测量新鲜内标溶液的电导率。 收集的电导测量可用于标准化依赖于流体通道尺寸的测定信号，例如荧光测定信号测量。
-
公开(公告)号：WO2007089884A8
公开(公告)日：2008-02-07
申请号：PCT/US2007002721
申请日：2007-01-31
Applicant: MICROCHIP BIOTECHNOLOGIES INC , NGUYEN MICHAEL , BOUSSE LUC
Inventor： NGUYEN MICHAEL , BOUSSE LUC
CPC classification number: C07K1/26 , G01N27/44791 , Y10T436/2575
Abstract: The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.
Abstract translation: 本文的发明提供了改进的样品注射系统和相关方法来产生具有对称通道构型的微流体装置，其可以产生相对较大的样品体积。 本发明的一个实施方案提供了具有不同几何形状的微流体结构，其从样品负载通道和样品废物通道的角度对称，其基本上消除了时间偏移和通常与双T样品形成技术相关的其它问题的问题。 因此提供了分离注射方法和样品塞形成的相关方法。
-
公开(公告)号：WO2007089884A2
公开(公告)日：2007-08-09
申请号：PCT/US2007/002721
申请日：2007-01-31
Applicant: PATHWORK DIAGNOSTICS, INC. , NGUYEN, Michael , BOUSSE, Luc
Inventor： NGUYEN, Michael , BOUSSE, Luc
CPC classification number: C07K1/26 , G01N27/44791 , Y10T436/2575
Abstract: The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.
Abstract translation: 本文的发明提供了改进的样品注射系统和相关方法，以产生具有对称通道配置的微流体装置，其可产生相对大的样品体积。 本发明的一个实施例提供具有不同几何形状的微流体结构，其从样品装载通道和样品废物通道的角度来看是对称的，其基本上消除了时间偏移和通常与双T样品形成技术相关的其他问题的问题。 因此提供了分流注射方法和相关的样品塞形成方法。
-
-
-
-
-
-
-
-
-
Search Results
23
records
(took 0.011 seconds)
