公开(公告)号：WO2016010975A2

公开(公告)日：2016-01-21

申请号：PCT/US2015040296

申请日：2015-07-14

Applicant: ILLUMINA INC

Inventor： BOYANOV BOYAN ,  MANDELL JEFFREY G ,  BAI JINGWEI ,  GUNDERSON KEVIN L ,  CHEN CHENG-YAO ,  PERBOST MICHEL

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01J2219/00653 ,  B01J2219/00722 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/4145 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2563/116 ,  C12Q2565/107 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2521/101

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Abstract translation: 一种核酸测序的方法。 该方法可以包括以下步骤：（a）提供连接至固体支持物电荷传感器的聚合酶; （b）提供一个或多个核苷酸，由此可以通过电荷传感器检测核苷酸的存在; 和（c）检测核苷酸掺入与模板核酸互补的新生链中。

公开(公告)号：WO2015187868A8

公开(公告)日：2017-01-05

申请号：PCT/US2015034053

申请日：2015-06-03

Applicant: ILLUMINA INC

Inventor： BOHM SEBASTIAN ,  ARAVANIS ALEX ,  HSIAO ALEXANDER ,  JAVANMARDI BEHNAM ,  KHURANA TARUN ,  TRAN HAI QUANG ,  AGHABABAZADEH MAJID ,  BOWEN M SHANE ,  BOYANOV BOYAN ,  BUERMANN DALE

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Abstract translation: 用于进行指定反应的系统和方法，其包括具有样品通道的流体网络，反应室和储存器。 样品通道与样品端口流通。 该系统还包括具有流动通道并被配置为在第一和第二阀位置之间旋转的旋转阀。 当旋转阀处于第一阀位置时，流动通道流体耦合反应室和样品通道，并且当旋转阀处于第二阀位置时，流体流动地联接储存器和反应室。 当旋转阀处于第一阀位置时，泵组件将生物样品流向反应室，当旋转阀处于第二阀位置时，泵组件将反应组分从储存器流向反应室。

公开(公告)号：WO2015187868A3

公开(公告)日：2016-01-28

申请号：PCT/US2015034053

申请日：2015-06-03

Applicant: ILLUMINA INC

Inventor： BOHM SEBASTIAN ,  ARAVANIS ALEX ,  HSIAO ALEXANDER ,  JAVANMARDI BEHNAM ,  KHURANA TARUN ,  TRAN HAI QUANG ,  AGHABABAZADEH MAJID ,  BOWEN M SHANE ,  BOYANOV BOYAN ,  BUERMANN DALE

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Abstract translation: 用于进行指定反应的系统和方法，其包括具有样品通道的流体网络，反应室和储存器。 样品通道与样品端口流通。 该系统还包括具有流动通道并被配置为在第一和第二阀位置之间旋转的旋转阀。 当旋转阀处于第一阀位置时，流动通道流体耦合反应室和样品通道，并且当旋转阀处于第二阀位置时，流体流动地联接储存器和反应室。 当旋转阀处于第一阀位置时，泵组件将生物样品流向反应室，当旋转阀处于第二阀位置时，泵组件将反应组分从储存器流向反应室。