公开(公告)号：WO1998000707A1

公开(公告)日：1998-01-08

申请号：PCT/US1997012930

申请日：1997-07-03

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  CHOW, Calvin, Y., H. ,  PARCE, J., Wallace

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: G01N27/26

CPC classification number: G01N27/44791 ,  G01N27/44713 ,  G01N27/44752 ,  G01N27/44773 ,  Y10S366/02 ,  Y10T29/49002

Abstract: In a microfluidic system (partially shown by element 178 in the figure and elements thereupon) using electrokinetic forces, the present invention uses electrical current or electrical parameters, other than voltage, to control the movement of fluids through the channels of the system. Time-multiplexed power supplies (200 and 202) also provide further control over fluid movement by varying the voltage on an electrode connected to a fluid reservoir of the microfluidic system, by varying the duty cycle during which the voltage is applied to the electrode, or by a combination of both. A time-multiplexed power supply can also be connected to more than one electrode for a saving in cost.

Abstract translation: 在使用电动力的微流体系统（图中由元件178部分地示出）上，本发明使用电压以外的电流或电气参数来控制流体通过系统的通道的运动。 时间复用电源（200和202）还通过改变连接到微流体系统的流体存储器的电极上的电压来改变对电极施加电压的占空比，来进一步控制流体运动，或者 通过两者的结合。 时间复用电源也可以连接到多个电极以节省成本。

公开(公告)号：WO1998000231A1

公开(公告)日：1998-01-08

申请号：PCT/US1997010894

申请日：1997-06-24

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  PARCE, John, Wallace ,  KOPF-SILL, Anne, R. ,  BOUSSE, Luc, J.

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: B01J19/00

CPC classification number: G01N33/502 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/0097 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0487 ,  G01N27/44791 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5064 ,  G01N33/5091 ,  G01N33/5097 ,  G01N33/5304 ,  G01N33/57415 ,  G01N35/08 ,  Y10S366/02 ,  Y10S435/81 ,  Y10S435/817 ,  Y10S436/804 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/113332 ,  Y10T436/117497 ,  Y10T436/25

Abstract: The present invention provides microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. The device includes a series of channels (110, 112), and optional reagent channel (114), fabricated into the surface of the substrate. At least one of these channels will typically have very small cross-sectional dimensions, e.g. in the range of from about 0.1 mu m to about 500 mu m. The device also includes reservoirs (104, 106 and 108), disposed and fluidly connected at the ends of the channels (110 and 114). As shown, sample channel (112) is used to introduce the plurality of different test compounds into the device. As such, this channel will generally be fluidly connected to a source of large numbers of separate test compounds that will be individually introduced into the sample channel (112) and subsequently into channel (110).

Abstract translation: 本发明提供了用于进行高通量筛选测定的微流体装置和方法。 特别地，本发明的装置和方法可用于筛选大量不同的化合物，因为它们对多种化学品，优选生物化学体系的影响。 该装置包括一系列通道（110,112）和可选的试剂通道（114），其制造在基板的表面中。 这些通道中的至少一个通常具有非常小的横截面尺寸，例如， 在约0.1μm至约500μm的范围内。 装置还包括在通道（110和114）的端部处设置并流体连接的储存器（104,106和108）。 如图所示，使用样品通道（112）将多种不同的测试化合物引入装置。 因此，该通道通常将流体地连接到将分别引入样品通道（112）并随后进入通道（110）的大量单独测试化合物的源。

公开(公告)号：WO1998002728A1

公开(公告)日：1998-01-22

申请号：PCT/US1997012262

申请日：1997-07-14

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  PARCE, J., Wallace

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: G01N21/00

CPC classification number: G01N27/44791 ,  G01N27/44721 ,  Y10S366/02

Abstract: The present invention provides a microfluidic system (100) for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel (110) in the microfluidic system are received by the photodetector (130). The intensity of light received by the photodetector is modulated by the movement of species bands (123) through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band (123) is determined and the identification of the species is made by its electrophoretic mobility in the channel (110).

Abstract translation: 本发明提供了用于在化学，生物化学，生物技术，分子生物学和许多其他领域的材料的快速，准确和低成本电泳分析的微流体系统（100）。 沿着微流体系统中的通道（110）的周期性间隔的区域的光由光电检测器（130）接收。 由光电检测器接收的光的强度由物理带（123）在电泳力下通过通道的移动来调制。 通过傅立叶分析，确定每个物种带（123）的速度，并通过其在通道（110）中的电泳迁移率来确定物种的鉴定。

公开(公告)号：WO1998022811A1

公开(公告)日：1998-05-28

申请号：PCT/US1997021024

申请日：1997-11-18

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  PARCE, John, Wallace ,  KOPF-SILL, Anne, R. ,  BOUSSE, Luc, J.

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: G01N27/26

CPC classification number: B01L3/50273 ,  B01L3/5025 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0688 ,  G01N27/44791 ,  Y10T436/2575

Abstract: The present invention generally provides microfluidic devices (200) and systems that utilize electrokinetic material transport systems to selectively control and direct the transport of materials through and among complex arrangements of integrated microscale channels (for example, 202 and 208 + 2n, where n = 0, 1, ...8) disposed within integrated body structures.

Abstract translation: 本发明通常提供微流体装置（200）和利用电动材料输送系统来选择性地控制和指导材料通过综合微量通道（例如，202和208 + 2n，其中n = 0 ，1，... 8）。

公开(公告)号：WO1998005424A1

公开(公告)日：1998-02-12

申请号：PCT/US1997013178

申请日：1997-07-31

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  CHOW, Calvin, Y., H.

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: B01L03/00

CPC classification number: B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2400/0415 ,  G01N2035/00326 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: An analytical or preparatory system comprises as a base unit (12), an adapter (14), and a substrate (16). The adapter (14) is attached to an attachment region on the base unit (12), and the substrate (16) is attached to an attachment region on the adapter (14). The adapter (14) permits the base unit (12) to be interfaced with a wide variety of different substrates (16) to perform chemical and biological analytical analyses and preparatory procedures.

Abstract translation: 分析或准备系统包括基本单元（12），适配器（14）和基板（16）。 适配器（14）附接到基座单元（12）上的附接区域，并且基板（16）附接到适配器（14）上的附接区域。 适配器（14）允许基座单元（12）与各种不同的基板（16）接合，以进行化学和生物分析分析和准备程序。

公开(公告)号：WO1998000705A1

公开(公告)日：1998-01-08

申请号：PCT/US1997010963

申请日：1997-06-25

Applicant: CALIPER TECHNOLOGIES CORPORATION ,  PARCE, J., Wallace ,  KNAPP, Michael, R.

Inventor： CALIPER TECHNOLOGIES CORPORATION

IPC: G01N27/26

CPC classification number: B01F13/0071 ,  B01F13/0084 ,  B01F13/0086 ,  B01J19/0093 ,  B01J2219/00891 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  F04B17/00 ,  F04B19/006 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791 ,  H02N11/006 ,  Y10S366/02 ,  Y10T436/113332 ,  Y10T436/2575

Abstract: A channel (140) is divised into portions (142, 144). The sidewalls of each channel portion (142, 144) have surface charges of opposite polarity. The two channel portions (142, 144) are physically connected together by a salt bridge (133), such as a glass frit or gel layer. The salt bridge (133) separates the fluids in channel (140) from an ionic fluid reservoir (135). To impart electroosmotic and electrophoretic forces along the channel (140) between parts A and B, respectively. Additionally, a third electrode (137) is placed in the reservoir (135).

Abstract translation: 通道（140）被分成部分（142,144）。 每个通道部分（142,144）的侧壁具有相反极性的表面电荷。 两个通道部分（142,144）通过盐桥（133）物理连接在一起，例如玻璃料或凝胶层。 盐桥（133）将通道（140）中的流体与离子流体储存器（135）分离。 分别沿着部分A和B之间的通道（140）施加电渗和电泳力。 此外，第三电极（137）被放置在储存器（135）中。